Biomedical subjects

Y Wu

Publications and source records attributed to Y Wu.

At least 415 records · Page 23Linked to original sources

PubMed2000

[Functional reconstruction of the hand after electrical injury with a composite flap of the vessel nerve and fascia].

OBJECTIVE: In the case of electrical injury of the wrist, it may be necessary to transplant a long tendon, nerve and vessel for functional reconstruction. But the fibrosis of the recipient bed often makes the survival and functioning of the grafts difficult. This clinical study was to solve the problem. METHODS: A composite flap was designed consisting of the small saphenous vein, the sural nerve and the fascia of the lower-leg. The small saphenous vein and the nerve were transplanted to repair the defects of the radial or ulnar artery and the media nerve. The fascia was used to reconstruct the recipient bed for tendon grafting. RESULTS: Six cases were treated with this method with success in 5 cases. 5 to 18 months' follow-up revealed restoration of sensation and finger flexion in the 5 cases. CONCLUSION: This method is effective in alleviating tendon adhesion, accelerating functional restoration of the injured hand.

Adolescent↗

PubMed2000

Nociceptin inhibits electric field stimulation-induced cholinergic constrictions in rat airways.

AIM: To study the effect of nociceptin (orphanin FQ), a newly discovered heptadecapeptide, on cholinergic constrictions in isolated trachea and bronchus of rat. METHODS: The electric field stimulation (EFS) induced a monophasic constriction, which was due to an activation of the cholinergic nerves. RESULTS: Nociceptin 0.001-0.1 mumol/L inhibited cholinergic constriction in a concentration-dependent manner. IC50 (95% of confidence limits) were 0.06 (0.04-0.08) mumol/L and 0.07 (0.05-0.1) mumol/L in tracheae and bronchi respectively. The constrictions inhibited by nociceptin 0.01 mumol/L in traheae and bronchi were (58 +/- 32)% and (60 +/- 26)% respectively compared with the control, in which nociceptin was not applied. After pretreatment with naloxone 0.1 mumol/L, the constrictions were (60 +/- 19)% and (54 +/- 20)% (P > 0.05 vs the above figures). However, the constrictions induced by exogenous acetylcholine were unaffected by nociceptin 0.01 mumol/L. kappa-Opioid receptor agonist, U-50488H (0.01-1 mumol/L) did not affect the EFS-induced cholinergic constrictions in rat airways. CONCLUSION: Nociceptin inhibits EFS-induced cholinergic constriction, which is not affected by naloxone in rat airways.

3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cycloh↗

PubMed2000

Effects of angiotensin II on release of CRH and AVP from hypothalamus during acute hypoxia.

AIM: To investigate the effects of angiotensin II (Ang II) on corticotropin-releasing hormone (CRH) and argipressin (AVP) release from median eminence (ME) of hypothalamus during acute hypoxia in rats. METHODS: Simulated hypoxia was performed in a hypobaric chamber. CRH and AVP were determined by radioimmunoassay (RIA). Plasma corticosterone concentration was measured by fluorometry. RESULTS: Ang II did not influence CRH release induced by hypoxia [CRH in group pretreated with Ang II (16 +/- 8) ng/ME vs control (15 +/- 4) ng/ME, both exposed to hypoxia with simulated altitude 7 km (8.2% O2)]. Ang II enhanced AVP release, from (5.7 +/- 1.6) ng/ME in control decreasing to (2.6 +/- 1.2) ng/ME (P < 0.05), meanwhile plasma corticosterone concentration was also increased markedly, from (356 +/- 58) in control to (536 +/- 134) micrograms/L plasma (P < 0.05), which was partly abolished by administration of AVP antiserum. CONCLUSION: Ang II might stimulate hypothalamo-pituitary-adrenal axis (HPA) through activating AVP but not CRH release during acute hypoxia.

Angiotensin II↗

PubMed2000

[Optimal models on sustainable management of oases ecosystem in southern margin of Taklamakan Desert].

