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Biomedical subjects

Y Noguchi

Publications and source records attributed to Y Noguchi.

548 records · Page 31Linked to original sources
PubMedDate not supplied

Generation of cytotoxic T lymphocytes against autologous lung cancer cells resistant to apoptosis.

BACKGROUND: [corrected] Non-small cell lung cancer (NSCLC) is resistant to conventional treatment; so the development of a new therapy is urgent. MATERIALS AND METHODS: 50 patients with NSCLC and malignant effusion were enrolled in this study. Seventeen autologous lung cancer cell lines were established. Peripheral lymphocytes and irradiated autologous tumor cell lines were co-cultured to generate cytotoxic T lymphocytes (CTL). Expression of apoptosis-related molecules were analysed by RT-PCR or FACS. RESULTS: CTL lines were established in 2 patients. Both CTL lines were CD3+, CD8+ and MHC class I-restricted T cells and showed cytotoxic activities not only against autologous tumor cell lines but against allogenic cancer cell lines. Two lung cancer cell lines were established from one patient before and after cisplatin-based chemotherapy. The tumor cell line established after chemotherapy was apoptosis-resistant, but was sensitive to cytotoxicity of CTL. CONCLUSION: CTL-based immunotherapy may be one of the candidates for future therapies against NSCLC.

Adult↗
PubMedDate not supplied

Primary thyroid lymphoma: evaluation with US, CT, and MRI.

OBJECTIVE: Our goal was to determine the most effective radiologic workup in diagnosing and staging primary thyroid lymphomas and the value of knowing the extent of tumors for prognostic purposes. MATERIALS AND METHODS: Magnetic resonance imaging, CT, US, and fine-needle aspiration (FNA) biopsy (palpation- or US-guided) findings were retrospectively reviewed in 14 patients with primary thyroid lymphomas (5 palpable, 9 nonpalpable). Survival was correlated with the presence or absence of extrathyroidal spread of lymphoma. RESULTS: Thyroid lymphomas were detected in all patients with US and 12 (86%) each with CT and MRI. Histopathologic diagnosis was obtained with FNA in 11 patients (79%) and with surgical procedures in 3. Magnetic resonance imaging correctly diagnosed tumor involvement of the lymph nodes (four of four patients), muscle (five of five), esophagus (one of one), internal jugular vein (one of one), and carotid artery (one of one). Muscle invasion was missed with CT in one patient. Ultrasound was not accurate for diagnosing tumor invasion of the esophagus, carotid artery, and lymph nodes. The survival of patients without extrathyroidal spread was significantly (p < 0.05) better than that of patients (n = 5) with extrathyroidal spread. CONCLUSION: Palpable thyroid masses should undergo palpation-guided FNA for diagnosis and nonpalpable masses US-guided FNA. Tumor extent and staging should be determined with MRI, which will contribute to initiating appropriate therapy and prediction of prognosis.

Adult↗
PubMedDate not supplied

Predictive value of c-erbB-2 and DNA ploidy patterns in gastric carcinoma recurrence.

This study was conducted to see whether the accuracy in predicting liver metastases in gastric cancer patients increased when DNA aneuploidy and c-erbB-2 gene products were analyzed besides other clinicopathologic features. Aneuploidy was observed in 32 out of 74 tumors (43%); these included 62% of those tumors in patients with liver metastases, 43% of those with peritoneal dissemination and 26% of those in the 5-year survival group. Aneuploidy was positively correlated with the prognosis and degree of blood vessel invasion but not with lymph node metastases or lymphatic permeation. Positive staining for the c-erbB-2 gene product was detected in 19 out of 89 tumors (21%). This staining was not related to any of the clinicopathologic features examined. Multivariate analysis revealed that the degree of blood vessel invasion was the factor that most strongly correlated with liver metastases. The prognostic value of aneuploidy was significant (p < 0.05) but much smaller than that of blood vessel invasion (p < 0.0001), interstitial tissue reaction (p = 0.02), Borrmann type classification (p = 0.03). Analyses of DNA aneuploidy and c-erbB-2 gene expression in the primary tumor of gastric carcinoma to improve the accuracy of predicting liver metastasis seem to be of limited clinical value at present.

Aneuploidy↗
PubMedDate not supplied

Suppression of thrombin formation during hemodialysis with triglyceride.

