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Y Nishibe

Publications and source records attributed to Y Nishibe.

At least 37 records · Page 2Linked to original sources
PubMed1989

Isolation of Inoue-Melnick virus from human colorectal carcinoma cell lines.

Inoue-Melnick virus (IMV) was isolated from five of six human colorectal carcinoma cell lines, whereas the virus was not isolated from three normal colon-derived cell lines, three pancreas carcinoma cell lines, two bladder carcinoma cell lines, one kidney adenocarcinoma cell line, and one cervical carcinoma cell line. IMV synthesis was remarkably enhanced in the colorectal carcinoma cell lines by treatment with 5-bromodeoxyuridine (BUDR). However, virus was not detected in any of the other cell lines in spite of BUDR treatment. Five strains of virus isolated from colorectal carcinoma cell lines were subsequently identified by the neutralization test as intermediate-type IMV.

Bromodeoxyuridine↗
PubMed1989

Species and sex differences in the inhibitory action of the corticosteroid alclometasone dipropionate on the hepatic drug-metabolizing system.

The effect of successive administration of the corticosteroid alclometasone dipropionate (ACM) on the hepatic drug-metabolizing system was examined using male and female rats. Although some pharmacological changes such as increases in plasma enzyme activity, lipid level and protein concentration appeared similarly in ACM-treated male and female rats, the activities of 7-alkoxycoumarin O-dealkylase, especially the O-depropylation activity, decreased dose-dependently by ACM administration only in male rats. ACM did not affect the hepatic drug-metabolizing activity in female rats and mice of both sexes. Also, ACM did not inhibit androgen-independent aniline hydroxylase activity even in male rats. The time course of changes of the drug-metabolizing system in male rats showed a rapid decrease in cytochrome P-450 content and O-depropylation activity following successive treatments with ACM, but there was a slow onset in the decreases of the O-demethylation and O-deethylation activities of 7-alkoxycoumarin. When ACM was withdrawn, the O-demethylation and O-deethylation activities rapidly returned to their control levels, while recovery of the O-depropylation activity was slow. These results suggested that ACM inhibits the hepatic drug-metabolizing enzyme activity associated with a specific form(s) of androgen-dependent cytochrome P-450 in male rats.

Animals↗
PubMed1987

Isolation of Inoue-Melnick virus from human meningioma-derived cell cultures and detection of antibody in patients with meningioma.

Inoue-Melnick virus (IMV) was isolated from six of seven human meningioma-derived cell cultures, while the virus was not isolated from six other brain tumor cell cultures. Sera of 145 consecutive neurosurgical inpatients were tested for IMV-neutralizing antibody. Of 26 patients with meningioma, 22 were positive for IMV antibody (84.6%). Of the remaining 119 patients, 16 were positive.

Antibodies, Viral↗
PubMed1987

Endogenous production of infectious Inoue-Melnick virus in a human meningioma cell line.

We investigated continuous production of Inoue-Melnick virus (IMV) in the MG-1 cell line, established from human meningioma. The infectious virus, identified as a type 1 virus, was mostly recovered extracellularly. Assay of MG-1 cells as infective centers indicated that most of the cells were capable of producing infectious virus. By immunofluorescence, more than 90% of the cells were found to have IMV-associated cytoplasmic antigen(s) (IMCA).

Cell Line↗
PubMed1987

Factors influencing the assay of Inoue-Melnick virus.

We describe factors influencing the assay of Inoue-Melnick virus (IMV) and practical comments for its assay. MRC-5 cells at a population doubling level (PDL) of more than 32 were more sensitive than younger cells for demonstrating cytopathic effect (CPE) of the virus. The PDL of MRC-5 cells also influenced the virus yield, particularly when the amount of viral inoculum was small. Cells at younger PDL produced less virus. The appearance of CPE and the virus yield were inhibited by increasing the concentration of NaHCO3 to 0.14% in the maintenance medium. The release of the virus into the medium was also inhibited as NaHCO3 was increased.