On the basis of analyzing the distribution feature of water resource and the canal water utilization coefficient of oases in southern margin of Taklamakan Desert, observing the wind prevention efficiency of shelterbelt through a simulation experiment in wind tunnel, and 15 years researching the comprehensive control of desertified land in Cele Oasis, a series of optimal models on sustainable management of oases ecosystem is southern margin of Taklamakan Desert were proposed i.e., the optimal model on "moderated osais", the optimal model on structure of wind-breaks, the optimal model on comprehensive control of desertified land, and the optimal model on planting structure of corps.

Agriculture↗

PubMed2000

[Determination of tannin in cotton plant].

Three methods were used to determine the tannins content in cotton plant tssues. The tannin content in different organs and terminal leaves of four cotton varieties at different growth stages determined by the Folin's assay was nearly two folds as much as that determined by acid butanol assay, indicating that the Folin's assay only offers the total phenol content, but is not suitable for determining the content of condensed tannin in cotton. There was no significant difference between the results of vanillin assay and acid butanol assay, and hence, it is feasible to use vanillin assay to test the tannin content in cotton. Determinations at boll stage showed that the content of condensed tannin in calyx, boll shell and leaf was rather high, generally up to 5-10%, while that in petal, stigma, ovary and inner boll was lower (about 2%). The condensed tannin content in terminal leaf continually increased from seedling(< 1%) to boll stage (10% or so), indicating that the increased tannin content is closely related to the maturity, decrepitude and lignification of cotton tissues.

Gossypium↗

PubMed2000

[Chemoprotection of transfer of multidrug resistance gene into human hematopoietic progenitor cell].

OBJECTIVE: To observe the effect of the transfer of multidrug resistance gene (mdr1) into human hematopoietic progenitor cells (HPC) on the chemoprotection. METHODS: Human CD34+ cells served as a target of mdr1 gene transfer. Retroviral vector SF-mdr containing human total length mdr1cDNA was introduced into packing cells GP-envAM12 by liposome-mediated transfection. The mdr1 gene was transduced into human CD34+ cells by retroviral supernatants of packing cells. The integration and expression of the mdr1 gene and its protein (P170) in transduced cells were determined by PCR, RT-PCR, and flow cytometry. The drug resistance of chemotherapy in transduced HPC was determined by culturing colonies. RESULTS: The mdr1 gene was integrated and expressed in transduced CD34+ cells. The efficiency of mdr1 gene transfer was 10%-14%. Compared with untransduced controls, within a certain range of drug concentration, the number of drug-resistant colony in transduced HPC for taxol, doxorubicin, VCR and VP16 were increased by 3.6 +/- 2.1 fold, 2.9 +/- 0.3 fold, 1.9 +/- 0.4 fold, and 3.5 +/- 0.5 fold, respectively. CONCLUSION: The transfer of the mdr1 gene into human HPC can increase the drug resistance of the transduced cells to corresponding chemotherapeutic drugs that may provide some degree of chemoprotection for HPC.

Antigens, CD34↗

PubMed2000

[Effects of estrogen on serum lipids, plasma endothelin-1, atherosclerosis in ovariectomized rabbits].

OBJECTIVE: To investigate the effects of estrogen on serum lipids, plasma endothelin-1(ET-1) and atherosclerosis in ovariectomized(OVX) rabbits. METHODS: Forty-five female rabbits were randomly divided into 5 groups, there are 9 in each: group A: normal control; group B: sham operation + cholesterol feeding; group C: OVX + cholesterol feeding; group D: OVX + cholesterol feeding + estradiol benzoate; group E: OVX + cholesterol feeding + estradiol valerate. The OVX rabbits fed with cholesterol were used as a model for atherosclerosis. After fed for 12 weeks, all rabbits were sacrificed, and the levels of serum lipids, plasma ET-1, atherosclerosis plaque of aorta were measured. RESULTS: (1) Similar with group B, the total cholesterol (TC) of group D and E [(24.95 +/- 5.31) mmol/L and (27.53 +/- 13.18) mmol/L respectively], were significantly decreased as compared with group C which was (37.69 +/- 4.16) mmol/L. (2) The plasma ET-1 level of group C, which was (343.37 +/- 106.10) ng/L, was significantly higher as compared with group D and E, which were (262.19 +/- 36.45) ng/L [symbol: see text] (245.37 +/- 35.76) ng/L respectively. (3) There were no statistically differences of lipidic sedimentation area among group D, E, and B, which were (17.30 +/- 5.44)% and (36.99 +/- 20.29)% and (29.03 +/- 9.67)% respectively. All of them were significantly decreased as compared with group C, which was (74.83 +/- 12.58)%. CONCLUSION: Estrogen may improve the serum lipids, decrease the plasma ET-1 level, and prevent the atherosclerosis of ovariectomized rabbits.

