Biomedical subjects

Y Maeda

Publications and source records attributed to Y Maeda.

At least 19 recordsLinked to original sources

PubMed2007

Ultrasonically driven continuous process for vegetable oil transesterification.

A bench scale continuous process for the manufacture of biodiesel from neat vegetable oils under high power low frequency ultrasonic irradiation was investigated. The experimental studies explored variations in alcohol-oil stoichiometry and type of oil. Important parameters such as residence time and reaction volume were considered. The highest conversion was achieved when short residence time was employed. The transesterification under ultrasonic irradiation is mainly influenced by the residence time in the reactor and alcohol-oil molar ratio.

Esterification↗

PubMed2006

Director-configurational transitions around microbubbles of hydrostatically regulated size in liquid crystals.

A high-pressure technique is introduced which allows a continuous variation of the inclusion size in liquid crystal colloids. We use a nematic liquid crystal host into which micrometer-sized gas bubbles are injected. By applying hydrostatic pressures, the diameter of these gas bubbles can be continuously decreased via compression and absorption of gas into the host liquid crystal, so that the director configurations around a single bubble can be investigated as a function of the bubble size. The theoretically predicted transition from a hyperbolic hedgehog to a Saturn-ring configuration, on reduction of the particle size below a certain threshold, is confirmed to occur at the radius of a few micrometers.

Journal Article↗

PubMed2006

Purification, characterization, and potential applications of formate oxidase from Debaryomyces vanrijiae MH201.

Formate oxidase was found in cell-free extracts of Debaryomyces vanrijiae MH201, a soil isolate. After purification by column chromatography, the preparation showed a protein band corresponding to a molecular mass (MM) of 64 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The MM, estimated by a gel filtration, was 99 kDa. The preparation showed two and three bands on isoelectric focusing under denaturing and native conditions, respectively. These results suggest that the preparation contained three isoforms, each of which might be composed of alphaalpha, alphabeta, and betabeta subunits with apparently similar MM. The preparation acted on formate with K (m) and V (max) values of 11.7 mM and 262 micromol min(-1) mg(-1), respectively, at pH 4.5 and 25 degrees C, but showed no evidence of activity on the other compounds tested. The optimum pH and temperature were pH 4.0 and 35 degrees C, respectively. The preparation showed activities of 85% of the initial activity after storage at pH 6.0 and 4 degrees C for 8 weeks. When 10 mM formaldehyde was reacted with 2.0 U ml(-1) of the enzyme preparation at pH 5.5 and room temperature in the presence of 2.0 U ml(-1) of a microbial aldehyde oxidase and 100 U ml(-1) of catalase for 180 min, neither of formate nor formaldehyde was detected, suggesting that the reaction involved the quantitative conversion of formaldehyde to carbon dioxide.

Aldehyde Oxidase↗

PubMed2006

Aspects of ultrasonically assisted transesterification of various vegetable oils with methanol.

The batch transesterification of vegetable oil with methanol, in the presence of potassium hydroxide as catalyst, by means of low frequency ultrasound (40 kHz) was studied with the aim of gaining more knowledge on intimate reaction mechanism. The concentration of fatty acid methyl esters, of mono-, di- and triglycerides of the actual reaction mixture were determined at short reaction time by HPLC. The effect of ultrasounds on the lipids transesterification correlated with triglyceride structures is discussed. It was found that under ultrasonic activation the rate-determining reaction switches from DG-->MG (classical mechanic agitation) to MG+ROH-->Gly+ME (ultrasonically driven transesterification).

Journal Article↗

PubMed2006

Spin correlations of strongly interacting massive fermion pairs as a test of Bell's inequality.

