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Biomedical subjects

Y Nagano

Publications and source records attributed to Y Nagano.

At least 145 records · Page 8Linked to original sources
PubMed1986

[Successful chemotherapy in postoperative brain metastasis of breast cancer using ACNU--two case reports].

Two cases of postoperative brain metastasis of breast cancer were evaluated after chemotherapy using ACNU. Case 1: A 47-year-old female, who had undergone right standard radical mastectomy in 1979 for breast cancer (T2 N0M0, papillo-tubular carcinoma), was treated with ADM, TAM, and 60Co irradiation for bone metastasis in 1983. In 1984, she complained of loss of consciousness and paralysis of the extremities due to brain metastasis. After chemotherapy using ACNU (100 mg X 3), brain metastasis could not be detected on CT. She remained asymptomatic for more than 9 months without recurrence after therapy. Case 2: A 46-year-old-female, who had undergone left standard radical mastectomy in 1980 for breast cancer (T1 N1 M0, medullar tubular carcinoma), complained of headache and vertigo accompanying a hard tumor in the scalp. Chest X-ray and CT demonstrated right lung metastasis and left cerebellar metastasis. After combination chemotherapy using ACNU (100 mg) + MMC (4 mg) i.v. and FT (600 mg/day) p.o., symptoms and tumor on CT disappeared for 10 months after therapy. However, the patient died of aggravation of angina pectoris and D.M. from which she had been suffering for several years previously. These two cases correspond to complete response (CR) according to the response criteria proposed by Koyama-Saitoh.

Antineoplastic Agents↗
PubMed1985

Dissemination of gentamicin-resistant plasmids among strains of Enterobacteriaceae.

The frequency of gentamicin-resistance among strains of many species of Enterobacteriaceae isolated from in-patients in our hospital has increased considerably since 1979. To clarify the reason for this increase, we tried to detect R-plasmids conferring gentamicin-resistance in all of the isolates in 1981 and found that most of them had conjugative R-plasmids. For epidemiological purposes we classified these into types I to X and 'others' by the following criteria: the nature of the resistances conferred; their incompatibility group; their molecular weight and the pattern of the DNA fragments after digestion with restriction endonucleasis. In the urology department, type-I plasmids conferring resistance to gentamicin, kanamycin, tobramycin and amikacin, and type-II plasmids conferring resistance to gentamicin, kanamycin and tobramycin were detected in the isolates belonging respectively to six species and twelve biotypes, and four species and six biotypes. In the second surgery ward, isolates belonging to six species and twelve biotypes were found to have type-III plasmids conferring resistance to gentamicin, kanamycin, tobramycin and chloramphenicol. Our investigation shows that not only antibiotic-resistant strains but also R-plasmids may be disseminated in hospitals.

Conjugation, Genetic↗
PubMed1985

Stored urine as a reservoir of gentamicin-resistant bacteria and as a site of conjugative R-plasmid transfer.

For some years we have observed the dissemination of gentamicin-resistant strains of Enterobacteriaceae in our hospital and we have reported the spread of antibiotic resistance by plasmid transfer. Stored urine was suspected to be a reservoir for gentamicin-resistant enterobacteria and to be a means of dissemination of gentamicin resistance amongst different species. We now report an epidemiological analysis based on biotyping of isolates and R-plasmid typing, and show that dissemination of bacterial strains and R-plasmids was not associated with disposable urine reservoirs, but dissemination of strains and plasmids was observed when non-disposable urine bottles were used in the wards.

Bacteriological Techniques↗
PubMed1984

Differentiation in mouse leukemia cells mediated by an immune interferon preparation.

Antiviral and cell-differentiating (CD) activities of immune interferon (IFN-gamma) preparation were investigated. Male STD-ddY mice were sensitized with BCG. Three weeks later, they were challenged with BCG or purified protein derivative (PPD), and exsanguinated at intervals. The sera were assayed for antiviral and CD activities. The CD activity appeared and reached the maximum earlier than the antiviral activity. The CD activity was thermostable, whereas the antiviral activity was thermolabile. By dialysis at pH 2 for 24 h, the CD activity was reduced to 15%, while the antiviral titer was completely lost. Lipopolysaccharide (LPS) given 24 h before the challenge has markedly suppressed the appearance of antiviral activity following the challenge, but did not so much affect the CD activity. The challenge with PPD stimulated greater antiviral activity and poorer CD activity than the challenge with BCG did. These findings suggest that IFN-gamma preparations contain a CD factor(s) besides IFN.

