Biomedical subjects

Z Wang

Publications and source records attributed to Z Wang.

At least 469 records · Page 26Linked to original sources

PubMed2001

Effect of fetal spinal cord graft with different methods on axonal pathology after spinal cord contusion.

OBJECTIVE: To investigate the effect of fetal spinal cord (FSC) graft with different methods on axonal pathology and neurological function recovery after spinal cord injury (SCI). METHODS: Forty Wistar rats were divided into 4 groups. In Group A, the spinal cord was injured and hemisected. In Group B, fetal spinal cord (FSC) was transferred into the injured site. In Group C, after having done as Group B, the upper and lower spinal nerve roots were anastomosed. And in Group D, after having done as Group B, the pedicled omentum was transferred into the hemisection cavity. At 6 weeks after operation, light and electronic microscopes were used to examine the axonal pathology. The neurological function was assessed with inclined plane tests in the open field. The number of axons was quantitated by a computer image analysis system. RESULTS: A greater loss of axons was observed in Group A than that of other groups at 6 weeks. The sequence of the reduced rate of the axons was as following, Group A>Group B>Group C>Group D (P<0.05). The remaining axons were paralleled with the significant improvement in neurological function recovery of the rats. CONCLUSIONS: It indicates that FSC and pedicled omentum grafts after SCI can protect the axons and promote the neurological function recovery of the rats.

Animals↗

PubMed2001

Allogeneic bone nail for articular fractures.

OBJECTIVE: To investigate the effect of allogeneic bone nail on articular fractures. METHODS: Ninety-one patients with cancellous bone fracture involved in various joints were fixed with the bone nail or the bone screw. No immunosuppressive agent was used in this series. The average period of follow-up wa s 18 months. RESULTS: All patients except one healed without displacement an d the healing time was within 1-3 months. Joint function was partially limited in 6 patients and others were functioning well. CONCLUSIONS: Allogeneic bone nail possesses a good biological compatibility and intensity, thus provides a rigid fixation. Being completely absorbed and ossificated, thus obviating the need for secondary operation, is a suitable internal fixation material for the treatment of articular fractures.

Acetabulum↗

PubMed2001

[The biological effect of verapamil on hypertrophic scar fibroblast].

OBJECTIVE: To study the mechanism of calcium channel antagonist Verapamil on the treatment of hypertrophic scar and explore the possibility of further clinical application. METHODS: After six strains of HSFB were cultured in vitro, we investigated HSFB proliferation by MTT method, investigated HSFB collagen synthesis by 3H-proline uptaken and Hydroxyproline colorimetric analysis, and investigated collagen gene expression by Northern Blot. RESULTS: Verapamil can inhibit HSFB proliferation, collagen synthesis and gene expression by a dose-depended manner, especially treated with 100 mumol/L Verapamil. CONCLUSION: By inhibiting I, III procollagen gene expression, Verapamil can inhibit the formation of hypertrophic scar.

Adolescent↗

PubMed2001

[A study on the resources coping with occupational stress in teachers].

OBJECTIVE: To explore the status of the resources coping with occupational stress in teachers. METHODS: Occupational stress inventory revised edition (OSI-R) was used to measure their occupational stress, strain and psychological coping resources for 1,460 teachers in primary and secondary schools and 319 non-teacher intellectuals. Analyses were focused on coping resources of teachers. RESULTS: The higher the level of coping resource of teachers, the lower the personal strain in them, with an inverse correlationship. Coping resource in the teacher group was significantly higher than that in the non-teacher group, with scores of (130.4 +/- 18.2) and (126.9 +/- 19.1), respectively. Coping resource in teachers decreased with the increase of age, with the scores from (134.1 +/- 18.1) in the group aged less than 30 to (128.5 +/- 17.5) in the group aged 50. Coping resource in the female teachers was significantly higher than that in the male teachers, with scores of (131.4 +/- 18.3) and (129.4 +/- 18.1), respectively. Coping resource in the primary school teachers was significantly higher than that in the secondary school teachers, with scores of (132.7 +/- 18.1) and (128.5 +/- 18.1), respectively. CONCLUSION: It is important to enhance teacher's coping resource, especially for the male teachers in the secondary schools, for improving their teaching efficiency.

