Biomedical subjects

Z Yang

Publications and source records attributed to Z Yang.

At least 199 records · Page 11Linked to original sources

PubMed2001

[Expression of TNF alpha and VCAM-1 in nasal polyps and relation with eosinophil infiltration].

OBJECTIVE: To study the expression of TNF alpha and VCAM-1 in nasal polyps tissue and its significance. METHOD: Paraffin sections of nasal polyps and chronic rhinitis samples were studied with immunohistochemical technique. RESULT: Expression of TNF alpha and VCAM-1 were stranger in nasal polyps tissues than in controls. There was positive relationship between the expression of TNF alpha and that of VCAM-1(r = 0.833), and expression of VCAM-1 coincide with eosinophil infiltration(r = 0.746). CONCLUSION: It is suggested that TNF alpha may up-regulate the expression of VCAM-1 in vessel endothelium, and prompt adhesion and migration of eosinophils, TNF alpha and VCAM-1 may play important role in the pathogenesis of nasal polyps.

Adult↗

PubMed2001

[The impact of lateral rhinotomy on nasal airway].

OBJECTIVE: In order to ascertain the lateral operation and medial maxillectomy affect on nasal airway. METHOD: Report 16 cases of nasal cavity or nasal sinus neoplasm who were therapied by lateral rhinotomy and medial maxillectomy. They were observed by acoustic rhinometer (AR) and CT examination in 1-2.5 years after the surgery. RESULT: The feeling of nasal obstruct were disappeared in 3 months after the surgery. The nasal airway resistance(NAR) of the ill nasal cavity was markedly decreased after the surgery, and the total NAR were decreasing unmarkably. The mucous proliferation was notable only in the first year after surgery, and especially on remains of the inferior turbinate or extra wall of nasal cavity. CONCLUSION: Lateral rhinotomy operation and medial maxillectomy have less impact on nasal airway and the functions.

Adult↗

PubMed2001

[Influence of anisodamine on guinea pig experimental nasal hypersensitivity].

OBJECTIVE: To observe the influence of anisodamine on guinea pig experimental nasal hypersensitivity. METHOD: Experimental animals were divided into normal control group, TDI group, reserpine group and anisodamine group. The scores of symptom of nose and the cytologic changes of nasal mucosa were parameters of observing. RESULT: The nasal symptom of reserpine group was significantly stronger than the TDI group, and the eosinophilia evidently increased (both P < 0.01). The nasal symptom of the anisodamine group reduced and the eosinophilia decreased. It is significantly difference between the TDI group and the reserpine group (both P < 0.01). CONCLUSION: Anisodamine may play a role in therapy of the experimental nasal hypersensitivity with hyperfunction of parasympathetic nerve.

Animals↗

PubMed2001

[Expression and the role of vascular endothelial growth factor mRNA in nasal polyps].

OBJECTIVE: To explore the pathogenesis of nasal polyps, the aim of this study is to detect the expression of vascular endothelial growth factor (VEGF) in nasal polyp(NP) tissue, and compare these findings with normal nasal turbinate. METHOD: We examined the expression of VEGF in human nasal polyps from patients underwent nasal polypectomy (n = 7) and nasal turbinate from patients with snoring (n = 6). VEGF mRNA expression was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). RESULT: VEGF mRNA expression was significantly stronger in the eight NP than in the turbinate tissue group (P < 0.05). CONCLUSION: We conclude that VEGF expression is upregulated in NP, which suggests a potential role of VEGF in the formation and growth and recrudescence of NP.

Adult↗

PubMed2001

[Cloning and DNA sequencing of Bacillus pumilus endo-1,4-beta-glucanase gene].

Bacillus pumilus S-27 screened from marine environment produces an extracellular endo-1,4-beta-glucanase. The enzyme has its optimal activity at pH 5.0-7.0 and 55 degrees C and still retained 79.0% of it at pH 9.0. The gene encoded this endo-1,4-beta-glucanase was cloned and sequenced. The structural gene contained an open reading frame of 1980 bp, corresponding to 660 amino acids, the amino acid sequence of this enzyme is very close to that of an EG of C. cellulovorans and an EG of Bacillus sp. KSM-522, all belong to the cellulase family E2.

Amino Acid Sequence↗

PubMed2001

[Purification and properties of chitinase from Enterobacter aerogenes].