Animals↗
PubMed1984

Disappearance of terminal deoxynucleotidyltransferase in human malignant T-lymphoblasts treated with a human alpha interferon preparation.

Human T-lymphoblastoid cell lines RPMI 8402, MOLT-3, and CCRF-CEM were treated with interferon (IFN) to determine if the treatment would result in the disappearance of cellular terminaldeoxynucleotidyltransferase (TdT), a possible differentiation marker for T-lymphocytes. Incubation of RPMI 8402 cells in the presence of IFN preparation caused a decrease in the number of TdT-positive cells and in TdT activity of the cell extract. The inhibition of cell multiplication was dose dependent. The anticellular effect of IFN preparation was cytostatic, not cytocidal. The IFN preparation modified neither the TdT content nor proliferation of MOLT-3 and CCRF-CEM cell lines. The effects of IFN preparation thus varied with the cell line.

Cell Division↗
PubMed1984

Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis.

A rapid and simple plasmid isolation procedure was developed for the epidemiological analysis of plasmid-mediated antimicrobial resistance. By this method, plasmid DNAs ranging in molecular weight between 2.0 and 122 X 10(6) could be detected. Various bacteria, such as strains of the family Enterobacteriaceae, Pseudomonas aeruginosa, Haemophilus influenzae, and Staphylococcus aureus, could be analyzed. The plasmid DNA obtained could be directly used for restriction endonuclease analysis without further purification. In addition, this method made it possible to analyze several cultures at the same time.

DNA Restriction Enzymes↗
PubMed1982

Inhibitory effect of polynucleotides on solid tumor produced in mice by Ehrlich ascites cancer cells.

Solid tumors produced in mice by Ehrlich ascites cancer cells were completely regressed by treatment with the copolymer of polyriboinosinic acid-polyribocytidylic acid (poly (I):(C)) when 200 microgram of this compound was intravenously injected 3 times a week for 3-4 weeks, starting 7-8 days after transplantation of the tumor. Although the first injection of poly (I):(C) caused hyporeactivity to re-induction of IFN, the subsequent 6 doses stimulated the formation of IFN at 3685 units/ml on average after each injection, strongly suggesting that IFN plays a role in the antitumoral effect of poly (I):(C).

Animals↗
PubMed1980

Altered erythrocyte spectrin extractability in Duchenne muscular dystrophy.

Spectrin extractability was measured in the erythrocyte membranes obtained from patients with Duchenne muscular dystrophy (DMD) and normal matched controls. After the extraction of spectrin by adding deionized water, an aliquot of the pellet was applied to 6% SDS-polyacrylamide gels. Spectrin-Band 3 ratio on the gels was used as an indicator of spectrin extractability. Our data disclosed that spectrin extractability is significantly reduced in DMD patients when compared with that of controls and these results suggest that spectrin from DMD patients is more tightly bound to the membranes. Our data may also be applicable to the discussion of the relationship of spectrin to other membrane properties as well as to previously described membrane abnormalities in DMD erythrocytes.

Electrophoresis, Polyacrylamide Gel↗
PubMed1980

Enhanced production of virus-inhibiting factor (interferon) in human diploid cells by ultraviolet irradiation and temperature shift-down after stimulation with Newcastle disease virus.

The production of the virus-inhibiting factor or interferon (IF) was highest in cells incubated at 37 C after inoculation with Newcastle disease (ND) virus and decreased as the incubation temperature was lowered. Shift-down of incubation temperature to 32 C or 34 C after incubation at 37 C for 4--7 hr enhanced IF production in cell cultures stimulated with ND virus, as compared with cultures incubated continuously at 37 C. Shift-down to 32 C after incubation at 37 C for 6 hr was optimal for this enhancement of IF yield. Enhanced IF production was also observed in cell cultures irradiated by ultraviolet light 4--7 hr after stimulation with ND virus.

Cell Line↗
PubMed1980

[Role of virus inhibitory factor or interferon in the antitumoral effect of whole-body hyperthermia].

The interferon inducing effect of hyperthermia was studied in normal and tumor-bearing mice. Circulating interferon was temporarily detected one day after subcutaneous transplantation of 1.6 X 10(6) Ehrlich's ascites tumor cells. Hyperthermia of 43.5 degrees C for 5 min did not induce the interferon formation in mice with or without subcutaneous tumor of the cells. These findings showed that the induction of interferon formation was not main cause of the hyperthermia-induced tumor inhibition.

Animals↗