Bicarbonates↗
PubMed1987

Antibody survey for Inoue-Melnick virus infections in Osaka and Houston.

Neutralizing antibodies to the Inoue-Melnick virus were detected in healthy adults in Osaka and in Houston. For adults, the prevalence rate for the cross-reacting type 3 virus was 13.8 per cent in Osaka and 29.1 per cent in Houston. Antibodies were also found in immune serum globulin prepared from American adults, but not in sera of Osaka children under 10 years of age. The Inoue-Melnick virus isolates so far obtained in Houston could be classified into three antigenic types, but in Japan all have been type 1. Type 3 antibody tests detect all homotypic infections plus about 82 per cent of type 1 infections. Thus, the actual prevalence rates for type 1 infections in Japan are somewhat higher than those found with the type 3 assay, namely, about 14 per cent for young adults and 24 per cent for those over 50 years of age. Further studies are needed to elucidate the natural history of this virus infection.

Adolescent↗
PubMed1987

Cross-reacting antibodies to human T cell leukemia virus-I and -II in Japanese patients with hairy cell leukemia.

Sera from 11 Japanese patients with hairy cell leukemia and 4B122, an anti-hairy cell serum produced by the authors, were surveyed by Western blot analyses for cross-reacting antibodies to human T cell leukemia virus (HTLV)-I and -II virions released, respectively, from Lma-66 and Mo-T cell lines. Sera from the majority of the patients showed positive reactions with p15, p19, and/or p24 of HTLV-I and with p21 and/or p22 or HTLV-II. 4B122 was weakly cross-reactive with HTLV-II but not with HTLV-I. These results militate against the involvement of HTLV-I or -II but may implicate a role by a cross-reacting, previously unrecognized retrovirus in the pathogenesis of hairy cell leukemia in Japanese patients.

Antibodies, Viral↗
PubMed1986

Inhibitory effect of acyclovir on the growth of Inoue-Melnick virus isolated from cerebrospinal fluid.

Inoue-Melnick virus (IMV) was isolated from the cerebrospinal fluid of patients with chronic neurologic disorders. The effect of acyclovir (ACV), 9-(2-hydroxyethoxymethyl)-guanine, on the growth of IMV was studied in human diploid cell cultures. ACV markedly inhibited the multiplication of all three IMV serotypes in vitro with the same inhibitory effect. By passage of IMV in the presence of ACV in the medium, ACV-resistant mutants of three IMV serotypes were easily isolated.

Acyclovir↗
PubMed1986

Thermostabilization of Inoue-Melnick virus by salt.

Inoue-Melnick virus (IMV) was stabilized to thermal inactivation at 50 degrees C by 1 M Na2SO4 but not by 1 M MgCl2, 1 M MgSO4, 2 M NaCl, and 2 M KCl. The latter salts enhanced the inactivation over that in distilled water. Na2SO4 stabilized IMV at 1 M concentration but enhanced inactivation at 0.1 M. Three types of IMV were stabilized by 1 M Na2SO4 at 50 degrees C to almost the same degree. The pattern of the thermostabilization of IMV by salts was similar to that of herpes simplex virus; however, the degree of its stabilization was lower with prolonged heating.

Herpesviridae↗
PubMed1986

A comparative study on the effects of disulfiram and beta-lactam antibiotics on the acetaldehyde-metabolizing system in rats.

Several beta-lactam antibiotics, especially those containing N-methyltetrazolylthiomethyl groups at the 3-position of the cephalosporin nucleus, affect the alcohol-metabolizing system in rats. These effects were compared those with disulfiram, well-known as a potent inhibitor of aldehyde dehydrogenase (ALDH). Both disulfiram and antibiotics containing the N-methyltetrazolylthiomethyl group inhibited both mitochondrial low Km ALDH and acetaldehyde oxidation in rat livers. The high Km ALDH and alcohol dehydrogenase activities in livers were not affected by these treatments. When ethanol was given to rats pretreated with disulfiram or these antibiotics, the blood acetaldehyde concentration increased markedly concomitant with a decrease in activity of the low Km ALDH. Administration of N-methyltetrazolethiol alone suppressed the low Km enzyme activity and also increased the blood acetaldehyde level; both effects were pronounced and observed several hours after administration. beta-Lactam antibiotics without N-methyltetrazolethiol in their molecule did not affect the liver mitochondrial enzyme activity or the blood acetaldehyde level.