Adaptation, Psychological↗

PubMed2001

[Mechanism of contour hedgerow's control for non-point pollution in the semiarid region in northwest Hebei Province].

Slope nutrient loss is an important type of non-point pollution. The mechanism of non-point pollution control by using plenty of natural observed data in Hilly Loess region in Northwest Hebei Province, as well as combating with the large-scale artificial simulation rainfall in the field slope was analyzed. The study results show that the critical slope length was 10-15 m on the condition of rainstorm, which led to the generation of rill and made the erosion amount went up obviously. The study show that the nutrient loss in sediment was the main type of non-point pollution, the contour hedgerow the process of non-point pollution by controlling the nutrient loss in sediment, then the study got the erosion control model of the contour hedgerow.

China↗

PubMed2001

[A study on the risk factors of cerebral infarction as complication of type 2 diabetes mellitu patients].

OBJECTIVE: Cerebral infarction, a common acute cereovascular disease, is one of the main complications of type 2 diabetes mellitus (DM). This paper is to explore the risk factors of cerebral infarction among type 2 DM patients. METHODS: A pair-matched case-control study was carried out. Data was analyzed with conditional logistic regression. RESULTS: One hundred and ten type 2 DM patients with cerebral infarction and controls were selected respectively. Results showed that higher educational level and physical exercises lowered the risk of cerebral infarction among type 2 DM patients. Higher HDL-C and blood sugar under control could also lower the risk of developing cerebral infarction among type 2 DM patients. The above factors seemed to have protective functions. Excessive salt intake and snoring increased the risks of cerebral infarction development among type 2 DM patients. Hypertension served as an independent risk factor while atrial fibrillation and hyperinsulinemia were important risk factors. High LDL-C, Apo-A, Lp(a), Fg increased the risk of cerebral infarction among type 2 DM patients. The study did not discover the relationship of smoking, drinking, oral contraception, menstrual and obstetrical history, TC, TG, Apo-A and cerebral infarction among type 2 DM patients. CONCLUSION: To prevent cerebral infarction among type 2 DM, measures need to be taken as control of salt intake; eating more vegetables; regular physical exercises; control of blood sugar, blood pressure and Hyperinsulinemia.

Adult↗

PubMed2001

[A clinicopathologic study on 9 cases of epithelioid hemangioendothelioma].

OBJECTIVE: To study the clinicopathological features of epithelioid (histiocytoid) hemangioendothelioma (EHE). METHODS: 9 cases of EHE were examined by light microscopy and immunohistochemistry, 1 case with ultrastructural observation. RESULTS: The patients' age ranged from 16 to 47 years (mean 32 years). The lesions occurred in both sexes and presented at a variety of sites including head and facial area (4 cases), upper limbs (3 cases) and lower limbs (2 cases). The morphological characters were: (1) "epithelioid" or "histiocytoid" tumor cells; (2) rounded or polygonal tumor cells arranged in nests, cords and irregular patterns; (3) the tumor cells were embedded in myxoid or hyaline matrix; (4) primitive lumen formation within a single tumor cell (intracytoplasmic lumens); (5) lack of pleomorphism, mitotic activity and necrosis; (6) presence of spindle cell hemangioendothelioma in some cases; (7) osteoclast like giant cells scattered throughout the entire lesion were observed in one case. Immunohistochemistry studies on 7 cases found that tumor cells were positive for vimentin (7/7), factor VIII-RAg (5/7), CD31 (5/7), CD34 (5/7), cytokeratin (2/7), CD68 and alpha-AT (1/7). Ultrastructural study of 1 case demonstrated Weibel-Palade bodies and an abundance of intermediate filaments in the cytoplasm. The follow-up of 6 cases found that 3 cases developed local recurrences but none of them metastasized. CONCLUSIONS: EHE is a distinctive low-grade sarcoma whose etiology remains unknown. Recognizing its morphological characters is important to avoid confusion with other similar lesions.

