Biomedical subjects

Z Wu

Publications and source records attributed to Z Wu.

At least 145 records · Page 8Linked to original sources

PubMed2001

[Recovery of zinc ion and cadmium ion with hollow fiber membrane extraction].

The efficiency of hollow fiber membrane solvent extraction for the system of Meaq/bis(2-ethylhexyl) phosphate in heptane(Me = Cd2+, Zn2+) was studied for wastewater treatment and hydrometallurgy. It was found that the two phases velocity, the initial concentration and the pH value of the aqueous phase greatly influenced the extraction ratio. For very dilute solution with concentration lower than 500 mg/L, the mass transfer resistance was mainly in aqueous phase. When the aqueous phase concentration was relative higher than that of dilute solution, all the individual mass transfer resistances would not be ignored. For the concentrated solution, the mass transfer in the organic phase and the diffusion in membrane pores controlled the transport process. In the back extraction, the mass transfer resistance lied in the organic phase and the diffusion in membrane pores. The experimental results showed that it was possible that the extraction percentage achieved 90% when the metal ion concentration was lower than 400 mg/L. When the cadmium concentration was lower than 200 mg/L, its concentration can decrease 2 orders by extraction in a single-pass flow mode. The values of (HTU)w were between 15 cm and 30 cm, which was much lower than those of traditional extraction columns. The results indicated that membrane extraction with hollow fiber modules could efficiently remove or recover metal ions in the aqueous phases.

Cadmium↗

PubMed2001

[Acceptability and feasibility of promoting condom use among families with human immunodeficiency virus infection in rural area of China].

OBJECTIVE: To study the acceptability and feasibility of promoting condom use among families with human immunodeficiency virus (HIV) infection in rural area of China. METHODS: Ninety families with HIV infection were selected. Education, skill training on condom use and distribution of condoms were carried out. Two cross-sectional surveys were carried out before and after intervention to compare the difference of condom use rate. RESULTS: Eighty-four families finished the study, with a follow-up rate of 93.3%. The rate of use condom for every sexual intercourse in the last month increased from 2.6% before intervention to 71.4% after intervention (P < 0.001). After controlling other confounders, intervention is a strong independent predictor for increased condom use in infected couples. CONCLUSION: Promoting condom use among families with HIV infection in the rural area is acceptable. To reduce risk of sexual transmission of HIV within couples, families of the HIV infected persons need to be informed in no time.

Adult↗

PubMed2001

[Mutation and polymorphism in exon 4 of tuberous sclerosis complex gene].

OBJECTIVE: To study the characteristic of mutation and polymorphism in exon 4 of tuberous sclerosis complex gene(TSC1) in Chinese. METHODS: Twenty-five TSC patients and 23 parents from 21 families were enrolled. The mutation of exon 4 in these subjects was identified by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and further confirmed by direct sequencing. RESULTS: The normal controls had the same SSCP bands. In 25 TSC patients, a sporadic case was found to display variant banding pattern and be heterozygous for 352 insA mutation by sequencing. In 23 parents who were normal on clinical examination, another bandshift was found on a mother who had two affected children, which was confirmed as 347A-->C(Val42Val) single nucleotide polymorphism(SNP) by sequencing. CONCLUSION: The 352 insA mutation is a new causative mutation and the 347A-->C is a rare single nucleotide polymorphism.

Adolescent↗

PubMed2001

Liposome-C-erbB2 antisense oligodoxynucleotides in human ovarian cancer cells.

OBJECTIVE: To explore the effects of liposome-C-erbB2 antisense phosphorothioate oligodeoxynucleotides (S-ODNs) on C-erbB2 proto-oncogene expression and cell proliferation in human ovarian cancer cells. METHODS: The effects of liposome-C-erbB2 S-ODNs on C-erbB2 protein expression, cell cycle and cell proliferation in human ovarian cancer cells were studied by means of flow cytometry and 3H-thymidine incorporation. RESULTS: Liposome-C-erbB2 S-ODNs can specifically reduce C-erbB2 protein expression in human ovarian cancer cells, accompanied by a 30% inhibition of cell proliferation. The effectiveness of liposome-C-erbB2 S-ODNs on the expression of C-erbB2 was about 40 times higher than that of C-erbB2 S-ODNs. CONCLUSIONS: The data suggest that antisense therapy might be a useful method of gene therapy in ovarian cancer. The effectiveness of C-erbB2 S-ODNs could be greatly increased by adsorption of S-ODNs by liposomes.