We report the results of the first-time test of the local hidden variable theories (Bell-Clauser-Horne-Shimony-Holt) involving strongly interacting pairs of massive spin 1/2 hadrons from the decay of short-lived (tau<10;-21sec) 2He spin-singlet state, populated in the nuclear reaction 2H+;1H-->;2He+n. The novel features of this experiment are (a) the use of an 'event-ready' [corrected] detector of nearly 100% efficiency to prepare an unbiased sample and (b) a focal-plane polarimeter of full 2pi sr acceptance with a random "post selection" of the reference axes. The spin-correlation function is deduced to be S[exp](pi/4)=2.83+/-0.24stat+/-0.07sys. This result is in agreement with nonlocal quantum mechanical prediction and it violates the Bell-CHSH inequality of |S|<or=2 at a confidence level of 99.3%.

Journal Article↗

PubMed2006

Precision measurement of the quasifree pn-->dvarphi reaction close to threshold.

The quasifree pn-->dvarphi reaction has been studied at the Cooler Synchrotron COSY-Jülich, using the internal proton beam incident on a deuterium cluster-jet target and detecting a fast deuteron in coincidence with the K+K- decay of the varphi meson. The energy dependence of the total and differential cross sections are extracted for excess energies up to 80 MeV by determining the Fermi momentum of the target neutron on an event-by-event basis. Though these cross sections are consistent with s-wave production, the kaon angular distributions show the presence of p waves at quite a low energy. Production on the neutron is found to be stronger than on the proton but not by as much as for the eta meson.

Journal Article↗

PubMed2006

Influences of palatal side design and finishing on the wearability and retention of mouthguards.

OBJECTIVES: To examine the influences of design and finishing on mouthguard wearability and retention. MATERIALS AND METHODS: 17 students at the Dental Technician Institute at Osaka University School of Dentistry, Osaka, Japan, participated in this study after providing informed consent. For each student, a single-layer custom-made mouthguard was fabricated from a 3.8-mm-thick ethylene-vinyl acetate sheet using a standardised procedure to obtain a precise fit. Each mouthguard was modified by changing the margin location and shape through five consecutive steps. At each step, questionnaires with a visual analogue scale regarding wearability (comfort, breathing, speaking, swallowing, lip closure, temporomandibular joint fatigue and swallowing) and retention were completed by subjects after wearing the mouthguard for 5 min. Statistical analyses were carried out among the steps using Wilcoxon's signed-rank test with a significance level of p<0.05. RESULTS: Significant improvements were found for comfort, breathing, speaking and swallowing by trimming the palatal margin to the cervical area, smooth finishing and occlusal adjustment of the mouthguard (p<0.01). No significant differences were found for retention throughout the procedure. CONCLUSIONS: Within the limitations of this experimental study, design and finishing at the palatal side appear to have significant influences on mouthguard wearability, but not retention.

Adult↗

PubMed2006

Population research of genetic polymorphism at amino acid position 631 in chicken Mx protein with differential antiviral activity.

A single amino acid substitution between Asn and Ser at position 631 in the chicken Mx protein has been reported to determine resistant and sensitive antiviral activity. In this study, we investigate whether various kinds of chicken breeds and jungle fowls carry the resistant or sensitive Mx allelic gene by using the mismatched PCR-restriction fragment length polymorphism (RFLP) technique. In total, 271 samples from 36 strains of 17 chicken breeds and from 3 kinds of jungle fowls were examined. The rates of the resistant Mx gene and sensitive gene were 59.2% and 40.8%, respectively. Only a Red jungle fowl captured in Laos carried the resistant Mx gene, and the other three Red jungle fowls from Indonesia and Gray and Green jungle fowls all had the sensitive Mx gene. These results were confirmed by the determination of amino acid sequences in the GTPase effector domain of jungle fowls.

Alleles↗

PubMed2006

Near-threshold production of phi mesons in pp collisions.

The pp-->ppphi reaction has been studied at the Cooler Synchrotron COSY-Jülich, using the internal beam and ANKE facility. Total cross sections have been determined at three excess energies epsilon near the production threshold. The differential cross section closest to threshold at epsilon=18.5 MeV exhibits a clear S wave dominance as well as a noticeable effect due to the proton-proton final-state interaction. Taken together with data for ppomega production, a significant enhancement of the phi/omega ratio of a factor 8 is found compared to predictions based on the Okubo-Zweig-Iizuka rule.

