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Biomedical subjects

Y Ohtsuki

Publications and source records attributed to Y Ohtsuki.

At least 325 records · Page 18Linked to original sources
PubMed1983

Immunoagglutination of type-C virus particles in a human T-cell line by serum supplementation from patients with adult T-cell leukemia.

A T-cell line, MT-2, derived from human cord blood lymphocytes by cocultivation with adult T-cell leukemia (ATL) cells is a continuous producer of type-C virus particles. Electron microscopy of MT-2 cells cultured for 1-3 weeks in medium containing 10% ATL patients' sera revealed agglutination of type-C virus particles within the electron-dense deposits in the extracellular spaces. No such agglutination occurred in control cultures supplemented with normal human or fetal calf serum. These results provide direct evidence for the specific reactivity of ATL patient's sera with type-C virus particles in the MT-2 cell line at the ultrastructural level.

Agglutination↗
PubMed1983

Detection of antibodies to adult T-cell leukemia (ATL)-associated virus in ATL patients' sera by immunoelectron microscopy.

In an attempt to demonstrate the presence of antibodies to ATL-associated type-C virus particles (ATLV), an indirect immunoferritin method of immunoelectron microscopy was performed on an ATLV-producing human cord T-cell line (MT-2) and short-term cultures of ATL cells. All five sera from ATL patients seropositive to ATL-associated antigens (ATLA) but not three sera from healthy adults were positive for the ferritin labeling of ATLV and plasma membranes in both MT-2 and short-term cultured ATL cells. Sera absorbed with sheep red blood cells or human T-cell acute lymphatic leukemia cells labeled ATLV much more intensely with ferritin than with plasma membranes. These results demonstrated at the ultrastructural level that anti-ATLA-positive sera contained antibodies to surface glycoproteins and/or structural proteins of ATLV and that they differed from anti-Forssman or anti-T-cell antibodies. It was also demonstrated that anti-ATLA-positive sera showed the same reactivity with ATLV from both MT-2 and short-term cultured ATL-cells by the immunoferritin method.

Antibodies, Viral↗
PubMed1983

Plasma cell granuloma of the stomach combined with gastric cancer.

An autopsy case of plasma cell granuloma of the stomach combined with gastric cancer is reported. Plasma cell granuloma presumably occurred independently of adenocarcinoma in the stomach, and nodular or diffuse infiltration of plasma cell containing many Russell bodies was observed in the gastric wall, intermingled with lymphocytes. Infiltrating plasma cells were polyclonal in nature as seen by peculiar meshwork patterns of positive immunofluorescence against human gamma, kappa, and lambda chains. Ultrastructurally, globular Russell bodies, 1-7 micrometers in diameter, were located in the rough endoplasmic reticulum and could be divided into two types, smooth-or rough-surfaced one.

Adenocarcinoma↗
PubMed1983

Transformation of rabbit lymphocytes with T-cell leukemia virus.

Co-cultivation of rabbit lymphocytes with a human cell line carrying adult T-cell leukemia (ATL) virus (ATLV) resulted in the establishment of an ATLV-producer rabbit cell line. This cell line expressed ATL-associated antigens and reacted with antisera to the structural proteins of human T-cell leukemia virus which is identical or closely related to ATLV.

Animals↗
PubMed1983

[Establishment and characterization of ATLV-producing cell lines].

Peripheral blood lymphocytes from a Japanese monkey (Macaca fuscata) were co-cultivated with lethally irradiated MT-2 cells that carry abundant type C virus particles (ATLV) isolated from a patient with adult T-cell leukemia (ATL). After six weeks, a lymphoid cell line designated Si-1 was established from the simian lymphocytes. The Si-1 line was E-, SIg-, Leu-1-, OKI1+, EBNA-, ATLA+ and ATLV+. Co-cultivation of peripheral blood lymphocytes from three anti-ATLA positive and two negative healthy adults resulted in the establishment of three lymphoid cell lines derived either from an anti-ATLA positive donor or both donors. All three cell lines were E+, SIg-, Leu-1+, OKI1+, EBNA-, ATLA+ and ATLV+. This mixed lymphocyte culture technique provides a simple means for the isolation of ATLV from healthy ATLV carriers.

Adult↗
PubMed1983

[Case of primary infiltrating carcinoma in the right breast with metastatic ovarian dysgerminoma].

A 33-year-old woman whose left ovarian dysgerminoma had been resected presented with left axillar swelling and a right breast tumor about three years after surgery. Histological examination of the mastectomized tumor revealed primary infiltrating carcinoma into the muscle layer and a metastatic ovarian dysgerminoma showing well-defined proliferation. Despite chemotherapy, she died 7 months later of breast cancer. To our knowledge, this is the only reported case of its kind in the world literature.

Adult↗
PubMed1983

[Case report of lipoleiomyoma of the uterus].

A 64-year-old woman with a lipoleiomyoma of the uterus is reported. Histologically, the tumor was predominantly composed of mature fatty tissue and intermingled with irregular-shaped fibromuscular tissue. The fibromuscular tissue showed mast cell infiltration. Ultrastructural study revealed the smooth muscle component to have irregularly indented nuclei, there were cytoplasmic fusiform dense patches, discontinuous external lamina, and occasional fat vacuoles in the nuclei and cytoplasma. The histogenesis of this tumor is most likely metaplasia of the fibromuscular component of fatty tissue.

Female↗
PubMed1982

Malignant hyperthermia.

A report is made of a 65-year-old male who died of a malignant hyperthermia of 42 degrees C. Symptoms included muscle rigidity at the termination of operation for neck-clipping of an aneurysm of the anterior communicating artery. Latent myopathy was observed in skeletal muscle, and a bleeding focus was present in the left anterior region of the hypothalamus, coinciding with the temperature regulation center. The mechanism of onset of this disease is still poorly understood, but it seems that this disease occurred due to synergic effects of the hypothalamic hemorrhage and the pre-existing myopathy. The serum CPK level at the time of death was abnormally high (250 U), and 3.4% of isozyme CPK1 was detected. The serum myoglobin was 204, 850 ng/ml, a markedly high level, and myoglobinuric nephrosis was present as a result.

Aged↗