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Biomedical subjects

Y Ohtsuki

Publications and source records attributed to Y Ohtsuki.

At least 307 records · Page 17Linked to original sources
PubMed1985

Nodular alteration of the paracortical area. An in situ immunohistochemical analysis of primary, secondary, and tertiary T-nodules.

With the use of in situ immunohistochemical techniques on freshly frozen and paraffin-embedded material from 63 reactive lymph nodes, the cellular composition of T-nodules observed in 30 cases with nodular alteration of the paracortical area was analyzed. T-nodules were composed of S-100 beta + interdigitating reticulum cells (IDRCs), variable numbers of OKT6+ dendritic cells (DCs), high endothelial venules, and a very high T helper/T suppressor ratio because of an enrichment of OKT4+, Leu3a+ helper/inducer T cells in these nodules. According to their localization in the paracortical area, and the arrangement of IDRCs and high endothelial venules, T-nodules could be divided into "primary" and "secondary" T nodules. In all cases of dermatopathic lymphadenitis, very large aggregates of S-100 beta + and OKT6+ DCs, admixed with few high endothelial venules and variable numbers of OKT4+, Leu3a+ helper/inducer T cells, were observed and were termed "tertiary T-nodules." It is suggested that T-nodules represent the paracortical counterparts of B-lymphoid follicles and are the in vivo equivalents of DC/T-cell clusters observed in vitro. According to their cellular composition and localization in the lymph node, "primary" and "secondary" T-nodules probably represent subsequent maturation stages of distinctive nodular paracortical structures, which play an important role in the presentation of antigens to helper/inducer T cells and in the proliferation of antigen-responsive T cells. Their close topographic relationship to B-lymphoid follicles may indicate their role in the extrafollicular generation of antibody-forming cells.

Antibodies, Monoclonal↗
PubMed1985

Experimental infection of rabbits with human T-cell leukemia virus type I.

Rabbits were successfully infected with human T-cell leukemia virus type I (HTLV-I) and produced antibodies to adult T-cell leukemia-associated antigens (ATLA) on intravenous inoculation of the HTLV-producing human cord T-cell line MT-2, or autologous cell lines established by cocultivation with MT-2 cells. Lymphocytes taken from the rabbits between 4 and 14 days after the inoculation of MT-2 cells, but not lymphocytes obtained in earlier or later periods, could be immortalized in vitro and expressed ATLA and type C virus particles. However, lymphocytes harvested during an early culture period (5 to 8 days) were found to be negative for ATLA. The transformed cells had the karyotype of a normal male rabbit. Two rabbits inoculated with the autologous HTLV-producing cell lines did not allow their growth in vivo, but some peripheral blood lymphocytes could be immortalized in vitro. One of these transformed cell lines was examined for the integration of HTLV-I provirus genome. The transformed cells were found to contain the provirus genome and also to be monoclonal with respect to the integration site of the provirus genome, unlike the inoculated cells.

Animals↗
PubMed1985

[A case of stromal tumor of the testis with peculiar crystalloid structures].

A case of poorly differentiated stromal tumor occurring in the left testis of a 37-year-old man is reported. Microscopically, the tumor was composed of polygonal cells arranged in a sheet-like pattern. The ill-defined cytoplasm contained disease-resistant PAS positive granules. Ultrastructurally, peculiar crystalloid structures were found in the cytoplasm of the tumor cells appearing in tubular, beaded or lamellar form and connected with the nuclear membrane as well as with the rough endoplasmic reticulum. These are somewhat similar to annulate lamellae. Interdigitation of the plasma membranes and zonula adherens was observed. Basal laminae were recognized in parts. These findings suggest that this tumor is a case of poorly differentiated gonadal stromal tumor showing a tendency to differentiate into Sertoli's cells.

Adult↗
PubMed1985

[A case of lobular carcinoma of the breast containing endocrine granules].

A case of lobular carcinoma containing many endocrine granules in the breast of a 76-year-old woman is reported. Histologically, tumor cells proliferated in solid or nest-like patterns featuring lobular carcinoma. Although argyrophilic and immunohistochemical reactions for peptide hormones were negative, electron microscopic observation revealed endocrine granules, 200-400 nm in diameter, associated with a limiting membrane and a halo in every tumor cell examined. It was suggested that this case might be considered a variant of lobular carcinoma and that breast cancer showing lobular patterns should be searched for the possibility of endocrine differentiation.

Aged↗
PubMed1984

Heterotransplantation and maturation of a chronic myelogenous leukemia cell line (KCL-22) in vivo.