Acetaldehyde↗
PubMed1985

Inhibitory effect of glucocorticoid hormones on the growth of Inoue-Melnick virus.

Glucocorticoid hormones inhibited the early stage of propagation of Inoue-Melnick virus in a dose-dependent manner in human diploid cell cultures. Prednisolone and hydrocortisone achieved complete inhibition of replication of the three Inoue-Melnick virus serotypes at concentrations of 0.2 X 10(-4) and 10(-5) M, respectively; strong inhibition was found even at 10(-5) and 10(-6) M, respectively. The steroid hormones did not show a direct effect on the virus.

Cell Line↗
PubMed1984

Classification of Inoue-Melnick virus into three antigenic types.

Antigenic analysis of Inoue-Melnick virus, formerly called SMON virus, showed that isolates from patients with subacute myelo-optico-neuropathy (SMON) in Japan, thus far tested, belonged to a single antigenic type (type 1). However, Inoue-Melnick virus isolates obtained so far in the United States chiefly from patients with multiple sclerosis could be classified into three types: type 1, type 2, and an intermediate type. At the present state of knowledge, the virus has not been proven to be the causative agent of disease, hence its provisional designation as IMV.

Antigens, Viral↗
PubMed1982

SMON and MS.

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Diagnosis, Differential↗
PubMed1982

SMON and MS.

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Diagnosis, Differential↗
PubMed1982

Isolation of virus from the spinal fluid of three patients with multiple sclerosis and one with amyotrophic lateral sclerosis.

A transmissible agent with the properties of a thermolabile filterable virus was isolated from the cerebrospinal fluid (CSF) of 4 Houston patients with chronic central nervous system (CNS) disease. 3 had multiple sclerosis and I had amyotrophic lateral sclerosis. No isolations were made from 27 patients whose CSF was taken for diagnosis of illness unrelated to degenerative CNS disease. Neutralising antibodies to the 4 isolates were present in the serum of each of the 4 patients with virus (14 positive results among 15 tests) but in hardly any of the sera from 8 patients without virus (3 antibody-positive results among 32 tests). The agent is antigenically related to the virus isolated in Japan from cases of subacute myelo-optico-neuropathy.

Adult↗
PubMed1982

Differential effects of sulpiride and metoclopramide on brain. Homovanillic acid levels and shuttle box avoidance after systemic and intracerebral administration.

Sulpiride (SUL) is more efficacious in antipsychotic activity than metoclopramide (MET), although both appear to possess almost equal affinities for dopamine (DA) receptors. We studied the effects of SUL and MET on the brain homovanillic acid (HVA) level and shuttle box avoidance to clarify their differential modes of action on the blockade of DA receptors. SUL was several times less potent than MET, which was about 50 times less potent than the control drug haloperidol (HAL), for increasing HVA at 4 h after s.c. administration. However, SUL was about 50 times more potent than MET and about equipotent to HAL, at 2 h after drug administration into the lateral ventricle. The potency of SUL by intraventricular administration was 20-fold or more than that by intracisternal administration. HAL and MET did not show such a discrepancy. The HVA level reached the maximum 1 h after intraventricular administration of MET or HAL, while s.c., intracisternal or intraventricular administration of SUL caused a gradual increase in the HVA level with the maximum being reached after 6-8 h. In shuttle box avoidance, MET was about 25 times more potent than SUL by intraperitoneal administration but about 200 times less potent than SUL, which was about 10 times more potent than HAL, by intraventricular administration. These results suggest that SUL causes a potent, long-lasting blockade of DA receptors, while MET has only a weak, short-lasting action. These findings may be related to the differences in their clinical efficacy.

Animals↗