Adolescent↗

PubMed2001

[Analysis of loss of heterozygosity on chromosome 6 in human prostate carcinoma and prostatic intraepithelial neoplasia].

OBJECTIVE: To detect the significance of loss of heterozygosity (LOH) on chromosome 6 in prostate carcinoma and high grade prostatic intraepithelial neoplasia (PIN). METHODS: Pure DNA was obtained from prostate neoplasms and normal tissues after tissue microdissection. LOH of chromosome 6 was detected by PCR based microsatellite polymorphism analysis technique using 20 pairs of microsatellite primers in 10 prostate carcinoma cases and 10 high grade PIN cases. RESULTS: Allelic loss at one or more loci was observed on chromosome 6 in 8 of 10 prostate carcinoma cases. 6q21-6q23 and 6q25-6q27 were two of LOH high frequency regions. 5 high grade PIN cases had LOH detected on chromosome 6. CONCLUSIONS: Two high frequency LOH regions were detected on chromosome 6 of prostate carcinoma. Cyclin C, IGF2R genes were two candidate tumor suppressor genes located in these two regions, they may be involved in the initiation and progression of prostate cancer.

Aged↗

PubMed2001

[An experimental study on the construction of composite graft with epithelia and fibroblasts on collagen sponge].

OBJECTIVE: Primarily cultured human epithelia and fibroblasts were inoculated on collagen sponge to construct composite skin substitute. METHODS: Epithelia and fibroblast asts from human foreskin were primarily cultured. Fibroblasts (1 x 10(5)/cm(2)) were inoculated on collagen sponge after digestion and cultured for 3 days prior to inoculation of epithelial cells (2 x 10(5)/cm(2)) to construct composite skin grafting substitute, and it was then grafted onto full skin loss (FSL) wounds of nude mice. Collagen sponge membrane lacking cell inoculation was used as control. The wound healing was evaluated by measuring the residual wound area. Tissue samples were harvested and examined by means of histology, immunohistochemistry and electron microscopy. RESULTS: Epithelia and fibroblasts could be cultured in vitro on the carrier collagen sponge. After being grafted onto the wound, the epithelia and fibroblasts wound further proliferated and differentiated. The composite skin could stick to the carrier fairly well. There exhibited less epidermal scar proliferation and satisfactory contour of the neoskin. Integral basal membrane of the skin could be identified by immunohistochemical staining. The quality and speed of wound healing with the composite skin substitute were superior to that with collagen sponge only. CONCLUSION: The composite grafts constructed by epithelia and fibroblasts on collagen sponge could be a potential neotype composite skin substitute for the repair of FSL.

Animals↗

PubMed2001

[Study of C807T polymorphism of the platelet collagen receptor alpha2 gene coding sequence in Suzhou Han population].

OBJECTIVE: To study the C807T polymorphism of the platelet collagen receptor alpha(2) gene coding sequence in Suzhou Han population. This polymorphism was associated with the expression density of integrin alpha(2)beta(1) on the platelet surface. METHODS: An amplified 1332 bp fragment of human alpha(2) gene from 110 unrelated individuals was analyzed by Bgl II and Ase I restriction assay. The resulting products were electrophoresed in 8% polyacrylamide gels and stained with ethidium bromide. RESULTS: The allelic frequencies were 0.291:0.709 for T807 allele: C807 allele in Suzhou Han population. The total theoretical heterozygous rate was 41%. The genotypic frequencies were 0.018:0.546:0.436 for homozygous 807T allele: heterozygous 807C/T allele: homozygous 807C allele. CONCLUSION: The C807T polymorphism of the platelet collagen receptor alpha2 gene in Suzhou Han population was different from that observed in other populations.

Asian People↗

PubMed2001

[Dialectic research on "shu pu" (referring a symptom) and "shu pu" (referring a place)].

