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Biomedical subjects

Y Minamishima

Publications and source records attributed to Y Minamishima.

At least 109 records · Page 6Linked to original sources
PubMed1982

Serodiagnosis of tsutsugamushi fever (scrub typhus) by the indirect immunoperoxidase technique.

The indirect immunoperoxidase technique was assessed for the serodiagnosis of tsutsugamushi fever (scrub typhus). The antigens were peritoneal smears prepared from mice infected intraperitoneally with the Karp, Kato, and Gilliam strains of Rickettsia tsutsugamushi. Treatment of the mice with cyclophosphamide apparently increased the number of the rickettsiae, and it minimized the exudate that interfered with the specific staining. The rickettsiae were seen as clusters in the juxtanuclear region of the mesothelial cells and also as free particles outside of the cells. By the indirect immunoperoxidase technique, the sera from all of the patients (49 samples from 30 patients) were positive for the R. tsutsugamushi antibody. The antibody titers (immunoglobulin G [IgG] and IgM) determined by the indirect immunoperoxidase technique correlated with those determined by the indirect immunofluorescence technique. Thus, the indirect immunoperoxidase technique was useful for quantifying both IgG and IgM antibodies to the rickettsia.

Antibodies, Bacterial↗
PubMed1981

Preparation of stable target cells for anti-herpes simplex virus cytotoxic T lymphocytes.

Using an avirulent strain of herpes simplex virus (HSV), SKa, and a methylcholanthrene induced sarcoma cell line, Meth A cells, we have developed a reliable target cell system for detection of cell-mediated cytotoxicity directed against HSV-infected cells. SKa-infection in Meth A produced no progeny virus but induced HSV-specific surface antigens as revealed by radioimmunoassay using 125I-labeled HSV antibody. Spontaneous release of 51Cr from the SKa-infected Meth A cells was no more than that from uninfected control cells but a strong spontaneous 51Cr release was produced in Meth A cells infected with KOS, a virulent strain which produced a progeny virus in Meth A and was lytic for the cells. When used as a target, SKa-infected Meth A cells could detect HSV-specific cytotoxicity by spleen and lymph node lymphocytes of mice immunized with SKa and KOS. This system also detected effector cytotoxic lymphocytes stimulated in vitro by mixed cultures of immune spleen cells and KOS-infected Meth A cells. Thus, the system should be valuable in studies of cell-mediated cytotoxicity directed against HSV-infected cells.

Animals↗
PubMed1978

Murine model for immunoprophylaxis of cytomegalovirus infection. I. Efficacy of immunization.

Murine cytomegalovirus was utilized as a model for human cytomegalovirus, which had no experimental animal, to study immunoprophylaxis of the cytomegalovirus infections. (1) Murine cytomegalovirus (MCMV) serially propagated in mouse embryonic fibroblasts had lost pathogenicity for weanling mice including neonatally thymectomized mice. (2) The cell culture-adapted MCMV was effective as a "live, attenuated virus vaccine" against challenge by virulent, mouse-passaged MCMV. (3) The immunization via intraperitoneal route protected mice from every parameter of MCMV infection. These included clinical signs, virus replication, histopathology and mortality. (4) The protective immunity was active against the virulent MCMV which was not neutralized by the rabbit anti-attenuated MCMV serum.

Animals↗
PubMed1977

Immunoprophylaxis of experimental cytomegalovirus infection.

Experimental MCMV infection simulates human disease. Since human CMV has no experimental animal, the murine model is useful to study mechanism of immunity to CMV and immunoprophylaxis of CMV infection. The live attenuated MCMV, obtained by serial passages in MEF, was effective in immunization of mice against fatal MCMV infection. Every parameter of the MCMV infection was nullified by the prior immunization. The protective immunity was cell-mediated and independent of antibody production. The sensitized T lymphocyte interfered with virus replication in vitro and in vivo. Efficacy and possible risk of a human CMV vaccine were discussed with the murine model.

Animals↗
PubMed1971

Neutralizing antibodies to cytomegaloviruses in normal simian and human sera.

Simian and human sera were examined for neutralizing antibodies to simian and human cytomegaloviruses (CMV). Neutralizing antibody to simian CMV was found in sera from 12 of 12 African green monkeys, 8 of 10 rhesus monkeys, and 7 of 7 baboons captured in the wild. The antibody did not cross-react with human CMV strain AD169 but cross-reacted with human strain C87, particularly in the presence of complement. Thirty-six baboons and 10 rhesus monkeys born and hand-reared in captivity remained free of neutralizing antibody both to simian and human CMV for as long as 4 years. Fifteen of 24 human sera (63%) revealed only species-specific neutralizing antibody.

Adult↗
PubMed1968

Physicochemical and biological properties of fibrous Pseudomonas bacteriophages.

Two fibrous Pseudomonas phages, Pf1 and Pf2, have been isolated and characterized. The phages were serologically related and were indistinguishable morphologically when viewed by electron microscopy. Both phages formed minute turbid plaques. A simple method was devised for easy differentiation of such plaques from the bacterial lawn. The infected bacteria continued to grow and to liberate the phages into the medium. The phages were collected from the turbid "lysate" by salting out and were purified by differential centrifugation and density gradient centrifugation. The purified phages were sensitive to ultrasonics and to a proteolytic enzyme, Nagarse. A rapid lysis mutant was obtained from the progeny of plaquepurified Pf2. Pf2 was spontaneously produced by strain P28 of P. aeruginosa. Neither acridine orange nor antiserum affected the phage-producing capacity of the bacteria.

Adsorption↗