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Biomedical subjects

Y Jin

Publications and source records attributed to Y Jin.

At least 181 records · Page 10Linked to original sources
PubMed2000

Effects of hepatic artery chemotherapeutic embolization combined with perfusing LAK cells into hepatic artery after radical operation of liver cancer.

OBJECTIVE: To evaluate the effects of hepatic artery chemotherapeutic embolization combined with perfusing LAK cells/interleukin 2 into hepatic artery after radical operation of liver cancer. METHODS: Random divide 42 cases into two groups who had accepted radical operation of liver cancer: 21 cases who accepted hepatic artery chemotherapeutic embolization combined with perfusing LAK cells/interleukin 2 into hepatic artery as observing group, 21 cases accepted simply hepatic artery chemotherapeutic embolization as control group. RESULTS: The intrahepatic recurrence rates of 1, 2, and 3 years were 0, 19.05%, 57.14% in observing group, lower than 28.57%, 47.62%, 85.71% of control group (chi(2)=4.86, 3. 86 and 4.20 respectively, P<0.05). The survival rates of 2 and 3 years were 85.71%, 61.90% in observing group, higher than 57.14%, 28. 57% of control group (chi(2)=4.20 and 4.71, P<0.05). CONCLUSION: Hepatic artery chemotherapeutic embolization combined with perfusing LAK cells/interleukin 2 into hepatic artery is an effective therapeutic method to control intrahepatic recurrence rates and raise the survival rates of the patients.

Adult↗
PubMed2000

[Chromosome localization of the dentinogenesis imperfecta type II locus].

OBJECTIVE: To scrutinize the linkage between dentinogenesis imperfecta type II and chromosome 4q21 in a Tianjin-Tanggu family. METHODS: Blood samples were collected from 13 members of the family. DNA was analyzed with 4 short tandem repeat polymorphisms markers UGATA62A11, DSP(P), SPP1 and D4S1563 Y using fluorescence-based PCR. The linkage between four markers on chromosome 4q21 and dentinogenesis imperfecta type II was tested by Lod score analysis. RESULTS: GATA62A11 and DSP(P) suggested linkage and yielded a Lod score of 1.63 at theta =0, and 1.68 at theta =0 by means of the MLINK software, respectively. Genotype and haplotype were acquired. CONCLUSION: The disease gene of the dentinogenesis imperfecta type II family is located on chromosome 4q. The result will be helpful for the further identification of the dentinogenesis imperfecta type II gene.

Chromosome Mapping↗
PubMed2000

[Preparation and identification of hammerhead ribozyme in vitro against rat caspase-3 mRNA fragment].

OBJECTIVE: To study the transcript effect and cleavage activity in vitro of rat Caspase-3 specific ribozyme (Rz107 and Rz544). METHODS: Rat caspase-3 gene fragment was cloned into T-vector under the control of T7 promoter. (32)P-labeled caspase-3 transcript was target-RNA. Rz107 and Rz544 genes against caspase-3 mRNA were cloned and transcribed in vitro. Cleavage reaction was detected. RESULTS: It was found that Rz107 was active at 37 degrees C and more so at higher temperature within allowing temperature range. The optimal temperature was 50 degrees C. For Rz107, Km and kcat was 14.13 nmol/L and 2.31 min(-1), respectively. However, the Rz544 had no cleavage activity at all. CONCLUSION: Rz107 prepared in vitro possesses the perfect specific catalytic cleavage activity. It is hopeful that Rz107 would be developed to be a new nucleic acid drug that could effectively inhibit the inflammation of hepatitis through cleaving the key gene, caspase-3, in apoptosis in vivo.

Animals↗
PubMed2000

[Continuous monitoring of hepatic venous oxygen saturation (SHVO2) as a new diagnostic indicator of abdominal malperfusion in acute aortic dissection].

A 73-year-old woman was admitted with sudden-onset back and abdominal pain. Computed tomography scanning revealed type B acute aortic dissection with narrowing of the true lumen. We inserted an oximetric catheter into the right hepatic vein and started continuous measurement of ShvO2. The initial value was 20%. Consecutive aortograms showed an intimal tear in the thoracic descending aorta. Endovascular stent graft placement was performed to close the entry, and ShvO2 rose to more than 60% immediately after the stent graft expansion. ShvO2 is an excellent indicator of abdominal blood flow, not only for early diagnosis but also for the evaluation of treatment.