A bacterium producing chitinase was isolated from the dead body of Gymephorap ruoergensis. A chitinase was isolated from the culture of E. aerogenes and purified by means of ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-100 column gel filtration. The purified chitinase showed homogeneity on the native polyacrylamide gel electrophoresis. Its molecular weight was estimated to be about 42.5 kD by SDS-PAGE. The optimum pH and temperature for hydrolysis of chitin were 6.0 and 55 degrees C respectively. Michaelis constant was 2.88 mg/mL. Different metal ions showed different effects on the chitinase activity, The chitinase activity was enhanced by Zn2+, Ba2+, Ca2+, Mn2+ and was strongly inhibited by Hg2+, Co2+, Mg2+.

Animals↗

PubMed2001

[N-terminal analysis and antibody preparation of insecticidal protein form Pseudomonas pseudoalcaligenes].

The insecticidal protein from Pseudomonas pseudoaligenes was and exotoxin which had toxicity on locusts. In order to elucidate its molecular properties an amino acid sequence, the insecticidal protein was purified from the culture supernatant by ultrafiltration, ion-exchange chromatography and gel filtration, and showed a single band on SDS-PAGE. Analysis of the purified insecticidal protein dentified N-terminal sequence of ten amino acid residues. Its polyclonal antibody was also obtained by immunizing rabbit with the insecticidal protein recovered form SDS-PAGE gel. The antibody titer determined by ELISA method was 1:12,800, indicating that it has high reactivity. Western blot analysis revealed that the antibody was spectific to 26 kD insecticidal protein, and did not cross-react with other proteins produced by the bacterium, suggesting that a specific antibody with high titer was obtained and could be used for further investigations of the gene cloning and expression of insecticidal protein.

Amino Acid Sequence↗

PubMed2001

[Clinical study on effect of zhuhuang granule no. 2 in treating psoriasis with liver-qi stagnancy].

OBJECTIVE: To observe the efficacy of Zhuhuang Granule No. 2 (ZHG2) in treating psoriasis with Liver-Qi stagnancy. METHODS: Sixty-seven patients were randomly divided into two groups, the 33 patients in the control group were treated with composite indigo capsule and the 34 in the treated group treated with ZHG2, and the clinical effect, changes of main symptoms and local skin lesion after treatment were evaluated. Meanwhile, the plasma levels of substance P (SP) and vasoactive intestinal peptide (VIP) in 15 patients and 13 healthy subjects were measured using radioimmunoassay (RIA). RESULTS: The total effective rate in the treated group and the control group was 97.1% and 78.8% respectively with significant difference, P < 0.05. ZHG2 showed a significant effect in reducing plasma level of SP and VIP in patients of psoriasis with Liver-Qi stagnancy. CONCLUSION: The effect of ZHG2 in treating psoriasis patients with Liver-Qi stagnancy is satisfactory, and worth further studying.

Adult↗

PubMed2001

[5,6-diaryl-2,3-dihydro-1-pyrrolizinone derivatives synthesis and antiiflammatory and analgesic activities].

AIM: To search for more potent and less toxic antiinflammatory and analgesic activity compounds. METHODS: A series of 5,6-diaryl-2,3-dihydro-1-pyrrolizinone derivatives were designed and synthesized based on the structures of diarylheterocyclic COX-2 selective inhibitors. Their structures were determined on the basis of spectal data (IR, MS and 1HNMR). Their antiinflammatory and analgesic activities in vivo were tested by xylene-induced mouse ear edema model and acetic acid-induced mouse writhing model p.o. dose of 200 mg.kg-1. RESULTS: Seventeen new compounds (1-17) were synthesized. Many of these compounds showed antiinflammatory and analgesic activities. CONCLUSION: Compound 3, 8, 11, 14 and 15 showed antiinflammatory activities more potent than ibuprofen. Compound 9, 10 and 11 showed analgesic activities comparable to ibuprofen. These compounds are regarded to be promising to develop new potent drugs.

Animals↗

PubMed2001

[The effects of safflor yellow on microcirculation and hemorheology of acute blood stasis rats].

OBJECTIVE: To observe the effects of safflor yellow on microcirculation and hemorheology of acute blood stasis rats. METHODS: The acute blood stasis medel was made by i.p. adrenalin (0.07 ml/(kg.time) for 2 times and the stimulation of ice-water. RESULTS: Safflor could markedly improve the number of net dot in microcirculation, and eta b, eta p, eta f in hemorheology (P < 0.05). CONCLUSION: Safflor yellow played an important role on blood stasis of rats.

