[Basic principles of identifying microorganisms in clinical bacteriology].
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Biomedical subjects
Publications and source records attributed to Z M Andreeva.
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The effect of thimerosal used at different concentrations on the growth of S. haemolyticus, strain Dick I, and A. faecalis, strain 415, has been studied. A. faecalis, strain 415, in contrast to S. haemolyticus, strain Dick I, has been shown to be highly sensitive to thimerosal. The growth and neutralizing properties of a number of lots of thyoglycol medium have been studied with the use of the above-mentioned strains. The advantages of A. faecalis, strain 415, over S. haemolyticus, strain Dick I, for the evaluation of these properties of thioglycol medium have been established. A. faecalis, strain 415, can be recommended for use as a test strain for evaluating the quality of thioglycol medium instead of S. haemolyticus, strain Dick I.
A paper indicator system has been developed in the USSR for the accelerated identification of vibrios and proposed for the identification of enterobacteria. This system consists of paper discs or strips impregnated with definite substrates (carbohydrates, polyatomic alcohols, amino acids, etc.) in combination with an indicator and stabilized with the polymer coating. Two kits for the identification of enterobacteria to the level of the genus (kit A for 9 tests) and to the level of the species (kit B for 25 tests) are presented.
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Possible use of the dry nutrient medium manufactured in the USSR for the assay of aminoglycoside antibiotic activity with the agar diffusion method was studied. The optimal conditions for the antibiotic activity assay on this medium were developed. The dry nutrient medium may be used for the activity assay of the aminoglycoside antibiotics, i. e. streptomycin sulfate, dihydrostreptomycin sulfate, neomycin sulfate, monomycin and gentamicin sulfate.
The comparative study of Neisseria strains, isolated from the bronchi and the pharynx of healthy persons and infectious asthma patients, was made. Nonpathogenic Neisseria isolated from bronchial asthma patients were represented by the same species as those in the pharyngeal flora of healthy persons. Only N. perflava occurred in the pharynx and the bronchi of infectious asthma patients twice as often as in healthy persons. Neisseria colonies in the R-form were shown to prevail in the cultures obtained by the inoculation of the smears taken from asthma patients.
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Differential diagnosis of enterobacteria is based on the determination of beta-galactosidase enzyme, hydrolyzing lactose in the nutrient substrates; however, the tests suggested for its determination are time consuming and their use in practice is limited. Dry nutrient medium prepared by the authors containing o-nitrophenyl-betaD-pyranoside was tested on 1625 strains of enterobacteria in comparison with the Le Minor's ONPS test used at present. The results of beta-galactosidase determination by the, mentioned methods proved to coincide (significance level greater than 95%); this permits to recommend the method described--in dry nutrient medium--as a differential-diagnostic test for the identification of enterobacteria.
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