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Biomedical subjects

Y Satoh

Publications and source records attributed to Y Satoh.

At least 559 records · Page 31Linked to original sources
PubMed1985

[Sinus histiocytosis in the lymph nodes of breast cancer patients having undergone extensive radical mastectomy].

Sinus histiocytosis in the regional lymph nodes of 45 patients with breast cancer who had undergone extended radical mastectomy was studied by histological examination of 2,294 resected lymph nodes. Sinus histiocytosis was divided into three grades, marked, moderate, and mild to negative, by Ohmori's classification. The results indicated that: 1) sinus histiocytosis correlates fairly well with clinical stage, especially with the n-factor, and 2) the degree of sinus histiocytosis shows little difference between three groups of regional lymph nodes. The authors concluded that sinus histiocytosis dominates the prognosis and represents some aspect of a reticuloendothelial response of the whole body to carcinoma.

Breast Neoplasms↗
PubMed1984

Ultrastructural localization of thiamine pyrophosphatase activity in the intestinal epithelial cells of adult rats.

Ultrastructural localization of thiamine pyrophosphatase (TPPase) activity was studied in the small and large intestinal epithelial cells of adult rats. In the duodenal and jejunal absorptive cells, TPPase activity was localized on the microvilli, on the lateral cell membrane, and on the lysosome-like bodies. In the ileal epithelial cells, TPPase activity was detected on the lysosome-like bodies, and weak phosphatase activity was observed on the microvilli and on the cisternae of the rough-surfaced endoplasmic reticulum (rER). In the principal cells of the caecum, the ascending colon, and the descending colon, the lysosome-like bodies and the cisternae of the rER showed TPPase activity. Almost all Golgi lamellae, vesicles and GERL elements (NOVIKOFF 1964) in the duodenal absorptive cells were strongly TPPase positive. The 1st to 3rd of the inner Golgi lamellae and the vesicles of the jejunal, ileal, and large intestinal epithelial cells usually showed positive TPPase activity, while the GERL elements were totally negative.

Animals↗
PubMed1984

Ultrastructural localization of acid phosphatase activity in the jejunal absorptive cells of fasted rats.

Ultrastructural localization of acid phosphatase activity was investigated in the jejunal absorptive cells of 21 d fasted rats (about 500 g). The enzyme activity was localized on the membrane of microvilli, the lateral cell membrane, the Golgi complex, the lysosomes and the GERL of Novikoff (a part of the smooth surfaced endoplasmic reticulum located in close proximity to the Golgi saccules) of jejunal absorptive cells. Moreover, the lysosomes of various sizes and shapes with acid phosphatase activity was characteristically encountered in the infranuclear cytoplasm. The lysosomes appeared to be autolysosomes .

Acid Phosphatase↗
PubMed1984

A method to potentiate enteral absorption of interferon and selective delivery into lymphatics.

The potentiated absorption of human fibroblast interferon (HuIFN-beta) with the aid of lipid-surfactant mixed micelles from the rat large intestine and lymphatic delivery were studied. The administration of HuIFN-beta with saline solution alone into the lumen of the large intestine indicated no detectable HuIFN-beta in the serum or the lymph for 5 h. Neither lipid (linoleic acid) nor surfactant ( HCO60 , polyoxyethylene hardened castor oil derivative) could promote the absorption of HuIFN-beta. However, the administration with linoleic acid- HCO60 mixed micelles enabled the great absorption of HuIFN-beta from the large intestine, and HuIFN-beta was delivered into the lymphatics with an extremely high selectivity.

Animals↗
PubMed1984

Cytoplasmic inhibiting factors for 3H-dexamethasone binding to glucocorticoid receptors in rat tissues.

Factors which specifically inhibit 3H-dexamethasone binding to unoccupied glucocorticoid receptors were examined in cytosols of the seminal vesicle and ventral prostate of rats. The inhibiting factors are mostly precipitated at 40-50% saturation of (NH4)2SO4 and eluted from a Sephacryl S-200 column immediately after the elution volume of bovine serum albumin. The inhibitory activity was relatively heat-stable and decreased to approximately 35% of the initial activity by trypsin digestion. These results suggest that the inhibiting factors are of a polypeptide-like nature. The inhibitory activity of the factors seems to be blocked by treatment with dithiothreitol and molybdate. The inhibiting factors act to decrease the binding affinity of glucocorticoid receptors to 3H-dexamethasone without altering the number of the available binding sites in cytosols. These results indicate that the binding of glucocorticoid receptors to ligands in vitro is influenced by a number of factors.

