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Biomedical subjects

Y Oshida

Publications and source records attributed to Y Oshida.

At least 73 records · Page 4Linked to original sources

Dentin bonding system. Part II: Effect of crosshead speed.

Dentin bond strengths are normally evaluated by conducting mechanical tests in tensile and/or shear mode at a certain level of crosshead speed. According to the literature review, crosshead speed is varied in a relatively wide range from 0.1 mm/min to 10.0 mm/min (with the exception of a few studies conducted above 10.0 mm/min). It was also found that crosshead speeds of 0.5, 1.0, 2.0 and 5.0 mm/min were commonly employed to evaluate dentin bond strengths for both tensile and shear modes, although no rationale for selecting the crosshead speed was described in the articles. For testing dentin bond strengths, no plastic deformation should be involved, and fracture only should take place during the fracturing process. With the Scotchbond Multiple-Purpose/Z100 bonding system, tensile bond strengths were evaluated at crosshead speeds of 0.1, 0.5, 1.0, 5.0 and 10.0 mm/min. It was found that (i) there was no strain rate sensitivity when the test was conducted below the 1.0 mm/min crosshead speed, and (ii) if the crosshead speed exceeded 1.0 mm/min, a strain rate dependency of the tensile bond strengths was observed. Knowledge that the composite resin (Z100) did not exhibit strain rate dependency influenced the conclusion that (iii) the above observed strain rate dependency was solely due to the mechanical response from the bonding interface region.

Bias↗

A clinical survey on the compliance of exercise therapy for diabetic outpatients.

To clarify the present state of exercise therapy for diabetes mellitus, we conducted a survey of 570 diabetic outpatients by written questionnaires. The results revealed that approximately 30% of the patients did not implement the prescribed exercise regimen. The principal reasons for low compliance were 'lack of time to do' and 'lack of mind to do'. 'Lack of time to do' was particularly numerous in male patients. Other reasons were 'lack of guidance by physician', 'lack of interest', 'lack of understanding of procedure'. These findings indicated that the patients should be motivated adequately when they are diagnosed as having diabetes and subsequently encouraged to reinforce their intentions by the physician and co-medical staff. Furthermore, they showed that specific approaches suitable for each individual patient and not standardized guidelines should be devised. As regards the daily environment of patients, the cooperation of the patient's family would be effective and the exercise facilities and the exercise guidance personnel should be augmented.

Diabetes Mellitus↗

Effect of daily voluntary running on in vivo insulin action in rat skeletal muscle and adipose tissue as determined by the microdialysis technique.

The effect of physical training on in vivo insulin-stimulated glucose utilization in relation to glycolysis (lactate formation) in rat peripheral tissues was investigated in 8 sedentary controls (SC) and 7 voluntary running rats (VR). We used a sequential euglycemic clamp procedure (insulin infusion rate; 6.0, 30.0 mU/kg.min) in combination with a microdialysis technique in M. quadriceps femoris, vastus lateralis, and inguinal adipose tissue. In the clamp study, glucose infusion rate (GIR) averaged over 45-75 min during the 6.0-mU/kg.min insulin infusion was significantly (p < 0.01) higher in VR (15.36 +/- 0.83 mg/kg.min, mean +/- SE) than in SC (10.41 +/- 0.88 mg/kg.min), and the lack of a significant difference in GIR between VR and SC was found during the 30.0-mU/kg.min insulin infusion. In these tissues, there was no significant difference in dialysate lactate levels between VR and SC in the basal state without insulin or glucose infusion, or at an insulin infusion rate of 30.0 mU/kg.min. However, dialysate lactate concentrations in muscle averaged over 45-75 min during the 6.0-mU/kg.min insulin clamp procedure in VR (8.51 +/- 0.71 mg/dl) were significantly (p < 0.05) higher than in SC (6.18 +/- 0.48 mg/dl). These results indicated that insulin action in skeletal muscle and adipose tissue could be evaluated in vivo by using the microdialysis technique, and that an increase in GIR in VR was, in part, explained by an increase in lactate formation in skeletal muscle.

Adipose Tissue↗

Development of photo-sensing bulge ductility tester.

The ductility of thin films is normally evaluated by conducting the bulge testing. However, the accuracy of the conventional type of mechanical bulge ductility testing unit is poor in determining the actual elongation. In this development, a photo-sensitive device is provided in the detecting portion to accurately measure the elongation. The sensitivity depends on the type of the materials tested. A Pyrex glass ball is utilized as a punching ball to provide equally distributed light. Pushing of the Pyrex glass is activated by a hydraulic media to control the punching velocity. Accuracy and reproducibility were primarily checked with copper thin foil deposited on polyimide substrates. Two types of dental articulating papers (paper-based and plastic-based) were tested. It was suggested that an appropriate selection of articulating paper to mark the occlusal interferences is essential for precise determination of occlusal harmony.

