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Biomedical subjects

Z Zou

Publications and source records attributed to Z Zou.

At least 73 records · Page 4Linked to original sources
PubMed1994

Angiotensin II receptor antagonist TCV-116 induces regression of hypertensive left ventricular hypertrophy in vivo and inhibits the intracellular signaling pathway of stretch-mediated cardiomyocyte hypertrophy in vitro.

BACKGROUND: Previous studies have demonstrated that angiotensin II (Ang II) acts as a growth-promoting factor directly on cardiac myocytes and that angiotensin-converting enzyme inhibitor induces regression of hypertrophied hearts both in experimental animals and in humans. These results suggest that the renin-angiotensin system (RAS) is involved in the formation of left ventricular hypertrophy (LVH). To elucidate the role of RAS in the progression of cardiac hypertrophy, we evaluated the effect of an Ang II receptor antagonist on LVH in spontaneously hypertensive rats (SHRs) and investigated the molecular mechanisms by which antagonizing Ang II receptors reduces cell hypertrophy of myocytes using the in vitro model of mechanical stretch. METHODS AND RESULTS: In the in vivo study, we treated SHRs with the nonpeptide Ang II receptor antagonist TCV-116 (0.1, 1, or 10 mg/kg per day) or hydralazine (10 mg/kg per day). Blood pressure was measured by the tail-cuff method, and wall thickness of left ventricle was serially monitored using M-mode echocardiography. Rats were killed at the age of 13, 17, 21, or 25 weeks, and left ventricular (LV) weight, transverse diameter of cardiomyocytes, relative amount of V3 myosin heavy chain (MHC), and degree of interstitial collagen accumulation were examined. Untreated SHRs progressively developed severe hypertension, but treatment with TCV-116 or hydralazine inhibited the increase in blood pressure. Treatment with TCV-116 reduced LV weight, LV wall thickness, transverse diameter of myocytes, relative amount of V3 MHC, and interstitial fibrosis, whereas treatment with hydralazine slightly prevented an increase in LV wall thickness but did not exert significant reduction in other parameters. In the in vitro study, neonatal rat cardiomyocytes were cultured on deformable silicone dishes and mechanically stretched with or without pretreatment of CV-11974 (an active metabolite of TCV-116), and [3H]phenylalanine incorporation, activity of mitogen-activated protein (MAP) kinase, and c-fos mRNA expression were analyzed. Pretreatment of cultured cardiomyocytes with 10(-7) mol/L CV-11974 inhibited an increase in [3H]phenylalanine incorporation, MAP kinase activity, and c-fos gene expression induced by stretch of cardiomyocytes. CONCLUSIONS: The Ang II receptor antagonist TCV-116 induced regression of cardiac hypertrophy and had cardioprotective effects on hypertrophied myocardium in vivo, and antagonizing Ang II receptors inhibited intracellular signaling of stretch-mediated cardiomyocyte hypertrophy in vitro. These results suggest a crucial role of the cardiac RAS in the development of LVH produced by pressure overload.

Angiotensin II↗
PubMed1994

[Synthesis of new fluorescent reagent and its application in solution fluorescence analysis].

In our laboratory we have designed and synthesized a new fluorescent CGE(N), which has a fluo-rigen and can react with active hydrogen in chemical compounds. We have studied its application in solution fluorescence, thin-layer fluorescence and solid fluorescence analysis. The results have indicated that CGE (N) is a good reagent in analysis. And the example of its application in solution fluorescence analysis is given in this paper.

Colchicine↗
PubMed1993

[The applicability of a new stable fluorescence reagent CGE (N) for TLC measurements and spectrofluorimetry].

A new stable fluorescent reagent, CGE (N), was designed and synthesized in our laboratory. This paper presents the results of using it to detect a compound having aminogroup. Glycine as an example was derived by using CGE (N), the best condition of derivatisation reaction was pH8, T 50 +/- 1 degree C, and time 3-5 h. Spread agent was CHCl3: CH3OH:CH3COOC2H5: iceHAc (1:3:5:1). Rf of glycine-CGE (N) was 0.45, of which fluorescent spectrum Em was 356nm (at Ex = 277nm). The correlation coefficient between the fluorescent scanning area and quantity was over 0.99, the linear relation retained for 24 h, the detection limit 0.24 microgram, and the linear range 0.24-19.2 micrograms. The data revealed that stability, sensitivity and reproducibility were good. These indicate that CGE(N) is a fine fluorescent reagent for compounds having primary amino-group and this method is reliable.