Cell Cycle↗

PubMed2001

Changes in intestinal microflora in patients with chronic severe hepatitis.

OBJECTIVE: To investigate changes in intestinal microflora in patients with chronic severe hepatitis (CSH), and their role in this life-threatening disease. METHODS: We classified nineteen patients with chronic severe hepatitis as the CSH group, thirty patients with chronic hepatitis (CH) as the CH group and thirty-one healthy volunteer as the control group. Fecal flora from all subjects were analyzed. Concentrations of plasma endotoxin, serum cytokines tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) and liver function were assessed. RESULTS: The number of fecal bifidobacterium (P < 0.001, P < 0.05 respectively), as well as bacteroidaceae (P < 0.001, P < 0.01 respectively) were significantly deceased in patients with chronic severe hepatitis compared with the CH and control groups, while the number of enterobacteriaceae (P < 0.001, P < 0.05 respectively) and yeasts (P < 0.01, P < 0.05 respectively) were significantly increased. Levels of plasma endotoxin, serum TNF-alpha, IL-1 beta and total bilirubin (TBiL) were significantly increased in the CSH group. The concentration of endotoxin positively correlated with levels of both TNF-alpha, IL-1 beta and TBiL (P < 0.001, respectively). Levels of plasma endotoxin were positively correlated with the number of fecal enterobacteriaceae and negatively correlated with bifidobacterium (P < 0.05, P < 0.001, respectively). CONCLUSION: Intestinal flora in patients with chronic severe hepatitis were severely disturbed and gut microbiological colonization resistance was impaired. Changes in intestinal flora may have a pivotal role in both the elevation of plasma endotoxin and further hepatic lesions resulting in liver failure.

Adult↗

PubMed2001

[Study on the intestinal microbial colonization resistance in patients with chronic severe hepatitis].

OBJECTIVE: To investigate the changes of microbial colonization resistance of the bowel in patients with chronic severe hepatitis. METHODS: The fecal flora bifidobacteria, enterobacteriaceae, enterococci, and yeasts of subjects (chronic severe hepatitis, chronic hepatitis, and healthy volunteers) were quantitatively and qualitatively analyzed by Mitsuoka's methods. While the ratio of bifidobacterium to enterobacteriaceae ( B/E ratio ) was calculated. RESULTS: The number of bifidobacteria and the B/E ratio in chronic severe hepatitis group was significantly deceased compared with that of the control group (P<0.001). The number of enterobacteriaceae and yeasts was significantly increased compared with that of control group (P<0.001 and P<0.01, respectively). CONCLUSIONS: The results suggest that the intestinal microbial colonization resistance in patients with chronic severe hepatitis is seriously impaired.

Bifidobacterium↗

PubMed2001

[Effect of hypertonic saline solution on the viscoelasticities of erythrocyte membrane in rats subjected to hemorrhagic shock].

We have studied the effect of hypertonic saline solution on the viscoelasticities of erythrocyte membrane in hemorrhage-shocked rats using micropippette aspiration technique. Wistar rats were randomly divided into three groups of 0.9% NaCl(NS), 7.5% NaCl (HS) and 5% NaCl-3.5% NaAc (HSA), respectively. The animals were bled to reach a mean arterial pressure of 5.3 kPa in 10 minutes and maintained in shock for 90 minutes. 4 ml/kg NS, HS and HSA was given intravenously and respectively in 5 minutes following hemorrhagic shock. The blood was collected to determine the viscoelasticities of erythrocyte membrane at baseline, shock and after treatment. The results showed that the elastic moduli and viscous coefficients of erythrocyte membrane were increased obviously following hemorrhagic shock. HS raised elastic moduli and reduced viscous coefficients significantly compared with NS after treatment. The elastic moduli and viscous coefficients of erythrocyte membrane were decreased remarkably in HSA group than in NS and HS group. These data suggested that HSA could improve the viscoelasticities of erythrocyte membrane significantly in rats subjected to hemorrhagic shock.

Animals↗

PubMed2001

[The achievement of industrialized healthy aquatic breeding of hippocampus and its application prospects].

The artificial industrialized aquatic breeding of Hippocampus, which is a kind of Chinese medicinal marine material, has been achieved in the artificial aquatic breeding base of Hippocampus of Guangdong Richvast Group Company since 1983. This article reported part of the achievements of the large-scale artificial aquatic breeding experiments. The prospects for the research and application of Hippocampus as health foods and drugs were also analyzed.