Journal Article↗

PubMed2006

Phase I/II study of docetaxel and S-1 in patients with advanced gastric cancer.

The aims of this phase I/II study of docetaxel and S-1 were to determine the dose-limiting toxicity (DLT), maximum-tolerated dose (MTD), and recommended dose (RD) in the phase I part and to explore the tumour response, survival and safety in the phase II part. Patients with histologically- or cytologically confirmed unresectable or recurrent gastric cancer were eligible. Treatment consisted of intravenous docetaxel on day 1 (starting dose 50 mg m(-2)) and oral S-1 at a fixed dose of 40 mg m(-2) twice daily on days 1-14, every 4 weeks up to six cycles. Nine patients took part in the phase I portion of the study. The MTD of docetaxel was determined to be 50 mg m(-2), with the DLTs of grade 3 infection associated with grade 3 neutropenia and grade 4 neutropenia during S-1 administration. The RD of docetaxel was 40 mg m(-2) in combination with S-1 40 mg m(-2) b.i.d. The efficacy and safety of this regimen was therefore assessed in 46 patients with at least one measurable lesion. The overall response rate and estimated median overall survival were 46% (95% CI, 31-61%) and 14.0 months (8.3-17.3 months), respectively. The most common grade 3/4 toxicity was neutropenia (67% of patients), which was predictable and manageable. This regimen showed promising activity with moderate toxicities in advanced gastric cancer.

Adenocarcinoma↗

PubMed2006

Effect of coal ash on sonochemical degradation of phenol in water.

The influences of coal ash on the degradation of phenol in water were investigated under the stirring or ultrasonic irradiation conditions. Phenol solution (10mg/L, 100mL) was sonicated at 200 kHz and 200 W with or without coal ash (53-106 microm in particle size and concentration of 0.0-1.5 wt%). It was found that the sonochemical degradation of phenol in the presence of coal ash was faster than that in the absence of coal ash, and the optimum amount of coal ash was a maximum at 0.4-0.6 wt%. It was confirmed that the phenol degradation did not occur by the addition of hydrogen peroxide and nitric acid under the stirring conditions. The sonochemical degradation with coal ash was depressed by the addition of tertiary butyl alcohol as a radical scavenger. These results indicated that the coal ash accelerated the phenol degradation due to the increase in the amount of hydroxyl radicals under the ultrasonic irradiation. Since the coal ash used had a porous and uneven surface, which was observed by SEM, it was assumed that the coal ash led to the increase in the nucleation site for cavitation bubble due to its surface roughness.

Journal Article↗

PubMed2006

Evidence for an excited hyperon state in pp --> pK+Y0*.

Indications for the production of a neutral excited hyperon in the reaction pp --> pK+Y0* are observed in an experiment performed with the ANKE spectrometer at COSY-Jülich at p(beam) = 3.65 GeV/c. Two final states were investigated simultaneously, viz. Y0* --> pi+X- and pi-X+, and consistent results were obtained in spite of the quite different experimental conditions. The parameters of the hyperon state are M(Y0*) = (1480 +/-15) MeV/c2 and gamma(Y0*) = (60 +/- 15) MeV/c2. The production cross section for Y0* decaying through these channels is of the order of few hundred nanobarns. Since the isospin of the Y0* has not been determined here, it could either be an observation of the sigma(1480), a one-star resonance of the Particle Data Group tables, or, alternatively, a lambda hyperon. Relativistic quark models for the baryon spectrum do not predict any excited hyperon in this mass range and so the Y0* may be of exotic nature.

Journal Article↗

PubMed2006

Assignment of 117 genes from HSA5 to the porcine IMpRH map and generation of a dense human-pig comparative map.

