Biomedical subjects

Z Yang

Publications and source records attributed to Z Yang.

At least 181 records · Page 10Linked to original sources

PubMed2001

Apolipoprotein E polymorphism in the early onset of coronary heart disease.

OBJECTIVE: To assess the relationship between apolipoprotein E (apoE) polymorphism and the early onset of coronary heart disease (CHD) and the effect of apoE on lipids and lipoproteins in healthy Chinese subjects. METHODS: Sixty-eight patients with CHD younger than 55 years (CHD1), 136 patients with CHD older than 65 years (CHD2), and 136 healthy subjects were enrolled, and their plasma levels of triglyceride (TG), total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) were determined. The apoE genotypes were identified by polymerase chain reaction-restriction fragment length polymorphism. RESULTS: apoE 3/4 genotype and E4 allele frequency in the CHD1 group were higher than those in the CHD2 group and healthy subjects, while no differences were found between CHD2 and healthy subjects. Meanwhile, the plasma levels of TC and low density lipoprotein cholesterol (LDL-C) were higher in the CHD2 group than in both CHD1 group and healthy subjects. Each apoE isoprotein has variable TC and LDL-C levels that is E2 (E2/2 + E2/3) < E3(E3/3) < E4(E4/4 + E3/4). CONCLUSION: apoE is one of the genetic factors that affect TC and LDL-C levels, and apoE 4 has a very close relation to CHD, suggesting that apoE 4 is an independent genetic factor of the early onset of CHD.

Adult↗

PubMed2001

[Role of vascular endothelial growth factor in the genesis of pregnancy induced hypertension and its relationship with nitric oxide].

OBJECTIVE: To study the role of vascular endothelial growth factor in the genesis of pregnancy induced hypertension (PIH) and its relationship with nitric oxide. METHODS: Serum vascular endothelial growth factor(VEGF) levels in 41 women with PIH and 20 normal pregnant women were measured by enzyme linked immunosorbent assay. The concentrations of nitric oxide (NO) in placental tissues were assayed by nitrite reductase. RESULTS: (1) Serum levels of vascular endothelial growth factor VEGF in PIH group were significantly lower than those in normal pregnancy. There was no difference between the mild PIH and normal group in the concentrations of VEGF. VEGF levels in moderate (23.1 +/- 4.1) ng/L and severe PIH (14.8 +/- 3.9) ng/L were significantly lower than those in normal group (P < 0.01). (2) The concentrations of NO in placental tissues in PIH group were significantly lower than those in normal pregnancy (P < 0.01). There was no difference between the concentrations of NO in mild PIH and those in normal pregnancy (P > 0.05). NO levels in moderate PIH (9.1 +/- 2.1) mumol/g and severe PIH (5.6 +/- 1.8) mumol/g were significantly lower than those in control group. (3) The serum levels of VEGF positively correlated with the concentration of NO in placental tissues. (r = 0.65, P < 0.01). CONCLUSION: The serum levels of VEGF and the concentrations of NO in women with PIH are both suppressed. The changes of VEGF levels might play an important role in the pathogenesis of PIH.

Endothelial Growth Factors↗

PubMed2001

[Comparison of the diagnostic value of telomerase activity with CEA level in benign and malignant pleural effusions].

OBJECTIVE: To study the diagnostic value of telomerase activity in differentiating malignant from nonmalignant pleural effusions. METHOD: Sixty-five specimens of pleural effusion were examined for telomerase activity and CEA level by PCR-ELISA and EIA, respectively. According to the final diagnosis achieved, the patients were classified into two groups: group I, 35 nonmalignant pleural effusions, group II, 30 malignant pleural effusions diagnosed by either fluid cytology or pleural biopsy. The results of telomerase activity were compared with CEA levels. RESULTS: Two of thirty-five (5.7%) samples of pleural effusions in group I were found to possess weak telomerase activity and 27 of 30 (90%) samples in group II showed positive enzymatic activity. The diagnostic sensitivity is 0.90, specificity is 0.94, and the positive and negative predictive value are 0.93 and 0.92, respectively. The overall accuracy is 0.92. The diagnostic efficiency of CEA as following: sensitivity 0.60, specificity 0.89, positive and negative predictive value are 0.82 and 0.72, respectively, accuracy 0.75. CONCLUSION: The results indicate that the detection of telomerase activity in pleural effusions is more sensitive, more accurate than CEA in differentiating benign from malignant effusions and can be used as an adjunctive measure in the diagnosis of malignant pleural effusions.