Adrenalectomy↗
PubMed1984

Glucocorticoid receptor in the rat uterus.

3H-Dexamethasone binding sites with a Kd of approximately 0.7 nM and a maximum number of binding sites of approximately 0.3 pmoles/mg protein were demonstrated in the uterine cytosol of adrenalectomized rats only if dithiothreitol was present in the incubation mixture and the simultaneous presence of molybdate further enhanced the binding in the cytosol. The binding sites exhibited a high specificity for glucocorticoids and were depleted in a dose-dependent manner from cytosol after administration of dexamethasone to animals. The depletion was not due to the occupation of the binding sites by the dexamethasone administered and the rate of depletion was correlated with the inhibition of uterine growth induced by estrogen administration. The cytosol labeled with 3H-dexamethasone in the presence of dithiothreitol bound to DNA-cellulose efficiently after heating at 25 degrees C for 30 min and the binding was inhibited by pyridoxal 5'-phosphate added to the reaction mixture. The effect of heating on the DNA-cellulose binding was abolished by molybdate in the incubation mixture. From these observations, it was concluded that 3H-dexamethasone binding sites in the rat uterus were physiologically active glucocorticoid receptors.

Adrenalectomy↗
PubMed1984

Stability of receptor complexes in the rat liver bound to glucocorticoids of different biopotencies.

To examine the behavior in the receptor-acceptor system of glucocorticoids of different biopotencies, the stability of receptor complexes of dexamethasone (Dex), prednisolone (Pred) and corticosterone (Cort) in cytosols, nuclei and nuclear extracts from the rat liver was compared. Receptor complexes bound to these ligands were relatively stable at 0 degrees C, but at 25 degrees C a rapid liberation of ligands was observed. However, differences in the rate of temperature-dependent decay of these receptor complexes were obvious; the Dex receptor complex was the most stable, the receptor complex bound to Cort liberated the ligand most rapidly and the stability of the Pred-receptor complex was the intermediate of these two. The addition of molybdate and dithiothreitol stabilized the receptor complexes in cytosols but these agents accelerated the liberation of ligands from the complexes in nuclei and nuclear extracts. Among the factors examined, only bovine serum albumin decreased the rate of decay in the nuclear-bound receptor complexes. From these observations, it appears likely that different mechanisms may contribute to the dissociation of ligand from receptor complexes in cytosols, nuclei and nuclear extracts.

Animals↗
PubMed1984

Ultrastructure of Paneth cells in germ-free rats, with special reference to the secretory granules and lysosomes.

Ultrastructure and acid phosphatase activity in Paneth cells, which are thought to play a role in regulation of the intestinal flora, were observed in control and germ-free rats (5 weeks of age, male). Examination of the germ-free rats revealed: secretory granules with coarse and a low-electron dense matrix in the duodenal, jejunal and ileal Paneth cells; numerous and larger lysosomes in ileal Paneth cells; and contact or fusion between the secretory granules and lysosomes in ileal Paneth cells. Acid phosphatase activities in Paneth cells were observed in the GERL of Novikoff (1964) and lysosomes in germ-free as well as control rats. None of the secretory granules showed any acid phosphatase activity. The fact that the lysosomes fused with the secretory granules suggests that the process of crinophagy occurred in the Paneth cells of germ-free rats.

Acid Phosphatase↗
PubMed1984

[Fundamental and clinical evaluation of ceftriaxone in the field of pediatrics].

