Biomedical subjects

Y Ueda

Publications and source records attributed to Y Ueda.

1,329 records · Page 74Linked to original sources

PubMedDate not supplied

Opsoclonus-myoclonus syndrome with abnormal magnetic resonance imaging and brainstem auditory evoked potentials.

We describe two patients in encephalitis with opsoclonus-myoclonus syndrome. Cranial magnetic resonance imaging demonstrated an area of hyperintensity on the T2-weighted images in the left tegmentum at the pons level (case 1) and in the left tegmentum and partly basis at the upper pons level (case 2), respectively. On the other hand, brainstem auditory evoked potentials following left monaural stimulation and ipsilateral records revealed a delay of IIIrd wave latency and prolongation of I-III interpeak latencies (case 1). From these findings we suggest that the locus of the lesion of opsoclonus-myoclonus syndrome may partly exist in the brainstem (pontine tegmentum).

Adult↗

PubMedDate not supplied

A case of polymyositis associated with chronic thyroiditis presenting as hyperthyroidism.

We describe a case of polymyositis associated with hyperthyroidism (Graves' disease). Clinical findings and thyroid function tests revealed hyperthyroidism. On histological examination, it was found that the thyroid gland was involved in chronic thyroiditis, and no appearance of Graves' disease was noted. This case suggested that the patient's muscle weakness might have been related to a disturbance of thyroid function, that is, coexistence of subclinical thyrotoxic myopathy. There were fluctuations in the levels of various antibodies during the clinical course of this overlap syndrome.

Female↗

PubMedDate not supplied

Movement disorder with abnormal copper metabolism--a case report and review of the literature.

We describe a neurological disease, seen in the elder sister of identical twins, with dysarthria, involuntary movements, spastic gait, slightly low serum copper, borderline low to normal serum ceruloplasmin, normal urinary copper, and a high hair copper concentration. This neurological disorder appears to differ from others associated with abnormal copper metabolism such as Wilson's or Menkes' kinky hair disease.

Adult↗

PubMedDate not supplied

A case of neurogenic orthostatic hypertension.

A patient with orthostatic hypertension of neurogenic origin is reported. The Schellong and head-up tilt tests produced a sudden moderate to marked increase of the blood pressure, but the plasma renin activity and plasma aldosterone level showed a normal response. The cold pressor test also produced a moderate to marked increase in the blood pressure. Pharmacological cardiovascular autonomic nervous function tests showed a decrease of parasympathetic tone and denervation hypersensitivity of the sympathetic nerve terminals. These findings suggest that the orthostatic hypertension in this patient was mainly due to sympathetic denervation hypersensitivity.

Adrenal Glands↗

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Successful treatment of acute right cardiac failure due to pulmonary thromboembolism in mixed connective tissue disease.

Mixed connective tissue disease (MCTD) is characterized as a benign rheumatic disease with a favorable response to therapy. When pulmonary hypertension is a complication, however, it is often reported to be fatal. A 32-year-old female patient with MCTD who had developed rapidly progressive pulmonary hypertension and disseminated intravascular coagulopathy was admitted to our hospital and was successfully treated with corticosteroids and anticoagulants. The failure of microcirculation due to coagulopathy is considered to be one of the possible entities of pulmonary hypertension.

Adult↗

PubMedDate not supplied

Microtubular aggregates in the rough endoplasmic reticulum of sacrococcygeal chordoma.

A tumor with the light microscopic and immunohistologic characteristics of chordoma in the sacrococcygeal region in a 48-year-old man was examined by electron microscopy and immunohistochemistry for tubulin. A large population of cells exhibited prominent parallel arrays of long, straight microtubules in the rough endoplasmic reticulum in addition to the well-described ultrastructural features of chordoma. Immunoreaction to tubulin recognized in the juxtanuclear regions of the large number of tumor cells was in accordance with the ultrastructural localization of the microtubular aggregates. This seems to be the first report of microtubular aggregations in rough endoplasmic reticulum in classic chordoma. Furthermore, the present electron microscopic and immunohistochemical findings suggest that tubulin is a constituent of the unusual structures.

Aged↗

PubMedDate not supplied

A case of aberrant pancreatic cancer in the jejunum.