Heterotransplantation was performed to study the maturational capacity of a new Ph1 chromosome-positive chronic myelogenous leukemia cell line (KCL-22). Five million KCL-22 cells of undifferentiated blasts were implanted intraperitoneally into 15 immunosuppressed newborn hamsters. Of the 15 hamsters, 10 that survived developed granulocytic tumors 16-61 days after implantation. The tumor cells were composed of a mixed population of undifferentiated blasts, neutrophils, and eosinophils and had a human female karyotype with double Ph1 chromosomes identical to that found in the KCL-22 cell line used for transplantation. These findings indicate that Ph1 chromosome-positive cells can be induced to mature along the neutrophil-eosinophil lineage after growth in the heterologous hosts.

Animals↗
PubMed1984

Immunohistochemical study on the distribution of alpha and beta subunits of S-100 protein in human neoplasm and normal tissues.

The immunohistochemical distribution and localization of the alpha and beta subunits of S-100 protein in human neoplasms and normal tissues were studied by the PAP method using monospecific rabbit antibodies against each subunit. Beta subunit immunoreactivity was detected in all S-100-positive cells and tumors reported previously. In contrast alpha subunit immunoreactivity was absent from Schwann cells, schwannomas, neurofibromas, granular cell myoblastomas, pituicytes of the neurohypophysis, Langerhans cells, interdigitating reticulum cells, and histiocytosis X cells. Interestingly, only the alpha subunit was detected in neurons of both central and peripheral nervous system, and in lymph node macrophages. Human S-100-positive cells are divided into three groups; the first is composed of cells containing only the beta subunit (probably S-100b; beta beta), the second consists of cells containing both the alpha and beta subunits, and the third is composed of cells containing only the alpha subunit (probably S- 100ao ; alpha alpha). The ontogentic relationships between S-100-positive cells and tumors are discussed in the light of these findings.

Biomarkers↗
PubMed1984

Granulocytic sarcoma presenting as a mediastinal tumor. Report of a case and cytological and cytochemical studies of tumor cells in vivo and in vitro.

An unusual case of granulocytic sarcoma in a 23-year-old man is reported. The patient initially presented with mediastinal tumor and was diagnosed clinically as having thymoma. The patient was treated by radiotherapy and surgical removal of the tumor. Histology of the excised tumor had been nondiagnostic because of extensive fibrous changes. Eight months later, the patient developed pleural effusion on the right, which soon was followed by blood and bone marrow pictures consistent with acute promyelocytic leukemia. In vitro culture of pleural effusion cells unexpectedly gave rise to a continuously growing peroxidase-positive myeloid cell line. Autopsy revealed the recurrent mediastinal tumor to be positive for intracytoplasmic naphthol AS-D chloroacetate esterase and lysozyme activity. From these findings, the patient retrospectively was diagnosed as having mediastinal granulocytic sarcoma, which terminated in pleural effusion and acute promyelocytic leukemia.

Adult↗
PubMed1984

Ultrastructural study of type C virus particles in phytohemagglutinin-stimulated lymphocytes from healthy adults seropositive to adult T-cell leukemia-associated antigens.

PHA-stimulated peripheral lymphocytes from six healthy adults consisting of four family members of an adult T-cell leukemia (ATL) patient and two blood bank donors, seropositive to ATL-associated antigens (ATLA), were all positive for the expression of ATLA and ATL-associated virus (ATLV). These results revealed that anti-ATLA-positive persons were healthy carriers of ATLV and that the numbers of ATLV observed in each culture were more proportional to the percentage of ATLA-positive lymphocytes than anti-ATLA titers of each person. Virus particles detected were C-type in morphology, and essentially similar to those observed in MT-2 and other ATL-related cells, but rather uniform in size, mostly 120-130 nm in diameter. Furthermore, it is of interest that tubuloreticular structures and striated fusiform structures, which were found in fresh or short term cultured ATL cells, were observed in some cultures of lymphocytes from anti-ATLA-positive healthy persons.

Adolescent↗
PubMed1984

Detection of HLA-D-region-associated antigens on the surface of adult T-cell leukemia virus particles by immunoelectron microscopy.

