Biomedical subjects

Y Wu

Publications and source records attributed to Y Wu.

At least 433 records · Page 24Linked to original sources

PubMed2000

[Exploration of factors increasing transfer efficiency of retroviral mediated multidrug resistance gene (mdr1) into human CD(34)(+) cells].

OBJECTIVE: To explore factors influencing transfer of retroviral mediated multidrug resistance gene (mdr1) into human CD(34)(+) cells. METHODS: Transduction efficiency in the presence of different combinations of cytokines and human bone marrow stromal cells plus cytokines were determined by flow cytometry (FCM). Drug resistance was evaluated by plating yields of cultured hematopoietic progenitor cells. The effect of Taxol at different concentrations on mdr1 gene transfer cells was determined by FCM. RESULT: Transduction efficiency in the presence of SCF + FL + IL-3 was higher than that in other combinations of cytokines (SCF + IL-6 + IL-3, SCF + IL-6 + IL-3 + Tpo, SCF + IL-3). Transduction efficiency (20.5%) in the presence of bone marrow stromal cells plus cytokines (SCF + FL + IL-3) was higher than that (15.2%) without stromal cells, and the number of drug-resistant colony-forming cells was more in the former than in the latter. The percentage of CD(34)(+) cell with the gene transduction at 10 ng/ml Taxol reached 38.5%. CONCLUSION: Human bone marrow stromal cells plus cytokines (SCF + FL + IL-3) is more effective in enhancing mdr1 gene transduction. Taxol at a certain concentration can enrich mdr1 gene transferred cells.

Antigens, CD34↗

PubMed2000

[Protection of hematopoietic stem cells by MIP-1alpha and PF4 against the cytotoxicity of chemotherapeutic agents].

OBJECTIVE: To study the protective effects of macrophage inflammatory protein-1alpha (MIP-1alpha) and platelet factor 4 (PF4), alone and in combination, on hematopoietic stem/progenitor cells against the cytotoxicity of chemotherapeutic drugs. METHODS: Bone marrow and cord blood mononuclear cells (BMMNC and CBMNC) and HL-60 cells were pretreated with MIP-1alpha, PF4, MIP-1alpha + PF4, and PBS respectively for 48 hours, and then incubated with DNR for an additional 24 hours. Cell viability, cell cycle, CD(34)(+) CD(38)(-) cells, colony forming units (CFU) and protein expression of p16, p27 gene were measured. RESULTS: (1) Cell viability, the number of CD(34)(+) CD(38)(-) cells and CFU yields of BMMNC and CBMNC in MIP-1alpha and PF4 groups were significantly higher than that in control groups (P < 0.05). (2) Cells in S + G(2) phase in MIP-1alpha and PF4 groups were significantly fewer than that in control groups (P < 0.05). (3) MIP-1alpha upregulated the expression of p16 gene of stem/progenitor cells. PF4 showed no effects on expression of both p16 and p27 genes. (4) Hematopoietic protection of MIP-1alpha was stronger than that of PF4. No cooperative effect could be seen in combination of the two agents. (5) Cell viability, cell cycle and expression of p16 and p27 gene of HL-60 cells were not affected by either MIP-1alpha or PF4. CONCLUSION: MIP-1alpha and PF4 can reversibly and selectively protect hematopoietic stem/progenitor cells against cytotoxicity of chemotherapeutic agents. MIP-1alpha can suppress cell proliferation by upregulating the expression of p16 gene and block the cell cycle at G(0) phase, resulting in the elevation of cell resistance to chemotherapeutic drugs.

ADP-ribosyl Cyclase↗

PubMed2000

[Preventive effect of cardiomyopeptidin on rat heart injured by ischemia-reperfusion].

OBJECTIVE: To investigate the preventive effect of cardiomyopeptidin of small molecular weight polypeptide on rat hearts injured by ischemia-reperfusion. METHODS: In a rat model injured by ischemia-reperfusion in the heart, observation was made on the influence of cardiomyopeptidin on the activities of creatine kinase (CK) and lactate dehydrogenase(LDH), as well as on the content of MDA in plasma after the preventive drug was used. RESULTS: Cardiomyopeptidin could obviously prevent the injury caused by ischemia-reperfusion, reduce the activities of CK and LDH and the content of MDA in a dose-dependent manner. CONCLUSION: Cardiomyopeptidin has a preventive effect on myocardium injured by ischemia-reperfusion and this may be related to its reducing the release of myocardial enzyme and anti-lipoperoxidation.

Animals↗

PubMed2000

[Analysis on 2158 neural tube defects diagnosed prenatally by ultrasound examination].