Abdomen↗
PubMed2000

[The effect of estrogen on new bone formation in the implanted bone matrix in aged male rats].

OBJECTIVE: To study the effect of estrogen on new bone formation in the implanted bone matrix in aged male rats and on general bone metabolism. METHODS: The aged male rats were implanted with slices of bone matrix and subcutaneously injected with estrogen, androgen or the solvent for five weeks. The changes in bone histomorphometry, bio-mechanical parameters and urine deoxypyridinoline (Dpd) levels were observed as indexes of bone reabsorption. Calcium, organic phosphate, and alkalphophatase levels in serum and urine were also studied. RESULTS: (1) The areas of new bone formation were significantly larger in estrogen and androgen groups than in the control but without difference between estrogen and androgen groups. (2) The number of osteoclasts was higher in estrogen and androgen groups than in the control (P < or = 0.002) but without difference between estrogen and androgen groups. (3) Urine Dpd/Cr ratio was lower in estrogen and androgen groups than in the control and higher in the control than that obtained 5 weeks ago but without difference between estrogen and androgen groups. CONCLUSION: Estrogen and androgen are able to stimulate new bone formation in the implanted bone matrix and inhibit the reabsorption rate of systemic bones in aged male rats, but there was no difference between estrogen and androgen in these two respects under the conditions of this experiment.

Androgens↗
PubMed2000

[The effects of beta-aminopropionitrile on the proliferation in cultrured human hypertrophic scar fibroblasts].

OBJECTIVE: To observe the effects of BAPN on the proliferation in cultured human hypertrophic scar fibroblasts. METHODS: By means of colorimetric MTT(tetrazolium) assay and flow cytometry, we tried to find the effects of BAPN with different concentrations and different time on the proliferation in cultured human hypertrophic scar fibroblasts. RESULTS: Concentrations of BAPN 0.074, 0.222, 0.667, 2.0, 6.0 mg/ml could inhibit proliferation in cultured human hypertrophic scar fibroblasts, but had no effects on cultured human normal dermal fibroblasts. Its ID50 is 2.53 mg/ml. CONCLUSIONS: BAPN could inhibit proliferation in cultured human hypertrophic scar fibroblasts and was dependent on concentration and time.

Adolescent↗
PubMed2000

[Individual biomass of natural Pinus densiflora].

The aboveground biomass of individuals with different growth potentials in natural Pinus densiflora forest with different stand densities was measured in Yanbian, Jilin Province. The variation of individual biomass affected by densities was in order of dominant tree < intermediate tree < suppressed tree, while the distribution proportion of biomass in different organs affected by densities was: in order of trunk > branch > needle > bark. The biomass components of P. densifliora with different growth potentials varied markedly with the approaching of density class III, and the change of intermediate trees was similar to the whole stand. The vertical distributions of biomass of different trees were different from each other, but all showed that the biomass of trunks and barks was mainly distributed below 6 m high from ground, that of branches was within 6-10 m high, that of needles was uniform in the upper, middle and lower layers, and that of branches and needles in upper layer was least affected by density.

Biomass↗
PubMed2000

[Effect of far-ultraviolet radiation on lipid peroxidation and inherent protection system in seedlings of Taxus cuspidata].

The effect of far-ultraviolet radiation on lipid peroxidation and inherent protection system in seedlings of Taxus cuspidata was examined with simulated UV-BC irradiation treatments. The electrolyte leakage, lipid peroxidation, tissue autoxidation and H2O2 content increased, while the contents of soluble protein, ascorbic acid, carotenoids, and chlorophyll and the photosynthetic electron transport rate of Photosystem II decreased with the increasing time of treatment. The lipid peroxidation of the needles was inhibited by exogenous scavengers sodium benzoate and ascorbate, but enhanced to some extent by methyl viologen and DDC (an inhibitor of SOD). The mechanisms of the injury of the seedlings induced by UV-BC radiation might be similar to that of photooxidation injuries of conifers in winter. However, Taxus cuspidata was much more resistant to UV-BC radiation than crops.

