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Biomedical subjects

Z M Andreeva

Publications and source records attributed to Z M Andreeva.

At least 55 records · Page 3Linked to original sources
PubMed1976

[Taxonomic subdivision of salmonellae].

Elaboration of an objective approach to the classification of salmonellae by the biochemical characteristics is of great theoretical and practical interest. The authors carried out a taxonomic analysis of 966 salmonellae and 241 arizonae by 26 biochemical signs. Similarity between all the couples was estimated on the computer M-220; group analysis was carried out by 3 methods with the successive reduction of the group level by 2%. The grouping results are presented on a dendrogram. Analysis of the dendrogram demonstrated that 4 taxons were distinctly traced at the level of 94%, which were united with one another at the level of 93%. The whole group formed at the level of S = 76%, which served as the genus level of the salmonella union. Arizona group was connected with the bond between the II and IV subgenera. A method of numerical taxonomy provied arizona and salmonella to be undoubtfully affiliated, this permitting to regard arizona as the subgenus II of the Salmonella genus.

Computers↗
PubMed1975

[Obtaining specific non-adsorbed sera to Haemophilus influenza for the agglutination test on glass].

Type-specific agglutinating sera to capsular antigenas of H. influenzae were recovered (from 1:3200 to 1:25600). With the given immunization scheme there formed mostly IgG immunoglobulins, and only insignificant amount of IgM (separation of G-200 Sephadex gel). IgG were found to be responsible for the type-specificity of the sera to bacteria, whereas the cross reactions within the H. influenzae genus were connected connected with the IgM. Use of the sera heated at various temperature regimens offered a possibility of obtaining highly specific agglutinating sera with the aid of which express serodiagnosis of the isolated H. influenzae strains was possible.

Adsorption↗
PubMed1975

[Characteristics of some species of microorganisms related to the genus of hemoglobinophils].

The saccharolytic capacity in respect to 14 carbohydrates, the lipolytic activity, the presence of urease, catalase, galactosidase, the formation of hydrogen sulphide and indol and also serological properties with the species agglutinating sera were studied in the representatives of the genus of hemoglobinophilic microbes: H. influenzae, H. parainfluenzae, H. aegiptius, H. haemolyticus, H. aphrophilus. There were revealed differences in the individual representatives of the genus by the enzymatic activity and serological properties. Thus, representatives of H. influenzae possessed urease activity, but all of them lacked galactosidase. H. aegiptius possessed urease and galactosidase, and H. parainfluenzae, H. haemolyticus and H. aphrophilus--galactosidase of high activity, but no urease. Representatives of each of the species were agglutinated by homologous sera only.

Agglutination Tests↗
PubMed1975

[Analysis of a group of Corynebacterium by numerical taxonomic methods].

Strains (29) of Corynebacterium genus were studied with the use of the method of numerical taxonomy. 48 characteristics (including morphological, tinctorial, cultural and biochemical ones) were analyzed. All the strains tested proved to belong to the same taxon. The present method permitted to reveal various biotypes within the group studied.

Computers↗
PubMed1975

[Isolation and identification of Neisseria in bronchial asthma].

A study was made of 29 strains of neisseria isolated from the mucosa of the pharynx and the bronchi of patients suffering from infectious-allergic bronchial asthma of different severity. Comparison of the strains by 22 tests (including the assessment of the morphological, biochemical and other signs) permitted to establish the species of neisseria with some probability. The species reference was determined in 23 cultures. N. perflava was isolated in 10 cases, N. subflava--in 6, N. flava--in 3 cases and N. sicca--in 4 cases, 2 strains corresponded to apigmentary N. cinerea. The species was not established in 4 cultures. There was found no connection between a definite microbial association and the species of neisseria and the severity of the disease. Pigment species of neisseria should be tested as diagnostic allergens.

Asthma↗
PubMed1975

[Use of the oblique illumination method for characterizing N. meningitidis cultures].

The authors used the method of oblique illumination for detection of the state of the population of meningococcus cultures. In studying 48 strains there were revealed three types of fluorescence of the colonies: bright orange with a transition into greenish-light blue along the lower margin (the I type), bright light blue with a narrow orange or green upper margin (the II type) and greyish-light blue colonies (the III type). The type of fluorescence was not associated with the sero-group specificity. Populations of meningococcus cultures, depending on conditions and duration of growth on the nutrient medium could consist both of the colonies with the same type of fluorescence, and also represent a combination of colonies with a different type of fluorescence. The colonies of the I and II types of fluorescence had marked group specificity, but mutation occurred during passage and the III type of fluorescence formed with a loss of serological specificity. Continuous selection of the colonies of the I and the II types of fluorescence permitted to preserve the population with specific properties necessary for production of diagnostic and vaccine preparations for a long time.

Antigens, Bacterial↗
PubMed1975

[Assessment of the condition of the population of gonococcal cultures].

Application of the method of oblique illumination permitted to reveal nonhomogeneousness of the cultures isolated from the patients with a different (acute and torpid) course of the disease. Passage of the freshly-isolated cultures on the nutrient media led to the appearance of mutants characterized by the fluorescence of the third type which also characterized all the 48 museum cultures stored for a long time on the nutrient media. The method suggested permits not only to evaluate the gonococcal cultures condition but also to carry out their directed selection.

Bacteriological Techniques↗