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Biomedical subjects

Y Lu

Publications and source records attributed to Y Lu.

At least 559 records · Page 31Linked to original sources
PubMed1998

[Clinicopathological and diagnostic study of amyloidosis of oral maxillofacial area].

OBJECTIVE: To study the clinicopathological features of amyloidosis studied in oral maxillofacial area and the diagnostic methods. METHODS: HE staining and staining of alkaline Congo red, Immunohistochemical staining, Sections examined by transmitted and polarized light microscopy, Transmitted electron microscopy. RESULTS: 14 cases of the disease were reported. Clinically, the patients usually had progressive enlargement and solidness of the tongue (macroglossia, 12 cases), hard nodules or cords of the cheek and lip mucosa (8 cases), single or multiple hard masses of submandibular and submental areas (6 cases), and ecchymosis or petechiae of the face, neck, chest or oral mucosa. Microscopically, the disease is characterized by the even pink amyloid protein deposit in the soft tissues and the wall of blood vessles, which shows positive staining of alkaline Congo red, Immunohistochemical staining can differentiate the primary amyloidosis from secondary one. Ultrastructurally, Amyloid protein appeares as felt-like finely filament. CONCLUSION: Amyloidosis involving in oral maxillofacial area mainly arise in tongue, less frequently, in buccal and lip mucosa, salivary glands. It is not difficulty to dignosis the disease by HE staining combining with staining of alkaline Congo red and other specific staining methods. Ultrastructural and immunohistochemical study is helpful to make show the dignosis.

Adult↗
PubMed1998

[The treatment of rat fulminant hepatic failure by auxiliary partial heterotopic liver transplantation: experimental study].

OBJECTIVE: To confirm the effect of auxiliary partial heterotopic liver transplantation (APHLT) in the treatment of fulminant hepatic failure (FHF). METHOD: On 30 rat models of FHF, 62% of partial liver grafts were implanted below the host residual liver with 28 successful operations. The survival rate, biochemical test for liver function, (99m)Tc-HIDA liver scintigraphy and pathological changes of the grafts and host livers were studied. RESULT: The survival rate in 48 hours was 71.4% compared with 0% in 15 FHF animals. 14 days after the operation, the host liver regenerated obviously and the liver function recovered while the graft shrank gradually. 30 days later, the graft became fibrosis completely. CONCLUSION: Depending on the temporary function support of the graft, the native residual liver could regenerate and restore its function while the graft tended to atrophy.

Animals↗
PubMed1998

[The effects of epinephrine and adrenergic antagonists on adenosine 3', 5'-monophosphate level of bovine trabecular cells in vitro].

OBJECTIVE: To determine the effects of epinephrine (EPI) and adrenergic antagonists on adenosine 3', 5'-monophosphate (cAMP) level of bovine trabecular cells (BTC) in vitro. METHOD: (3)H-cAMP was used in protein binding assay for measuring the intracellular level of cAMP. RESULTS: (1) 10(-5) mol/L EPI induced a fold increase of cAMP in cultured BTC in vitro; (2) Timilol and ICI 118, 551 blocked efficiently the effect of EPI at a lower concentration (10(-6) mol/L). (3) Bisoprolol did not efficiently block the effect of EPI unless at high concentrations (>or= 10(-5) mol/L). CONCLUSION: The effects of EPI increasing outflow facility may be associated with its increase of cAMP in trabecular cells; BTC contains beta-adrenergic receptors, and beta(2)-adrenergic receptors are dominant.

Adrenergic beta-Antagonists↗
PubMed1998

[The regulating effects of dopamine receptors on the level of adenosine 3, 5-cyclic monophosphate in bovine trabecular cells in vitro].

OBJECTIVE: To assay the regulating effects of dopaminergic agonists and antagonists on adenosine 3, 5-cyclic monophosphate (cAMP) level in bovine trabecular cells (BTC) in vitro. METHOD: H(3)-cAMP was used in protein binding assay to determine the level of cAMP in bovine BTC. RESULTS: Dopamine, a dopamine receptor agonist, and fenoldopam, a selective DA(1) receptor agonist, caused a fold increase of cAMP in cultured BTC in vitro. These effects were blocked by halporide, a non-selective dopamine antagonist, and SCH23309, a selective DA(1) antagonist, but not blocked by phenoxybenzamin, an alpha-adrenergic antagonist, and timolol, a beta-adrenergic antagonist. CONCLUSION: Bovine trabecular cells contain not only beta-receptors but also DA(1) receptors.

Animals↗
PubMed1998

[RP-HPLC method for determination of isorhynchophylline in plasma and its pharmacokinetics in rabbits].