Antioxidants↗
PubMed2000

[Detection of DNA extracted from Mycobacterium tuberculosis by TaqMan-PCR technique and its clinical application].

OBJECTIVE: To evaluate the clinical diagnostic value of TaqMan-PCR technique in the diagnosis of active pulmonary tuberculosis. METHODS: The specimens of sputum and peripheral blood from 168 patients with active pulmonary tuberculosis, 57 patients with lung cancer and the specimens of peripheral blood from 34 healthy individuals were detected by TaqMan-PCR, PCR. The specimens of sputum were also detected by smear, culture with BACTEC and Lowenstein-Jensen for comparison. RESULTS: The positive rates of sputum and peripheral blood detected by TaqMan-PCR were 53.0% and 61.3%, respectively, significantly higher than those of PCR, smear, culture with BACTEC and Lowenstein-Jensen (P < 0.05). There was no significant difference between TaqMan-PCR and PCR for specificity (P > 0.05). Of 105 specimens of peripheral blood from patients with smear-negative and culture-negative pulmonary tuberculosis, 48 specimens of peripheral blood detected by TaqMan-PCR were positive. The positive rate was 45.7%. CONCLUSIONS: TaqMan-PCR method showed higher sensitivity and specificity. It is an useful tool for diagnosis of pulmonary tuberculosis, particularly smear-negative and culture-negative pulmonary tuberculosis.

DNA, Bacterial↗
PubMed2000

[Pulmonary embolism due to deep venous thrombosis of extremities].

OBJECTIVE: To study the relationship between pulmonary embolism and deep venous thrombosis of extremities. METHODS: The authors conducted a retrospective analysis of 100 patients with deep venous thrombosis to analyse the occurrence of pulmonary embolism during 1996 to and 1998 in their hospital. RESULTS: In the 100 patients with deep venous thrombosis, the complication of pulmonary embolism was 45%, fatal pulmonary embolism was 4%. CONCLUSION: The complication of pulmonary embolism in the patients with deep venous thrombosis frequently occurred and medical professionals should pay attention to them.

Adult↗
PubMed2000

[The role of bcl-2 and bax genes and programmed cell death in the temporomandibular joint development].

OBJECTIVE: To evaluate the roles of bcl-2, bax gene and programmed cell death(PCD) in the TMJ development. METHODS: PCD, expression of both mRNA and protein of bcl-2 and expression of bax protein were observed by Tdt-mediated dUIP nick end labeling (TUNEL), mRNA in situ hybridization and immunohistochemical staining in TMJ of SD rats during series prenatal and postnatal developmental periods. RESULTS: PCD was involved in condylar cartilage in development of different periods. The cells of PCD were mainly located in proliferative zone and the area between prehypertrophic zone and late hypertrophic zone. bcl-2mRNA was expressed with high levels in proliferative and prehypertrophic zones and very few matured chondrocytes expressed bcl-2. The expression of bcl-2 protein showed the same distribution as bcl-2mRNA, but gradually decreased in late hypertrophic zone, compared with strong expression of Bax throughout condylar cartilage. CONCLUSIONS: PCD of condylar cartilage is regulated by bcl-2 and bax gene, the number and site of PCD are attributed to the change of bcl-2 and bax gene, and bcl-2 is an important gene in maintaining survival of chondrocytes during the proliferation, differentiation and maturation.

Animals↗
PubMed2000

[Study on molecular mechanism of ganyang shangkang syndrome in hypertension].