Animals↗

PubMed2000

[The study of nitric oxide synthase in ovary tumor].

OBJECTIVE: To study the activity of nitric oxide synthase (NOS) in ovarian tumors. METHODS: Spectrophotometry was used to examine NOS activity and NADPH-diaphorase histochemistry to identify its localization. RESULTS: The activity of NOS was significantly higher in malignant than in normal ovarian tissue and benign ovarian tumors (P < 0.01). There was negative correlation between NOS activity and tumor cell differentiation. NOS reaction product was mainly located in cancer cells, and was negative in the interstitial tissue. Furthermore, NOS was mostly located in membrane and cytoplasm of the cancer cell. CONCLUSION: Increase in nitric oxide synthase activity might be related to the growth and malignant behavior of ovarian cancer.

Adolescent↗

PubMed2000

[Geometric relationship of axial crown contour and gingiva contour of normal second mandibular premolars].

OBJECTIVE: To investigate the relationship of axial crown contour and surrounding healthy gingiva contour. METHODS: Mandibular second premolars were selected as the object of study. Three cross-sections were made through crown and gingiva of the model tooth. Curve functions of axial buccal crown and gingiva contour of each section were acquired by means of curve fitting, and the angle of emergence profile was calculated, which represents the degree of undercut. RESULTS: There is unique relationship between axial crown and gingiva contour individually. This character of tooth is similar to its contralateral tooth. The emergence profile angles are in the range of 40 degrees-110 degrees, 84.1% of the angles are in range of 70 degrees-110 degrees, 15.9% in 40 degrees-70 degrees, that is to say natural teeth are inclined to be of less undercut. CONCLUSION: The geometric relationship between crown contour and gingiva contour is so characteristic that contralateral tooth should be the only guidance to replace lost tooth.

Adult↗

PubMed2000

[Study on regulatory effect of composite taixian tablet on immune function of red blood cell in patients with oral lichen planus].

OBJECTIVE: To explore the regulatory effect of Composite Taixian tablet (CTXT) on red blood cell (RBC) immune function in patients with oral lichen planus (OLP) for the sake of providing the basis of clinical medication. METHODS: Sixty patients with OLP were assigned randomly into three groups and were treated by CTXT, Tripterygium hypoglaucum tablet and polyactin-A tablet respectively. And the changes of RBC-C3b receptor and immune circulating rosette complex on the surface of erythrocytes (RBC-ICR) were measured by saccharomycetic assay. RESULTS: Effect of CTXT was superior to that of Tripterygium hypoglaucum and polyactin-A tablets. CONCLUSION: CTXT is a relatively effective remedy with less side effect, it is worthy to be studied further.

Adult↗

PubMed2000

[Clinical study on endometrial ovarian cyst treated by integrated laparoscopy and Chinese herbal medicine].

OBJECTIVE: To investigate a new method of integrated laparoscopy with Chinese herbal medicine (CHM) in treating endometrial ovarian cyst. METHODS: One hundred and fifty-two patients with endometrial ovarian cyst were randomly treated with three therapies: Integrated laparoscopy with CHM (combination group), CHM (TCM group) and Danazol (WM group). To compare the clinical efficacy and side-effects of the three groups and to elucidate the therapeutic mechanism by detecting the serum CA125 reproductive endocrine hormone, plasma PGF1 alpha, PGE2 etc. RESULTS: The shrinking rate and disappearance rate of the cysts, and markedly effective rate of combination group were obviously higher than those of TCM group and WM group (P < 0.05, P < 0.01). The pregnancy rate of combination group was also higher than that of WM group (P < 0.05). Few side-effects were the most prominent advantage for combination group and TCM group. After treatment, the plasma PGF1 alpha lowered remarkably, serum p.o. increased and CA125 reduced obviously (P < 0.01, P < 0.05). CONCLUSION: The integrated laparoscopy and CHM combination therapy is a new method in treating endometrial ovarian cyst with significant efficacy, minimal adverse effect and maximal preservation of reproductive function.