Adult↗

PubMed2001

[The treatment of osteoporosis and bone resorption of alveoli with alendronate in rat model].

OBJECTIVE: To evaluate the anti-osteoporosis effect of alendronate on bone in rat model. METHODS: The osteoporosis and alveolar bone resorption animal model were established in rats. The experimental groups were given alendronate in 5 mg/kg BW by subcutaneous injection after first day of ovariectomy and dental ligature, three times a week for 6 weeks. All the animals were sacrifised 12 weeks after the operation. The blood samples were collected for determination of biochemical indices. The left femora and jaw bones were processed for histomorphometry. The right femora and mendibles were prepared for determination of bone density and bone biodynamics. RESULTS: As compared with the control groups(without alendronate), the values of experimental groups (with alendronate) were all significantly improved on bone density, bone anti-flexure ability and body weight. The alkaline phosphatase, calcium in serum of ovariectomy groups(without alendronate) were obviously increased. In the experimental group, however, these indices were close to normal levels. In histomorphometry, there were little inflammation in gingiva and no obvious bone resorption in the alveolar crests of the experimental groups; but there were obvious gingivitis and alveolar crests resorption of the control groups. CONCLUSIONS: Alendronate can effectively prevent bone loss to reduce osteoporosis in ovariectomized rats and prohibit pathological alveolar bone resorption.

Alendronate↗

PubMed2001

[Pharmacokinetical study of safflor yellow on rat acute model of blood stasis syndrome].

OBJECTIVE: To study the pharmacokinetical character of safflor yellow in rat acute model of blood stasis syndrome. METHODS: The rat acute model of blood stasis syndrome was made by i.p. adrenalin (0.07 ml/kg) and the stimulation of ice-water. At the time of 5, 10, 30, 60, 120, 180, 320, 480 min, the concentration of safflor yellow in the serum after i.v. safflor yellow (37.08 ml/kg) was determined. RESULTS: The concentration-time date of safflor yellow in rat acute model of blood stasis syndrome was shown to fit a double-compartment open model, t1/2 alpha = 1.43 min, t1/2 beta = 95.65 min, AUC = 49,632.90 micrograms.min/ml; while the concentration-time data of safflor yellow in normal rat was shown to fit a single-compartment open model, t1/2 alpha = 66.27 min, AUC = 42,267 micrograms.min/ml. CONCLUSION: The metabolism of safflor yellow was evidently later in rat acute model of blood stasis syndrome than in normal rat.

Acute Disease↗

PubMed2001

Regulative effect of P38MAPK on release of TNFalpha and NO from alveolar macrophages under endotoxin stimulation.

OBJECTIVE: To evaluate activation of P38 mitogen-activated protein kinase (P38MAPK) in alveolar macrophage (AM), release of TNFalpha and NO from cells, and their relationship following lipopolysaccharide (LPS) stimulation. METHODS: AM was isolated from branch alveolar lavage fluid (BALF). The activation of P38MAPK was assayed by Western blot. SB203580, a specific inhibitor of P38MAPK, was used with gradient concentration to evaluate the regulative effect of P38MAPK on the release of TNFalpha and NO from AM. RESULTS: P38MAPK was activated by LPS (100 ng/ml) with peak activation at 30 minutes. The activation of P38MAPK was inhibited by SB203580. The secretion of TNFalpha and NO stimulated with LPS increased (P<0.01) and was inhibited by SB203580 significantly. CONCLUSIONS: The results indicate that P38MAPK is involved in the secreting process of TNFalpha and NO following LPS stimulation. P38MAPK may be an important site for controlling the secretion of both inflammatory mediators during lung inflammatory disorders.

Animals↗

PubMed2001

Signal transduction pathway of nitric oxide inducing PC12 cell death.

OBJECTIVE: To study signal transduction pathway of nitric oxide inducing death of PC12 cells. METHODS: Cell survival rate was measured with MTT assay, and caspase-3 activity with caspase-3 assay kits after PC12 cells were incubated with sodium nitroprusside (SNP), caspase-3 inhibitor II plus SNP or p38 inhibitor-SB203580 plus SNP. RESULTS: SNP induced death of PC12 cells in dose- and time-dependent manner and enhanced caspase-3 activity gradually. Both caspase-3 inhibitor II and SB203580 reduced cell death, but SB203580 reduced caspase-3 activity significantly. CONCLUSIONS: NO may induce death of PC12 cells through activation of p38 and caspase-3.