Dental Materials↗

Some mechanistic observation on water-deteriorated dental composite resins.

Four different types of dental composite resins were subjected to the water sorption environment in both dyed and undyed distilled water for 1 and 5 weeks at 37 degrees C. Weight changes due to the water sorption, effects of water sorption on mechanical properties, and fractographic observations were conducted. It was found that (i) the water sorption was diffusion-controlled, (ii) both break stress and modulus of elasticity reduced by increasing the amount of absorbed water, and (iii) fractographic observations showed that fracture pattern of the wet/dry/wet lamellar structure was not straight line, rather S-shaped pattern, suggesting that (a) mechanical properties (particularly, the modulus of elasticity) of the wet portion were not same as those in the dry portion, and (b) there could be an internal residual stress developed at/in the interfacial zone between the dry and wet portion. By one-layer removal from the wet/dry/wet lamellar structure composites, the residual stress was calculated from measuring the radius of the curvature. It was found that for all water-sorbed composite materials, tensile residual stresses were developed, which were from 30 to 40% of the break stresses for TPH, Charisma, and Durafil VS, and was 78% of the break stress for Z100 material.

Composite Resins↗

Experimental development of a chitosan-bonded beta-tricalcium phosphate bone filling paste.

Bone filling substances are needed to meet several requirements including nontoxicity, setting time, changes in pH values, and amount of dissolved elements as well as mechanical properties. In this study, the bone-generating composites were prepared by employing the in vivo absorbable beta-tricalcium phosphate as a parent matrix kneaded with CaO, MgO, and ZnO as bone mineral additives with different compositions. The setting time, pH values, compressive strength were investigated as a function of the amount of these bone mineral additives. It was found that the setting time was shortened by increasing CaO, MgO, and ZnO contents. Increasing ZnO contents resulted in the pH value lower, while the pH values increased by increasing CaO and MgO contents. Increasing ZnO contents caused the compressive strength stronger, on the other hand, the compressive strength was weakened by increasing MgO contents. Furthermore, calcium appears to be selectively released from the hardened composite sample.

Analysis of Variance↗

Fractal dimension analysis of mandibular bones: toward a morphological compatibility of implants.

In addition to biological and mechanical compatibilities for promising implant materials, a morphological compatibility is proposed by the authors. It has been reported by many investigators that implant surface with appropriate roughness and pore size exhibit better bone ingrowth activities. However, these parameters cannot characterize the complexity of surface textures. In the present study, dentulous and edentulous mandibular alveolar bones were utilized. Four segments from each mandible were subjected to the Fractal Dimension (DF) analysis. It was found that the dentulous mandible showed the DF of 1.81 +/- 0.03 while the edentulous mandible exhibited DF of 1.55 +/- 0.07, indicating that the former has more complex surface texture. It was also found that there could be a linear relationship between the surface roughness and the fractal dimension.

Alveolar Process↗

Anodic polarization behavior and microstructure of a gallium-based alloy.

A gallium-based alloy (GA) that was developed as a substitute for dental amalgam was investigated for anodic polarization behavior in deoxygenated Ringers solution, 37 degrees C. The related microstructures were examined and microanalyses were conducted. Four polarization tests were conducted by scanning from -300mV to +1,000 mV (vs. SCE) at 2 mV/s. Polarization of the first sample (GA-1) was stopped after the first anodic dissolution peak (-100 mV, 1.5-2.0 x 10(-3) A/cm2). The fourth sample (GA-4) was interrupted at the secondary peak (+1000 mV, 0.3 A/cm2). It was found that (1) the early stage of the first peak is related to selective dissolution of divalent tin ions, followed by a dissolution of Ga. Transmission electron diffraction (TED) identified the brown corrosion product as Ga2O3; (2) the GA-4 sample was covered with the white corrosion product of mainly Sn+4, identified as SnO2. In addition, the current density of the GA sample when coupled with a high-copper dental amalgam was 0.03 A/cm2 (with +1,000 mV) at the second peak which was about a ten times lower value than for the uncoupled sample; (3) the uncoupled gallium alloy and gallium alloy coupled with a high-copper dental amalgam showed 10(3)-10(4) times higher anodic current density than that of an uncoupled high-copper dental amalgam, suggesting that the gallium alloy is more corrosion prone.

Corrosion↗

Effects of adrenodemedullation on in vivo insulin-stimulated glucose utilization in relation to glycolysis in rat peripheral tissue.