To search for lymphocyte marker antigens on the surface of human T-cell leukemia virus (HTLV), an immunoelectron microscopic study was performed on a HTLV-producing human T-cell line, MT-2, using monoclonal antibodies, such as anti-Leu-1, -Leu-2b, -Leu-3a, -Leu-5, -Leu-10 and -HLA-DR and OKIal. The reactivity of each antibody with MT-2 cells was tested by the immunoperoxidase method at the light microscopic level. OKIal, anti-HLA-DR and -Leu-10 gave positive results. At the ultrastructural level, the surface of HTLV as well as the plasma membranes of MT-2 cells were labeled with ferritin by the monoclonal antibodies OKIal, anti-HLA-DR and -Leu-10, but not by anti-Leu-1 and -Leu-3a. These findings suggest that HLA-D region -associated antigens are common antigenic determinants shared by the surface of HTLV and the plasma membranes of MT-2 cells. These antigens on the virus surface are probably picked up selectively from the plasma membranes and may play an important role in the interaction of HTLV and target T-cells.

Antibodies, Monoclonal↗
PubMed1984

Infectious transmission of human T-cell leukemia virus to animals.

An human T-cell leukemia virus (HTLV)-producing cell line (Ra-1) was established from rabbit lymphocytes by co-cultivation with lethally irradiated MT-2 cells. Ra-1 cells were inoculated intravenously into a Japanese monkey and rabbits. All animals responded with the production of antibodies to HTLV. Lymphocytes from the seroconverted animals were grown in the presence of T-cell growth factor (TCGF) or co-cultured with lymphocytes from seronegative healthy persons. The TCGF-grown cells, which were chromosomally of the recipient type, expressed HTLV antigens and particles. The co-cultures gave rise to human T-cell lines which also harbored HTLV antigens and particles. Blood transfusion from the infected rabbits resulted in the seroconversion of the recipient rabbits. HTLV-producing lymphoid cell lines were established from some of the transfused rabbits. The recipient origin of these cell lines was determined by chromosome analysis. It was possible to serially transmit HTLV by blood transfusion in rabbits. Thus, these animals offer promise as a laboratory model for HTLV infection.

Animals↗
PubMed1984

Establishment of a new peroxidase-positive human myeloid cell line, PL-21.

A myeloid cell line, designated PL-21, was established from the peripheral blood of a patient with acute promyelocytic leukemia. The PL-21 cell line grew in single-cell suspension, with a doubling time of 48-64 hr, and consisted of promyelocytes with fine immature nuclei and prominent azurophilic granules in the cytoplasm. PL-21 cells were positive for peroxidase, naphthol AS-D chloroacetate esterase, and Sudan Black B staining. Under the usual culture conditions, a small proportion of these cells differentiated into mature granulocytes, and this differentiation was enhanced by the addition of dimethyl sulfoxide in the culture medium. PL-21 cells had receptors for the Fc portion of IgG and complement, intracytoplasmic lysozyme and phagocytic activity, but lacked Epstein-Barr virus-associated nuclear antigen. Chromosome analysis of this cell line revealed a human male polyploid karyotype with 13q+ and double minute chromosomes. This new myeloid cell line may provide useful material for the study of proliferation and differentiation of human leukemia cells.

Adult↗
PubMed1984

Immunohistochemical localization and distribution of S-100 proteins in the human lymphoreticular system.

Immunohistochemical localization of S-100b protein and S-100ao protein in human lymphoreticular system was studied by using monospecific antibody directed against either the alpha subunit or beta subunit of S-100 protein. S-100b protein immunoreactivity was detected in Langerhans cells, interdigitating reticulum cells, and histiocytosis X cells, but not in ordinary macrophages and blood monocytes. In contrast, S-100ao protein immunoreactivity was detected in blood monocytes, macrophages of lymph node, alveolar macrophages of lung, and small numbers of Kupffer cells of liver. S-100ao immunoreactivity was also detected in epithelioid cells, Langhans giant cells, and foreign body giant cells. The present findings suggest that the presence of S-100ao protein in the cytoplasm is one of the characteristic features of cells in the human mononuclear phagocyte system. The detection of S-100b, but not S-100ao, immunoreactivity in Langerhans cells and interdigitating reticulum cells also suggests that they are independent of the monocyte-macrophage system. S-100ao protein may be a novel cytoplasmic marker for cells of the human monocyte-macrophage system.

Biomarkers↗
PubMed1984

[A case of localized lipomatosis in the pancreatic head presenting a lipoma-like appearance].

A 63-year-old woman with localized lipomatosis in the pancreatic head is reported. She was diagnosed to have a lipoma by clinical data including CT scanning. Histopathological examination of the extirpated mass revealed that the lesion was mostly composed of mature fatty tissue without any lobulation or encapsulation by connective tissue, there was a small amount of scattered pancreatic parenchyma at the peripheral and deeper parts of the mass. No Langerhans' islets were detected. Based on these findings, the lesion was diagnosed as localized lipomatosis in the pancreatic head.

Female↗