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Biomedical subjects

Z Hussain

Publications and source records attributed to Z Hussain.

At least 127 records · Page 7Linked to original sources
PubMed1987

Comparison of RapID-ANA and Minitek with a conventional method for biochemical identification of anaerobes.

Two micromethods for the identification of anaerobes, one requiring growth (Minitek) and one nongrowth dependent (RapID-ANA), were compared with a conventional identification culture system. For 222 clinical isolates, RapID-ANA agreed with PRAS in 187 (84%) and Minitek agreed for only 170 strains (76%). Both systems identified common isolates well, but encountered some difficulty in identifying less common clostridia and Gram-negative bacilli. Although adequate for most strains, the results from both systems should be interpreted with caution, particularly for less frequently isolated species.

Bacteria, Anaerobic↗
PubMed1987

Comparison of susceptibility results of anaerobic organisms determined by agar dilution method and Sceptor Anaerobe MIC/ID Micro Broth Dilution Panels.

A commercial broth microdilution system (Sceptor Anaerobe MIC/ID, BBL) for susceptibility testing of anaerobic bacteria was compared to a reference agar dilution system. Of the 172 organisms tested, only 7% failed to grow sufficiently for testing in the Sceptor system. In 1,590 antibiotic/organism combinations, 86.7% of the Sceptor results were identical or within one doubling dilution of the reference system. In 91.9% of the cases, interpretation of the results was same in both systems. Two hundred and twelve MIC values, however, differ by greater than or equal to 2 log2 dilution from the reference system. Due to this reduced correlation in the actual MIC values with the reference results, further studies are warranted before the Sceptor system can be recommended for routine use.

Agar↗
PubMed1987

Supplement peptone agar--a simple carbohydrate degradation plate medium for the identification of Neisseria species.

A carbohydrate degradation medium was developed for the detection of acid production by Neisseria species and Branhamella catarrhalis. A total of 223 clinical isolates were identified by Supplemented Peptone Agar and the results were compared with those of Cystine Trypticase Agar. Supplemented Peptone Agar and Cystine Trypticase Agar correctly identified 99.1% and 93.7% of the total strains respectively within 24 h. With Cystine Trypticase Agar method another 4% of the isolates could be identified but required an additional 24 h of incubation.

Culture Media↗
PubMed1987

Endophthalmitis due to Oerskovia xanthineolytica.

Unilateral endophthalmitis developed in a 47-year-old man following an intraocular eye injury with a metallic foreign body. Eventually, Oerskovia xanthineolytica was isolated from the vitreous humour. To our knowledge this is the first documented case of endophthalmitis caused by this organism in humans. The clinical course and management of this opportunistic infection are discussed.

Bacterial Infections↗
PubMed1986

Effect of Cuscuta chinensis water extract on 7,12-dimethylbenz[a]anthracene-induced skin papillomas and carcinomas in mice.

Cuscuta chinensis, known as Aftimun, is reputed to have antitumour activity in the Unani system of medicine in India. The effect of a hot water extract of C. chinensis on 7,12-dimethylbenz[a]anthracene (DMBA)-induced skin papillomas and carcinomas in Swiss albino mice was studied. Oral administration of the extract (1 g/kg body wt) thrice a week in 22 mice, started on the tenth day after the first application of DMBA to the 252nd day, markedly delayed the appearance and retarded the growth of papillomas and the incidence of carcinoma, relative to a control group with 28 mice, in a two-stage system of tumorigenesis. Its prophylactic effect was found to be statistically significant.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1986

Comparison of the MicroScan system with the API Staph-Ident system for species identification of coagulase-negative staphylococci.

To evaluate the accuracy of the MicroScan System (American Hospital Supply Corp., Sacramento, Calif.) for identification of coagulase-negative staphylococci, we tested 175 clinical isolates of coagulase-negative staphylococci. The results obtained by the MicroScan system were compared with those of the API Staph-Ident system (Analytab Products, Plainview, N.Y.). Forty-three discrepancies between the two systems were resolved by the conventional method of Kloos and Schleifer (W.E. Kloos and K.H. Schleifer, J. Clin. Microbiol. 1:82-88, 1975). The MicroScan and the Staph-Ident systems correctly identified 146 (86.4%) and 154 (88%) of 175 strains, respectively. The API system failed to identify phosphatase-negative Staphylococcus epidermidis. The MicroScan system demonstrated the greatest accuracy in the identification of S. epidermidis and S. saprophyticus, whereas lesser accuracy was achieved with S. hominis, S. warneri, and S. sciuri.

Bacteriological Techniques↗
PubMed1985

Streptococcus pyogenes as a cause of nosocomial infection in a critical care unit.

A cluster of five cases of Streptococcus pyogenes infection or colonization was identified in an adult critical care unit following the admission of a patient with a severe cutaneous infection. This report deals with the nature of the outbreak and methods of control, and comments on infections of this nature in adult critical care areas.

Adult↗
PubMed1985

Efficacy of tetroxoprim/sulphadiazine in the treatment of Pneumocystis carinii pneumonitis in rats.

