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Biomedical subjects

Y Oka

Publications and source records attributed to Y Oka.

At least 541 records · Page 30Linked to original sources
PubMed1983

Kinetic study on disappearance of gamma-butyrolactone-gamma-carbonyl-L-histidyl-L-prolinamide (DN-1417) from plasma using a radioimmunoassay for DN-1417 isobutylamide.

Kinetic study of disappearance of gamma-butyrolactone-gamma-carbonyl-L-histidyl-L-prolinamide (DN-1417) from human plasma has been performed by a sensitive and specific radioimmunoassay (RIA) using antiserum against DN-1417 isobutylamide. Antibody against N-[2-hydroxy-4-(isobutylcarbamoyl)butyryl]-L-histidyl-L-prolinamide (DN-isobutylamide) did not cross-react with any other TRH-related peptides tested. DN-isobutylamide was radioiodinated with 125I by the chloramine T method and the product had a specific activity of about 160 microCi/microgram. The sensitivity of the RIA was 50 pg per tube, and the intra- and inter-assay coefficients of variation at concentrations of 1-10 ng/ml were 7.0-11.6%. DN-1417 in the plasma was converted to a stable form, DN-isobutylamide, by incubation with isopropanol-isobutylamine at room temperature for 2 h. DN-Isobutylamide was separated from 2-hydroxy-4-carboxybutyryl-L-histidyl-L-prolinamide (DN-COOH) using a SEP-PAKTM C18 cartridge before RIa. The recovery of DN-isobutylamide from plasma was 86.2-98.2%. The half-life, volume of distribution and plasma clearance rate of DN-1417 examined in 6 normal male volunteers by intravenous injection of 500 micrograms DN-1417 citrate were 11.5 +/- 5.6 min in the first phase and 89.5 +/- 33.5 min in the second phase, 25.3 +/- 10.0 liters and 745.8 +/- 278.6 1/d, respectively. These results indicate that RIA of DN-isobutylamide is useful for analysing DN-1417 metabolism in humans.

Adolescent↗
PubMed1983

Medical treatment of infective endocarditis and its limitations.

In an attempt to establish an appropriate management program for patients with active infective endocarditis, 30 patients treated during the past 20 years were studied retrospectively. Microorganisms were confirmed in 27 of the 30 patients (90%) (86.7% by culture and 3.3% at surgery). Among these 27 patients, viridans streptococci were confirmed in 23 (85.2%), aureus staphylococci in 2 (7.4%), epidermidis staphylococci in one (3.7%) and candida albicans in one (3.7%). Twenty of the 23 patients (87%) with viridans streptococcal endocarditis were treated medically with good success, but 2 patients (8.7%), who developed severe congestive heart failure (NYHA 3-4 degrees), underwent emergency surgery, and one of them (4.3%) died from severe heart failure. The synergistic effects of the combined antibiotic therapy were not ascertained in the present series. It was noteworthy that a bolus intravenous administration of penicillin was more effective than its continuous drip infusion. Both patients with aureus staphylococcal endocarditis died before surgery. One patient with prosthetic valve endocarditis due to candida died after a second operation. It is concluded that most patients with active infective endocarditis can be medically treated successfully, but surgery should be performed urgently for patients with severe heart failure, poor response to antibiotics, aureus staphylococcal and candida endocarditis and prosthetic valve endocarditis.

Adolescent↗
PubMed1983

The relationship of microvesicles to the plasmalemma of rat adipocytes.

Ruthenium red binding demonstrates that the extensive microvesicle system of isolated rat adipocytes is, for the most part, open to and continuous with the plasmalemma proper. Morphometric estimates indicate that insulin treatment has no effect on the relationship between microvesicles and the cell surface. Neither does insulin affect the apparent lack of pinocytotic activity of these vesicles as judged by a time course analysis of cells incubated with horseradish peroxidase, which is bound to the membrane of the vesicles, but is not internalized. Insulin does produce a small but repeatable and measurable increase in average diameter of the microvesicles from 73 to 78 nm. Unlike the positively charged ruthenium red, which binds to both plasmalemmal as well as microvesicular surfaces, cationic ferritin did not readily bind to microvesicle membranes, a result indicating some distinction between the two membrane surfaces. The implications of the lack of dramatic visible morphological effects of insulin upon the adipocyte plasmalemma and it's associated microvesicles are discussed in light of the proposed role of insulin as a mediater of translocation of membrane-associated transporters to and from the cytoplasm.

Adipose Tissue↗
PubMed1983

[Coexisting malignant lymphoma and early cancer of the stomach].

A 62-year-old man with malignant lymphoma and early gastric cancer as two independent growths in the stomach was reported. In the resected stomach, malignant lymphoma was observed as a protruding lesion measuring 5.5 X 3.0 X 1.2 cm with four giant folds at the anterior wall of the upper gastric body. There also was a poorly differentiated adenocarcinoma as an early gastric cancer of type IIc + III; it measured 0.9 X 0.7 cm and invaded the submucosal layer at the antral portion. There were no pathological findings, excepting intestinal metaplasia, in the mucosal layer between the two lesions. There was no metastasis from either the malignant lymphoma or the adenocarcinoma to perigastric lymph nodes.

Adenocarcinoma↗