Selection analysis of single-stranded clones using solid-phase techniques.

T Vener; A Holmberg; J Lundeberg; M Uhlén

doi:10.1016/s1050-3862(98)00035-7

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Selection analysis of single-stranded clones using solid-phase techniques.

T Vener·A Holmberg·J Lundeberg·M Uhlén

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Abstract

An efficient selection analysis of single-stranded DNA fragments using subtractive hybridization on magnetic beads and fluorescence-based fragment analysis is described. This approach facilitates either direct cloning of captured fragments or removal of common clones from cDNA or shot-gun clone libraries.

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Animals DNA, Single-Stranded Humans Mice Nucleic Acid Hybridization Journal Article Research Support, Non-U.S. Gov't

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T Vener, A Holmberg, J Lundeberg, M Uhlén. 1999. Selection analysis of single-stranded clones using solid-phase techniques.. https://doi.org/10.1016/s1050-3862(98)00035-7

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