A retrospective survey was made to study the prenatal diagnosis on neural tube defects(NTD) by ultrasound examination from 1990 to 1993 in China. A total of 2158 livebirths and stillbirths affected by NTD with 28 weeks or more of gestation were investigated in 233 hospitals of 28 provinces, municipalities and autonomous regions all over the country. The results showed that 61.8% of the NTD were diagnosed prenatally, and 56.5% of the NTD were diagnosed by ultrasound examination. The ratios of anencephaly, myelocele and meningocele diagnosed prenatally by ultrasound examination were 66.7%, 62.5% and 52.5% respectively. Thoracic(46.4%) and lumbar (43.4%) spina bifida were diagnosed more frequently than cervical(35.5%) and sacral(31.7%) spina bifida. The ratios of prenatal diagnosis on NTD by ultrasound examination in provincial, municipal, county, and factory hospital units were 55.7%, 59.4%, 49.0% and 57.2% respectively. The ratios of prenatal diagnosis of NTD by ultrasound examination from 1990 to 1993 were 53.5%, 55.8%, 60.7% and 54.7% respectively. There was an increase in the prenatal diagnosis of NTD by ultrasound examination from 1990 to 1993. The ratios of prenatal diagnosis of NTD in county hospitals by ultrasound examination were relatively low. Anencephaly, myelocele and meningocele were more frequently diagnosed by prenatal ultrasound examination.

Adult↗

PubMed2000

[Evaluation on usage of serum troponin-I, CK-MBmass and myoglobin measurements in diagnosing acute myocardial infarction].

The purpose of this study was to search the sensitivity of three indicators in early diagnosis of acute myocardial infarction (AMI) and analyze their window-time duration in serum, along with the decision on experimental golden indicator in later AMI inspection and effect assessment after recovery by testing the concentrations of CTnI, CK-MBmass and Mb in AMI patients' serum. By using corpuscle chemiluminescence, the serum concentrations of CTnI, CK-MBmass and Mb in AMI patients, UA (unstable angina) patients, SM (skeletal muscle) patients and a normal group were assayed and compared. A dynamic analysis was followed in five periods, which separately focused on 3-5, 8-14, 20-24, 72 hours and 7 days after heart aching occurred in AMI patients. The results showed the positive rates of CTnI, CK-MBmass and Mb in AMI patients were 65%, 70% and 70% respectively, and the positive rate of CTnI in UA and SM groups was 0%. The CTnI, CK-MBmass and Mb values in group AMI were significantly higher than those in groups of UA and SM. Separately, the CTnI value in AMI was 84 and 91.63 times (the CTnI values) in UA and SM; the CK-MBmass value in AMI was 8.82 and 4.67 times and the Mb value in AMI was 11.57 and 2.25 times as high as those in UA and SM. Significant difference was observed by the comparison between AMI group and UA, SM groups (P < 0.001). According to the dynamic analysis, CTnI, CK-MBmass and Mb rose significantly at the early stage of AMI onset (3-5 hrs), and then gradually increased with time. The climax occurred in the period of 8-14 hrs and Mb returned to its normal state in 72 hrs. On the 7th day, CTnI still existed in serum, but CK-MBmass approximated to normality. In conclusion, these data demonstrate a relatively high sensitivity of CTnI, CK-MBmass and Mb in early diagnosis of AMI. CTnI is specific to cardiac muscle for its special positive rate. Also, CK-MBmass and Mb are of higher clinical value in diagnosing cardiac muscle damage. CTnI has the longest window-time, which makes it the best special indicator for terminal inspection and recovery decision on AMI.

Adult↗

PubMed2000

[Value of combined detection of tumor markers for the prediction of small cell and non-small cell lung cancer].

To evaluate the value of detection of 4 tumor markers(CEA, CA125, gastrin, and NSE) for histological types in patients with lung cancer and to improve the predicted efficiency of tumor markers for distinguishing between small cell lung cancer(SCLC) and non-small cell lung cancer (NSCLC), these 4 tumor markers in serum were determined in 51 patients (21 cases with SCLC, 30 cases with NSCLC) with confirmed primary diagnosis of lung cancer of different histology by radioimmunoassay. Linear learning machine method, PRIMA method and KNN method were used to classify SCLC and NSCLC. The levels of gastrin and NSE in SCLC were apparently higher than those of gastrin and NSE in NSCLC, but the levels of CEA and CA125 in SCLC were significantly lower than those in NSCLC. Smoking had an effect on the levels of CEA and CA125, but had little effect on those of gastrin and NSE. The total accuracy of the three methods was over 85% in distinguishing SCLC from NSCLC. So combined detection of the four tumor markers in serum might be useful in the prediction of histological types in patients with lung cancer.