Animals↗

PubMed2001

[An experimental study of the effects of thrombin receptor activating peptide (TP508) on healing of ischemic wound and flap survival in rats].

OBJECTIVE: To investigate the effects of the thrombin receptor activating peptide (TP508) on healing of ischemic wound and flap survival in rats. METHODS: Sixty-six Sprague-Dawley rats were employed as the model. On the back of the rats, three kinds of wound and flap were made to establish four groups as follows: partial ischemic wound in 16, full ischemic wound in 16, routine wound in 18 and flap in 16 rats. Each group was further divided into TP508 treating group and isotonic saline control group. The total and necrotic areas of the wounds and flaps were daplicated on acetate papers and calculated with a computer on the 3rd, 7th, 10th and 14th post-operation days (PODs). RESULTS: In routine wounds, the ischemic wound area treated by TP508 was 73.7% and 45.4% in saline control groups on 7 and 14 (PODs), respectively. While in the flap model, the necrotic flap area treated by TP508 was 80.4% and 56.8% in control groups on 7 and 14 (PODs), respectively. CONCLUSION: TP508 could accelerate healing of ischemic wound and improve flap survival in rats.

Animals↗

PubMed2001

[Current status and distribution of deafness in the elderly in several cities in China].

OBJECTIVE: To survey the current status and distribution of deafness and its effect on daily life activities in the old population. METHODS: Eight thousand two hundred and fifty-two elderly aged 60 years and above in the urban and rural areas of Beijing, Shanghai, Guangzhou, Chengdu, Xi'an and Shenyang were investigated, using a clustered random sampling methods. RESULTS: Overall crude prevalence of deafness in the elderly was found to be 33.7%, but the self-reported crude prevalence was low, only 47.1% when shown by medical examination. Crude prevalence rates were increasing with ageing, with 21.6%, 30.0%, 35.6%, 42.6%, 55.5% and 61.6% respectively (P < 0.01) in the age groups of 60-, 65-, 70-, 75-, 80-, 85- years old. Rate of deafness was highest in Beijing (58.5%), among the in-house workers (48.9%) and the lowest in scientists, teacher and health workers (28.5%). Logistic regression analysis showed that the crude prevalence was related to ageing, profession and area (P respective < 0.01). CONCLUSION: The prevalence of deafness was high in the elderly which increased with ageing with different areas, professions and the level of education. It was lower when self-reported than shown by medical examination. Prevention and treatment of deafness in the elderly should be strengthened.

Age Factors↗

PubMed2001

[The scleral buckling combined with argon laser photocoagulation for retinal detachment].

OBJECTIVE: To investigate the effects and indications of postoperative photocoagulation with argon laser instead of intraoperative cryotherapy for closing the retinal breaks during the scleral buckling operation in rhegmatogenous retinal detachment (RD) METHODS: An analysis of clinical data in 35 RD cases (38 eyes) was conducted retrospectively. These cases were treated by argon laser photocoagulation after the scleral buckling surgery from December, 1999 to April, 2000 in our hospital. RESULTS: Thirty-eight eyes were followed-up from 3 to 6 months. In 36 of 38 eyes, the retina was reattached after surgery (94.7%). Postoperatively, thirty-one eyes (86. 1%) had a visual acuity (VA) of >or= 0.05 and the best VA was 1.0. CONCLUSIONS: The method using postoperative photocoagulation instead of cryotherapy during scleral buckling surgery can simplify the surgical manipulations, alleviate the vessel damage of the retina and uvea and reduce the postoperative complications. It is a practicable way to treat RD. Surely, photocoagulation can not totally replace cryotherapy. Proper selection of indications and avoidance of complications of photocoagulation can elevate the successful rate of reattachment of RD, and promote the recovery of visual function.

Adolescent↗

PubMed2001

[The relationship of clinicopathology and immunophenotypes in non-Hodgkin's lymphomas of the spleen].

