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Biomedical subjects

Z Liu

Publications and source records attributed to Z Liu.

At least 1,351 records · Page 75Linked to original sources
PubMed1990

[Quantitative studies on nuclear DNA content and morphological features in inverted papillomas of the nasal cavities and paranasal sinuses].

DNA contents and morphological features (including nuclear area, nuclear perimeter and nuclear volume) were quantitatively measured by image analysis in 38 inverted papillomas (IP) of the nasal cavities and paranasal sinuses. Results showed that DNA contents and morphological parameters increased markedly than those of the normal cells, but were lower than those of malignant cells, indicating IP may be a borderline tumor in proliferative state. There was significant correlation between the DNA ploidy and recurrence rate of IP, aneuploid tumors have a higher recurrence rate than diploid and multi-euploid tumors. Quantitative measurements of DNA contents and morphological parameters very important for early detection of recurrence and malignant change in IP.

Adolescent↗
PubMed1990

[Experiment study of adriamycin-induced nephrotic syndrome in rats].

A single intravenous injection of adriamycin (5 mg/kg) into rats caused the full expression of nephrotic syndrome characterized by heavy proteinuria, hypoalbuminemia, hypercholesterolemia, thoracic and ascitic fluid; swelling and fusion of foot processes of epithelial cells could be observed under electron microscope; histologic examination by light microscopy did not reveal any significant changes. The features of the model in clinic and pathology were very similar to those described humans with minimal change nephrotic syndrome. The observation in the various periods of the model indicated that the ultrastructure changes in epithelial cells occurred prior to the onset of proteinuria. The present study, combined with the results of quantitative analysis with image analyzer for alteration of glomerular polyanions, suggests that both morphologic changes and proteinuria may be the consequence of a common primary event that is the loss of glomerular polyanions. The model has the advantages of being rather simple and convenient; providing lasting proteinuria and pathological changes in stable condition; and having high reproducibility and a sufficient supply of the drug used.

Animals↗
PubMed1990

[A comparative experimental study of side-to-side and end-to-side mesocaval shunt].

In this study, the side-to-side (MCS-SS) and end-to-side (MCS-ES) mesocaval shunt were performed in two groups of thioacetamide (TAA)-induced cirrhosis of rats. The anastomotic stoma was made equal to the calibre of mesenteric veins. It was found that the portal vein pressure after MCS-SS was lower than that after MCS-ES owing to the more effective drainage of both extra- and intrahepatic portal blood flow. It was also found that with MCS-SS, some inflow from the superior mesenteric vein was reserved to irrigate the liver and the content of insulin in the portal vein was not affected. The authors came to the conclusion that MCS-SS was superior to MCS-ES for less likely causing post-shunt encephalopathy and the deterioration of hepatic function.

Animals↗
PubMed1990

[Snail distribution and susceptible zones of schistosomiasis in endemic areas around Poyang Lake].

To understand the situation of snail distribution and to explore the criteria of susceptible zones of schisosomiasis in endemic areas around Poyang Lake, a total of 1,222,910 mu of marshland in lake regions were surveyed for three years from 1982 through 1984 by 400 skillful observers. This article reports the relation between mean density of infected snails and incidence of acute schistosomiasis in marshland. We divided all susceptible marshlands of Poyang Lake area into three types, and found that about one fourth of the area belongs to the first type of susceptible zones, i.e., with high risk to schistosomiasis infection. Longitudinal observation showed that the types of susceptible zones changed very little unless measures of schistosomiasis control were taken. The results of field study suggested that we should put stress of schistosomiasis control on the first type of susceptible marshlands of Poyang Lake in future.

Animals↗
PubMed1990

[Effect of injection of thyrotropin-releasing hormone into nucleus accumbens on the pain discharges in nucleus parafascicularis of rat thalamus].

Glass microelectaod recording method was used to investigate the effect of thyrotropin-releasing hormone (TRH) into nucleus accumbens, nucleus amygdaloid or nucleus caudate-putamen on unit discharges of pain-excitation neurons (PEN) in nucleus parafascicularis of thalamus in rats. The results showed that: (1) TRH injected into the nucleus accumbens results in a significant inhibition of pain discharges of PEN in nucleus parafascicularis. But injection of TRH into nucleus amygdaloid, nucleus caudate-putamen had no significant effect. (2) Pretreatment with auropine abolished the inhibitory effect of TRH. (3) Pretreatment with haloperidol also abolished the inhibitory effect of TRH. (4) Pretreatment with naloxone, propranolol or phentolamine did not affect the inhibitory effect of TRH. The results mentioned above suggested that nucleus accumbens could be a special area in response to TRH and the effect of TRH seems to be involved in both cholinergic M-receptor and dopamine receptor.

Amygdala↗
PubMed1990

[Effect of acupuncture and moxibustion on the high density lipoprotein cholesterol in simple obesity].

