Biomedical subjects
Publications and source records attributed to Z Zou.
Eight cases of Sjögren syndrome in children were presented. Recurrent swelling of salivary glands was shown to be predominant findings in children with Sjögren syndrome. It was first reported that "lace-like" appearance of main duct was of great value in sialographic diagnosis of Sjögren syndrome in children. The differentiation between Sjögren syndrome and recurrent parotitis in children was discussed.
Sialographic follow-up studies of 36 cases of Sjögren's syndrome (SS) were performed with the duration of 5-72 months (mean 25 months). Three groups were divided according to the intenational criteria: secondary group, 14 cases (xerophthalmia, or/and xerostomia, and connective tissue diseases), primary group, 14 cases (xerostomia and xerophthalmia), and possible group, 8 cases (xerophthalmia or xerostomia only). Sialographic findings at follow-up examination included (1) delayed evacuation, which may occur as early as 5 months later; (2) increased punctate sialectasis; (3) decreased punctate sialectasis with globular increase; (4) Punctate, globular, and cavity sialectasis with radiolucent sialolith; and (5) centripetal atrophy. The cases with secondary and possible SS without xerostomia demonstrated little involvement of the parotid gland, without sialectasis. But sialodochitis was shown when radiolucent sialolith formed. The stimulated whole salivary flow rate (SWSFR) was closely related to sialographic appearance, i.e., the cases with lower value of SWSFR showed more sialectasis sialographically, but with higher SWSFR value, and less sialectasis.
The sialograms of 80 cases with Warthin tumor in parotid gland were analysed. The sialographic characteristics were found as follows: irregular arrangement, dilation and narrow of branch ducts which surrounded the tumor, sausagelike change of main ducts just like that seen in obstructive parotitis, and spot-like dilation of the terminal ducts. The comparative study on roentgen-pathology showed that the spot-like dilation of the terminal ducts was related to the dilation of interlobular duct of the gland surrounding the tumor. The roentgen differential diagnosis of Warthin tumor from the aggressive benign tumor and Sjögren syndrome is discussed.
We analysed the results of the central standard lateral tomographic findings and the macroscopic/microscopic observations on the middle portion of the autopsy materials of 84 temporomandibular joints. It was not suitable to differentiate remodeling and osteoarthrosis of the temporomandibular joint only by whether the surface articular zone of the articular cartilage is intact or not. The early changes of osteoarthrosis could occur in the interface of the bone-cartilage except occuring in the articular zone, even though the surface articular zone was intact. Therefore, we suggest that the definition of osteoarthrosis should be wider. Histopathologic observations proved that the definte radiographic signs, such as sclerosis, erosion, pseudocyst, osteophyte and so on showed on the lateral tomographs were credible signs in the diagnosis of osteoarthrosis.
There is increasing evidence that activation of the insulin-like growth factor I (IGF-I) receptor plays a major role in the control of cellular proliferation of many cell types. We studied the mitogenic effects of IGF-I, IGF-II, and epidermal growth factor (EGF) on growth-arrested HT-3 cells, a human cervical cancer cell line. All three growth factors promoted dose-dependent increases in cell proliferation. In untransformed cells, EGF usually requires stimulation by a "progression" factor such as IGF-I, IGF-II, or insulin (in supraphysiologic concentrations) in order to exert a mitogenic effect. Accordingly, we investigated whether an autocrine pathway involving IGF-I or IGF-II participated in the EGF-induced mitogenesis of HT-3 cells. With the RNase protection assay, IGF-I mRNA was not detected. However, IGF-II mRNA increased in a time-dependent manner following EGF stimulation. The EGF-induced mitogenesis was abrogated in a dose-dependent manner by IGF-binding protein 5 (IGFBP-5), which binds to IGF-II and neutralizes it. An antisense oligonucleotide to IGF-II also inhibited the proliferative response to EGF. In addition, prolonged, but not short-term, stimulation with EGF resulted in autophosphorylation of the IGF-I receptor, and coincubations with both EGF and IGFBP-5 attenuated this effect. These data demonstrate that autocrine secretion of IGF-II in HT-3 cervical cancer cells can participate in EGF-induced mitogenesis and suggest that autocrine signals involving the IGF-I receptor occur "downstream" of competence growth factor receptors such as the EGF receptor.
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Elafin is an elastase inhibitor with a unique structure, not related to the serpin family, which includes the neutrophil elastase inhibitor. The gene was identified in this laboratory by subtractive hybridization between RNAs from human mammary tumor-derived cells and cDNAs from normal human mammary epithelial cells. Elafin is consistently expressed in normal mammary epithelial cells, but is down-regulated in most breast tumor cell lines. Restriction fragment analysis detected no gross deletions or rearrangement of the gene in any of the tumor cell lines examined. The elafin gene was cloned, and both the cDNA and the promoter region were sequenced. A major positive upstream promoter element was identified by chloramphenicol acetyltransferase assay and deletion analysis, active in normal cell extracts but not in extracts of tumor cells. These results demonstrate that differential expression of elafin in normal mammary epithelial cells and breast tumor cells is regulated at the transcriptional level. Cell synchronization experiments demonstrated that elafin mRNA is down-regulated in S phase in normal cells. These results suggest that elafin may act as an inhibitor of cell cycle progression.