The name of "shu pu" come from Shen shi fang (Prescriptions of Master Shen), which was quoted by Wai tai mi yao (Medical Secrets of an Official), compiled by Wang Tao of the Tang dynasty. In the successive processes of Zheng lei ben cao (Classified Materia Medica) and Ben cao gang mu (Compendium of Materia Medica), it was mixed up with "shu pu", a symptom, in Su wen ci jin lun (On Banning of Acupuncture in Plain Questions), and "shu pu", a place, in Zhen jiu jia yi jing (A -- B Classin of Acupuncture and Moxibustion). In fact, "shu pu" was the meat of rats with no processing, which can be used externally or administered orally to treat injury due to metallic tools and abscess.

China↗

PubMed2001

[Pharmacodynamics study of suanzaoren decoction extracted by different technological process].

OBJECTIVE: To select the optimal technological process for Suanzaoren decoction. METHOD: The effects of extracts from different technological processes on spontaneous motion and sleep time in mice induced by pentobarbital sodium (subthreshold dosage) were studied. RESULTS: Both the decoction and 95% alcohol extract of Suanzaoren possess sedative and hypnotic effects. CONCLUSION: Suanzaoren aqueous decoction was selected as the optimal technological process.

Animals↗

PubMed2001

[Diagnostic significance of measurement of the receptor for urokinase-type plasminogen activator on granulocytes and in plasma from patients with paroxysmal nocturnal hemoglobinuria].

OBJECTIVE: To investigate the expression levels of the receptor for urokinase-type plasminogen activator (uPAR) on granulocytes and the soluble uPAR (suPAR) level in plasma from patients with paroxysmal nocturnal hemoglobinuria (PNH) and its clinical application in the diagnosis of this disorder. METHODS: Expression of uPAR, CD55 and CD59 was measured on granulocytes by using flow cytometry (FCM) and the level of suPAR in plasma by using immunoradiometric assay (IRMA) in 20 patients with PNH, 59 anemic patients with diagnoses other than PNH (18 with autoimmune hemolytic anemia, 6 with other hemolytic anemias, 26 with aplastic anemia and 9 with iron deficiency anemia) and 21 healthy individuals. RESULTS: In PNH patients, both the mean fluorescence intensity and percentage of positive fluorescence-activated granulocytes of uPAR, CD55 and CD59 were remarkably decreased, while in anemic patients with diagnoses other than PNH the results were not different when compared with those of the healthy individuals. The expression level of uPAR was reduced to the same extent as that of CD55 and CD59 on the PNH-affected granulocytes. In addition, some peak shape abnormalities (double peaks and/or peak tailing) in the histogram of fluorescence intensity were also found in PNH patients. The suPAR concentration of PNH plasma was (4.04 +/- 2.47) micrograms/L, being higher than that of the healthy individuals (1.73 +/- 0.96) micrograms/L (P < 0.01). And the percentage of positive fluorescence-activated granulocytes was inversely associated with the plasma suPAR level in PNH patients (r = -0.79, P < 0.01). CONCLUSION: It is suggested that measurement of expression level of uPAR on granulocytes with FCM and suPAR in plasma with IRMA would be alteanative specific techniques for the diagnosis of PNH.

Adolescent↗

PubMed2001

[A study for the relationship between serum calcitonin gene-related peptide and coronary arteriopathy].

OBJECTIVE: To investigate the relationship between serum calcitonin gene-related peptide (CGRP) and coronary angiopathy. METHODS: In a cross-sectional study, serum CGRP levels of 119 patients with coronary heart disease (CHD), 78 with non CHD and 39 healthy subjects were measured with radioassay method. The information on conventional risk factors were collected by interviews. RESULTS: The development of coronary angiopathy was correlated with smoking, diabetes and aging. The mean serum CGRP level was significantly lower in CHD than in NCHD patients [(35.5 +/- 48.8) mumol/L vs (63.8 +/- 47.3) mumol/L, P < 0.01). There was no difference between the mean serum CGRP level in NCHD patients and healthy controls. By multivariate logistic regression, the odds ratios (OR) of aging, smoking and diabetes were all > or = 1 (P < 0.01), that means they are independent risk factors for the development of coronary angiopathy. The OR of CGRP was < or = 1, indicating that it was an independent protective factor. CONCLUSIONS: It is shown that aging, smoking and diabetes are all independent risk factors for the development of angiopathy, while CGRP is an independent protective factor for the development of coronary angiopathy.