OBJECTIVE: To investigate the relationship between clinicopathologic features and immunophenotypes in non-Hodgkin's lymphoma (NHL) of the spleen. METHODS: Clinicopathologic analysis and follow up; immunohistologic staining by SP method and EBER in situ hybridization. RESULTS: (1) Splenomegaly was found in all 19 cases of spleen NHL, 10 of the 19 (52.6%) cases had masses or a mass in their enlarged spleen; (2) 14 of the 19 (73.7%) cases were of B-cell type (including 5 cases of follicular type and 9 cases of diffuse type), in which 8 cases were centroblastic, 3 of centroblast/centrocyte and 4 cases of small lymphocyte types; all 10 cases of primary splenic lymphomas (PSL) were B-cell type; (3) 5 of the 19 cases (26.3%) had peripheral T-cell origin, including 4 cases of large cell type and 1 case of small cell type; of the 3 TIA-1 positive cases, 2 were also positive for EBER 1/2 (one CD 8+ and another CD56+), the remaining case was CD8-, CD56-, EBER1/2-; all 5 cases were secondary NHL of the spleen; (4) Follow up data were obtained for 14/19 cases (73.7%). 8 of 9 surviving cases were primary NHL. The range of survival period varied from 8 to 10 years. All 5 deceased cases had secondary NHL and their survival period was 2 to 6 months. CONCLUSIONS: Some relationship exists between the clinicopathology and origin of the tumor cells in splenic NHL, the prognosis of primary NHL is better than that of the secondary NHL and the diagnosis of primary NHL should be made with discretion.

Adolescent↗

PubMed2001

[Detection and analysis of SYT-SSX fusion gene in synovial sarcoma].

OBJECTIVE: To demonstrate that a characteristic SYT-SSX fusion gene resulting from chromosomal translocation t (X; 18) (p11; q11) was detectable in archival paraffin-embedded synovial sarcoma tissues. METHODS: SYT-SSX fusion transcripts in 20 synovial sarcomas (histologic subtypes, 15 monophasic and 5 biphasic) were detected and analyzed by the reverse-transcriptase polymerase chain reaction and compared with the results from relevant pathological data. RESULTS: A specific SYT-SSX RT-PCR product was found in 19 of 20 (95%) synovial sarcomas tested and of the 13 tumors containing SYT-SSX2, 10 were monophasic. CONCLUSION: SYT-SSX fusion transcripts are considered as a defining diagnostic marker of synovial sarcomas and the subtypes of SYT-SSX fusion transcripts (SYT-SSX1 and SYT-SSX2) may yield prognostic information.

Adolescent↗

PubMed2001

[An experimental study on the apoptosis of PMNs and macrophages during the early postburn stage in severely scalded rats].

OBJECTIVE: To investigate the changes of postburn apoptosis of PMN and the apoptosis of PMN and macrophages after being stimulated by burn sera and the change of the phagocytosis of apoptotic PMNs by macrophages. METHODS: Rats inflicted by 30% TBSA III degree scalding were employed as the model. PMNs were harvested on the 0, 6, 12 and 24 postburn hour (PBH). Burn sera were harvested at 24 PBHs. PMN apoptosis was detected by flow cytometry. Simultaneously, peritoneal macrophages were collected for the study. The apoptotic change of the macrophages (Mphis) after being stimulated by burn sera and the ability of Mphis phagocytizing apoptotic PMNs at 24 PBHs were examined. RESULTS: The PMN apoptosis was obviously inhibited at 6, 12 and 24 PBHs in the scalded rats with no difference between the time spots. Twenty-four hours after being stimulated by burn sera, the apoptotic rate of PMNs on scalded rats was lower than that in normal rats, while the apoptotic rate of Mphis in scalded rats was higher than that in normal rats. The ability of Mphis phagocytizing apoptotic PMNs decreased after severe burn (P < 0.01). CONCLUSION: There exhibited delayed rat PMN apoptosis and decreased ability of the phagocytosis of apoptotic PMNs by the Mphis after severe burn or being stimulated by burn sera. This might lead to the incomplete phagocytosis of apoptotic PMNs by Mphis. As a result, the PMNs would finally come to necrosis and release toxic contents which were important factors in the inducing of SIRS.

Animals↗

PubMed2001

[Investigation on method speliality in identifying Fructus Crataegi with vitexin rhamnoside].

The methanol extract of Fructus Crataegi was purified on macro-reticular resins(model DM130)-polyamide column. Vitexin rhamnoside(I) was identified from Fructus Crataegi by TLC on polyamide layer sheets. The false and the confused species of Fructus Crataegi and some traditional Chinese herbal medicines which contain vitexin were studied by the same method. The results indicated that compound I was special constitute of Fructus Crataegi and the identification method of Fructus Crataegi with I was special.

Apigenin↗

PubMed2001

[Amphotericin B for treatment of fungal infections in 40 patients with malignant hematologic diseases].