OBJECTIVE: To investigate the molecular mechanism of Ganyang Shangkang (Liver Yang ascending, GYSK) syndrome in hypertension. METHODS: The plasma norepinephrine (NE), epinephrine (E) contents in patients with GYSK syndrome or Ganshen Yinxu (Liver and Kidney Yin Deficiency, GSYX) syndrome who were suffered from hypertension, and normal controls were determined by high performance liquid chromatography-electrochemical detector (HPLC-ECD). The polymorphism of tyrosine hydroxylase (TH) gene in the three groups were analyzed by Southern Blot assay. The polymorphism of TH and MAOA, MAOB gene microsatellite in these groups were analyzed by polymerase chain reaction (PCR)-SSCP; meanwhile, the GYSK model by administration of single TCM preparation, Radix Aconiti preparate (RAP) in spontaneous hypertension rats (SHR). The adrenal gland medulla were examined for their TH expression at the protein level after ABC immuno histochemical staining with the TH monoclonal antibody (McAb). The adrenal TH gene mRNA expression was demonstrated by in situ hybridization with the synthesized TH oligonucleic acid probe. The results of immunohistochemistry and in situ hybridization were analyzed with the image analysis system (IAS). RESULTS: The plasma NE, E contents in patients with GYSK were more significantly increased than that in GSYX and normal controls. The TH gene amplified and the type A1 TH microsatellite D11S4046 was increased more remarkably than other groups. The TH mRNA protein expression in their adrenal tissue of the single TCM preparation RAP induced GYSK model in SHR were elevated. CONCLUSIONS: The GYSK syndrome of hypertension possesses the character as amplified TH gene and increased TH mRNA and protein. It suggested that the hyperexpression of TH is probably the mechanism of GYSK syndrome in hypertension.

Adult↗
PubMed2000

[Influence of safflower injection on hemorheology of patients of chronic cor pulmonale during acute attack].

OBJECTIVE: To study the effect of safflower injection (SI) on hemorheology and fibrinolytic system in treating patients of chronic cor pulmonale during acute attack. METHODS: Sixty patients were divided into two groups, 30 in each group. Both groups were treated with conventional therapy including anti-inflammatory, relieving cough and reducing sputum, cardial, diuretic and continuous low-flow oxygen inhalation. For the SI group, additional two courses of SI injection was given intravenously for 10 days as one course with an interval of 5 days between courses. The clinical therapeutic effect in the two groups and effect of treatment on hemorheology and fibrinolytic function were observed. RESULTS: The total effective rate in the SI group was 86.7% and that in the control group was 63.3%, the difference between the two groups was significant (P < 0.05). After treatment, in the SI group, the high- and low-shear whole blood viscosity, plasma viscosity, erythrocyte agglutination index, hematocrit and fibrinogen lowered obviously, as compared with before treatment (P < 0.01 or P < 0.05), and the improvement in hemorrheologic property was better than that in the control group significantly (P < 0.05). The blood activities of anti-thrombin III, plasminogen and tectotype profibrinolytic activating factor were all higher than those in the control group (P < 0.05, P < 0.01). And SI showed more potent effect in improving anti-coagulation and fibrinolytic function of patients than that of the control (P < 0.05). CONCLUSION: SI is an effective drug for treatment of hypercoagulability status in acute stage of chronic cor pulmonale, and is worthy of clinical application.

Aged↗
PubMed2000

[Clinical study on endometrial ovarian cyst treated by integrated laparoscopy and Chinese herbal medicine].

OBJECTIVE: To investigate a new method of integrated laparoscopy with Chinese herbal medicine (CHM) in treating endometrial ovarian cyst. METHODS: One hundred and fifty-two patients with endometrial ovarian cyst were randomly treated with three therapies: Integrated laparoscopy with CHM (combination group), CHM (TCM group) and Danazol (WM group). To compare the clinical efficacy and side-effects of the three groups and to elucidate the therapeutic mechanism by detecting the serum CA125 reproductive endocrine hormone, plasma PGF1 alpha, PGE2 etc. RESULTS: The shrinking rate and disappearance rate of the cysts, and markedly effective rate of combination group were obviously higher than those of TCM group and WM group (P < 0.05, P < 0.01). The pregnancy rate of combination group was also higher than that of WM group (P < 0.05). Few side-effects were the most prominent advantage for combination group and TCM group. After treatment, the plasma PGF1 alpha lowered remarkably, serum p.o. increased and CA125 reduced obviously (P < 0.01, P < 0.05). CONCLUSION: The integrated laparoscopy and CHM combination therapy is a new method in treating endometrial ovarian cyst with significant efficacy, minimal adverse effect and maximal preservation of reproductive function.

Adult↗
PubMed2000

[Expression of telomerase genes in human tumors].

