Biomedical subjects
Publications and source records attributed to Y Nose.
A study of HLA-D clusters associated with DR4 was performed in the Japanese population. These clusters consist of DYT, DKT2, DB3, and Dw4. Forty-two Japanese typed as DR4 were investigated, and it was found that 17 (40.5%) were DYT, 7 (16.7%) DKT2, 7 (16.7%) DB3, and 4 (9.5%) Dw4.
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The reliability of telephone electrocardiogram (ECG) transmission facilities was assessed in order to carry out the computerized ECG analysis using the IBM-Bonner program. Simulated ECGs were transmitted repeatedly over telephones from an internal laboratory and from an external hospital which was 1,000 Km distant. The simulated ECG was transmitted in a highly reproducible state and with no appreciable distortion. The telephone ECG transmission facilities using the public telephone network in Japan proved to reliable for computerized ECG analysis.
Using an indirect enzyme-labeled antibody method, localization of SP1, HPL and HCG in spontaneous abortions (29 cases) was compared with those in induced abortions (39 cases). At the same time, histological changes were examined in the same specimens. 1) Histologically, the fibrinoid degeneration in cytotrophoblastic mass was more often found in spontaneous abortions than in induced abortions. Stromal fibrosis frequently existed in spontaneous abortions (6-10 weeks: 53%, 11-16 weeks: 93%). Stromal edema was rare in induced abortions, but was detected in spontaneous abortions (6-10 weeks: 13%, 11-16 weeks: 50%). These findings were increased with gestational age. 2) Horseradish peroxidase (HRP) reaction products from SP1, HPL and HCG located mainly in the syncytiotrophoblast. The intensity of HRP reaction was not always uniform in each villus. 8% in induced abortions and 59% in spontaneous abortions showed reaction with anti-HCG in some stromas in syncytiotrophoblast. Most of them also showed stromal fibrosis. In spontaneous abortions, the intensity of HRP reaction on HPL was relatively increased in comparison with that on HCG or SP1. In some severe degenerated specimens, SP1 and HPL were found in syncytiotrophoblast, but HCG seemed to be located on the outer part of the stroma adjacent to syncytiotrophoblast.
We have developed a system of extracorporeal circulation that removes proteins of the molecular weight of the circulating immune complexes of rheumatoid arthritis by cryogelation with hollow-fiber membrane filtration. A 52-year-old woman with a 36-year history of severe, unremitting, high-titer, seropositive rheumatoid arthritis who had failed to respond to anti-inflammatory, antirheumatic, and cytotoxic drugs was chosen for a trial of this system. A rapid and sustained decrease in circulating immune complexes as measured by C1q binding occurred, accompanied by a much slower improvement in clinical factors of disease activity. Rheumatoid factor changed very little and loss of other serum proteins by the procedure was relatively modest. This new procedure was successful in removing circulating immune complexes in a patient with rheumatoid arthritis, and in inducing a remission in one who has not had such in 36 years, while sparing volume and other plasma proteins.
We developed a highly sensitive EIA for determination of SP1. The detection limit was 5ng/ml, and the correlation of EIA and RIA was extremely good (SAWAKI et al., 1980). Using this method, we found that SP1 was detected even in the serum at 12 days of ovulation. The concentration of SP1 in cord blood was on average 127ng/ml (24-540ng/ml). SP1 in newborn serum was 126ng/ml (22-355ng/ml) in 1st, and almost undetected in 4th day. SP1 in urine was 9.7ng/ml in the 5th week of pregnancy, 1,210ng/ml at peak in the 32th week, and in after delivery was 439ng/ml in 1st day and afterwards descended to 6ng/ml in 8th day. In 2 infertile women SP1 was detected in the sera of 14 days after ovulation. This suggests that SP1 measurment might be useful in the diagnosis of occult pregnancy. Three patients with throphoblastic disease were found to have regression curves for both HCG and SP1. The sera of 39 patients with uterine cervical carcinoma were examined to determine the occurrence of elevated SP1 levels. Ten of 27 patients before treatment had increased SP1 levels while only one of 12 patients had an increased SP1 level after treatment.
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Immune response to Candida allergen in man was studied using the lymphocyte reaction in vitro and the skin test in vivo. One hundred and ten normal healthy Japanese were typed for HLA-A, B, D and DR. It was found that HLA-B7, Dw1 and DR1 were strongly associated with high responsiveness and Den and Mt2 with low responsiveness. In a family study, the inheritance of high responsiveness was observed. In the reaction patterns of HLA-D homozygote and heterozygote cells, it was found that Dw1/DR1 and Dw12/DR2 were associated with a high responders and that DYT/DR4 and DEn (6.1)/DR- were associated with low responders. It is considered that the Dw1/DRl gene might be more dominant as an immune response; the Ir gene when compared to a gene such as the DYT, and the EDn (6.1) as an immune suppressive; Is gene. A genetic factor can be implicated in the secondary response (but not the primary response) to Candida allergen. Therefore, the existence of HLA-linked Ir and/or Is gene to Candida allergen is discussed.