For the purpose of understanding regulatory effect of acupuncture and moxibustion, we have observed the changes of the obese indices and the lipid indices in 196 sample obesities before and after the acupuncture and moxibustion. The results showed that a good therapeutic effect of acupuncture and moxibustion on obesity was obtained. At the same time, there are the benign regulatory effect of acupuncture and moxibustion on the lipid metabolism and high density lipoprotein cholesterol.

Acupuncture Points↗
PubMed1990

[Autoradiographic visualization on the role of central 3H-5-hydroxytryptamine in acupuncture analgesia].

The role played by central 5-hydroxytryptamine (5-HT) in electro-acupuncture analgesia has been studied in rats by means of autoradiography with isotopic tracers 3H-5-HT. The purpose of the present study is to determine the localization of 3H-5-HT in the midbrain raphe nuclei and in the mesencephalon aqueduct as well as periaqueductal gray matter. Parallel experiments were studied by freezing microautoradiographic method and histo-fixative microautoradiographic method. The analgesia effect of acupuncture can be enhanced or lowered by the increment or the decrement of the 5-HT level in the midbrain raphe nuclei and in the mesencephalon aqueduct as well as periaqueductal gray matter. The results of the present experiments show that, when the rats were subjected to electro-acupuncture analgesia. The microautoradiographic intensities of 3H-5-HT both in the midbrain raphe nuclei and in the areas of mesencephalon aqueduct were significantly increased. It may be observed that the release of 5-HT in these regions of the brain is accelerated during acupuncture analgesia. From this it can be concluded that the midbrain raphe nuclei and the mesencephalon aqueduct as well as the periaqueductal gray matter are closely related to acupuncture analgesia. The results imply that 5-HT in these areas may be one of the most important neurochemical agents mediating acupuncture analgesia.

Acupuncture Analgesia↗
PubMed1990

[Effects of Ligusticum wallichii on the plasma levels of beta-thromboglobulin, platelet factor 4, thromboxane B2 and 6-keto-PGF1 alpha in rabbits under acute experimental cerebral ischemia].

By occluding the bilateral carotid arteries of rabbits to produce bilateral partial cerebral ischemia, and by using RIA and ELISA to measure the levels of Beta-thromboglobulin (beta-TG), platelet factor 4 (PF4), thromboxane B2 (TXB2) and 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) in plasma, the authors found that the levels of beta-TG, PF4 and TXB2 in plasma had significantly increased (P less than 0.01), but the level of 6-keto-PGF1 alpha in plasma showed no change (P greater than 0.05) after cerebral ischemia appeared. The results of the Ligusticum wallichii (Ligusticum) pre-treatment to the test-group showed that the levels of beta-TG, PF4 and TXB2 in plasma had significantly decreased (P less than 0.01), and the level of 6-keto-PGF1 alpha in plasma had significantly increased (P less than 0.05). This suggested that the Ligusticum treatment could effectively inhibit the platelet activation in vivo and correct the TXA2-PGI2 imbalance in blood after cerebral ischemia. In this study, some new approaches were explored to explain the mechanisms of Ligusticum for preventing and treating cerebral ischemia.

6-Ketoprostaglandin F1 alpha↗
PubMed1990

[The effect of heat shock on mRNA expression in human T lymphocytes].

Total mRNA was extracted from activated T lymphocytes and Jurkat cells with and without heat shock, and then used for alpha-32P-labeled 1st strand cDNA synthesis with reverse transcriptase. DNA restriction fragments or cloned vectors of five oncogenes (abl, myc, myb, fos, Ki-ras) and of IL-2, IL-2 receptor, T-cell receptor beta-chain and transferrin receptor were dotted onto nitrocellulose filters. Hybridization results showed that the expression of c-myc and TfR mRNA was much lower in heat-shocked cells than in their normal counterparts. However IL-2 and Ki-ras mRNA increased after heat shock. Possible explanations for the results are discussed.

Gene Expression Regulation, Leukemic↗
PubMed1990

[Significance of IgM deposition in IgA nephropathy: an appraisal based on renal re-biopsy].

Repeated renal biopsies were performed in 30 patients with primary IgA nephropathy at intervals of 6-55 months during 1983-1988. Histological and immunopathological studies were conducted for each patient and comparisons were made between the 1st and 2nd biopsy. It was found that the frequency of IgM deposition in the renal glomeruli increased significantly during the course of illness from 11/30 to 21/30, while the IgA, IgG and complement (C3, C4, C1q) deposition remained rather stationary. Comparing the group of patients who were deficient in IgM deposits with those who procured this particular Ig during the illness, there were striking differences in the frequency of occurrence of nephrosis, glomerulosclerosis, severe tubular-interstitial lesion and renal insufficiency. The frequency was much higher in the IgM deposited patients In conclusion: (1) Deposition of IgM might happen during the course of IgA nephropathy as a secondary phenomenon. (2) Presence of IgM in the glomeruli immune deposits denote a poor prognosis for patients of IgA nephropathy.

Adolescent↗
PubMed1990

[Sampling survey of mentally deficient people in Sichuan].