Animals↗

PubMed2001

[Treatment of malignant pleural effusion using vindesine and cisplatine intrapleurally injection (observation of 60 cases)].

OBJECTIVE: To evaluate the efficacy of treating malignant pleural effusion with cisplatine and vindesine by injection through thoracic tube intubation. METHODS: 60 cases with malignant pleural effusion, after drainage, vindesine 4 mg, cisplatine 40 mg/m(2) were injectred into pleural cavity in therapy group (T), and only cisplatine 40 mg/m(2) in control group (C). the tretment was repeated after a week, then evaluate the efficacy, survival rate, toxicities and quality of life. RESULTS: The response rate was 81% in T group and 50% in C group. There was statistical difference in two groups (P < 0.05). The Karnofsky score is higher in T group than in C group. The half-year, one-year, one and half-year, two-year survival rates were 97%, 59%, 44%, 6% in T group and 71%, 50%, 14%, 4% in C group. Grade I leukopenia is higher in T group than in C group. CONCLUSION: The treatment to use Vindesine and cisplatine through thoracic tube is regarded as effective, slight side-effect and well tolerated method for malignant pleural effusion.

Adolescent↗

PubMed2001

[A dynamic functional comparative analysis of screw retained vs cement retained crowns under cyclic fatigue testing].

OBJECTIVE: To investigate the stability and mechanical characteristics of screw-retained and cement-retained crowns under dynamic cyclic fatigue testing. METHODS: Screw-retained and cement-retained crown samples were fixed on a 227 kg pressure transducer, through computer control cyclic fatigue testing was performed under a load of 15 kg on the sample during testing. Crown mobility was assessed in PTV value and the failure pattern of crowns were recorded. RESULTS: The initial PTV of cement-retained and screw-retained samples were not significant difference. The stability of the two group samples was no significant difference after testing. However the pattern of failure in two group samples was different. CONCLUSION: Both screw-retained and cement-retained methods may provide sufficient retentive force to keep stability of the crown in masticatory function.

Biomechanical Phenomena↗

PubMed2001

Clinical retrospective and comparative study on diaphragm injuries in 46 cases.

OBJECTIVE: To explore a way of guiding diagnosis and treatment of blunt and penetrating diaphragm injuries. METHODS: According to injury violence, 46 chest trauma patients with diaphragm rupture were divided into two groups: a blunt injury group and a penetrating injury group. The injury condition and trauma scores between the two groups were compared and analyzed. RESULTS: The incidence of blunt diaphragm injuries was lower than that of penetrating injuries (1.78% vs 8.53%, P <0.05). In the blunt injury group most patients had multiple injuries. Penetrating injuries developed more quickly than blunt injuries, and resulted in hemorrhagic shock in the early period. Trauma scores showed that there was no significant difference in the Revised Trauma Score (RTS), the Injury Severity Score (ISS) and thoracic Abbreviated Injury Scale (AIS) between the two groups (P<0.05), but the blunt injury group had lower Glasgow Coma Scale (GCS) and abdominal AIS than the penetrating group (P<0.0 5). CONCLUSIONS: Blunt and penetrating diaphragm injuries have different clinical characteristics. So they should be dealt with differently to reduce the incidence of complication and improve prognosis.

Adolescent↗

PubMed2001

[A study on the epidemiological characteristics and the preventive measures of rabies in Shanghai].

OBJECTIVE: To analyze the epidemiological characteristics and the preventive measures of rabies in Shanghai from 1950 to 2000. METHODS: Suspected cases of rabies were investigated epidemiologically and collection of samples to isolate the virus. RESULTS: The most highest morbidity of rabies was 1.98/100,000 in 1950. There had been neither case of rabies, nor the rabies virus positive identified in dogs between 1958 and 1988. The morbidity of rabies was 0 - 0.03/100,000 from 1989 to 2000. According to the data of 20 reported cases from 1985 to Sept. 2000, the mean incubation was 66.37 days, except one case of 14 years. The mean course of diseases was 4.55 days. Seventy percent of the cases did not receive any preventive measure. A hundred and ninety-four persons who were bitten by rabies virus positive dogs were timely treated and all of them survived. In recent years, there has been more than 50,000 persons bitten by dogs every year in Shanghai. The rate of rabies vaccine immunization was over 95%. The rate of anti-rabies serum immunization was over 20%. CONCLUSION: Rabies is a preventable disease even when persons were bitten by dogs with rabies virus positive. The important measure is to treat the wound timely, giving vaccine and anti-rabies serum injection. According to the regulation of dog control in Shanghai, the number of dogs be kept at the lowest level, immunizing all the registration dogs, and destroying the none-registration dogs are the most essential measures to be taken.