We report a case of aberrant pancreatic cancer of the jejunum in a 63 year-old man. The patient was admitted to our hospital with epigastric discomfort and vomiting due to obstruction of the jejunum. Laparotomy revealed a submucosal tumor on the jejunum with multiple liver metastases. Histological examination showed the tumor to be a well differentiated tubular adenocarcinoma originating from aberrant pancreatic tissues lacking islets. Only 1 case of aberrant pancreatic cancer in the jejunum has been previously reported in the literature.

Adenocarcinoma↗

PubMedDate not supplied

p27 expression correlates with prognosis in patients with hypopharyngeal cancer.

BACKGROUND: Hypopharyngeal cancer is closely associated with smoking, drinking, and malnutrition. Recent findings reveal that carcinogenesis depends on genetic aberrations. MATERIALS AND METHODS: Eighty-one patients with hypopharyngeal cancer were analyzed for p53, CCND1, and p27, and 72 patients were analyzed for p21, immunohistochemically. RESULTS: p53 overexpression was found in 70%, CCND1 in 17%, p21 in 29%, and p27 in 32%. The low intensity of p27 expression correlated with poor prognosis, but the level of expression of p53, CCND1, and p21 did not. CONCLUSION: The immunohistochemical analysis of p27 provides a possible tool in determining the prognosis of patients with hypopharyngeal cancer.

Cell Cycle Proteins↗

PubMedDate not supplied

Sensitization and caffeine potentiation of cisplatin cytotoxicity resulting from introduction of wild-type p53 gene in human osteosarcoma.

The present study was performed to investigate whether the introduction of a wild-type p53 gene into human osteosarcoma cells could alter the growth rate and enhance the cytocidal effect of cisplatin (CDDP) and the synergistic antitumor effect of caffeine. The lipofection method was used to transfect a wild-type p53 expression plasmid into the human osteosarcoma cell line, Saos2, which has both p53 alleles deleted. The transfected cells, Saos2/p53, had a reduced growth rate compared with the parental cell line. The colorimetric WST-1 assay demonstrated that Saos2/p53 cells were twice as sensitive to CDDP alone at a 50% inhibition concentration than the parental Saos2 cells. Caffeine significantly potentiated the cytocidal effect of CDDP in the Saos2/p53 cells. Furthermore, the TUNEL assay revealed that following treatment both with CDDP alone and with CDDP combined with caffeine, a higher percentage of the Saos2/p53 cells underwent apoptosis than did the parental Saos2 cells. Therefore the cytocidal effect of CDDP and the synergistic antitumor effect of caffeine are enhanced by the introduction of a wild-type p53 gene into a human osteosarcoma cell line null for p53. This raises the possibility that gene therapy using the p53 gene may prove efficatious for human osteosarcomas lacking p53 and which are resistant to standard chemotherapy.

Apoptosis↗

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Chronic aortic dissection (DeBakey type II) with infective thrombus in the false channel.

A 68-year-old man with a DeBakey type II chronic aortic dissection developed bacteremia secondary to a urinary tract infection with the Serratia species. This resulted in an infected thrombus in the false channel and a subsequent rupture of the ascending aorta. An emergency resection of the ascending aorta and a Dacron graft interposition were performed. The patient suffered from severe neurological insufficiency and died of pneumonia 6 months postoperatively. The optimal operation time and surgical options of an infected aortic dissection should be determined.

Aged↗

PubMedDate not supplied

Calcitonin gene-related peptide containing sensory neurons innervating tooth pulp and buccal mucosa of the rat: an immunohistochemical analysis.

Differential peptidergic innervation of the rat buccal mucosa and incisor tooth pulp was demonstrated by experimental immunohistochemistry. In the buccal mucosa, calcitonin gene-related peptide (CGRP)-like immunoreactive (IR) nerve fibres were observed in both the epithelium and submucosal layer. Most of these fibres were also immunoreactive to substance P (SP). On the other hand, SP-IR fibres were less numerous than CGRP-IR fibres in the incisor tooth pulp. Fast blue (FB) was applied to the buccal mucosa and the incisor pulp chamber. Small (less than 20 microns) FB-labelled CGRP-IR neurons were more numerous in the buccal mucosa group, while medium to large neurons (20-50 microns) predominated in the incisor pulp. Following neonatal capsaicin treatment, CGRP- and SP-containing fibres in the buccal mucosa almost totally disappeared, but many CGRP-IR fibres remained in the incisor tooth pulp. The findings suggest that these two oral tissues are innervated by different subgroups of CGRP-IR neurons in the trigeminal ganglia.