Adenocarcinoma↗

PubMed2000

[Hygienic status of deep fried oils in 1998 and the contrast with 10 years ago].

The hygienic status and influence factors of deep fried oil were studied. The samples from 29 individual producers, 38 state-operated or cooperated restaurants and 30 joint-invested enterprises were collected to investigate the hygienic condition on the spot, and to analyze the acid value(AV), carbonyl value (COV) and polar compound(PC) with national standard methods(GB). The data showed that the degree of rancidity of the deep fried oils varied with different types of oil, refinement of oil and the kind of fried food. Comparing with the results obtained a decade ago, COV was decreased while PC was increased. It was suggested: (1) to specify some types of oil for flying foods; (2) to develop some quick methods for checking fried oil; (3) to improve the method for detecting PC for solid oil at ordinary (indoor) temperature; (4) to study the quality and safety of fried foods prepared by solid oils.

Acids↗

PubMed2000

[Optimal treatment parameters for damage of chicken comb vessels using chlorophyll derivative IV in photodynamic therapy].

OBJECTIVE: To define the optimal treatment parameters of chlorophyll derivative IV (CPD4) in photodynamic therapy(PDT) of port wine stains(PWS) by observing the morphological changes of chicken combs. METHODS: 102 animals were divided into 6 groups at random, including 3 control groups(given no treatment, given light without photosensitizer or given photosensitizer without light) and 3 experimental groups treated with different CPD4 dosages(5, 7.5, 10, 12.5, 15 mg/kg body weight), power density(50, 100, 150, 200 mW/cm2), energy density (15, 30, 45, 60, 120, 135, 180 J/cm2) and different intervals between CPD4 injection and irradiation (immediately, 5, 10, 20, 30, 60, 120, 180, 240 minutes). The main emitting peak of light source was at 650 nm, and CPD4 was injected intravenously, then the appearance of chicken comb was recorded everyday. The prepared sections were stained with HE. The histological changes of chicken comb were observed by light microscope. RESULTS: The control animals showed no significant comb changes, while the PDT could cause blanching of the chicken comb in the experimental group. The histologic changes included damage of erythrocytes and endothelial cells, vascular rupture, hemorrhage or disappearance, denaturation of perivascular collagen fiber and local necrosis of chicken combs. The treatment parameters which caused the most obvious blanching of the chicken comb were 7.5-10 mg/kg body weight of CPD4 dosage, 100-150 mW/cm2 of power density, 60-90 J/cm2 of energy density, and the intervals between CPD4 injection and irradiation were 0-20 minutes. CONCLUSION: CPD4 in PDT can cause selective damage of dermal capillaries in chicken comb and blanching of the chicken comb with optimal treatment parameters. The results will offer the basis for further investigation of clinic treatment parameters.

Animals↗

PubMed2000

[Survey of the patients with oblique facial clefts in China].

OBJECTIVE: A descriptive study was conducted to investigate the epidemiological characteristics of oblique facial clefts in China. METHODS: From 1987 to 1992, data were collected from more than 500 hospital-based units involved in a monitoring program and were analyzed by the National Center for Birth Defects Monitoring. The monitored subjects were the hospitalized births from 28 weeks gestation to a period within 7 days after delivery. RESULTS: There were 71 cases of oblique facial clefts in 4,489,692 births with an incidence of 0.16/10(4) in China. The incidence of oblique facial clefts in the rural and urban areas were 0.19/10(4) and 0.14/10(4) respectively, 0.18/10(4) in males and 0.12/10(4) in females. No significant differences were found. The unilateral oblique facial clefts were counted for 80%, and bilateral cases 20%. The incidence of unilateral oblique facial clefts in males is higher than that in females. The perinatal mortality was as high as 51%. There are 76% cases with associated abnormalities, most of which involved the maxillofacial region. The most common associated abnormality is cleft lip with cleft palate. CONCLUSION: The incidence of oblique facial clefts in China is the same as those in other countries. There is an unilateral predominance in this defect, especially in males. The perinatal mortality of this defect is high.

China↗

PubMed2000

[The molecular mechanism of neuronal migration].