Animals↗

PubMed2001

[Study of triglyceride distribution aged 35 - 64, and their association with other cardiovascular disease risk factors in 11 provinces].

OBJECTIVE: Study of triglyceride distribution and their association with other major cardiovascular disease risk factors in 11 provinces. METHODS: Twenty-seven thousand and sixteen persons in 11 provinces, aged 35 - 64, were screened for cardiovascular disease risk factors. RESULTS: (1) Results of plasma triglyceride level distribution showed that there was significant difference among 11 province, the highest/lowest ratio was 2.3. (2) Plasma triglyceride level was higher in men than in women (P < 0.001). (3) The prevelance of hypertriglyceridemia was the most frequent type of the dislipidemia. (4) Multiple stepwise regression analysis showed that total cholesterol, high-density lipoprotein cholesterol, body mass index, blood pressure, blood glucose and physical activity, significantly and independently correlated with hypertriglyceridemia prevelance. (5) There existed aggregation of risk factors in hypertriglyceridemia. Seventy-one point zero percent of hypertriglyceridemia had at least one other risk factors in men and 74.4% in women, while it was 53.8% men and 52.2% women of those with non hypertriglyceridemia (P < 0.01). CONCLUSION: Our date indicated that fasting triglycerides were associated with more arteriosclerosis factors and could serve as a marker for several cardiovascular disease risk factors. More patients with hypertriglyceridemia combined with other situation of metabolic syndromes thus might have higher risks.

Adult↗

PubMed2001

[Changes in Descemet's membrane and endothelium after photorefractive keratectomy in rabbits].

OBJECTIVE: To investigate the effects of epithelial abrasion on the corneal endothelium and Descemet's membrane and to compare the changes with those after eximer laser photoablation of the cornea. METHODS: The animal models of corneal epithelial abrasion and excimer laser photoablation of the cornea were performed on the rabbit eyes. The central epithelial abrasion, 7 mm in diameter, was created by mechanical removal of the epithelium, and the specimens were examined at 24 hours, 7 days, 14 days and 30 days by transmission and scanning electron microscopy. Corneas that were photoablated by an excimer laser and non-treated normal corneas were investigated as controls. RESULTS: (1) Corneas with the epithelium removal showed massive enlargement of the mitochondria in the endothelium and exhibited a layer of electron-dense fibrillogranular material that had migrated forward through the Descemet's membrane. These alterations were similar to the changes observed after photoablation of the cornea by an excimer laser. (2) The edema and pathologic changes of corneal endothelium in eyes with denuded epithelium alone were similar to that observed after photoablation of the cornea by an excimer laser. CONCLUSION: The extrusion of electron-dense material in the Descemet's membrane observed after excimer laser ablation destruction of the epithelial integrity.

Animals↗

PubMed2001

[A study on the detection of apoptosis of hypertrophic scar and its related modulating factors].

OBJECTIVE: To investigate the relationships between the development of hypertrophic scar (HS) and apoptosis of fibroblast and endothelial cell, and to elucidate the influence of related modulating factors. METHODS: TUNEL and immunohistochemistry staining techniques were employed to examine apoptosis of fibroblast and endothelial cell. The expression of ICE and Bcl-2 in hypertrophic scar of 61 cases of postburn patients undergoing plastic surgery with HS and in 20 other operative patients with non-hypertrophic scars (NHS) was also determined. RESULTS: The positive apoptotic cell indices of fibroblast and vascular endothelial cells during the proliferative stage of HS were 6.60 plus minus 4.43 and 8.90 plus minus 6.01 respectively, while those during the mature stage were 25.60 plus minus 5.70 and and 26.6 plus minus 6.02, respectively. There existed evident differences in positive apoptotic cell indices between the same kind of cells (fibroblasts or vascular endothelial cells) during the two stage (P < 0.01). The ICE positive expression rate in proliferative stage was much lower than that in mature stage (P < 0.01), but the Bcl-2 positive expression rate in proliferative stage was obviously higher than that in mature stage (P < 0.01). CONCLUSION: HS development might be related to the decrement of apoptotic fibroblasts and endothelial cells. ICE and Bcl-2 were possibly involved in the modulation of fibroblast and endothelial cell apoptosis in HS.

