An improved preparation for determination of bradykinin.
The cat isolated jejunum kept either in an organ-bath or under superfusion can be rendered extremely sensitive to bradykinin after exposure to chymotrypsin. Treated preparations responded to as little as 100 pg/ml of the peptide. Use has been made of sensitized preparations to determine bradykinin-like material of dog blood extracts obtained in experimental "dumping syndrome".
Acetylcholine↗