A large amount of work since the late 1800 have shown that, during embryonic and postnatal development, the majority, if not all, neurons in mammalian nervous system have to migrate a certain distance to reach their final destination. An interesting question is how neurons are guided for their migration. We are interested in the molecular mechanisms underlying neuronal migration and our work published in 1999 indicates that there are diffusible molecules in the brain which can guide the direction of migrating neurons; specifically, a secreted protein called Slit is repulsive to neurons and its concentration gradient guides neuronal migration.

Aged↗

PubMed2000

[Nonsyndromic cleft lip with or without cleft palate in Chinese population: analysis of 3766 cases].

A retrospective study was conducted to evaluate the variation trend in the incidence and epidemiological characteristics of nonsyndromic cleft lip with or without cleft palate in Chinese population. From 1988 to 1992, data were collected from more than 500 hospital-based units involved in a monitoring program and were analyzed by the National Center for Birth Defects Monitoring. The monitored subjects were the hospitalized births from 28 weeks gestation to a period within 7 days after delivery, which included 3246 408 perinatals and 3766 cases. The total incidence of nonsyndromic cleft lip with or without cleft palate was 11.6/10,000; the total incidence of celft lip alone was 3.8/10,000 and that of cleft lip with cleft palate was 7.8/10,000; no significant variation was noted in the annual incidence from 1988 to 1992. The incidence in males was 13.3/10,000, and 9.5/10,000 in females; there was a significant difference (P < 0.01). Of all the 3766 cases, cleft lip with cleft palate accounted for 67%, unilateral cleft lip 28% and unilateral cleft lip with cleft palate 43%. Left cleft lip and left cleft lip with cleft palate constituted 21% and 29% respectively. The sex-ratio was 1.6:1 for nonsyndromic cleft lip with or without cleft palate. It could be concluded that the incidence of nonsyndromic cleft lip with or without cleft palate showed no variation trend in China. Although no difference was found between urban and rural areas, the susceptibility to the defect was higher in males than in females. Cleft lip with cleft palate was more common than other types. There was a predominance of unilateral defects, of which most were on the left side.

China↗

PubMed2000

[Biomachining of metal copper by Thiobacillus ferrooxidans].

Thiobacillus ferrooxidans was employed in the biomachining process of metal copper(Cu0). The bacteria growth and the changes of Fe3+ concentration during machining processes have been studied. Biomachining and chemical machining have been compared. The results showed that the concentrations of bacteria and Fe3+ determine the speed of machining copper. The biomachining is more fast that chemical maching because bacteria are able to regenerate Fe3+ oxidizing copper. It was also found that the Cu2+ produced from the machining processes inhibit the growth of bacteria. Cu2+ has to be removed.

Copper↗

PubMed2000

[Determination of naringin and synephrine in fructus aurantii from different habitats by HPLC].

This paper reports the determination of Naringin and Synephrine in Fructus Aurantii from different habitats by HPLC. The result shows the highest content of Naringin in Citrus aurantium L. 'Xiangcheng' from genuine crude drug habitat Xingan city Jiangxi provice, the highest content of synephrine in Citrus aurantium L. 'Xiangcheng' from Yiyang city Jiangxi provice. Quality evaluation of genuine crude drug was studied preliminarily.

China↗

PubMed2000

[GC-MS analysis of essential oil components of Alpinia oxphylla].

Essential oil Components of Alpinia oxphylla were analyzed by way of applying gas chromatography, mass spetrography and computer interpretation; essential oil was abtained by supercritical CO2 fluid, thus we got 193 peaks in oil from Pot I and 210 peaks in oil from Pot II as well as identify 139 and 155 compounds respectively.

Alpinia↗

PubMed2000

[The muscarinic receptor subtype study of the action on lowering IOP of cholinomimetic drugs].

PURPOSE: Clarify the relationship between lowering intraocular pressure (IOP) action of cholinomimetic drugs and muscarinic receptor subtypes. METHODS: Measuring rabbit IOP with gas driving ophthalmotonometer, measuring size of pupil with pupil rule and measuring contraction of rabbit iris by iris experiment in vitro. Figuring out pD2 of cholinomimetic drugs and pA2 of the antagonists of muscarinic receptor subtypes. RESULTS: The order of effect on lowering IOP and miosis of cholinomimetic drugs is Erycibele Alkaloid > Aceclidine > Pilocarpine and the order of effect on constracting iris is Erycibele Alkaloid > Pilocarpine > Aceclidine. The sequence of antagonistic effect of subtype antagonists is 4-DAMP(M3) > Pirenzepine(M1) > Gallamine(M2). CONCLUSION: Effect cholinomimetics on lowing IOP and miosis is primary mediated by M3 receptor, next by M1 receptor and has little relation with M2 receptor.