Aged↗

PubMed2001

[A study on the change of caries activity of children with fixed orthodontic treatment].

OBJECTIVE: To investigate the change of caries activity in children with fixed appliance. METHODS: 30 children of permanent dentition were treated with edgewise appliance. CAT was used to test the degree of caries activity before the orthodontic treatment, at the first week, at the first month, at the third month and at the sixth month of appliance bonding respectively. RESULTS: Compared with pre-treatment, there was a significant difference of CAT at the first, third and sixth month of treatment; compared with the first week of treatment, there was also a significant difference of CAT at the third and sixth month of treatment. CONCLUSIONS: The CAT degree increases in children with fixed orthodontic appliance and these children are more sensitive to dental caries.

Adolescent↗

PubMed2001

[The influence of surface roughness on bacteria adhesion on titanium].

OBJECTIVE: To investigate the correlation between surface roughness and bacteria adhesion. METHODS: Surface roughness of four abutments were determined by Talysurf/6p-120 topographer. Ra, Rz, Rp, Rv, Ry and Sm values were gathered. Commercial pure titanium and titanium alloy discs, S. mutans and A. viscosus were used in adhesion experiment. RESULTS: Ra values (in microns) of the tested abutments were: 0.1556, 0.2073, 0.6976, and 0.3811. No statistical difference was observed in bacterial quantity when Ra < 0.4528 microns and Rz < 3.3882 microns in adhesion experiment. CONCLUSIONS: The surface roughness of Ra < 0.4 micron and Rz < 3.4 microns is recommended for implant abutment.

Actinomyces↗

PubMed2001

[Shoulder pain after laparoscopic cholecystectomy].

OBJECTIVE: To detect the mechanism of shoulder pain after laparoscopic cholecystectomy(LC). METHODS: Ninety patients who had receive LC were randomly divided into three groups (each 30 patients): group A, no management after LC; group B, absorption of residual CO2 after LC; group C, O2 insufflation for three times followed by complete absorption of CO2 after LC. PO2 and PCO2 before and after LC were recorded. Postoperative shoulder pain was assessed with shoulder pain's rate and level. RESULTS: The PO2 difference of group C between before and after LC was significant as compared to groups A and B(P < 0.05). Postoperative shoulder pain developed in 13 patients (43.33%) of group A, in 8 (26.67%) of group B, and 21 (70%) of group C (P > 0.05 A vs B; P < 0.05 A vs C; and P < 0.01 B vs C). The shoulder pain's level was higher in group C than in groups A and B. CONCLUSIONS: Direct irritation to the diaphragm due to the residual CO2 pneumoperitoneum is not the main cause of shoulder pain. The excessive traction of the triangular ligament and over-stretching of the diaphragmatic fibers due to insufflation are the main cause of shoulder pain.

Carbon Dioxide↗

PubMed2001

[Establishment and biologic characterization of human hepatocellular carcinoma cell line HCC-9903].

OBJECTIVE: To establish a new hepatocellular carcinoma (HCC) cell line and provide a new and ideal experimental model. METHODS: The cell line designated as HCC-9903 was derived from a surgical specimen of HCC. After primary culture, its biologic characteristics were studied under light and electron microscope and by determination of doubling time and growth curve, culture in soft agar, flow cytometry(FCM), chromosome analysis, detection of AFP, HBV and telomerase, agglutination test of concanavalin (ConA) as well as inoculation in nude mice. RESULTS: The first passage was found on the 29th day. Morphological study demonstrated its malignant characteristics. Chromosome analysis revealed a chromosome number ranging from 49 to 135, with a model number of 52 to 67. Aberrant chromosomes 1 q(i) and t (6,11) were its chromosome markers. The cell line produced tumors after subcutaneous transplantation into nude mice. CONCLUSION: According to the rules of establishing a cell line in vitro, HCC-9903 has been proved to be a new cell line of human HCC carcinoma, and more experimental methods have been used to study its biological characteristics.

Animals↗