The effect of adrenodemedullation (ADMX) on insulin action was examined in anesthetized rats by means of a three-step euglycemic clamp procedure (insulin infusion rate: 0, 6.0 and 30.0 mU.kgBW-1.min-1) combined with a microdialysis technique in skeletal muscle and adipose tissue. The dialysate lactate levels in the above tissues increased in parallel with the plasma lactate levels during the sequential euglycemic clamp. In the euglycemic clamp, the glucose infusion rate (GIR) was significantly (P < 0.01) higher in ADMX rats (13.41 +/- 0.82 mg.kgBW-1.min-1) than in SHAM rats (10.21 +/- 0.87 mg.kgBW-1.min-1) during the 6.0-mU.kgBW-1.min-1 insulin infusion, and the lack of a significant difference between ADMX and SHAM rats was observed during the 30-mU.kgBW-1.min-1 insulin infusion. In skeletal muscle, the concentration of lactate in dialysate was significantly higher in ADMX rats (9.29 +/- 1.01 mg/dl) than in SHAM rats (6.22 +/- 0.47 mg/dl) (P < 0.05) at an insulin infusion rate of 6.0mU.kgBW-1.min-1. In adipose tissue, no significant difference in dialysate lactate levels was found between ADMX and SHAM rats at any insulin infusion rate. These results suggest that 1) it is possible to determine insulin action in skeletal muscle and adipose tissue in vivo by using the microdialysis technique, that 2) ADMX appears to result in a significant increase in insulin sensitivity, and that 3) lactate formation increased in skeletal muscle, but not in adipose tissue.

Adipose Tissue↗

Fractal dimension analysis of aluminum oxide particle for sandblasting dental use.

Aluminum oxide particles are commonly used as a sandblasting media, particularly in dentistry, for multiple purposes including divesting the casting investment materials and increasing effective surface area for enhancing the mechanical retention strengths of succeedingly applied fired porcelain or luting cements. Usually fine aluminum oxide particles are recycled within the sandblasting machine. Ceramics such as aluminum oxides are brittle, therefore, some portions of recycling aluminum oxide particles might be brittle fractured. If fractured sandblasting particles are involved in the recycling media, it might result in irregularity metallic materials surface as well as the recycling sandblasting media itself be contaminated. Hence, it is necessary from both clinical and practical reasons to monitor the particle conditions in terms of size/shape and effectiveness of sandblasting, so that sandblasting dental prostheses can be fabricated in optimum and acceptable conditions. In the present study, the effect of recycling aluminum oxide particles on the surface texture of metallic materials was evaluated by Fractal Dimension Analysis (FDA). Every week the alumina powder was sampled and analyzed for weight fraction and contaminants. Surface texture of sandblasted standard samples was also characterized by FDA. Results indicate very little change in particle size, while the fractal dimension increased. Fractal dimension analysis showed that the aluminum oxide particle as a sandblasting media should be replaced after 30 or 40 min of total accumulated operation time.

Air Pressure↗

Titanium-porcelain system. Part I: Oxidation kinetics of nitrided pure titanium, simulated to porcelain firing process.

The bonding strength of porcelains to metals depends on the oxide layer between the porcelain and the metal. Oxidation of a metal surface increases the bonding strength, whereas excessive oxidation decreases it. Titanium and its alloys are gaining acceptance for dental use since they exhibit excellent biocompatibility, corrosion resistance, low specific gravity, good mechanical properties, and low cost. However, titanium suffers from its violent reactivity with oxygen at high temperatures that yields an excessive thick layer of TiO2, and this presents difficulties with porcelain bonding. The present study deals with the oxidation kinetics of titanium simulated to porcelain firing and evaluating surface nitridation of titanium as a process of controlling the oxidation behavior of titanium. Nitrided samples with the Arc Ion Plating PVD process and un-nitrided control commercially pure titanium (CPT, Grade 1) were subjected to oxidation simulating firing of Procera porcelain with 550 degrees, 700 degrees, and 800 degrees C firing temperatures for 10 min in both 1 and 0.1 atmospheric air. Weight difference before and after oxidation was calculated and the parabolic rate constant, Kp (mg2/cm4/s), was plotted against inverse absolute temperature. Surface layers of the samples were subjected to x-ray and electron diffraction techniques for phase identifications. Results revealed that both nitrided and un-nitrided samples obey a parabolic rate law with activation energy of 50 kcal/mol. In addition this study shows that nitrided CPT had a Kp about 5 times lower than the un-nitrided CPT and hence the former needs about 2.24 times longer oxidation time to show the same degree of oxidation. Phase identification resulted in confirming the presence of TiO2 as the oxide film in both groups but with 1-2 microns thickness for the un-nitrided CPT and 0.3-0.5 micron thickness for the nitrided samples. Therefore it can be concluded that nitridation of titanium surface can be effective in controlling the surface oxide thickness that might ensure satisfactory bonding with porcelain.