OBJECTIVE: To observe the therapeutic effect and side effects of amphotericin B for fungal infections in patients with malignant hematologic diseases. METHODS: 40 patients (male 27, female 13; average age 35.5 years) with malignant hematologic diseases were given amphotericin B, 5-50 mg/d per day for 5-85 days (average time 21 days). RESULTS: The clinical efficacy rate of amphotericin B was 52.5%, and the fungal elimination rate was 56.2%. Among the side effects, rigor and fever were present in 2.5% of the patients. Hypokalaemia was found in 12.5%, hepatotoxicity in 15.0% and nephrotoxicity in 15.0%. CONCLUSION: As amphotericin B has a broad anti-fungal spectrum and relatively good efficacy, it is still a high-efficiency drug in treatment of systematical fungal infections. However, the use of drug is limited because of its many side effects. Our study indicates that if it is used properly and hepatic and renal function tests are carried out regularly, amphotericin B is a relatively safe and effective drug.

Adolescent↗

PubMed2001

[Anti-virus effect of combination of attenuated measles virus with 3TC on duck hepatitis B model].

OBJECTIVE: To evaluate the anti-virus effect of combination of attenuated measles virus with 3TC on duck hepatitis B model. METHODS: Guanzhou brown spot ducklings infected with DHBV were divided into three groups. Drug treatments including measles vaccine, 3TC and both measles vaccine and 3TC were given in the 13th day after the infection, respectively. Serum samples were obtained from all ducklings before, during and after treatment at different times and stored at -70 degrees C. DHBV DNA was detected by Dot-blot hybridization. RESULTS: Compared with the levels of DHBV DNA before and after treatment, no statistic difference was found in group of 3TC alone. The levels of DHBV DNA were not changed in group of measles vaccine alone in early stage, but decrease of DHBV DNA occurred after stopping treatment (comparison of the levels T13 with before treatment and T13 of positive group, P < 0.01 and P < 0.05, respectively). DHBV DNA level in group of measles vaccine combined with 3TC was obviously lower at 5th day after the treatment than in both measles vaccine and 3TC alone groups at the same time(P < 0.05 and P < 0.01, respectively), and it remained low when the treatment was discontinued. CONCLUSION: Attenuated measles virus combined with 3TC may have early and long-lasting inhibiting effect on the replication of DHBV.

Animals↗

PubMed2001

[An experimental study on effects of pingyangmycin on vessels].

OBJECTIVE: The aim of this study was to investigate the mechanism of Pingyangmycin sclerotherapy for cavernous hemangiomas. METHODS: Totally 9 rabbits (one as the control) were selected to be injected with Pingyangmycin and sodium morrhuate into the auricularis posterior vein, and then these veins were examined histologically 2, 7, 14 and 21 days after injection respectively. RESULTS: After injection, Pingyangmycin nonspecifically made the endothelia and veins defective, and induced proliferation of endothelial cells and smooth muscle cells after 7 days. Further, the veins became sclerostenosis 21 days after injection. On the other hand, sodium morrhuate caused thrombosis quickly and the vascular cavity disappeared. Finally, the thrombus became fibrogenesis. The local skin could be observed swollen and necrosis. CONCLUSION: Pingyangmycin is a better sclerosant for the therapy of cavernous hemangioma.

Animals↗

PubMed2001

[Nasal cavity dimensions in the infants measured with acoustic rhinometry].

OBJECTIVE: To investigate the range of normal value of minimal cross-sectional area (MCA) of the nasal cavity, distance of minimal cross-sectional area from the nostril (DCA), total nasal cavity volume(NV) and total nasopharyngeal cavity volume (NPV) of healthy infants. METHOD: One hundred and seventy-five healthy infants and one hundred and eight infants suffered from acute upper respiratory tract infection were measured with acoustic rhinometry. RESULT: The range of total MCA of healthy infants was 0.2-0.5 (cm)2, DCA was 1.93-2.47 cm, NV was 2.69-4.75 cm3 and NPV was 3.83-8.92 cm3. Comparing healthy infants with infants suffered from acute upper respiratory tract infection, there all were significant differences in MCA, DCA and NV. CONCLUSION: Acoustic rhinometry suit objective assessment of the nasal airways in infants. It is demonstrated that these data can provide available information for the study of nasal physiology and pathophysiology, well as for the diagnosis and judgement of therapeutic effectiveness of nasal diseases.

Child, Preschool↗