Biomedical subjects
Publications and source records attributed to Y Uesugi.
alpha-L-Fucosidase isolated from the growth culture of Streptococcus sanguis ATCC 10557 acted on H- and Leb-blood group substances in porcine gastric lining, human gastric lining, human ovarian cyst fluid and human whole saliva, with consequent loss of H- and Leb -activities and a concomitant increase of Lea activity.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Movement and Metabolism of 32P and 35S-double labeled Kitazin P (S-benzyl O,O-diisopropyl phosphorothiolate) and 35S-labeled edifenphos (O-ethyl S,S-diphenyl phosphorodithiolate) were examined with three types of soils, sandy loam, alluvial clay loam, and volcanic ash loam. Vertical movement of both the compounds in soil column was different with soil types, and the order of mobility in soil column was as follows: sandy loam greater than alluvial clay loam greater than volcanic ash loam. Persistence of edifenphos in soil was shorter than that of Kitazin P. Main degradation products at the initial stage of metabolism were S,S,S-triphenyl phosphorotrithiolate, O,O-diethyl S-phenyl phosphorothiolate, S-phenyl dihydrogen phosphorothiolate and diphenyl disulfide in edifenphos and O,O-diisopropyl hydorgen phosphorothioate in Kitazin P. Sulfur atom of Kitazin P was found in sulfuric acid at a minor level through dibenzyl disulfide and toluene-alpha-sulfonic acid, and that of edifenphos was converted to sulfuric acid through diphenyl disulfide and benzenesulfonic acid. Kitazin P under flooded condition of alluvial clay loam was slightly more persistent as compared with upland condition. Sterilized condition of Kitazin P did not cause any appreciable degradation throughout the experimental period, but such condition did not necessarily prevent the degradation of edifenphos.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
We assessed the three-dimensional (3D) CT angiographic reconstruction of the splenic-portal venous system obtained by using multiple threshold display (MTD) in 40 patients with liver disease. In each patient, both shaded surface display(SSD) and MTD images were generated by using an Xtension with a Sun SPARCstation 20, and they were compared by two radiologists. MTD image quality was superior to that of SSD images, because MTD diminished longitudinal direction artifacts and the image depicted the small vessels clearly. Based on the above results, we consider MTD to be a useful method for 3D-CT angiography of the splenic-portal venous system.
To characterize and compare maxillary incisor lesions caused by various antitumor drugs, male BALB/c mice were given a single intravenous injection of an estimated 10% lethal dose (LD10)) of 5-fluorouracil (5-FU), adriamycin (ADR), mitomycin C (MMC), vinblastine sulfate (VBL). taxotere (TXR), irinotecan hydrochloride (CPT-11), DX-8951f, or cisplatin (CDDP). After 3, 5, 10, 15, and 60 days, the animals were sacrificed, and the maxillary incisors were examined microscopically. The dental lesions observed were classified into 4 different types on the basis of their morphological features. The lesion due to 5-FU was characterized by focal defects in the dentin, and this injury was reversible (transient dentin injury). ADR- or MMC-induced lesions were defined by abnormal structure of the apical aspect of the tooth and irregular odontogenesis, lasting for a long period (persistent apical injury). Treatment with VBL or TXR showed irregular enamel formation and abnormal dentinogenesis. Their targets were considered to be both immature and mature odontogenic cells (diffuse dental injury). Exposure to CPT-11, DX-8951f, or CDDP elicited minor reductions in a few precursor cells in the epithelial sheath on day 3, but no prominent dental abnormalities were seen thereafter (nontoxic injury). In conclusion, antitumor drugs can cause a variety of dental lesions that vary temporally and spatially, making histopathological examination of the maxillary incisor an important component of the safety assessment process for novel antitumor drugs.
To evaluate the clinical usefulness and limitations of three-dimensional (3-D) imaging of laryngeal cancers by high-speed helical (spiral) CT scanning, 3-D images were reconstructed for one dissected human larynx and 10 patients with laryngeal cancer. The larynges were scanned in 1- to 2-mm slices, and were reconstructed using a slice thickness of 0.5-1.0 mm. The macroscopic (or endoscopic) findings and the 3-D CT images of the larynx were compared. The selected threshold CT values were -600 HU (Hounsfield units) to -100 HU for the mucous membranes, and 250 HU for bone. Under these conditions, almost all of the structures remained distinct. The 3-D images of the larynx obtained by helical CT were very helpful in understanding laryngeal anatomy, especially in the subglottic area, which cannot be seen clearly by endoscopy or conventional axial CT. Clinically, this system would have advantages in the detection of subglottic cancers, or the invasion of glottic or supraglottic cancers into the subglottic area.
To evaluate the usefulness of stereoscopic images of larynges using helical CT in stereo mode, a retrospective review of the characteristics of stereoscopic viewing of larynges was made. The subjects were 3 patients with laryngeal cancer, 1 patient with laryngeal leiomyosarcoma and 1 patient with an advanced tongue carcinoma whose formalin-fixed larynx was extirpated. The larynges were scanned by high-speed helical CT using 1- to 2-mm slices. The reproduction of stereographic images was performed by the manipulation and rotation of three-dimensional structures around the y-axis on the computer display. The three-dimensional images of the complex structures, such as the arytenoid cartilage, aryepiglottic fold and pyriform sinus, were better observed by binocular images (stereograms) than by monocular images. Stereoscopic views of the larynx are useful in producing three-dimensional images of the unseen inner surface of the human body.
alpha-L-Fucosidase acting on naturally occurring substrates was highly purified from the growth culture of Streptococcus sanguis ATCC 10557. The molecular weight of the enzyme was approximately 120,000 and the optimal pH was at 5.5. The purified enzyme showed specificity toward the linkage of alpha-(1 leads to 2) fucosides in oligosaccharides and glycoproteins. The enzyme released L-fucose from glycoprotein in human parotid saliva.