From first April to 15th May 1987, 145,201 people were investigated by using the ways of layer, equal probability and mass sampling. Two thousand three hundred and forty two of them were confirmed to be mentally deficient. The incidence was 16.13%. It was estimated that 1,665,000 people have mental deficiency in Sichuan. The estimated range of reliability of 95% was 1,528,000-1,801,000. One million and three hundred ninety three thousand of them only had mental deficiency, while 272,000 had other organic deficiency. Male:female ratio was 101.4:100. The incidence of 6-14 year-old was 38.14%, which was 3 times higher than that of other age groups. Mild mental deficiency was 16.4 times more than severe mental deficiency. The most common causes were hereditary diseases, encephalitis, malnutrition, cerebral trauma, pregnant diseases, etc. All of the causes belonged to preventable diseases.

Adolescent↗
PubMed1990

[A new serotype of Shigella boydii].

Two strains which belong to the same serotype of Shigella were isolated from the bloody-pus stool of two patients (in 1986) and is reported in this paper. The results were identical both showing agglutination in low titer with serotype 8 of S. dysenteriae and serotype 4 of S. boydii when the two strains were checked well with all kinds of diagnostic antisera and vice versa, ie the antisera produced by the two strains were also checked well with sera prepared with the representative strains of all Shigella spp. No cross agglutination with O6, O7, and O150 of E. coli were found. Consequently, It appears to be a new serotype of Shigella. These two strains possess the ability of causing keratitis in guinea-pigs as well as invading epithelial cells, the DNA of both strains in agarose-electrophoresis showed a large plasmid, indicating that they are virulent strains possessing invasive ability. It was concluded that these two strains belonged to Shigella boydii as they fermented mannitol and non-related antigenically with Shigella flexneri. Since serotype 1-18 of S. boydii have been reported recently, we propose that this new serotype should be serotype 19 of Shigella boydii.

Animals↗
PubMed1990

[Isolation and mapping of arbitrary single copy DNA fragment located on human chromosome 11p11-q11].

A library of genomic DNA has been constructed in EMBL3 lambda phage, from a Chinese hamster/human lymphocytes somatic cell hybrid carrying human chromosome 11 and 20. Recombinants containing human genomic DNA origin can be isolated from the hybrid cell genomic library by using species-specific probe. 8 single copy fragments have been isolated from 13 recombinants. One of them designated as FD11-1 has been identified on chromosome 11 by hybridized it with hybrid cell clone panel and mapped on chromosome 11p11-q11 by chromosome in situ hybridization. On chromosome 11, 3 linkage groups were reported, which located on 11p15, 11p13 and 11q13 respectively. Therefore, the FD11-1 will supply a new locus on chromosome 11 for linkage analysis. Endonuclease recognizing sites and potential recognizing sites on FD11-1 will guide the further RFLP studies.

Chromosome Mapping↗
PubMed1990

[A study on pathogen antigen and serodiagnosis of Malleus and Melioidosis with McAbs].

A study on pathogen antigen and serodiagnosis of Malleus and Mlioidosis was made with McAbs which are against Ps. mallei or Ps. pseudomallei. The results showed that (1) Ps. mallei and Ps. pseudomallei express different surface antigen types respectively, and there are antigen overlapping in some degree between them; (2) Epitope 2D4 is common to Ps. mallei and Ps. pseudomallei; (3) The antibody which is similar to McAbs 4D4 or 1A9 might be a common serum antibody of Malleus and Melioidosis; and (4) McAb 3A1 is a high specific one which reacts with Ps. pseudomallei only, and with it, it is possible to resolve the immunological problem of distinguishing diagnosis between Malleus and Melioidosis.

Animals↗
PubMed1990

[Determination of traces of gallium in air by oscillographic polarography].

Gallium and its compounds in air collected by millipore filter membrane are leached by 10% HNO3 at room temperature. After being adjusted with ammonia solution to pH8-9, the leachate is filtered and the filtrate is evaporated to dryness on a water bath. The residue is dissolved with 2 ml of 10% HNO3 and then into the solution are added 0.5 ml of 5% hydroxylamine hydrochloride solution, 0.20 ml of 5% sulfosalicyclic acid solution, 0.5 ml of NaF solution (1mg F/ml) and 1 drop of methyl red solution. Let it stand for 5 min after the color of the solution turns orange by addition of 1:1 NH4OH solution. Add 0.20ml of 0.20% cupferron solution, 0.20ml of 0.5% diphenyl-guanidine solution (prepared in 95% ethanol) and 0.5ml of acetic acid-ammonium acetate buffer (pH 7.0). Then add water to make the final volume of the test solution to be 10ml and measure the height of the second derivative polarographic wave at -1.08V (vs.SCE). The sensitivity of the method is 0.005-0.10 microgram/ml, the coefficient of variation 6.8%, recovery 97.8%. Within 0.005-0.10 microgram/ml, the wave height is linear with the concentration of gallium.

Air↗