Twenty-two and eight significant quantitative trait loci for economically important traits have been located on porcine chromosomes (SSC) 2q and SSC16 respectively, both of which have been shown to correspond to human chromosome 5 (HSA5) by chromosome painting. To provide a comprehensive comparative map for efficient selection of candidate genes, we assigned 117 genes from HSA5 using a porcine radiation hybrid (IMpRH) panel. Sixty-six genes were assigned to SSC2 and 48 to SSC16. One gene was suggested to link to SSC2 markers and another to SSC6. One gene did not link to any gene, expressed sequence tag or marker in the map, including those in the present investigation. This study demonstrated the following: (1) SSC2q21-q28 corresponds to the region ranging from 74.0 to 148.2 Mb on HSA5q13-q32 and the region from 176.0 to 179.3 Mb on HSA5q35; (2) SSC16 corresponds to the region from 1.4 to 68.7 Mb on HSA5p-q13 and to the region from 150.4 to 169.1 Mb on HSA5q32-q35 and (3) the conserved synteny between HSA5 and SSC2q21-q28 is interrupted by at least two sites and the synteny between HSA5 and SSC16 is also interrupted by at least two sites.

Animals↗

PubMed2006

Genetic polymorphisms at FCER1B and PAI-1 and asthma susceptibility.

BACKGROUND: We previously detected a promoter polymorphism (- 109C/T) in the gene for the beta-chain of the high-affinity receptor for IgE (FCER1B), which was associated with total serum IgE levels but not with asthma in a Japanese population. A genetic interaction is biologically plausible between FcepsilonRI-beta and the plasminogen activator inhibitor 1 (PAI-1), which is highly expressed in mast cells in asthmatics and plays an essential role in airway remodelling. We hypothesized that FCER1B promoter polymorphisms, by modifying the intensity of mast cell activation signals, modulate the genetic effects of a functional 4G/5G polymorphism in the PAI-1 gene on asthma. OBJECTICIVE: To examine whether FCER1B promoter polymorphisms (- 109C/T and - 654C/T) influence the genetic effects of the functional polymorphism (4G/5G) at the PAI-1 promoter region on asthma susceptibility using a case-control analysis. METHODS: Subjects (374 asthmatic patients and 374 non-asthmatic controls) were divided into combined genotype groups based on the presence of FCER1B - 109TT and - 654CC genotypes and the PAI-1 4G allele. Logistic regression analysis was used to estimate adjusted odds ratios for asthma associated with the different genotype groups. RESULTS: Individuals homozygous for the FCER1B - 109T/ - 654C haplotype and the PAI - 1 5G allele had a reduced susceptibility to asthma; the odds ratio for the development of asthma was 0.20 (95% confidence interval, 0.084 - 0.46; P = 0.00015) for them, compared with individuals also homozygous for the - 109T/- 654C haplotype at FCER1B but carrying the 4G allele at PAI-1. The regression model also showed an interaction of the PAI-1 4G/5G genotype with the FCER1B-109C/T (P for interaction = 0.0017) or FCER1B-654C/T (P for interaction = 0.031) on asthma. CONCLUSION: The present findings suggest a synergistic interaction between FCER1B and PAI-1 genes in asthma susceptibility.

Adolescent↗

PubMed2006

Sequence variants of the secreted phosphoprotein 1 gene are associated with total serum immunoglobulin E levels in a Japanese population.

