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Biomedical subjects

Y Kondo

Publications and source records attributed to Y Kondo.

At least 973 records · Page 54Linked to original sources
PubMed1977

A rapid fluorometric assay of angiotensin-converting enzyme.

A rapid, simple and sensitive method is described for the fluorometric assay of angiotesin-converting enzyme using Fluorescamine. The critical factors such as optimal pH, incubation time, chloride ion, and inactivation by EDTA and 8-hydroxyquinoline were examined. The Km value for hippuryl-L-histidyl-L-leucine was 0.5 mM. This method was applied to the assay of angiotensin-converting enzyme in the rat serum and the reproducible values were obtained with a 10 mul of the rat serum.

Animals↗
PubMed1977

Demonstration of granulopoietic factor(s) in the plasma of nude mice transplanted with a human lung cancer and in the tumor tissue.

A human lung cancer (OTUK-tumor) was transplanted serially to nude mice, which invariably developed a marked neutrophilia. In order to analyze this phenomenon, in vitro agar culture studies were carried out. A three- to fourfold increase of colony-forming units in culture was observed in the femurs of these nude mice. Moreover, the plasma of nude mice bearing this tumor, as well as the tumor extract, had a significantly higher colony-stimulating activity on mouse bone marrow cells than appropriate controls. This activity had the property to stimulate granulocyte and/or mixed cell type colonies rather than mononuclear cells. This activity was also demonstrated, in a dose-dependent way, on normal human bone marrow cells. These findings indicated that the OTUK-tumor produced human granulopoietic colony-stimulating activity, which may have stimulated granulopoiesis in vivo as well.

Animals↗
PubMed1977

Frozen preservation of human malignant tumors transplanted into nude mice.

Five transplantable tumors, which originated from human maligant neoplasms and had been maintained in nude mice, were frozen at--70 degrees preserved for 1226 days. Tumor tissue was minced in a mixture of tissue culture medium, 10% dimethyl sulfoxide, and 10% calf serum before freezing. The transplantability of frozen tumors was examined after thawing. Morphological and functional characteristic of each tumor were compared before and after the frozen storage. The results suggest that low-temperature preservation technique can be applied satisfactorily for the maintenance of human malignant tumors which have been maintained by using nude mice.

Animals↗
PubMed1976

Retrograde axoplasmic transport to inactive dopamine beta-hydroxylase in sciatic nerves.

The transport of dopamine-beta-hydroxylase (DBH) in sciatic nerves was studied both by measuring the accumulated enzyme activity and by examining the accumulated immunologically reactive DBH protein. The pattern of DBH accumulation in the proximal segment was similar either in the activity or in the immunofluorescence. In the distal segment, however, much higher accumulation of immunofluorescence than that of the activity was observed 7 h after ligation. The immunotitration studies indicated that more DBH molecules were accumulated in the distal segment than in the proximal segment 5 h after ligation. The homospecific activity of DBH in the distal segment was about one-fifth of that in the proximal segment. The result suggests that relatively large amounts of inactive enzyme may be transported back to the soma instead of complete degradation in the terminal.

Animals↗
PubMed1976

Purification and some properties of microsome-bound thyroglobulins.

The sonicate of microsomal fraction of hog thyroid was found to contain two species of proteins which reacted with anti-thyroglobulin antiserum and corresponded to 2 to 3% of total protein of the fraction. The two species were purified by ammonium sulfate precipitation, repeated sucrose density gradient centrifugations and separated from each other by polyacrylamide gel electrophoresis into S-mb thyroglobulin (the slower-migrating microsomal-bound fraction), F-mb thyroglobulin (the faster-migrating microsomal-bound fraction), and thyroglobulin itself. Amino acid compositions of the proteins were essentially the same as that of thyroglobulin. Mannose contents also were close to that of thyroglobulin while galactose and fucose contents decreased in the order: thyroglobulin, F-mb thyroglobulin and S-mb thyroglobulin. S-mb thyroglobulin had no sialic acid and no iodine at all. F-mb thyroglobulin was sialylated to an extent of less than 80% of, and iodinated to an extent of less than 50% of that of thyroglobulin, respectively. Antigenic activities of F-mb thyroglobulin and of S-mb thyroglobulin were qualitatively the same as, but quantitatively less than, that of thyroglobulin. Sedimentation coefficients of S-mb thyroglobulin and F-mb thyroglobulin were approximately 1 S lower than that of thyroglobulin. All the properties of the thyroglobulin-related proteins suggested that S-mb thyroglobulin and F-mb thyroglobulin were the products by the sequential modification of thyroglobulin polypeptide in the pathway of thyroid hormone production.