Animals↗

PubMed2001

[The influence of type I collagen on the cell behavior of human embryonic periosteous osteoblasts].

OBJECTIVE: To study the influence of type I collagen (COL I) on the cell behavior of human periosteous osteoblasts (OB) and the application of type I collagen in constructing bioactive artifical bone. METHODS: OB were cultured on dishes coated with bovine type I collagen in different final concentrations. The cell adhesion was examined by the methods of cell count, the proliferation of OB was studied by 3H-TdR, and the osteoblastic ability was assessed by the synthesis of collagen, osteocalcin and alkaline phosphatase (ALP). RESULTS: OB cultured on type I collagen layer had the following characteristics: 1. The amounts of adhesive cells were maximal top in 25 micrograms/ml final concentration; 2. The proliferation of OB was decreased above 12.5 micrograms/ml final concentration (P < 0.05); 3. The synthesis of type I collagen was reduced slightly (above 25 micrograms/ml, P < 0.05); 4. The secretion of osteocalcin was increased markedly (above 6.25 micrograms/ml, P < 0.05, which reached maximally in 25 micrograms/ml); 5. The ALP activity was also increased (above 12.5 micrograms/ml, P < 0.05). CONCLUSION: Type I collagen promotes the expression of osteoblastic phenotype and cell adhesion. When the scaffold materials for bone tissue engineering are coated with type I collagen, the osteogenesis of OB is enhanced to accelerate the transformation course from artificial bone to biological bone, the best final concentration is 25 micrograms/ml.

Alkaline Phosphatase↗

PubMed2001

[Clinical application and evaluation of polymerase chain reaction-microwell plate hybridization assay in the detection of Mycobacterium tuberculosis].

OBJECTIVE: To evaluate the clinical application and feasibility of polymerase chain reaction-microwell plate hybridization assay in the detection of Mycobacterium tuberculosis. METHODS: 1130 specimens with strong suspicion for mycobacterium tuberculosis were collected from the hospitals and were detected by fast bacilli stain, culture, PCR-electrophoresis and PCR-microwell plate hybridization respectively. The laboratory results were analyzed in combination with the symptoms and signs of patients and the observations on treatment. Also detected were 100 samples from the clinically evidenced non-tuberculosis patients. RESULTS: In the 100 samples collected from the patients without tuberculosis, the PCR-hybridization method and culture method did not detect Mycobacterium tuberculosis, but the fast bacilli stain method and PCR-electrophoresis method brought out one and two false-positive results respectively. The results of testing the 1030 clinical samples which probably contained Mycobacterium tuberculosis demonstrated that the PCR-hybridization method had the highest positive rate (481/1030) among the four methods, and the positive rates of the other three methods were PCR-electrophoresis (406/1030), culture (365/1030) and fast bacilli stain (256/1030) in proper order. The chi-square test showed that there were significant difference between the PCR-hybridization method and the other three methods respectively (P < 0.0083 or P < 0.0017). CONCLUSION: PCR-Hybridization method is specific, sensitive, accurate and fast in detecting mycobacterium tuberculosis; it is worthy to be used clinically.

Evaluation Studies as Topic↗

PubMed2001

[An association study of histocompatibility leukocyte antigen-class II with Meniere's disease].

OBJECTIVE: To investigate the relation between histocompatibility leukocyte antigen (HLA)-class II DRB1 and Meniére's disease in Chinese. METHODS: Polymerase chain reaction-sequence specific primers (PCR-SSP) technique was used. HLA-class II allele distribution were measured in 60 patients with Meniére's disease, in comparison with those in 85 normal population. RESULTS: The results showed that the frequency of DRB1*09 allele was significantly lower in Meniére's disease patients than in those of controls (relative risk = 0.17, P < 0.01). CONCLUSION: HLA-DRB1*09 of the patients with Meniére's disease was significant low. It implied that DRB1*09 may be a protective gene for Meniére's disease.

Adolescent↗

PubMed2001

[Empty nose syndrome].