Adult↗

PubMed2000

[Exercise induces increased CLUT4 gene expression and protein content in diabetic rats].

OBJECTIVE: To investigate the effects of exercise on insulin receptor, glucose transporter 4 (GLUT4) gene expression and protein content in skeletal muscle cells of streptozotocin-induced diabetic rats. METHODS: All rats were randomized into three groups: normal, diabetic and diabetic exercise groups. Diabetic exercise rats were swim-trained for 6 weeks. The GLUT4 mRNA of skeletal muscle was determined with dot blot and GLUT4 protein was detected by Western blotting. RESULTS: After 6 weeks of exercise training, plasma glucose level of diabetic rats decreased from 18.5 mmol/L +/- 1.9 mmol/L to 14.0 mmol/L +/- 3.3 mmol/L (P < 0.01). The dot blot revealed that GLUT4 mRNA decreased by 54.9% in skeletal muscle cells of diabetic rats compared with the normal rats. The Western blotting showed that GLUT4 protein of skeletal muscle cell in diabetic rats decreased by 30.7% compared with the control rats. GLUT4 mRNA and the protein of skeletal muscle in diabetic exercise group increased by 56% and 60.8% respectively as compared with the diabetic rats. However, the maximum binding of insulin receptor of skeletal muscle cells in diabetic exercise group decreased significantly compared with diabetic group. CONCLUSION: The weaker expression of GLUT4 gene and the lower level of GLUT4 protein may be one of the causes of hyperglycemia in diabetic rats. Exercise training may raise the GLUT4 gene expression and the amount of GLUT4 protein in diabetic rats, contributing to the main mechanism of improved post-receptor insulin resistance and decreased hyperglycemia in diabetic rats.

Animals↗

PubMed2000

[Fresh femoral shaft fractures treated with un-reamed intramedullary nail: 203 cases].

OBJECTIVE: To discuss the experience treating fresh femoral shaft fractures with un-reamed intramedullary nail. METHODS: 203 cases of fresh femoral shaft fractures who had been treated with un-reamed intramedullary nail from November. 1995 to January. 1999 were analyzed retrospectively. 18 cases were open, classified as Gustilo I, and. 185 cases were closed. Traction table and image intensifier were used during operation. Closed reduction, un-reamed and free hand distal locking technique were used. Active movements of nearby joints were encouraged and partial weight bearing of 10 - 15 kg were allowed right after operation. RESULTS: All 203 cases of fresh femoral shaft fractures got bone union within 4 - 6 months. No case of delayed union and infection happened. 1 distal locking screw was broken. All patients gained normal extremity functions. CONCLUSION: Un-reamed intramedullary nailing of fresh femoral shaft fracture has less damage to the local soft tissue and blood supply. It has a relative stable fracture fixation, and less interruption to the healing procedure. It is one of the best methods for treating femoral fracture.

Bone Nails↗

PubMed2000

[Multivariate analysis of prognostic factors in renal cell carcinoma].

OBJECTIVE: To assess the effect of prognostic factors on renal cell carcinoma (RCC). METHODS: 316 cases of RCC were reviewed retrospectively. Their survival rates were calculated by Kaplan-Meier method and statistical differences were determined by Log-rank test. Significant prognostic factors were evaluated by Cox's multivariate proportional hazard model. RESULTS: After 40.3 +/- 18.5 month follow-up, the overall 5-year survival rate was 62.3%. By multivariate analysis, nine factors were included in Cox's multivariate proportional hazard model. M was the most important prognostic factor in RCC (P = 0.0013), and the others in turn were T (P = 0.0182), age (P = 0.0347), performance status (P = 0.0423), N (P = 0.0471), lymphadenectomy (P = 0.0542), grade (P = 0.0775), serum albumin (P = 0.1536), and serum creatinine (P = 0.4543). CONCLUSIONS: The significant prognostic factors in RCC were T, N, and M. Age and performance status showed the effect on prognosis of RCC. Lymph-node dissection also revealed a meaningful effect on relative lower stage of RCC.

Adult↗

PubMed2000

[Surgical approaches of pheochromocytoma].

