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Biomedical subjects

Y Mizoguchi

Publications and source records attributed to Y Mizoguchi.

At least 271 records · Page 15Linked to original sources
PubMed1981

Partial purification of the cholestatic factor derived from the lymphocytes of tuberculin-sensitized guinea pigs.

When lymph node lymphocytes from the tuberculin-sensitized guinea pigs were stimulated in vitro with PPD (purified protein derivatives) and the culture fluid was injected into the mesenteric vein of rats, a marked reduction of bile flow was observed. These culture supernatants contained cholestatic activity were fractionated by gel filtration using a Sephadex G-75 column followed by DEAE-cellulose column chromatography. Both the cholestatic activity and the macrophage migration inhibitory factor (MIF) activity were detected predominantly in the fourth fraction of gel filtration. By further fractionation using a DEAE-cellulose column chromatography, the cholestatic activity was separated into two fractions; one of them was shown to have both cholestatic activity and MIF activity, but the other did not have any detectable MIF activity. These results suggest that the cholestatic factor may be different at least partially from the MIF.

Animals↗
PubMed1981

Studies on intrahepatic cholestatic: intrahepatic cholestasis induced in rats by the culture supernatant of activated lymphocytes.

When lymph node lymphocytes from sensitized guinea pigs were stimulated with a specific antigen in vitro, and their culture supernatants injected into the mesenteric vein of rats, a marked reduction of bile flow and bile secretion was seen. Gel filtration of this active principle revealed that the active material has a molecular size similar to that of the macrophage migration inhibitory factor (MIF). Histologically, dilated bile canaliculi with decreased microvilli were observed by electron microscopy in rat liver after injection of culture supernatant. No such change was observed in rats after injection of the supernatant of lymphocyte cultures prepared from non-sensitized guinea pigs. These results strongly suggest that the sensitized lymphocytes produce a factor (or factors) which causes intrahepatic cholestasis when stimulated with a specific antigen.

Animals↗
PubMed1981

Immunological studies on chronic active hepatitis: possible involvement of macrophage-mediated cytotoxicity in its immunopathogenesis.

The possible involvement of cell-mediated immune responses to liver-specific protein in the pathogenesis of liver injury was investigated. The subjects consisted of seven patients with acute hepatitis, 12 cases with chronic active hepatitis, four cases with chronic inactive hepatitis, and three cases with liver cirrhosis. When peripheral blood lymphocytes from these patients were cultured in the presence of liver specific protein, and lymphocyte transformation was determined by measuring the uptake of [3H]thymidine into acid-insoluble materials, positive blastogenesis was seen in two cases with acute hepatitis and in six cases with chronic active hepatitis. The macrophage activating factor (MAF), a kind of lymphokine, was also detectable in the culture medium of activated lymphocytes from six patients who showed positive blastogenesis by estimating [3H]glucosamine incorporation into macrophages. Furthermore, the MAF-activated macrophages wer shown to be cytotoxic for the isolated liver cells causing marked inhibition of albumin synthesis. This macrophage-mediated cytotoxicity was detected in eight cases that showed positive lymphocyte transformation. These observations suggest that macrophage-mediated cytotoxicity plays a role in the pathogenesis of chronic active hepatitis.

Adult↗
PubMed1981

Possible involvement of macrophage-mediated hepatocytotoxicity in chronic active hepatitis: the induction of liver cell injury by culture supernatant of MAF-activated macrophages.

When peripheral blood lymphocytes from patients with various types of hepatitis were stimulated in vitro with liver specific protein, lymphocyte transformation and MIF production were demonstrated in many cases, especially in chronic active hepatitis. The culture supernatant of these activated lymphocytes was also shown to contain MAF, a kind of lymphokine, which activated the peritoneal macrophages of guinea pigs. When the culture fluid of MAF-activated macrophages was added to isolated liver cells, a significant inhibition of their albumin biosynthesis was detected. The active principle which caused the impairment of liver function was fractionated by gel filtration using a Sephadex G-75 column, and it was found that the active material was a protein-like substance with a molecular weight of about 10,000-40,000. The results suggest the possibility that the soluble substance released from the activated macrophage may be involved at least partially in the immunological pathogenesis of chronic active hepatitis among many other immunological processes.

Adult↗
PubMed1981

Studies on intrahepatic cholestasis in drug-induced allergic hepatitis: intrahepatic cholestasis induced in the rat by the culture supernatant of activated lymphocytes.

A marked reduction in bile flow and bile acid excretion was whenever peripheral lymphocytes from patients with drug-induced allergic intrahepatic cholestasis were stimulated with a specific drug in vitro in the presence of a soluble liver-specific antigen fraction, and their culture supernatant injected into the mesenteric vein of rats. A gel filtration study of the active fraction of the supernatant that caused a reduction in bile flow, suggested that the molecular size of this active principle is similar to that of the macrophage migration inhibitory factor (MIF). Histologically, dilated bile canaliculi with decreased microvilli were observed via electron microscopy in rat liver after injection of culture supernatant. No such changes were observed in rats after injection of the supernatant of a lymphocyte culture similarly prepared from normal individuals. These results strongly suggested that sensitized lymphocytes obtained from patients with drug-induced intrahepatic cholestasis produce a factor (of factors) causing cholestasis when stimulated with a specific drug in the presence of liver-specific antigen fractions.

Animals↗
PubMed1980

Immunological studies on drug-induced allergic hepatitis--hepatocellular injury by macrophage-mediated cytotoxicity.

When the peripheral blood lymphocytes from patients with drug-induced allergic hepatitis were stimulated with a specific drug in vitro in the presence of a liver cytosol fraction containing liver specific antigen, lymphocyte transformation was seen in eight out of 11 patients. The macrophage activating factor (MAF), a kind of lymphokines, was also detectable in the culture medium of activated lymphocytes from seven out of eight patients who showed positive blastogenesis evaluated the uptake of 3H-glucosamine into macrophages. MAF-activated macrophages exhibited a cytotoxic effect on separated liver cells resulting in a marked inhibition of albumin synthesis. This macrophage-mediated cytotoxicity was also observed in eight out of 11 patients who showed positive lymphocyte transformation. These observations suggest that macrophage-mediated cytotoxicity may play some role in the pathogenesis of drug-induced allergic hepatitis.

Adult↗
PubMed1980

Left ventricular performance and compliance following acute febrile mucocutaneous lymphnode syndrome.

Right and left heart catheterizations and biplane cineangiographies were performed on 115 post-MCLS cases to study the left ventricular performance and compliance, and the following conclusions were obtained: 1) The left ventricular performance of the post-MCLS cases with macroscopic cardiac lesions such as coronary aneurysms and stenosis declined compared with the others. 2) The left ventricular contractility of the post-MCLS cases without macroscopic cardiac lesions were within normal limits. 3) The diastolic compliance of the post-MCLS cases seemed to be decreased but the value could be regarded as normal for children.

Child↗