OBJECTIVE: The aim of this article is to present the concept of empty nose syndrome(ENS) and help ENT doctors take care of regular nasal turbinate surgery. METHODS: Fourteen patients who was diagnosed as ENS in our department were reviewed retrospectively. All patients had undergone various forms of nasal surgery (all had had turbinectomy). Their age ranged from 13 to 52 years. All patients were treated conservatively, among these patients 5 subjects who had more serious symptoms received submucous and subperiostal nasal implantation with ilium. RESULTS: All patients had nasal obstruction and dryness of nasal cavity, nasopharynx and oto-pharynx in 6 months--5 years after their first nasal surgery, some presented symptoms of depression. Nasoscope examination showed all patients had a cylindrically enlarged nasal cavity. Conservative treatment was effective in most cases. The effectiveness of operative treatment was encouraging during short-term follow-up. CONCLUSION: Extensive turbinectomy may cause secondary nasal mucosal atrophy and a series of subsequent symptoms. The presentation of this concept is to remind the ENT doctors prudently performing turbinectomy to avoid the occurrence of irreversible injury to the nasal cavity.

Adolescent↗

PubMed2001

[Expression of the inducible isoform of nitric oxide synthase mRNA and the role in nasal polyps].

OBJECTIVE: To explore the pathogenesis of nasal polyps. The aim of this study is to detect the expression of inducible isoform of nitric oxide synthase (iNOS) in nasal polyp tissue, and to compare these findings with that in normal nasal turbinate. METHODS: We examined the expression of iNOS in human nasal polyps from patients undergoing nasal polypectomy (n = 8) and nasal turbinectomy(n = 6). The iNOS mRNA expression was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The iNOS mRNA expression was significantly stronger in the nasal polyps (NP) than that in turbinate tissues (P < 0.01). CONCLUSIONS: iNOS expression is upregulated in NP, which indicate that iNOS and NO may play a potential role in the formation and growth of NP.

Adult↗

PubMed2001

[Study on calcium-cholate-phosphate ternary complexes by FTIR spectroscopy].

The aim of this paper was to examine the calcium-cholate-phosphate tenary complexes by FTIR spectroscopy. The results indicated that the mole ratio of cholate to phosphate were 1:3 and 1:4 in ternary complexes respectively when the initial mole ratio of cholate to phosphate were 3:1 and 1.5:1 in reaction. The FTIR spectroscopic results demonstrated that in the ternary complexes Ca2+ was coordinated with O in both COO- and PO(4)3- groups.

Calcium↗

PubMed2001

[Short-term versus long-term antimicrobial prophylaxis in abdominal surgery: a multicenter open randomized comparative trial].

OBJECTIVE: To evaluate the outcome of short-term antimicrobial prophylaxis versus long-term in surgical site infection (SSI). METHODS: 731 patients undergoing abdominal operation from 15 hospitals were randomly divided into 2 groups. 358 patients were enrolled in the short-term group (24 h), and 373 patients in the long-term group (72 h). There was no difference between the 2 groups with regard to age, sex, and types operation. Netilmicin was used alone or in combination with metronidazole. RESULTS: The rates of SSI in the short-term and long-term groups were 0.84% and 2.68% (P > 0.05), respectively. CONCLUSIONS: The results demonstrated that the short-term antimicrobial agents prophylaxis is effective in the prevention of post-operative SSI. The long-term one act does not better than the short-term in terms of rationality and pharmacoeconomics.

Abdomen↗

PubMed2000

Molecular and morphological evidence on the phylogeny of the Elephantidae.

The African and Asian elephants and the mammoth diverged ca. 4-6 million years ago and their phylogenetic relationship has been controversial. Morphological studies have suggested a mammoth Asian elephant relationship, while molecular studies have produced conflicting results. We obtained cytochrome b sequences of up to 545 base pairs from five mammoths, 14 Asian and eight African elephants. A high degree of polymorphism is detected within species. With a dugong sequence used as the outgroup, parsimony and maximum-likelihood analyses support a mammoth African elephant clade. As the dugong is a very distant outgroup, we employ likelihood analysis to root the tree with a molecular clock, and use bootstrap and Bayesian analyses to quantify the relative support for different topologies. The analyses support the mammoth African elephant relationship, although other trees cannot be rejected. Ancestral polymorphisms may have resulted in gene trees differing from the species phylogeny Examination of morphological data, especially from primitive fossil members, indicates that some supposed synapomorphies between the mammoth and Asian elephant are variable, others convergent or autapomorphous. A mammoth African elephant relationship is not excluded. Our results highlight the need, in both morphological and molecular phylogenetics, for multiple markers and close attention to within-taxon variation and outgroup selection.

Animals↗