The properties of pregnancy-specific beta 1-glycoprotein (SP1) were examined in urines of pregnant women. Two cross-reactive substances with rabbit antisera to SP1 were observed in the urines of pregnant woman. By agar gel immunodiffusion method two lines were formed and by single radial immunodiffusion (SRID) double rings were detected in concentrated pregnancy urines. The immunoprecipitate of the inner ring in SRID was distinct, while that of the outer ring was rather indistinct, compared with the former. The substance to make inner ring (tentatively called SP1-A) was found to be the same as SP1 in pregnant serum. The SP1-like substance to make outer ring (tentatively called SP1-B) appeared to be specific to pregnant urine. Although the precipitation lines of urinary SP1 were slightly different from that of serum SP1, both Sp1-A and SP1-B were migrated towards the beta 1-globulin region in immunoelectrophoresis. The molecular weights were estimated by gel filtration method and calculated to be 110,000 for SP1-A and 75,000 for SP1-B, respectively.
Activation of naturally sensitized human T lymphocytes to Candida allergen was studied using three HLA-D and DR heterozygote Japanese cells (Dw1.DR1/DYT.DR4, Dw12.DR2/DYT.DR4, DYT.DR4/DEn.DR blank) and four HLA-D and DR homozygote cells (Dw1.DR1, Dw12.DR2, DYT.DR4, DEn.DR blank). In vitro activation of T lymphocytes to Candida allergen was found to require the presence of autologous or allogeneic compatible HLA-Dw1.DR1 and Dw12.DR2 macrophages.
Patients with uncontrollable pruritus secondary to cholestatic liver disease were subjected to plasma perfusion in an attempt to remove the toxins responsible for this symptom. The improvement in the degree of pruritus was dramatic and surprisingly long-lasting in five of six patients. One patient had a response more difficult to evaluate. Serum bile acids levels fell in all patients in whom pruritus improved but not in the patient who responded less favorably.
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In order to measure beta1-SP1-glycoprotein (SP1), on RIA system by the 2-antibody method was established as a measuring method of the low concentration range. SP1 concentrations were measured by this method in the sera of women in early pregnancy, in the amniotic fluids of late pregnancy, in the sera of malignant tumour patients. Furthermore, SP1 concentrations in the sera of women in early pregnancy as well as E2, E3, progesterone and HCG concentrations in the same samples were measured, and correlations between them were examined. 1) The minimum sensitivity of this measuring system was 3ng/ml. The optimum concentration of samples was between 10 approximately 660ng/ml. 2) The correlation between the data obtained by this RIA method and the SRID method was as close as r = 0.9287. 3) SP1 concentrations in the sera of women in early pregnancy were 0.17 +/- 0.12 microgram/ml in the fifth week of pregnancy, showing an almost straight increase during the course of pregnancy, and were 31.62 +/- 3.20 microgram/ml in the 13th week of pregnancy. 4) SP1 concentrations in amniotic fluids were 1.09 approximately 2.20 microgram/ml and were equal to about 1% of SP1 concentrations in the sera of women in late pregnancy. SP1 concentrations in the sera of umbilical cord blood were 0.13 approximately microgram/ml, which were equal to about 0.1% of SP1 concentrations in the sera of women in late pregnancy. 5) SP1 was detected in all four samples of the choriocarcinoma patients' sera, and the concentrations were 25 approximately 2,600ng/ml. Sp1 was detected in 6 of the 15 samples of the cervical carcinoma patients' sera, and the detection rate was 40%. SP1 was detected in 3 of the 6 samples of the leukemia patients' sera and 1 of the 4 samples of the prostatic cancer patients' sera. SP1 detection rates and concentrations appeared to increase in the sera of cervical carcinoma and leukemia patients in accordance with the progress of the diseases. SP1 concentrations in the sera of women in early pregnancy were not correlative to measured E2 and E3 concentrations in the same samples, but there was a significant correlation with progesterone and HCG concentrations. The RIA method for measuring SP1, which we have established, is available for measuring SP1 in the low concentration range. The method is expected to be applied clinically, such as in examinations in early pregnancy, and will be available in fundamental studies such as attempts to measure the SP1 movement in malignant tumour patients, in cooperation with studies of the meaning of SP1 production at pregnancy.
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