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Biomedical subjects

Y Toyoda

Publications and source records attributed to Y Toyoda.

At least 307 records · Page 17Linked to original sources
PubMed1979

The localization of mutarotase in rat kidney.

The localization of mutarotase in rat kidney was investigated by fluorescein-labelled and peroxidase-labelled antibody techniques, and by method of isolation of the nuclei and cytoplasm in non-aqueous solvents. In these immunohistochemical studies, mutarotase was almost exclusively recognized in the nuclei of epithelial cells of renal tubules and glomeruli in rat. The specific activity of mutarotase was found to be 1.5 times higher in the nuclei (122 units/g dry wt) than that in the cytoplasm (80 units/g dry wt) isolated with non-aqueous solvents. These results suggest that mutarotase may be involved in the metabolism of D-glucose in nuclei.

Animals↗
PubMed1977

A new technique for catheterless cutaneous ureterostomy.

A new technique for cutaneous ureterostomy is described. Essential conditions for the successful avoidance of stomal stenosis are the firm fixation of the anastomosis to the skin and healing by first intention. Nipple formation is not an absolute must. In order to fulfill these requirements I devised a new technique in which the distal end of the ureter is cut longitudinally to make a fish-mouth-like opening and each separated ureteral segment is sutured to the corresponding skin area. This area is deprived of its epidermis and dermis. Results of the technique have been satisfactory to date.

Aged↗
PubMed1977

[Artificial insemination in the mouse with special reference to the effect of sperm numbers on the conception rate and litter size (author's transl)].

Mature female mice (ICR-JCL), 8 to 12 weeks of age, were artificially inseminated at 8:30-9:30 a. m. on the day of estrus vaginal smear (about 3-7 hr after ovulation) with 3.18 X 10(6), 1.83 X 10(6) and 1.15 X 10(6) sperms from four, two and one cauda epididymidis, respectively, of adult males which were suspended in 50 microliter of a modified Krebs-Ringer-bicarbonate solution and incubated at 37 degrees C was under 5% CO2 in air for an hour. Immediately after insemination, pseudopregnancy induced by an artificial penis and a vaginal tampon. Out of 13 females inseminated with 3.18 X 10(6) sperms, 9 females showed placental signs and 8 of them gave birth to the mean 10.5+/-2.20 (M+/-S.D.) young at term. Four of 13 females having received 1.83 X 10(6) sperms became pregnant giving birth to the mean 4.3+/-2.1 (M+/-S.D.) young at term. On the other hand, 5 out of 6 females failed to become pregnant following insemination with 1.15 X 10(6) sperms, and only one showed a placental sign and gave birth to twelve young at term. It is concluded that the conception rate and litter size are both dependent on the number of sperms inseminated and that more than 3 X 10(6) sperms are necessary to get the conception rate and the litter size comparable to those in natural mating.

Animals↗