BACKGROUND: Secreted phosphoprotein 1 (SPP1) is a cytokine with pleiotrophic immunological activities, including activation of macrophage chemotaxis and T-helper type 1 (Th1) immune responses. SPP1 gene polymorphisms have been shown to be associated with several immune inflammatory diseases including multiple sclerosis (MS), which is characterized by fewer allergic symptoms and lower numbers of allergen sensitizations. OBJECTIVE: The present study examined whether SPP1 gene polymorphisms are associated with total serum IgE levels, atopy and asthma in a Japanese population. METHODS: This case-control association analysis examined 611 subjects, including 268 subjects with asthma. We genotyped three promoter and two exon polymorphisms at SPP1: -1687A/G; -381T/C; -94 deletion/G; 5891C/T; and 7052T/C. Results Association analyses of SPP1 polymorphisms showed that homozygosities for the 5891T allele (P=0.009) and 7052C allele (P=0.001) were significantly associated with increased levels of total IgE in non-asthmatic subjects. However, these variants were not associated with asthma and atopy. Interestingly, individuals carrying the 5891C allele, which is more prevalent in patients with MS in Japanese populations, displayed significantly lower levels of total serum IgE. Individuals homozygous for the 7052C allele, which is associated with development of systemic lupus erythematosus, displayed significantly higher total serum IgE levels. CONCLUSION: These findings suggest that genetic polymorphisms in SPP1 may play a role in controlling basal levels of total serum IgE, independent of atopy.

Adult↗

PubMed2006

In vitro differences of stress concentrations for internal and external hex implant-abutment connections: a short communication.

The aim of this study was to clarify the difference in the stress distribution patterns between implants with external-hex or internal-hex connection systems using in vitro models. Three 13 mm fixtures with external-hex and internal-hex connections were installed into an acrylic bone analogue. One piece abutments of 7 mm height was connected. Strain gauges were attached to the abutment surface, and the cervical and fixture tip areas of the bone analogue surface. Vertical and horizontal load applied was 30 N. Data were normalized for each model by obtaining values relative to the sum of the three values. Almost the same force distribution pattern was found under vertical load in both systems. Fixtures with external-hex showed an increase in strain at the cervical area under horizontal load, while in internal-hex fixtures the strain was at the fixture tip area. Within limitations of our model study, it was suggested that fixtures with internal-hex showed widely spread force distribution down to the fixture tip compared with external hex ones.

Dental Abutments↗

PubMed2006

Assignment of 204 genes localized on HSA17 to a porcine RH (IMpRH) map to generate a dense comparative map between pig and human/mouse.

Bi- and uni-directional chromosome painting (ZOO-FISH) and gene mapping have revealed correspondences between human chromosome (HSA) 17 and porcine chromosome (SSC) 12 harboring economically important quantitative trait loci. In the present study, we have assigned 204 genes localized on HSA17 to SSC12 to generate a comprehensive comparative map between HSA17 and SSC12. Two hundred fifty-five primer pairs were designed using porcine sequences orthologous with human genes. Of the 255 primer pairs, 208 (81.6%) were used to assign the corresponding genes to porcine chromosomes using the INRA-Minnesota 7000-rad porcine x Chinese hamster whole genome radiation hybrid (IMpRH) panel. Two hundred three genes were integrated into the SSC12 IMpRH linkage maps; and one gene, PPARBP, was found to link to THRA1 located in SSC12 but not incorporated into the linkage maps. Three genes (GIT1, SLC25A11, and HT008) were suggested to link to SSC12 markers, and the remaining gene (RPL26) did not link to any genes/expressed sequence tags/markers registered, including those in the present study. A comparison of the gene orders among SSC12, HSA17, and mouse chromosome 11 indicates that intra-chromosomal rearrangements occurred frequently in this ancestral mammalian chromosome during speciation.

Acetyltransferases↗

PubMed2006

[Open stent-grafting for the distal arch aneurysm using a new delivery sheath].

Surgical graft replacement of the distal aortic arch aneurysm has been known to have rather poor outcome. Open stent-grafting has been employed in many institutions, but several complications have been reported. Especially, embolism of the distal artery by debris of aneurysmal wall is a serious complication. Therefore we have developed and applied a new delivery sheath. The sheath is constructed with a ready-made delivery sheath and a part of a ready-made tracheostomy tube. That is called "balloon sheath". Five patients underwent distal arch stent-grafting using the device, all of whom had excellent result. The merits of the sheath are smooth delivery of stent-graft and less invasiveness for aortic wall. The new device is considered to be able to prevent aortic wall injury and peripheral embolic complication during insertion of stent-graft.

Aged↗