PubMed · 10543870
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E M van der Velden/Samderubun, W H Beekhuis, B H Drost, L D den Dulk. 1999. Treating iris defects.. https://pubmed.ncbi.nlm.nih.gov/10543870/
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Pectobacterium brasiliense 1692 (Pbr1692) is a necrotrophic pathogen that infects many crops such as potatoes and ornamental plants and derives nutrients from degraded plant tissue. Previous studies have identified Pbr1692 genes required for ecological fitness and virulence, however there is a lack of information on nutrient utilization in Pbr1692. Carbon source utilization profiling in Pbr1692 could provide a platform to decipher its metabolic flexibility and adaptation. This study assessed the nutrient utilization of Pbr1692 in different carbon sources, using Biolog Phenotypic Microarray (PM). An array of carbon sources utilized by Pbr1692 were identified, 32 carbohydrates and 8 carboxylic acids were among the preferred carbon nutrients utilized by Pbr1692. The PM results also revealed that the citric acid cycle, amino acid metabolism, and pentose phosphate metabolic pathways might be used to produce energy for Pbr1692. In addition, growth of Pbr1692 cells in minimal medium supplemented with citric acid, glucose, and aspartic acid retained the typical rod shape, suggesting that nutrient variation did not influence Pbr1692 cell morphology adaptation. This study provides an understanding on the adaptation of Pbr1692 and lays a foundation for understanding carbon metabolism of Pbr1692.
Simple gas chromatographic-mass spectrometric determination of hydrophilic organic compounds in environmental water was developed. A cartridge containing activated carbon fiber felt was made by way of trial and was evaluated for solid-phase extraction of the compounds in water. The hydrophilic compounds investigated were acrylamide, N,N-dimethylacetamide, N,N-dimethylformamide, 1,4-dioxane, furfural, furfuryl alcohol, N-nitrosodiethylamine and N-nitrosodimethylamine. Overall recoveries were good (80-100%) from groundwater and river water. The relative standard deviations ranged from 4.5 to 16% for the target compounds. The minimum detectable concentrations were 0.02 to 0.03 microg/l. This method was successfully applied to several river water samples.
Anoxic degradation of sedimentary biogenic debris using closed incubation experiments was studied at two sampling stations in the Gulf of Trieste (northern Adriatic). Production rates of dissolved inorganic carbon (DIC), NH4+, PO(4)3- and dissolved Si (dSi), and reduction rates of SO(4)2- were measured and anoxic mineralization rates were modeled using a first order G-model and multi-G approach. The depth profiles of these rates revealed an exponential decrease indicating that the largest fraction of mineralization of biogenic debris and SO(4)2- reduction occurs in the surficial sediment layer and on the sediment surface. Comparing the depth-integrated anoxic mineralization rates at both stations with benthic fluxes of DIC, NH4+, PO(4)3- and dSi measured at the in situ temperature in the dark, it appears that the DIC and PO(4)3- fluxes are higher because the mineralization mostly occurs at the sediment-water interface, and that besides SO(4)2- reduction, other electron acceptors are involved in the organic matter decomposition pathway in these surficial sediments. The NH4+ production was higher than the benthic fluxes because of NH4+ oxidation. The production of dSi was in good agreement with benthic fluxes implying that temperature is the main factor of dSi production and benthic fluxes in these sediments.