Chromatography, Thin Layer↗
PubMed1992

Sequential quantitative scintigraphy of parotid glands with chronic inflammatory diseases.

A practical, time-saving procedure for sequential quantitative scintigraphy is introduced and 4 parameters chosen from 12 parameters by discriminant analysis are used to evaluate the function of the parotid gland. The examination was performed in 120 cases, including 16 cases with recurrent parotitis in childhood, 33 with chronic obstructive parotitis (COP), 37 with Sjögren's syndrome (SS), 4 with sialadenosis, and 30 normal controls. The scintigraphic findings were analyzed and compared with the histologic findings. The diagnostic value of this method was investigated and scaling for differential diagnosis of COP and SS was established. Scintigraphy is considered to be a useful method for evaluation of parotid function and as a diagnostic aid for SS and COP, especially in patients in whom sialography cannot be performed.

Adolescent↗
PubMed1991

[Clinical study and long-term follow-up observations on the effect of PCV and SV+A in the treatment of duodenal ulcer].

From Aug. 1977 to June 1988, vagotomy had been performed in 238 patients with duodenal ulcer (DU). According to the clinical signs and the result of gastric acid secretion test, (GAST) parietal cell vagotomy (PCV) and selective vagotomy plus antrectomy (SV+A) were performed in 100 and 138 cases respectively. The patients were followed up for 1 to 10 years. 96% and 97% of them belonged to Visick grade I and II respectively. The recurrence rate was 1.96% in PCV group, while no ulcer recurrence was seen in SV+A group, long-term side effect was rare and the nutritional status was quite good. The follow-up data showed that recurrence rate could be greatly reduced if the mode of vagotomy was selected according to results of GAST.

Adolescent↗
PubMed1991

[The qualitative and quantitative analyses of protein with a novel fluorescence agent (EPQS) by fluorescent scanning on solid substrate].

We synthesized a new fluorescence agent, EPQS, which could be soluble in water and was stable in the analytical process. It was characteristic of good fluorescence parameters. Human serum albumin (HSA) was marked with EPQS and purified by the paper electrophoresis dialysis. We determined the fluorescent spectra and excitation spectra of the marked HSA, or EPQS on the cellulose acetate paper by RF-510 fluorescence spectrophotometer-thin-layer chromatoscanner. The former lambda ex = 370nm lambda em = 470nm; the latter lambda ex = 382nm lambda em = 476 nm. Then we touched different quantity of EPQS-HSA upon the cellulose acetate. The fluorescent scanning on the paper disclosed that the relationship between the quantity adopted and the fluorescence intensity is linear. These showed that the protein could be quickly determined qualitatively and quantitatively by this simple method, and EPQS was useful.

Chromatography, Thin Layer↗
PubMed1990

[States of dentine surface and interface between dentine and coupling agent].

In order to research the states of dentine surface and dentine surface primed with coupling agent, we used microscope, transmission electron microscope (TEM) and solid fluorescence method. Observed under microscope, patterning of dentinal tubules and their poles was very explicit; through the view of interface between coupling agent adsorbed and human dentine powder by TEM, it was revealed that the coupling agent was adsorbed onto the dentine surface as if it "grew out" of the dentine surface; through examination of interface between the coupling agent adsorbed and dentine surface by fluorescence spectrum, which differed from that of the coupling agent or dentine surface, i.e., this fluorescence spectrum of interface changed obviously (red shift). This phenomenon indicated that there was a chemical reaction occurring between tooth surface and adsorbed coupling agent. Therefore we can conclude that the three examinations are necessary to provide the argument for establishment of chemical bonding mechanism between dentine and coupling agent.

Adhesives↗