Pneumocystis carinii pneumonitis was induced using dexamethasone in male Sprague-Dawley rats. After the first death due to Pn. carinii occurred, nine rats received 50 mg/kg/day tetroxoprim and 120 mg/kg/day sulphadiazine. Four additional rats were given no therapy and served as a positive control. All the surviving rats including five negative controls were sacrificed four weeks after the last positive control rat died. All four rats in the positive control group and two of nine in the treated group developed Pn. carinii pneumonitis, whereas none of the five negative controls had evidence of Pn. carinii infection. The difference between the treated and untreated rats was significant (P = 0.05). These results suggest that combination therapy with tetroxoprim/sulphadiazine is effective in the treatment of Pn. carinii pneumonitis in this animal model.

Animals↗
PubMed1985

Renal stones in children in Pakistan.

One hundred and fifty children up to the age of 12 years with documented evidence of renal or ureteric stones were studied between July 1973 and June 1983. The peak age group was between 10 and 12 years, the male to female ratio being 1.9:1. Abdominal pain was the commonest presenting symptom (66.6%). Asymptomatic stones or vague symptoms in 23 patients warrant a higher index of suspicion of renal stone disease in children. Thirty patients had associated renal insufficiency. One third of the children had a positive urine culture, Proteus spp. being the commonest organism. Congenital anomalies of the urinary tract were seen in 12% of patients. In the majority (59%), aetiological factors related to stone formation could not be identified. One hundred and thirty patients underwent surgery for removal of stones. Analysis of calculi by X-ray diffraction and infrared spectroscopy revealed that stones were predominantly of mixed type, calcium oxalate being the commonest compound. The overall recurrence rate following surgical removal was 15%.

Age Factors↗
PubMed1985

Adaptation of a coculture technique to the Minitek anaerobe system.

A method to produce anaerobic conditions by coculture with a nonfermentative organism was utilized in conjunction with the Minitek anaerobe system (BBL Microbiology Systems, Cockeysville, Md.) for identification of anaerobic bacteria from clinical specimens. With the coculture method, the Minitek anaerobe identification tests could be incubated under aerobic conditions. In 1,900 individual biochemical reactions, 1,826 (96%) were identical whether anaerobic conditions were achieved by conventional or coculture techniques. In comparison with the reference identification (Virginia Polytechnic Institute and State University, Blacksburg), both systems of incubation identified 91 of 99 strains (92%) correctly. The method of incubation had an effect on identification to the genus level in 1 of 99 (1%) strains and to the species level in 3 of 99 (3%) strains.

Bacteria, Anaerobic↗
PubMed1985

Comparison of American MicroScan dry and frozen microdilution trays.

This study compared the American MicroScan frozen microdilution tray and dehydrated microdilution tray. A total of 153 gram-negative and 106 gram-positive isolates were tested concurrently on both tray types and read visually. Very major and major antimicrobial susceptibility discrepancies amounted to 1.0% of all results, and minor discrepancies amounted to 0.4% of all results. The biochemical discrepancy rate for gram-negative organisms was 1.3%, resulting in no identification errors either at the genus or the species level. Gram-positive identification was not evaluated, as the formulation of biochemicals was changed after this evaluation was completed. Overall, the results with the dry tray were acceptable, providing a suitable alternative to the frozen tray.

Anti-Bacterial Agents↗
PubMed1984

A new approach for presumptive identification of clinically important streptococci.

Several tests, bacitracin, CAMP, pyroglutamic acid-B-naphthylamide test (PYR test), bile aesculin hydrolysis, salt tolerance and pigment production tests were evaluated for their reliability and speed in presumptive identification of streptococci. Bacitracin correctly identified all of the group A streptococci but 5% of other beta haemolytic streptococci were misidentified as group A. The PYR reaction was just as sensitive but more specific for group A streptococci and the results were available within 2-3 h. The PYR reaction also effectively differentiated between enterococcal and non-enterococcal group D streptococci. Both the salt tolerance test and the PYR reaction misidentified bile aesculin positive non-group D streptococci as non-enterococcal group D streptococci. Pigment production by group B streptococci was more reliable than the CAMP test for identifying group B streptococci. The combination of PYR, bile aesculin and pigment production allowed rapid differentiation of several medically important groups of streptococci.

Bacitracin↗
PubMed1984

Development and evaluation of a coculture technique for identification of anaerobic organisms.

We investigated a method to reduce oxygen tension and produce anaerobic conditions conveniently by coculture with a nonfermentative organism. The biochemical reactions of 94 strains representing 41 different species were determined using prereduced media and gas chromatography according to standard methods. The standard reactions were compared with those obtained by preparing similar media, modified by containing no cysteine or resazurin, under aerobic conditions. Preincubation of the modified biochemical media with a standard suspension of a culture of Acinetobacter lwoffi provided reducing conditions. In 1,449 individual tests using 22 different biochemicals, 1,349 (93%) of the reactions agreed. Only two misidentifications resulted when using the modified media, one each at the species and genus levels. Two strains of Actinomyces odontolyticus failed to grow in coculture.

Acinetobacter↗