Adolescent↗

PubMed2001

[Studies on chemical constitutents from Penthorum chinense Pursh].

OBJECTIVE: To study the chemical constituents of Penthorum chinense. METHOD: Silica gel and macroporous resin were used as adsorbent for isolation and purification. The chemical structures were elucidated by spectral analysis and chemical methods. RESULT: Three compounds were isolated from the water-extract of P. chinense. The structures were identified as 5-hydroxy-flavanone-7-O-beta-D-glucoside (I), 2,4,6-trihydroxybenzoic acid (II) and quercetin(III), respectively. CONCLUSION: Compounds I and II were isolated from this plant for the first time.

Flavanones↗

PubMed2001

[Relation of plasma glucose levels to serum lipids and apolipoproteins levels in middle and old age males].

OBJECTIVE: To gain an insight into the relation of plasma glucose levels to lipids and apolipoproteins levels. METHODS: Fasting 12-14 h blood glucose (BG), high density lipoprotein cholestrol(LDL-C), apolipoproteins(apo) A I, A II, B100, C II, C III and E levels were measured in 735 men aged 40-70 in Chengdu area. The subjects studied were divided into three groups by the plasma glucose levels: 1. normal group (S1), 2. decreased glucose tolerance group (S2), 3. diabetes mellitus (DM) group (S3). RESULTS: Among 735 cases studied, groups S2 and S3 had more hyperlipidemia than group S1 (64%, 45% and 32.2% respectively). The fasting serum mean TG levels in groups S2 (3.62 +/- 2.52 mmol/L) and S3 (4.45 +/- 3.82 mmol/L) group were significantly higher than that in group S1 (1.92 +/- 1.39 mmol/L) (P < 0.01 and P < 0.01), and the mean TG levels in groups S2 (5.68 +/- 1.15 mmol/L) and S3 (5.68 +/- 1.12 mmol/L) were higher than that in group S1 (5.13 +/- 1.06 mmol/L) (P < 0.05, P < 0.05). The serum apo B100, C II, C III and E levels in groups S2 and S3 were significantly higher as compared with that in the normal group (P < 0.01, P < 0.05). The correlation analysis indicated that there was a positive correlation of BG with serum TG in groups S1, S2 and S3 whose correlation coefficients (r) were 0.1800, 0.1436 and 0.3212 respectively (P < 0.001); and a negative correlation with HDL-C levels in groups S2 and S3 (r = -0.2534 and -0.1477, P < 0.001). The plasma glucose levels were positively correlated with apo B100, C III and E levels in groups S1, S2 and S3, P < 0.001), with apo C II in groups S1 and S2 (P < 0.001, P < 0.01); and negatively correlate to apoA I only in group S2 (r = -0.1182, P < 0.01). CONCLUSION: The results suggest that serum TG, TC, HDL-C, apoB100, C II, C III and E levels be the main factors that related to plasma glucose levels.

Aged↗

PubMed2001

[Effects of insulin and transforming growth factor-beta on alkaline phosphatase activity and total protein content in human periodontal ligament cells].

OBJECTIVE: The aim of this study is to evaluate the biological effects of insulin and/or transforming growth factor (TGF-beta) on alkaline phosphatase (ALPase) activity and protein synthesis of periodontal ligament (PDL) cells in vitro. METHODS: The fifth passage of PDL cells were cultured in a 5% humid CO2 environment at 37 degrees C by using Dulbecco minimum essential medium (DMEM) supplemented with 10% fetal bovine serum (FBS) for 24 hours, and then insulin, TGF-beta, and insulin and TGF-beta were added respectively in a medium of 1% FBS. After 5 days, their ALPase activities were measured by using a kinetic method, and the total protein content was measured by using Coomassie brilliant blue staining. RESULTS: Both insulin and TGF-beta significantly increased the ALPase activity and the total protein content of PDL cells at the concentrations from 1.0 to 100 U/L, and of 0.1-100 micrograms/L. The most optimal concentrations of insulin and TGF-beta were respectively 10 U/L and 1 microgram/L. The synergistic effect of insulin (10 U/L) and TGF-beta (1 microgram/L) was observed (P < 0.01). CONCLUSION: The results suggest that insulin and/or TGF-beta may promote the differentiation and protein synthesis of PDL cells. The combination of them may be valuable in the regeneration of periodontal tissue.

Adolescent↗