Animals↗

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Surgical treatment of aneurysm or dissection involving the ascending aorta and aortic arch, utilizing circulatory arrest and retrograde cerebral perfusion.

Recently we replaced the ascending aorta and aortic arch in 8 patients with aneurysm or dissection, using profound hypothermic circulatory arrest with retrograde cerebral perfusion. There were no operative deaths. Open aortic anastomosis facilitated repair of the aortic arch without clamping the arch tributaries, and embolism due to particulate debris from clamping of the arch vessels was eliminated. Retrograde cerebral perfusion during profound hypothermic circulatory arrest is a simplified technique that may protect the brain. This method offers advantages over previously described methods, particularly in obviating dissection of the arch tributaries and the clamping thereof, and in protecting the central nervous system.

Aged↗

PubMedDate not supplied

Repair of intracardiac defects associated with congenitally corrected transposition of the great arteries.

From October 1976 to June 1986, 12 patients with corrected transposition of the great arteries (c-TGA) underwent repair of associated intracardiac defects. Ventricular septal defect (VSD) was closed by the method of de Leval in 7 patients, pulmonary outflow tract obstruction was relieved by valvotomy in 2, and bypassed by an external valved conduit in 6. The systemic atrioventricular (A-V) valve was replaced in 2. There was one operative death, giving a mortality rate of 8%. None of the patients developed complete heart block. The ejection fraction of the systemic ventricle was impaired (29-38%), particularly after ventriculotomy of this chamber. Surgical repair of intracardiac defects associated with c-TGA currently can be performed with acceptable risk, and de Leval's method for VSD closure is recommended; but the possibility of the postoperative development of systemic ventricular dysfunction and A-V valve regurgitation necessitates careful follow-up.

Adolescent↗

PubMedDate not supplied

Current aspects of the pathology of osteosarcoma.

Since the introduction of standardized chemotherapy protocols of osteosarcoma a lot of new aspects in prognosis and curability of these have best developed. Current subclassification which divided osteosarcoma into a conventional type and eleven important recognizable varieties is one of the reason for this success. Cytological grading also serves as a good indicator for the prognosis and is an important criterion for application of adjuvant chemotherapy. Several structure proteins of the extracellular matrix have gained importance in making the diagnosis of an osteosarcoma. Immunohistochemically and biochemically evaluations could show that different collagenous-proteins can be useful for the differential diagnosis of bone tumors. The integration of molecular pathologic methods into the structural morphologic findings will be helpfull in the identification of mutated structure proteins. Oncogenes and tumor suppressor genes are of major importance for the tumorigenesis of osteosarcoma. The prognostic significance of the inactivation of p53 and RBI gene remains to be elucidated. Resistance to chemotherapy is the major mechanism responsible for the failure of osteosarcoma treatment. The main cause for this failure is multidrug resistance, which is often related to a plasma membrane protein, the P-glycoprotein. Immunohistologic investigations of P-glycoprotein are not sufficient to demonstrate the possible association between overexpression of this protein and tumor progression.

Bone Neoplasms↗

PubMedDate not supplied

Establishment and characterization of an acquired cisplatin-resistant subline in a human osteosarcoma cell line.

A cisplatin (CDDP)-resistant human osteosarcoma cell line (OST/R) was established by continuous exposure to CDDP. OST/R cells proved to be 6.73 times more resistant to CDDP compared with parental OST cells, and showed cross-resistance to carboplatin (CBDCA). The mechanism of CDDP resistance was a significant decrease of intracellular platinum accumulation which was 40% of that in OST cells. OST/R cells were exposed to CDDP for 6 hours, the platinum was released from the cytoplasm of OST/R cells without reaching a state of equilibrium. DNA synthesis in OST/R cells was not inhibited by CDDP exposure, while in OST cells it was reduced by 50%. These data provide the first evidence that the increased efflux of platinum may play an important role in CDDP-resistance.

Antineoplastic Agents↗