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Sialographic follow-up studies of 36 cases of Sjogren's syndrome (SS) were performed with the duration of 5-72 months (mean 25 months). Three groups were divided according to the international criteria: secondary group, 14 cases (xerophthalmia, or/and xerostomia, and connective tissue diseases), primary group, 14 cases (xerostomia and xerophthalmia), and possible group, 8 cases (xerophthalmia or xerosptomia only). Sialographic findings at follow-up examination included (1) delayed evacuation, which may occur as early as 5 months later; (2) increased punctate sialectasis; (3) decreased punctate sialectasis with globular increase; (4) punctate, globular, and cavity sialectasis with radiolucent sialolith; and (5) centripetal atrophy. The cases with secondary and possible SS without xerostomia demonstrated little involvement of the parotid gland, without sialectasis. But sialodochitis was shown when radiolucent sialolith formed. The stimulated whole salivary flow rate (SWSFR) was closely related to sialographic appearance, i.e., the cases with lower value of SWSFR showed more siatectasis sialographically, but with higher SWSFR value, and less sialectasis. The relationship between the SS and pyogenic or candidal infection was discussed.
84 cadavers' temporomandibular joints were used to compare the results shown in standard lateral tomographs and Schuller's position films with gross views on the bone and cartilage changes. The results and conclusions were as follows: (1) The positive rates of the radiographic evidences were associated with the degrees of the macroscopic lesions. The more severe the macroscopic lesion, the higher the positive rate of radiographic evidence. (2) The sensitivity of radiographic examination was positively correlated with the extent of the macroscopic lesion. So, the negative predictive value was. However, the specificity and the positive predictive value of the radiographic examination were negatively correlated with macroscopic change index. It has been found that the more the extent of osteoarthrosis changes, the higher the sensitivity of radiographic examination; the higher the negative predictive values, the higher the positive rate of radiographic evidence, and the lower the underdiagnosis rate was. Meanwhile, the more the extent of bone pathological changes in the joint, the lower specificity of the radiographic examination, and the lower the positive predictive values and the misdiagnosis is increased. (3) The radiographic diagnostic value based on the standard latreal tomographs of TMJ, Schuller's position films or based on the both methods was not satisfied. Its underdiagnosis rate was higher. Therefore, we suggest that multiple-layers lateral tomography should be widely used in clinical practice.
Lead contents in six kinds of foodstuff in China were monitored in 1992. Six hundred and three specimens of food were collected from the markets in Beijing, Shanghai, Jiangsu, Sichuan and Guangdong. Results showed that 96.2% of cereals, 84.4% vegetables, 97.6% dairy products, 96.0% eggs and, 100% meat and fish met the national food hygienic standards for lead allowable content. Daily dietary intake of lead of residents in six areas of China was lower than the provisional standard for Daily Allowable Intake of lead set by World Health Organization.
26 cases with Temporomandibular Joint Dysfunction Syndrome (TMJDS) were included in the present study. Fully corrected lateral tomogrames of TMJ in the intercuspid position were taken for all the cases; and fully corrected lateral arthrotomogrames were taken for 19 cases (21 joints) of them. The area and linear measurements of the joint space were made with the aid of computer. The results showed that the position of the condyle in the patients with TMJDS was located in the posterior position of the fossa. Statistics results showed that there was significant difference between the patient group and control group. Although the posterior displacement of the condyle was often associated with the anterior displacement of the disc, it was not always agreement with the anterior disc displacement. So, the exact diagnosis of disc displacement should be still on the basis of arthrography or other examinations, such as CT or MRI.
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A gene encoding a protein related to the serpin family of protease inhibitors was identified as a candidate tumor suppressor gene that may play a role in human breast cancer. The gene product, called maspin, is expressed in normal mammary epithelial cells but not in most mammary carcinoma cell lines. Transfection of MDA-MB-435 mammary carcinoma cells with the maspin gene did not alter the cells' growth properties in vitro, but reduced the cells' ability to induce tumors and metastasize in nude mice and to invade through a basement membrane matrix in vitro. Analysis of human breast cancer specimens revealed that loss of maspin expression occurred most frequently in advanced cancers. These results support the hypothesis that maspin functions as a tumor suppressor.
As a first step to catalogue mRNAs present in mouse embryonal carcinoma F9 cells, 879 clones corresponding to low-abundance mRNAs were selected from among 2,896 randomly picked up clones of undifferentiated F9 cDNA libraries, using DNA probes complementary to poly(A)+RNAs prepared from undifferentiated F9 cells and to ones prepared from mouse fibroblast L cells. Five-hundred and eighty-two of the 879 clones were partially sequenced, and the subsequent homology search revealed that 201 corresponded to 180 known genes or known DNA sequences, which include not only housekeeping genes but also various tissue-specific genes. Interestingly, at least 24 of the 180 genes are development-related genes in mammals. Among these 24, those for midkine (growth and/or differentiation factor) and interferon-beta are reportedly up-regulated, and those for ECA39 (target for c-Myc regulation), REX-1 (zinc finger protein), and OCT-3 (POU-domain transcription factor) are down-regulated during the development of mouse embryonal carcinoma cells. Thirty-seven of the 582 clones matched the 36 previously reported unidentified ESTs (expressed sequence tags) and the remaining 344 corresponded to 329 novel ESTs. Therefore, partial sequencing of F9 cDNA clones corresponding to low-abundance mRNAs in F9 cells not only provides valuable information concerning development-related genes in mammals, but also many novel ESTs useful for studying mammalian genomes.
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