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PubMed · 10521350

Sequencing complex polysaccharides.

G Venkataraman·Z Shriver·R Raman·R Sasisekharan
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Abstract

Although rapid sequencing of polynucleotides and polypeptides has become commonplace, it has not been possible to rapidly sequence femto- to picomole amounts of tissue-derived complex polysaccharides. Heparin-like glycosaminoglycans (HLGAGs) were readily sequenced by a combination of matrix-assisted laser desorption ionization mass spectrometry and a notation system for representation of polysaccharide sequences. This will enable identification of sequences that are critical to HLGAG biological activities in anticoagulation, cell growth, and differentiation.

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Carbohydrate SequenceDisaccharidesGlycosaminoglycansHeparinHeparin LyaseMolecular Sequence DataMolecular WeightNitrous AcidOligosaccharidesPolysaccharide-LyasesSequence AnalysisSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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BibTeXRIS

G Venkataraman, Z Shriver, R Raman, R Sasisekharan. 1999-10-15. Sequencing complex polysaccharides.. https://doi.org/10.1126/science.286.5439.537

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