OBJECTIVE: To investigate the correlation between the expression of telomerase genes and malignant phenotypes of human tumors and to compare the expression of telomerase genes and its activity reported in order to evaluate the role of detection of telomerase genes in tumor diagnosis. METHODS: With in situ hybridization, the expression and distribution of telomerase hTR and hTRT genes were observed in 78 cases of human cancer tissues, 20 cases of precancerous lesions and 28 of benign lesions. The results were statistically analyzed. RESULTS: hTR and hTRT were detected in 85% (66/78) and 82% (64/78) of the primary cancers. While in the adjacent tissues, the positive rates were only 3% (2/78) and 5% (4/78). In 20 precancerous cases, the positive rate for hTR and hTRT were 20% (4/20) and 15% (3/20) and the positive cases in 28 benign lesions were 0 and 1/28 (4%), respectively. The positive detection of hTR and hTRT expression in cancer group were significantly different from those in the adjacent tissues, precancerous cases and benign lesion group (P < 0.01). In analysing main types of the human cancers, the positive frequency of hTR and hTRT were 94% (15/16) and 88% (14/16) for the breast cancer, 85% (17/20) and 90% (18/20) for the colon cancer, 80% (8/10) and 80% (8/10) for the gallbladder cancer, 75% (6/8) and 75% (6/8) for the lung cancer, 75% (6/8) and 75% (6/8) for the stomach cancer, and 83% (5/6) and 83% (5/6) for the esophagus cancer, respectively. The expression level of telomerase hTR and hTRT correlated well with the malignancy and metastatic potentials in breast cancer, colon cancer and bladder cancer. Besides, the expressions of hTR and hTRT were noticed to be also highly correlated (P < 0.01). CONCLUSIONS: The expression of telomerase genes correlates with tumor malignant phenotypes, and may reflect the progression of tumors, and the detection of telomerase gene expression may be useful in a retrospective cancer research. It is worthwhile for further study to clarify whether screening of telomerase gene expression is able to become a new index for tumor diagnosis and prognosis.

Breast Neoplasms↗
PubMed2000

[An experimental study on cerulenin induced apoptosis of human colonic cancer cells].

OBJECTIVE: To investigate whether cerulenin could induce apoptosis and suppress the proliferation of human colonic cancer cells. METHODS: Cell morphology, microculture tetrazolium assay (MTT), agarose gel electrophoresis and flow cytometry assays were performed to identify the inhibitory effect on cell proliferation and the induction of apoptosis in human colonic cancer cells (LoVo). RESULTS: Cerulenin was able to induce LoVo cells death via apoptosis. Cerulenin also inhibited the proliferation of LoVo cells in vitro and was dose-dependent. Nuclear DNA of apoptotic cells displayed ladder bands characteristic of internucleosomal DNA fragmentation and apoptotic peak. The inhibitory effect of cerulenin on proliferation of LoVo cells was associated with cell cycle arrest from S to G(2)-M phase transition and with induction of apoptosis. In contrast, cerulenin could not affect the proliferation of human fibroblasts in vitro. CONCLUSION: The fatty acid synthase inhibitor, cerulenin, enables to induce cell apoptosis and to suppress the growth of human colonic cancer cells by inhibition of the synthesized fatty acids endogenously in the cancer cells.

Apoptosis↗
PubMed2000

[Cerulenin inhibits growth of human colonic carcinoma in nude mice].

OBJECTIVE: To determine the effects of fatty acid synthase inhibitor, cerulenin, on tumor growth of human colonic carcinoma (LoVo) in nude mice. METHODS: The transplantable colonic carcinoma cell line LoVo model was established in BALB/CA-nu/nu mice by subcutaneous implantation. Cerulenin was administrated by i.p. injection (80 mg/kg, 160 mg/kg each time) for 10 times. 17 days later the mice were killed and tumor size evaluated. RESULTS: The size of tumors in cerulenin-treated group were significantly smaller than in control group and were similar to that of the 5-Fu treated group. Transmission electronic microscopy (TEM) revealed that death of LoVo cells treated by cerulenin was associated with apoptosis characterized by cell shrinkage, condensation and chromosome fragmentation. bcl-2 oncoprotein expression rate was significantly lower in cerulenin-treated group in comparing with the control group. The expression rate of bax was the opposite to that of bcl-2. CONCLUSIONS: Cerulenin effectively suppresses the growth of human colonic carcinoma cells in vivo. The mechanism of cell death may be correlated with apoptosis, and bcl-2 and bax gene may play an important role in regulating cerulenin-induced apoptosis.

Animals↗