A reversed phase HPLC method for determination of isorhnchophylline (IRHY) in rabbit plasma was developed. A Shimadzu LC-10A liquid chromatograph equipped with a Shimadzu SPD-10A multiwavelength detector and a Chromatopac LC-10A data processor were used. The analytical column was a 4.6 mm x 150 mm CLC-ODS. The mobile phase was a mixture of methanol-water (95:5), the flow rate was 1 ml.min-1. Detection was performed at UV 254 nm. The calibration curve was linear in the range from 0.016 to 16 micrograms.ml-1 with gamma = 0.9990. The lowest detection limit was 0.016 microgram.ml-1. The precision and accuracy of within-day and day-to-day ranged from 3.8% to 6.9%. The recoveries were 80.5% to 85.1%. The drug concentrtion-time curves of IRHY in rabbits after i.v. of 2 mg.kg-1 and 5 mg.kg-1 were shown to fit a two-compartment open model with half-times of 1.32 +/- 0.6 h and 1.25 +/- 0.09 h respectively. The absorption was fast after intraduodenal administration of IRHY (2 mg.kg-1 and 5 mg.kg-1) to rabbits with half-times of 1.75 +/- 0.18 h and 1.26 +/- 0.20 h, respectively. The bioavailability of IRHY was about 42.4% to 69.4%. The results showed that the method was simple and fast. The absorption and elimination were fast atfer intraduodenal administration or i.v. of IRHY to rabbits.

Alkaloids↗
PubMed1998

[The development of a central monitor system for fetus].

A central monitor system for fetus is described. It is a network composed of a central machine and several fetal monitors. The monitors are connected to the central machine by special lines within hospital and by phone line outside hospital.

Cardiotocography↗
PubMed1998

[Study on voltammetric behaviour of clozapine and its single-sweep oscillopolarography].

A sensitive reduction peak of clozapine is obtained by single-sweep oscillopolarography in 0.1 mol.L-1 H2SO4 solution. The peak potential is -0.95 V (vs. SCE). The peak current is directly proportional to the concentration of clozapine with a detection limit of 2.0 x 10(-8) mol.L-1. The voltammetric behaviour of clozapine and its mechanism of electrode reaction have been studied by linear-sweep and cyclic voltammetry and pulse polarography etc. The reduction process is irreversible with adsorptive characteristics. The number of electrons transferred n is 2. The electron transfer coefficient alpha is 0.62. Its behaviour obeys Langmuir adsorption isotherm. The adsorption coefficient beta is 1.91 x 10(6) L.mol-1. The energy of adsorption delta G0 = -35.83 kJ.mol-1. The method has been applied to the determination of clozepine in tablet and urine.

Antipsychotic Agents↗
PubMed1998

[X-ray diffraction Fourier Pattern Analysis of the Chinese materia medica "shannai" (Kaempferia Galanga L.)].

Every Chinese materia medica consists of dozens of ingredients. It is a multiingredient system. X-ray diffraction provides a way to determine Chinese materia medica in diffraction space which can reflect the general structural characteristics of Chinese materia medica and quantitative changes of partial ingredients. Considering the influence of varieties, growing places, growing periods, collecting seasons and other phenological factors, we selected 6 samples of Kaempferia galanga L. and Kaempferia marginata Carey, collected in different seasons from different growing places for X-ray diffraction Fourier Pattern Analysis. We have obtained characteristic peaks and diffraction fuzzy figures for distinguishing purposes. The result shows that X-ray diffraction Fourier Pattern Analysis can be used for distinguishing Chinese materia medica with similar shape and properties, similar tissue texture or similar chemical composition.

Drug Contamination↗
PubMed1998

[Studies on the chemical constituents of chloroform extract of Dracaena cochinchinensis].

Six compounds were isolated from Dracaena cochinchinensis (Lour.) S.C. Chen. Their structures were identified as 2,3,5,6-tetracchloro-1,4-dimethoxybenzene (I), 7-hydroxy-4'-methoxyflavan (II), 4'-hydroxy-3,5-dimethoxystilbene (III), 4'-hydroxy-2,6-dimethoxydihydrochalcone (IV), 4'-hydroxy-2,4,6-trimethoxydihydrochalcone (V) and 6-hydroxy-7-methoxy-3-(4'-hydroxybenzyl) chromane (VI) by chemical method and spectral analysis. IV and VI are new compounds.

Chalcone↗
PubMed1998

[Studies on the fine stereostructure of taxoids].

The anticancer drug taxol is a new type antimicrotubular drug. The structure-activity relationship study on taxol and its analogs has indicated the importance of the C13 side chain and the C4-C5-C20 epoxypropane group. The crystal structures of 4 taxoids having the basic skeleton with 5/7/6 membered ring and three types of 10 taxoids having the basic skeleton with 6/8/6 membered ring have been analyzed. The effects of the induction by different substructures at C4 on the stereostructures of taxoids were studied, and the relationship between the 6/8/6/4 skeleton and the anticancer activity was discussed from the crystallographic point.

Antineoplastic Agents, Phytogenic↗
PubMed1998

[The relationship between protein synthesis of Sindbis virus and host cytoskeletons].