Animals↗

PubMed2000

[Ocular axial length and refractive changes in pediatric pseudophakia].

PURPOSE: To evaluate the ocular axial length and refractive changes that occured in children who had cataract extraction with intraocular lens implantation and to investigate the factors that influenced the pseudophakic refraction. METHODS: A review of 12 eyes in 10 children (mean age 7.16 years, range 3.83 to 10.16 years) who had cataract extraction with intraocular lens implantation was undertaken. Patients were followed for an average of 35.8 months. The preoperative and the last postoperative axial length and corneal curvature (K readings) in both operated and unoperated eyes were measured. The distance from the vertex of the cornea to the anterior vertex of intraocular lens (AVpc) was measured in the final follw-up. The initial (in two weeks) and the last refractive status of pseudophakia were examined. Overall age at surgery averaged 7.16 years (range 3.83 to 10.16 years), with followed up of 35.8 months. RESULTS: In the 12 operated eyes, the mean axial growth was 0.39 mm, whereas in the other eight unoperated eyes it was 0.66 mm. Though the axial length of both eyes increased significantly after surgery (P < 0.05), there was no significant difference in the postoperative increase of axial length between the two groups(P > 0.05). In K readings, there was no significant difference between the operated and unoperated eyes before surgery and in the last follow-up respectively. There was no significant difference between the preoperation and the last follow-up in the operated and the unoperated eyes respectively. The average calculated AVpc (AVpc2) was 4.66 30 mm, but the average objective AVpc (AVpc1) measured with ultrasonic biometry was 3.83 mm. The average difference between them was 0.8320 mm and was significant (P < 0.01). According to SRK formula, using preoperative and the last postoperative axial length and corneal curvature to predict refractive changes, the myopic shift was -1.53 D, but the average objective refractive difference between the last and initial examination after surgery was -3.86 D. The average difference between the predicted and actual postoperative refractive changes was significant (P < 0.05). CONCLUSION: Our study suggests that there may be no effect on ocular growth in children (3 to 10 years) followed cataract extraction and intraocular lens implantation. Increasing of axial length and moving forward of intraocular lens induced by complication may result in myopic shift in pseudophakia of children. Slightly undercorrected eyes with intraocular lens in children after cataract extraction will gradually move to emmetropia or moderate myopia in adulthood.

Adolescent↗

PubMed2000

[Pedicled galeal flap in the reconstruction of head and neck tumor defects].

OBJECTIVE: To evaluate the advantage and applications of pedicled galeal flap in head and neck region. METHODS: A consecutive series of 17 patients underwent surgical reconstruction with pedicled galeal flaps after head and neck tumor resection. The defects included nasopharynx, skull base, maxilla, orbital base, oropharynx and oral cavity and the size ranged from 5 cm x 5 cm to 10 cm x 10 cm. The technique for using this flap was described and application was illustrated with 3 case reports. RESULTS: Complete success of galeal flaps for the reconstruction of head and neck defects was achieved in 13 of the 17 cases (76.5%) and partial necrosis was observed in the remaining 4 cases (23.4%). Immediate wound complications occurred in four cases, which resolved spontaneously. Four delayed complications were observed in 4 of 9 survival cases that included trismus (3) and alopecia (1). CONCLUSION: Galeal flap is a thin, pliable and well vascularised reconstruction tissue and is highly reliable. The donor site morbidity is minor. We have found the flap to be useful in the reconstruction of a variety of defects in head and neck, especially in skull base, orbital base, nasopharynx and oropharynx.

Adolescent↗

PubMed2000

[Preoperative superselective intra-internal iliac arterial chemotherapy for invasive bladder cancer and curative analysis].

OBJECTIVE: To study clinical effects of preoperative intra-internal iliac-arterial chemotherapy for invasive bladder cancer. METHODS: 14 cases of invasive bladder cancer underwent superselective intra-internal iliac-arterial chemotherapy before conservative cystectomy. RESULTS: After 2-3 week therapy, reduction of cancer volume (20%-50%), clotted by thrombus in the blood vessels of the cancer or circumferential tissue, and decreased bleeding were found. Pathomorphological features included large patchy necrosis, degeneration, and inflammatory changes of carcinoma tissue. Follow-up ranged from 5 to 42 months (mean 19.6 months). One T3 N1 M0) of the cases (0.7%) relapsed into T1N0M0 and the others did not. CONCLUSIONS: The intra-internal iliac-arterial method, simple, safe, coordinates in pre-operation for invasive bladder cancer, and increases the operative opportunity for preservation of functional bladder.

Aged↗