Crystallography↗

Influence of sympatho-adrenal system on insulin sensitivity using the euglycemic clamp technique.

The aim of the present study was to further investigate the role of the adrenergic system on insulin action using the euglycemic clamp technique. Whole-body glucose metabolism (GM) was calculated as the glucose infusion rate for maintaining euglycemia under insulin infusion and used as an indicator of insulin sensitivity. Euglycemic clamps were performed in adrenodemedullated, epinephrine-treated, phentolamine-treated (alpha-blockade), propranolol-treated (beta-blockade), and epinephrine plus phentolamine and/or propranolol-treated rats. The following results were obtained at an insulin level of approximately 80 mU/l. GM in adrenodemedullated rats (13.97 +/- 0.98 mg/kg/min) was significantly higher than that of the control rats (10.26 +/- 0.50 mg/kg/min, P less than 0.01). GM in epinephrine-treated rats (1.7 mg/kg body weight/h) was 2.12 +/- 0.49 mg/kg/min (P less than 0.001 vs. control). Dose-response curves for phentolamine and propranolol established maximally effective doses (3.0 mg and 12 mg/kg body weight/h, respectively). Using these doses, GM in epinephrine plus phentolamine-treated rats (4.90 +/- 0.39 mg/kg/min) was significantly higher than that of epinephrine alone and GM in epinephrine and propranolol-treated rats (4.49 +/- 0.47 mg/kg/min) was also significantly higher than that of the epinephrine alone. GM in the epinephrine plus both propranolol and phentolamine (5.94 +/- 0.45 mg/kg/min) was significantly higher than that of the epinephrine alone, but not different from either treatment alone and was not additive. Neither phentolamine alone (9.48 +/- 1.45 mg/kg/min), propranolol alone (10.36 +/- 0.55 mg/kg/min) or the combination of blockades (11.14 +/- 0.65 mg/kg/min) had any effect on GM.(ABSTRACT TRUNCATED AT 250 WORDS)

Adrenal Medulla↗

Effects of daily physical activity on insulin action in the elderly.

The effects of daily physical activity on peripheral insulin action were investigated in aged individuals. Glucose infusion rates (GIR) during the euglycemic insulin clamp procedure in aged bedridden, aged controls, and aged athletes were compared with those in young controls and young athletes at insulin infusion rates of 40 and 400 mU.m-2.min-1 to estimate insulin action at physiological and maximal insulin concentrations, respectively. At both insulin infusion rates, GIR was significantly higher in aged athletes and significantly lower in aged bedridden subjects than in aged controls. Although there was no statistical difference in GIR at 400 mU.m-2 x min-1 between young athletes and young controls, GIR at 40 mU.m-2 x min-1 was higher in young athletes than in young controls. Comparison of the aged and young groups showed that although GIR at 400 mU.m-2 x min-1 was significantly lower in aged controls than in young controls, there was no significant difference between the aged athletes and the young athletes. We conclude that insulin responsiveness (insulin action at the postreceptor binding site) may decrease with the aging process and may be further affected by physical inactivity. Although physical training may improve insulin responsiveness in aged individuals up to levels similar to those in young athletes, physical training in young individuals may improve only insulin sensitivity.

Adult↗

Microanalytical characterization and surface modification of TiNi orthodontic archwires.

Orthodontic archwires (equiatomic TiNi alloy) of both used (4 weeks) and unused conditions were microanalyzed by optical and scanning electron microscopes, energy dispersive X-ray spectroscopy, and electron diffraction to characterize the surface layers. They were also subjected to immersion and polarization corrosion tests in a 0.9% NaCl aqueous solution. Based on results obtained from these analytical and experimental studies, surfaces of TiNi archwires were further electrochemically treated to etch away nickel selectively and reform the surface morphology to uniform and porous surface layers. Main conclusions were: (a) surface layers of used archwires were covered contaminants causing the discoloration, and the contaminants were identified as mainly KCl crystals, (b) surfaces of both used and unused wires were observed to be irregular features characterized by lengthy island-like structures, where nickel was selectively dissolved, (c) corrosion tests in a 0.9% NaCl aqueous solution in immersion and polarization methods indicated that by increasing temperature from 3 degrees to 60 degrees C and acidity from pH 11 to pH 3, calculated corrosion rates increased, and (d) surface layers of TiNi archwires can be electrochemically modified to selectively etch nickel away, leaving a Ti-enriched surface layer and forming a uniformly distributed porous surface that may reduce the coefficient of friction against the orthodontic brackets.