Amino Acids↗
PubMed1976

Ascites form of a human cancer serially transplantable in nude mice.

Human adenocarcinoma cells injected into the peritoneal cavities of BALB/c nude mice (nu/nu) induced ascites carcinoma. The inoculant was obtained from subcutaneous tumors produced in nude mice by an injection of ascites cells from a patient with carcinomatous peritonitis caused by mucinous adenocarcinoma of the stomach. An ascitic fluid began to accumulate 45 days after inoculation and reached the maximum volume within 120 days. Dispersed stomach cancer cells in the ascites could be serially transplanted in nude mice in an ascites form. The morphology of these cells was similar to that of the original cells in the ascitic fluid of a patient with carcinomatous peritonitis.

Adenocarcinoma, Mucinous↗
PubMed1976

[Effect of selective proximal and truncal vagotomy on canine gastric electrical activity (author's transl)].

Alterations in the electrical activity of the stomach were studied in 40 unanesthetized dogs with intact vagal innervation, with selective proximal vagotomy (SPV) and with truncal vagotomy (TV) using a chronically implanted unipolar needle electrodes. The basic electrical rhythm (BER) of the normal canine stomach occurred regularly in the frequency of 4.5 to 5.5 cycles per minutes with a waxing and waning of initial and second potentials repeated in every 5 to 6 minutes. After SPV, irregular BER was observed occasionally within a few days postoperatively. After TV, irregularity of BER was more marked and frequent for the first 2 weeks. Subcutaneous administration of bethanechol chloride or histamine decreased the frequency of regular BER and increased the magnitude of second potentials equally in any dog of the control, SPV and TV groups. Similar changes were observed after feeding of the canned meat. Insulin-induced hypoglycemia increased the magnitude of antral second potentials in either control or SPV dogs. In contrast, TV dogs revealed repetition of initial potentials shortly after intravenous adminstration of insulin, but failed to show any changes relevant to hypoglycemia.

Animals↗
PubMed1976

Cellular aspects of rabbit Masugi nephritis. III. Mesangial changes.

Lytic disarrangement of mesangial matrix was noted in acute proliferative glomerulonephritis induced in the rabbit by injection of duck nephrotoxic antibody. The severity of the change was variable depending upon dose of the antibody injected. With severe mesangiolysis, mesangial areas were highly obscured by marked hypertrophy and proliferation of mesangial cells along with massive infiltration of monocytes into the loosened mesangium and subendothelial space. In addition, there was random mobilization of the mesangial cells as shown by peripheral interposition and intraluminal projection with capillary subdivision. Glomerular capillaries were thus narrowed and distorted. The ultrastructural hallmark of the proliferated mesangial cells was prominence of the Golgi complex and endoplasmic reticulum which suggested accelerated protein synthesis, most likely for repair of the damaged matrix. In the affected glomeruli, exclusive of those undergoing rapid obliteration due to basement membrane rupture, resolution of the acute inflammatory process occurred within 2 weeks. At this stage, two types of glomerular changes became manifest, consisting of: (1) prominent mesangial stalk thickening and cellularity with occasional peripheral interposition of mesangial cells, and (2) segmental capillary subdivisions due to mesangial bridges built between the original mesangium and peripheral loop. It is suggested that chronic glomerular disease may develop based upon these persistent alterations of the glomerular structure. When a smaller dose of the duck antibody was employed, the process was much less prominent and complete recovery from acute glomerulonephritis took place.

Animals↗
PubMed1976

Oral glucose tolerance test in the cynomolgus monkey (Macaca fascicularis).

The glucose tolerance test was simplified so that it could be performed on more than one cynomolgus monkey (Macaca fascicularis) at a time. Glucose was administered orally in a dose of 2 g/kg body weight as a 50% aqueous solution, and blood samples were taken just before and 30 minutes and 150 minutes after glucose administration. The simplified test was conducted on a total of 93 cynomolgus monkeys. Glucose tolerance curves obtained by this method were classified into three patterns with regard to the increasing rate of serum glucose concentration per minute and the decreasing rate. Seventy-one monkeys showed the first pattern characterized by an increasing rate of 1.0 mg/min or more and a decreasing rate of 0.2 mg/min or more. The second pattern showing an increasing rate of 1.0 mg/min or more and a decreasing rate of less than 0.2 mg/min was obtained with 10 monkeys. The remaining 12 monkeys exhibited the third pattern in which the increasing rate and the decreasing rate were less than 1.0 mg/min and 0.2 mg/min, respectively. The first pattern is considered to be normal glucose tolerance, while the second and third patterns are regarded as abnormal ones. The incidence of the abnormal patterns was significantly higher in wild-imported cynomolgus monkeys than in laboratory bred monkeys.

Animals↗