OBJECTIVE: To assess the image quality of multiple threshold display (MTD) as a new technique for generating three-dimensional (3D) pulmonary computed tomographic (CT) angiographic images. METHODS: We used MTD, a type of shaded surface display (SSD) offering the selection of multiple thresholds and transparencies, to reconstruct 3D-CT angiograms from enhanced helical CT data sets in 33 patients with lung disease. In MTD, eight thresholds of CT values are selected, and transparency is assigned to each. The selected voxels, ranging from -600 to 1,000 Hounsfield Units, were divided into eight classes, and transparency ranging from 0 to 100% was assigned to each. The CT scanner employed was a Toshiba Xvigor. MTD and SSD images were generated by using an Xtension with a Sun SPARC station 20, and they were compared by two radiologists. RESULTS: The image quality of MTD images was superior to that of SSD images (p < 0.01), because the MTD images demonstrated clearly both the major and small vessels. CONCLUSION: MTD is a useful technique for 3D pulmonary CT angiography.
In 35-day-old female ICR/JCL mice given 5 daily injections of 1 microgram diethylstilbestrol (DES) from the day of birth, a significantly higher incidence of polyovular follicles was found in the ovaries than in those of age-matched control mice. Gap junctions of granulosa cells of mature follicles in neonatally DES-exposed mice were larger than those of the controls. When stimulated by gonadotropins (PMSG and hCG) and caged with males, the number of tubal embryos in DES-exposed mice was less, but the rate of fertilization and development was not different compared to the controls. Division of oocytes collected from the ovaries of 40-day-old DES-exposed and control mice after stimulation of gonadotropins was examined 24 to 72 h after in vitro insemination to ascertain whether fertilization had occurred in oocytes from polyovular follicles. Seventy-seven % of oocytes from uniovular follicles of control mice developed up to 8-cell stage embryos following in vitro insemination; 66% of those from similar follicles of DES-exposed mice developed into the same stage. By contrast, only 47% of oocytes from polyovular follicles of DES-exposed mice showed the division up to 8-cell stage 72 h after insemination, indicating a significantly lower fertilization rate compared to the oocytes from uniovular follicles of control and DES-exposed mice. Without insemination, oocytes taken from the same pools in these experiments never divided during the period of manipulation and incubation.(ABSTRACT TRUNCATED AT 250 WORDS)
The purpose of this study was to evaluate the clinical usefulness of three-dimensional (3D) images of the bronchi obtained using helical CT. Thirteen patients with lung cancer, one with tracheal diverticulum, and one with bronchial amyloidosis were examined. The CT scanner employed was the Toshiba Xforce. The helical CT scan cycle consisted of 20 continuous rotations, each requiring 1.5 sec, for a total scanning time of 30 sec. Scans were obtained using a 5-mm X-ray beam width, a 5-mm/1.5 sec couchtop sliding speed, and a 2-mm reconstruction interval. 3D images were reconstructed using a CEMAX VIPstation. The optimal lower and upper threshold CT values for 3D images of the bronchi were -650 and -100 HU, respectively, and 3D images clearly depicted endobronchial lesions. Cartilage crescents were also demonstrated, but longitudinal and circular mucosal folds could not be visualized. In conclusion, 3D images of the bronchi acquired using helical CT were useful in evaluating endobronchial lesions.
To evaluate the clinical usefulness of helical scanning CT for hepatocellular carcinoma, 39 patients were examined using a Toshiba CT system, the Xforce. Helical scanning CT data were acquired using up to 20 continuous 1.5-sec rotations, with contrast medium bolus injection of 100-150 ml at a rate of 1.5-3 ml/sec, and during a single breath-hold. Scanning was begun 35 to 50 sec after the start of injection. Helical scanning CT permits axial images to be reconstructed at any desired position within the scanned area, and provides images without interslice gaps caused by respiratory movement. Contrast enhanced helical scanning CT permits blood vessels to be clearly displayed following the bolus injection of contrast medium using an automatic injector. Furthermore, the shorter scanning time makes it possible to scan the target area in the desired vascular phase. In all 39 patients who were examined using contrast enhanced helical scanning CT, high-quality three-dimensional images were obtained. Such images were particularly useful for assessing the three-dimensional relationships among high density tumors and vascular structures. In conclusion, helical scanning CT was considered to be useful for hepatocellular carcinoma.
To evaluate the clinical usefulness of three-dimensional (3D) images of pulmonary nodules acquired using helical CT, 3D reconstructions were performed in 32 patients using a CEMAX VIPstation. The CT scanner employed was the Toshiba Xforce. Helical CT data were acquired using up to 20 continuous 1.5-sec rotations with an X-ray beam width of 5 mm and a couchtop movement speed of 5 mm/1.5 sec. Axial images were reconstructed at a section interval of 2 mm. The optimal lower and upper threshold CT values for 3D images were as follows: 1) solid pulmonary nodule (-700--400/-100 HU), 2) tumor invading pleura or chest wall (-700--400/-200 HU), 3) pulmonary nodule with cavity (-700--400/50 HU), 4) small pulmonary nodule (< 10 mm) (-750--650/-100 HU), and 5) arteriovenous malformation (180/500 HU). In all cases, it was possible not only to demonstrate abnormal findings three-dimensionally but also to grasp anatomical relationships among the pulmonary nodule, bronchi, vessels, and chest wall.