Effect of blood-air contact in the venous line air-trap chamber on blood clotting was studied. Fourteen chronic hemodialysis patients (six men, eight women; mean age, 49 years) with elevation of thrombin-antithrombin III were studied. To prevent blood-air contact, triglyceride (NOF-005) was floated over the blood in the chamber. Control hemodialysis was performed for 4 weeks and hemodialysis using NOF-005 followed for the next 4 weeks. Clot formation in the circuit was examined after each hemodialysis and clotting factors including thrombin-antithrombin III, FXII Antigen, vWF Antigen, PF4 and beta-thromboglobulin were measured before and after the last hemodialysis of each control and NOF-005 hemodialysis. Clotting in the chamber was improved when NOF-005 was used. Thrombin-antithrombin III increase during hemodialysis was suppressed to about 30% of control values by using NOF-005. blood-air contact seems to promote thrombin generation and accelerate clot formation.

Adult↗
PubMedDate not supplied

Alimentary hyperglycemia in patients with subtotal gastrectomy is associated with decreased insulin secretion but not with insulin resistance.

BACKGROUND: It is not clear whether alimentary hyperglycemia in gastrectomized patients is related with glucose tolerance or not. METHODS: Glucose tolerance, insulin secretion, and peripheral insulin sensitivity were evaluated in 36 patients who underwent subtotal gastrectomy for gastric cancer using the oral glucose tolerance test (OGTT), the intravenous glucose tolerance test (IVGTT) and the euglycemic hyperinsulinemic glucose clamp technique (Glucose clamp). RESULTS: Patients were categorized into three groups by OGTT; 8 cases were in group I (normal), 19 cases in group II (alimentary hyperglycemia), and 9 cases in group III (glucose intolerance). Both glucose disappearance rate (K value expressed as, %/min) and insulinogenic index as determined by IVGTT were significantly lower in groups II (1.00 +/- 0.24, 0.21 +/- 0.18, respectively) and III (0.90 +/- 0.13, 0.11 +/- 0.14, respectively), compared with group I (1.58 +/- 0.71, 0.35 +/- 0.18, respectively). Peripheral insulin sensitivity as measured by glucose clamp was not decreased in groups II and III. CONCLUSIONS: These results suggest that in patients with alimentary hyperglycemia, glucose tolerance was impaired due to a decreased pancreatic sensitivity to blood glucose and not due to insulin resistance.

Aged↗
PubMedDate not supplied

Plasma interleukin-6 is not a mediator of changes in lipoprotein lipase activity in cancer patients.

BACKGROUND/AIMS: Cancer cachexia is characterized by a variety of metabolic disorders. Alterations in fat metabolism have been reported to be associated with suppression of tissue lipoprotein lipase (LPL) activity in tumor-bearing animals. Interleukin-6 (IL- 6) has been documented to reduce tissue LPL activity and may play a role in inducing cancer cachexia. This study was conducted to clarify the changes in LPL activity and the role of IL-6 in patients with either gastrointestinal cancer or breast cancer. METHODOLOGY: Twelve patients with colorectal cancer, 7 patients with gastric cancer, 7 patients with breast cancer and 5 normal volunteers were studied. Serum concentrations of triglycerides (TG), non-esterified fatty acids (NEFA) and IL-6 were measured. LPL activity was measured in plasma post-heparin administration. The relationships of LPL activity to tumor progression, body weight loss and serum IL-6 levels were examined. The effect of tumor resection on LPL activity was also studied. RESULTS: LPL activity was suppressed with tumor progression in patients with either gastrointestinal cancer or breast cancer. Suppression of LPL activity and the degree of weight loss were negatively correlated in patients with either gastric or colorectal cancer (r = -0.5826, p = 0.011) but not in patients with breast cancer. The decrease in LPL activity was not always reversed after resection of the tumor. Circulating IL-6 did not correlate with either plasma LPL activity or tumor progression. CONCLUSIONS: Reduced LPL activity in patients with advanced gastrointestinal or breast cancer may reflect changes in nutritional status. Serum IL-6 is less likely to be a mediator of these alterations.

Adult↗
PubMedDate not supplied

Clinicopathologic differences between gastric remnant cancer and primary cancer in the upper third of the stomach.

BACKGROUND: The prognosis of gastric remnant cancer is considered to be poor compared with that of primary cancer. MATERIALS AND METHODS: This study was conducted to elucidate the differences of clinicopathologic findings and treatment results between 59 patients with gastric remnant cancer (GRC) and 579 patients with primary proximal gastric cancer (PGC). RESULTS: There was no significant difference in the incidence of lymph node metastasis between GRC and PGC. However, changes in the metastatic pattern to lymph nodes were observed in GRC. In GRC, the tumor easily invaded the neighboring organs due to the adhesions around the remnant stomach, resulting in a low resectability with curative intent. The 5-year survival rate after curative resection for advanced GRC was 50.9%. GRC patients without serosal invasion had a good prognosis. CONCLUSION: Although the survival rate after curative resection for GRC patients was similar to that of PGC, GRC patients without serosal invasion had a better prognosis. Therefore, early detection is an important way to improve overall survival in GRC.

Adult↗