In our experiments, protein synthesis of host cells were inhibited quickly at the early stage of infection by Sindbis virus. Polysome and mRNA of host cell fell off from cytoskeletons, whereas virus RNA bound up. We also found it was via 3'-terminal that virus RNA bound with cytoskeleton. After studying on the virus nonstructural proteins, we found the synthesis and processing of virus protein in vitro were far slowly than in vivo, and most of proteins were premature. So, the cytoskeletons may play an important role there. After treated with colchicine and cytochalasin B, the microtubule and microfilament were destroyed. However, the synthesis and processing of nonstructural proteins of Sindbis virus didn't change much, while the structural proteins were inhibited largely. These results showed the differences of dependence of the synthesis of the two kinds of proteins on cytoskeletons. Microtubule and microfilament may be more important to the synthesis of structural proteins than to that of the nonstructural proteins.

Actins↗
PubMed1998

[The development of medical infusion monitoring and controlling network system].

The paper provided a medical infusion monitoring and controlling network system, in which the mainframe and its extensions can realize communication with each other by means of asynchronous serial port. At the same time, every extension can test infusion speed and adjust it to the presetting infusion speed gradually. The extensions also can alarm and stop infusion automatically when infusion will be over or there is a infusion malfunction.

Computer Communication Networks↗
PubMed1998

[Expression of P27 protein in odontogenic tumors].

The expression of P27 protein was investigated in 40 cases of odontogenic tumors by immunohistochemical S-P assay. The results showed that P27 expression varied in different types of odontogenic tumors. Positive signals were present in 17/20 (85.0%) of the malignant odontogenic tumors, but in 6/20 (30.3%) of the benign ones (P < 0.005). It was suggested that P27 protein had certain practical value in the differentiation diagnosis of odontogenic tumors.

Ameloblastoma↗
PubMed1998

[A stereological study of the remaining parotid gland of rat after duct-preserved partial parotidectomy].

The remaining parotid glands of rats after duct-preserved partial parotidectomy were sectioned and examined randomly by stereological methods which determine acinararea and proportional volumes (PV) of the component tissues. The volume of the remaining glands were measured at the same time. Results showed that: 1. there were no difference of the above quota 1-3 days after the operation (P > 0.05); 2. 1-2 weeks after the operation, the gland volume decreased (P < 0.05) with the PV of acini decreasing (P < 0.05); 3. 4-7 weeks after the operation, the acinar area increased (P < 0.05), the PV of acini and gland volume regained (P > 0.05). All the results indicate, with the duct preserved, the remaining parotid glands after partial parotidectomy do have the regenerating ability and significant function.

Animals↗
PubMed1998

[Detection of proviral gene of human immunodeficiency virus type 1 from dried blood spots].

A method for detection of proviral human immunodeficiency virus type 1 DNA fragment encoded pol gene in dried blood spots on filter paper by nested primers polymerase chain reaction (nPCR) has been developed. The filter paper was pre-saturated and dried by EDTA proteineas K. About 50 microliters whole blood from HIV1 infected individuals was collected and onto the piece of filter paper, and the paper was air dried stored sealed in a plastic bag at 4 degrees C or room temperature for 1 to 64 weeks. The PCR with outer primers was performed in 0.5% ml tube with the piece of filter paper. Following the PCR with the inner primers was finished. The results showed that template DNA could detected in piece of filter papers which stored at 4 degrees C for 40 weeks as well as stored at room temperature for 24 weeks. The results could directly determined by ethidium bromide staining of the PCR products when it compared with the PCR products of standard positive control and DNA molecular weight marker in electrophoresis analysis on agarose gel. The method is rapid, specific, and sensitive and has the ability to detect as little as 10 proviral template DNA. The collected samples could mail from basic laboratory to center laboratory and the method could be applied in confirming and screening of HIV1 infection.

Blood Stains↗
PubMed1998

[Effects of different amount of ginger juice on prepared products of Rhizoma Pinelliae].

Rhizoma Pinelliae were diged in Ginger juice of different propertion, the total alkaloid contents of these products were all higher than the raw Rhizoma Pinelliae, and related to the actions of anti-tumous cell. The contents of beta-sitosterol of these products were related to the amount of Ginger juice which is added in Pinelliae Rhizoma, but only the content of beta-sitosterol of the Pinelliae product which is prepared by same amount of Ginger juice was slight higher than the raw Rhizoma Pinelliae. The stimulations of five kinds of products on mice have no obvious relation to the amounts of Ginger juice which were added in Rhizoma Pinelliae, but the stimulation of the Pinellia product which was prepared by same amount of Ginger juice was the lowest of all products on mice. Dependent on above experiment results, we suggest that Rhizoma Pinelliae processed by Ginger juice should be prepared by same amount of Ginger juice with Rhizoma Pinelliae.

Alkaloids↗