OBJECTIVE: To evaluate the effects of surgery of Pheo. METHODS: Pheochromocytoma was removed in 89 patients. RESULTS: In 39 patients, pheochromocytoma was removed by intercostal incision at the 11th rib and 15 at the 10th rib, 14 by transrectal incision, 9 by abdominothoracic incision, 5 by abdominal subcostal oblique incision, 3 by inferior abdominal incision, 3 by posterolateral incision. One patient received laparoscopic surgery. CONCLUSIONS: Pheochromocytoma definitely localized was removed by intercostal incision at the 10th or 11th rib. The suitable pathway for removal giant pheochromocytoma or pheochromocytoma related big vessels is transrectal incision or abdominothoracic incision. The incision of multiple pheochromocytoma or extra-adrenal pheochromocytoma may be in the middle of the abdomen.

Abdomen↗

PubMed2000

[Diagnosis and treatment of pheochromocytoma].

OBJECTIVE: To improve the diagnosis and treatment of pheochromocytoma. METHODS: From 1958 to 1998, 286 cases of pheochromocytoma, confirmed by open surgery and pathology, were retrospectively analyzed. RESULTS: The tumors in the adrenal gland were seen in 220 cases, and in the external adrenal gland in 66. Of 29 cases of malignant pheochromocytomas, 7 were noted in the adrenal and 22 in the external adrenal gland. 19 cases showed stationary pheochromocytomas without clinical features. Total resections were performed in 281 cases. CONCLUSION: The operative mortality could be reduced by the prevention of potential myocardiopathy. The diagnosis of malignant pheochromocytoma should depend upon the long-term follow up.

Adolescent↗

PubMed2000

[An experimental study on treatment of limbal alkali burn by allograft transplantation with cultured stem cells on amniotic membrane].

OBJECTIVE: To study the treatment of rabbit limbal alkali burn by cultured limbal stem cells growing on amniotic membrane for allograft transplantation. METHODS: After the primary culture of the rabbit corneal stem cells, they were cultured on amniotic membrane in DMEM/HamF12 medium for one week. Corneal stem cells and amniotic membrane were transplanted on the limbal and scleral area of the rabbit model with alkali burn. The corneal changes were observed by a slitlamp everyday, and the corneal pathological changes were examined. RESULTS: Cultured rabbit corneal stem cells continued to proliferate, differentiate and form multiple cell layers on amniotic membrane. After transplantation with cultured stem cells and amniotic membrane, the rabbit epithelium showed corneal phenotype and progressive decrease of vascularity and stromal infiltration in the limbal and peripheral zone. Pathological examination verified that the limbal and peripheral corneal epithelium was composed of multilayer cells, the neo-vascularization was reduced and stromal inflammatory cells were decreased. CONCLUSIONS: Allograft transplantation with cultured limbal stem cells can restore corneal epithelial cell composition, decrease neovascularization, maintain limbal cellular barrier function and provide better condition for keratoplasty later.

Alkalies↗

PubMed2000

[Study on the modulation of the inflammatory response in mouse hepatic vasculitis with sodium selenite and vitamin E antioxidants].

OBJECTIVE: To identify the regulatory effect of sodium selenite and vitamin E on complement-neutrophil-reactive oxygen species (ROS)-activation feedback mechanism-mediated inflammatory response. METHODS: The modulation of inflammatory response through the complement-neutrophil-ROS-activation feedback cycle with sodium selenite and alpha-tocopherol was verified both in vitro tests by chemiluminescense technique and complement fixation to detect ROS production and complement activation as well as in vivo mouse hepatic vasculitis models to test for the regulation of the inflammatory response. RESULTS: Convincing results were observed as the incidence of hepatic vasculitis dropped from 100% in the control group down to 20% in the seleno-antioxidant vitamin E treated group, indicating down regulation of the inflammatory response. CONCLUSIONS: Elucidation of the mechanism of complement mediated inflammatory response is a promising area for new therapeutic developments in the modulation of inflammatory response. This study suggests that selenium, vitamin E and other antioxidants may be the useful therapeutic agents in those disorders in which upregulation of inflammatory response has been implicated, e.g. ischemia, reperfusion injury, severe sepsis, rheumatoid arthritis and hepatitis.

Animals↗