Fundamental and clinical evaluation on ceftriaxone (Ro 13-9904, CTRX) was performed in the field of pediatrics and the following results were obtained. The antibacterial activity of CTRX was determined against clinically isolated strains at our department. CTRX was definitely superior to CEZ and CMZ and almost equal or slightly superior to CTX in activity against Gram-negative bacteria, while the MIC of CTRX against Gram-positive bacteria was higher than that of CEZ and CMZ and about equal to that of CTX. The blood concentration of CTRX after one shot intravenous injection with 10 mg/kg was 67.98 micrograms/ml at 15 minutes, 51.96 micrograms/ml at 30 minutes, 37.51 micrograms/ml at 1 hour, 28.91 micrograms/ml at 2 hours, 20.71 micrograms/ml at 4 hours, 13.97 micrograms/ml at 6 hours and 6.45 micrograms/ml at 12 hours, while the half-life time was 3.74 hours. The blood concentration of CTRX after one shot intravenous injection with 20 mg/kg was 179.55 micrograms/ml at 15 minutes, 120.01 micrograms/ml at 30 minutes, 100.01 micrograms/ml at 1 hour, 53.75 micrograms/ml at 2 hours, 33.13 micrograms/ml at 4 hours, 26.41 micrograms/ml at 6 hours and 21.49 micrograms/ml at 12 hours, while the half-life time was 4.15 hours. The blood concentration of CTRX after intravenous drip infusion for 1 hour with 10 mg/kg was 19.54 micrograms/ml at 15 minutes, 27.19 micrograms/ml at 30 minutes, 36.57 micrograms/ml at 1 hour, 23.83 micrograms/ml at 2 hours, 19.69 micrograms/ml at 3 hours, 14.46 micrograms/ml at 5 hours, 11.02 micrograms/ml at 7 hours and 7.27 micrograms/ml at 13 hours, while the half-life time was 6.59 hours. The blood concentration of CTRX after intravenous drip infusion for 1 hour with 20 mg/kg was 61.72 micrograms/ml at 30 minutes, 108.1 micrograms/ml at 1 hour, 54.95 micrograms/ml at 2 hours, 35.68 micrograms/ml at 3 hours, 28.13 micrograms/ml at 5 hours, 20.51 micrograms/ml at 7 hours and 11.43 micrograms/ml at 13 hours, while the half-life time was 4.23 hours. There was noticed a tendency of the blood level being elevated by consecutive administration. The urinary recovery rate of CTRX ranged from 36.5 to 71.6%. The excretion rate of CTRX into the cerebrospinal fluid ranged from 5.2 to 11.6%. The excretion rate of CTRX into the pleural fluid was 31.0%. The clinical efficacy rate was 87.5% (excellent or good) in 8 children with infections treated with CTRX. The eradication of bacteria was observed in all of 5 cases bacteriologically evaluation.(ABSTRACT TRUNCATED AT 400 WORDS)

Adolescent↗
PubMed1984

[Fundamental and clinical evaluation of ceftriaxone in the pediatric field].

Fundamental and clinical evaluation of ceftriaxone (CTRX) was performed in the pediatric field and the following results were obtained. The MIC of CTRX against E. coli isolated from urinary tract infections in children ranged from less than or equal to 0.024 to 0.39 mcg/ml except for 1 strain. CTRX was superior to other 3rd generation cephalosporins such as CPZ and LMOX, showing effectiveness also against ABPC-resistant bacteria. The clinical efficacy and bacteriological efficacy in 6 children consisting of 5 with respiratory tract infections and 1 with urinary tract infection were 83% and 100%, respectively. As to the adverse reaction, diarrhea was observed in 2 cases. The determination of PIVKA-II performed during the therapy with CTRX, which is observed when vitamin K is deficient, showed positiveness in 2 cases out of 6 cases including 1 which the clinical efficacy could not be evaluated. The test of platelet function in 3 cases found no inhibition of agglutination. Twice-daily administration with 20 mg/kg CTRX was considered to be a useful and safe method for treatment of bacterial infections in children, although attention should be taken not to cause vitamin K deficiency as in other 2nd and 3rd generation cephalosporins.

Cefotaxime↗
PubMed1984

[Renal angiomyolipoma: report of 2 cases of tuberous sclerosis in female siblings].

Two cases of renal angiomyolipomas in female siblings associated with tuberous sclerosis were reported. The first case was a 47-year-old housewife who visited the hospital because of severe right flank pain. Bilateral renal angiomyolipomas and hemorrhage in the right angiomyolipoma were recognized by computerized tomography, ultrasonography and renal angiography. After 2 months she had another episode of left flank pain and was diagnosed to have hemorrhage in the left renal tumor. The pain remissed by conservative treatment. Bilateral nephrectomy and hemodialysis would be necessary if she were to have a third attack. The second case was her 36-year-old sister with the chief complaint of left flank pain and genital bleeding. CT and renal angiography suggested a large angiomyolipoma in her left kidney and a small one in her right kidney. Left nephrectomy, right partial nephrectomy and hysterectomy were performed to prevent intratumoral hemorrhage. The histopathological diagnosis was angiomyolipoma of kidneys, uterus and lymphnodes of right renal hilus. Although pre-operative differentiation of angiomyolipoma from renal cell carcinoma has been difficult, recently diagnosis has become possible by CT, ultra-sonography and angiography.

Adult↗