Corrosion↗

Effects of training and training cessation on insulin action.

Physical training has been shown to improve glucose tolerance and insulin action. In the present study, insulin action was determined using the euglycemic clamp technique in six trained male athletes compared with six untrained controls matched by age, sex, and weight at 14, 38, and 86 hours and at 6 days after cessation of exercise. The rate of insulin-mediated glucose uptake (glucose disposal) was 9.40 +/- 0.46 mg.kg-1.min-1 (mean +/- SEM) for the athletes at 14 h after the last exercise bout, compared with 6.80 +/- 0.86 mg.kg-1.min-1 obtained for the untrained controls (p less than 0.01). Glucose disposal was gradually decreased to 7.78 +/- 0.87 mg.kg-1.min-1 at 38 h, 6.82 +/- 0.49 mg.kg-1.min-1 at 86 h and to 7.11 +/- 1.00 mg.kg-1.min-1 at 6 days after cessation of physical training. At 38 h, 86 h, and 6 days of detraining, glucose disposal exhibited by training athletes did not differ significantly from untrained controls. These results suggest that physical training increases insulin action, and that this effect could be reversed to the control levels within 38 h after detraining.

Adolescent↗

[In vivo insulin sensitivity in adrenodemedullated rats].

Insulin sensitivity and responsiveness were determined in adrenal demedullated rats (ADMX) with euglycemic insulin clamp technique. Adrenal medulla was extirpated bilaterally a week before the study. Catheters were placed at right atrium via right jugular vein for sampling blood and at inferior vena cava via femoral vein for the infusion of insulin and glucose solution. Insulin was infused at rates of 4.4, 8.8, 14.7, 29.3, 88.0, 293.0 mU/kg/min. Blood was collected every five min. during the clamp and glucose infusion rate was modulated to control the blood glucose concentrations at fasting levels. Glucose metabolism was calculated from the amount of glucose infused from 60th to 120th min. during the euglycemic clamp. The results obtained were as follows: 1. Glucose metabolisms of ADMX in each infusion rate of insulin, 4.4, 8.8, 14.7, 29.3, 88.0, 293.0 mU/kg/min were 5.2 +/- 0.5, 12.5 +/- 0.5, 17.6 +/- 1.2, 19.8 +/- 2.3, 29.0 +/- 1.5, and 25.2 +/- 1.9 mg/kg/min, respectively. 2. Glucose metabolisms of control group in each dose were 6.6 +/- 0.4, 9.0 +/- 0.9, 18.5 +/- 1.2, 23.4 +/- 2.4, 24.6 +/- 1.1, and 27.0 +/- 1.3 mg/kg/min, respectively. 3. Significant difference (p less than 0.01) in glucose metabolism between ADMX and control was observed at the insulin infusion rate of 8.8 mU/kg/min which might be equivalent to physiological hyperinsulinemia. 4. There were not any differences in insulin responsiveness between both groups. These results suggest that epinephrine regulates insulin sensitivity under physiological hyperinsulinemic condition via defects of insulin receptors.

Adrenal Medulla↗

[Effect of aging and physical inactivity on glucose tolerance and insulin sensitivity].

It has been well documented that glucose intolerance is associated with aging, but it is not yet clear whether this phenomenon is due to the aging process itself or is secondary to the appearance of other age-related conditions among which physical inactivity is one of most important variables. To evaluate the effect of aging process and/or physical inactivity on insulin action, this study was undertaken using the euglycemic insulin clamp technique and the oral glucose tolerance test (OGTT). Subjects without diabetes mellitus and other serious diseases consisted of 14 non-obese aged individuals and 10 young controls (YC group), ranging in age from 63 to 85 yrs, and from 19 to 21 yrs, respectively. The aged individuals were further divided into two groups (one was termed as the AS group, in which 7 aged subjects had been confined to bed for at least 3 months and the other was termed as the AC group in which 7 aged controls kept their daily physical activity such as walking). The results of OGTT did not show any remarkable differences between AC and YC groups. In the AS group, however, glucose intolerance and low insulin response during OGTT were observed. In view of the tissue insulin action, MCR, which is thought as a reliable marker for tissue insulin action, evaluated by euglycemic insulin clamp was 5.31 +/- 0.68, 8.57 +/- 1.20, 9.60 +/- 0.35 ml/kg/min in the AS, AC and YC groups, respectively (AS less than AC, p less than 0.05, AS less than YC, p less than 0.01, AC less than YCM, N.S.).(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