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Biomedical subjects

Y Inoue

Publications and source records attributed to Y Inoue.

At least 1,639 records · Page 91Linked to original sources
PubMed1988

[Superior vena cava syndrome associated with carcinoma of the uterine cervix--report of two cases].

Superior vena cava syndrome (SVCS) is a very rare complication in patients with a gynecologic malignancy. Only 4 cases have been reported in the English literature thus far and, to our knowledge, no such case has been reported in the Japanese literature. We have encountered two cases of SVCS in patients with cervical cancer. In case 1, the SVCS developed secondarily to a metastasis of the cervical cancer to the lungs and mediastinum. In case 2, the cause of the SVCS had been presumed to be due to metastatic cervical cancer, however on autopsy, it turned out to be due to a coexistent malignant lymphoma.

Carcinoma, Squamous Cell↗
PubMed1988

[Clinical results and problems of total-body thermochemotherapy].

The clinical results and problems of extracorporeally-induced total-body thermochemotherapy (TBHT) for recurrent cancer are presented. A total of 127 hyperthermic treatments were performed in 45 patients who had undergone unsuccessful conventional systemic anticancer chemotherapy. Partial response was observed in 11 of 34 evaluable patients (32%). In analysing the anticancer effects of TBHT according to cancer site, a high efficacy was observed in patients with their main tumor in the lung, liver and lymph nodes. The anticancer effects were most enhanced when TBHT was performed in combination with cisplatin and 5-fluorouracil. In order to augment the anticancer effects of TBHT, the choice of combined agents and administration timing are important. A useful method for determining the thermochemosensitivity of individual cancer cells to agents selected for drug treatment is the human tumor clonogenic assay. Furthermore, the usefulness of angiotensin II-induced hypertensive chemotherapy during TBHT for augmenting selective drug delivery to cancer tissue is stressed.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1988

[Magnetic resonance imaging (MRI) of intracranial chordomas].

MR images of 5 patients with intracranial chordoma were evaluated and compared with those of other clival lesions (1 clival osteomyelitis, 1 metastatic clival tumor, 3 clival meningiomas). The MR examination was performed using a 0.5 T superconductive magnet, with approximately 10 mm section thickness, one average and a 256 x 256 matrix. T1 weighted images were obtained by inversion recovery (IR) with TR 2100-2500 msec, TI 600 msec and TE 40 msec. T2 weighted images were obtained by spin echo pulse sequence with TR 1800-2500 msec and TE 120 msec (long SE). In several cases, the spin echo pulse sequences with TR 1000 msec and TE 40 msec (short SE) were also done. Multiplaned images were obtained. Four of 5 intracranial chordomas were low in intensity compared to cerebral gray matter on T1 weighted images, and all of 5 chordomas were as high in intensity as cerebrospinal fluid or higher than that of cerebrospinal fluid on T2 weighted images. Clival fatty marrow is high intensity on T1 weighted images. Clival involvement by a tumor was a clearly demonstrated as disappearance of this high intensity in all cases. In two cases, the tumor extended to the retropharyngeal space and this was detected clearly on short SE image. Although clival fatty marrow was disappeared, osteomyelitis and metastatic tumor in clivus were iso-intense to cerebral gray matter on both T1 and T2 weighted images.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1988

Corneal endothelial changes associated with herpetic stromal keratitis.

Wide-field specular microscopy was performed on both eyes of 33 patients with unilateral herpetic stromal keratitis. Endothelial changes were quantitated by computerized morphometric analysis of individual cells. In the 16 patients with disciform keratitis, the corneal endothelium of the affected eyes showed no difference in cell density but demonstrated significant increases of variation in cell size (polymegathism) and shape (pleomorphism) when compared to the cells in the fellow unaffected eyes. The eyes with keratouveitis (17 patients), however, had marked polymegathism and pleomorphism of the endothelium and a distinctly lower endothelial cell density (mean, 19%) than the healthy fellow eyes.

Adolescent↗
PubMed1987

Light microscopy of GTP-binding protein (Go) immunoreactivity within the retina of different vertebrates.

To examine species differences in the distribution pattern of guanosine triphosphate (GTP)-binding protein (Go) within the vertebrate retina, paraffin-embedded retinae from a number of vertebrate species, including the goldfish, frog, turtle, chicken, monkey, and human, were immunohistochemically stained with affinity-purified antibody against the alpha-subunit of Go. Go-immunoreactive products were found to be located in the neuropil, but not in the cell bodies of neurons, in the retina of all these species. However, some species differences were observed. In the frog, monkey and human, the inner plexiform layer (IPL) was homogeneously stained with this antibody, but in the goldfish, turtle and chicken, the IPL was heterogeneously stained. In the frog, chicken, turtle and human, the outer plexiform layer (OPL) was densely stained with this antibody, but in the goldfish and monkey, the OPL was rather faintly immunoreactive to the antibody. In the goldfish, monkey and human, the outer nuclear layer (ONL) was not immunoreactive to the Go-antibody, whereas in the frog, turtle and chicken, the ONL was immunoreactive to it. The implications of these species differences in Go localization in the vertebrate retina are discussed.

Animals↗
PubMed1987

Identification and determination of absolute and anomeric configurations of the 6-deoxyaltrose residue found in polysialoglycoprotein of Salvelinus leucomaenis pluvius eggs. The first demonstration of the presence of a 6-deoxyhexose other than fucose in glycoprotein.

An oligosaccharide alditol, dHex-GalNAc-Gal-Gal-GalNAcol, has been isolated from polysialoglycoprotein, which was derived from the unfertilized eggs of Savelinus leucomaenis pluvius (a salmonid fish, Iwana in Japanese), by alkaline borohydride treatment followed by exhaustive digestion with sialidase. First, the structure of the terminal dHex residue in the above pentasaccharide has been assigned as 6-deoxyaltrose (= dAlt in pyranoid form) by a combination of structural methods (GLC, TLC, mass spectrometry, and 400-MHz 1H-NMR spectroscopy). The occurrence of a 6-deoxyhexose other than L-fucose in glycoprotein has not been previously reported. Next, the absolute configuration of this unusual sugar residue has been assigned as D on the basis of the exciton-splitting study of tris-p-bromobenzoate derivatives of methyl 6-deoxyaltrosides. The usefullness of this circular dichroic exciton-splitting method in the determination of the absolute configuration of carbohydrate components, only available in minute amounts, is emphasized. The anomeric configuration of the glycosidic linkage of the D-altropyranosyl residue was deduced from 400-MHz 1H NMR spectroscopy. The 6-deoxy-beta-D-altropyranosyl residue thus established has the same configuration as alpha-L-fucose but with the C-5 methyl group inverted, suggesting that the biosynthetic incorporation of D-dAlt parallels that of L-fucose, and a possible pathway is also considered.

Animals↗
PubMed1987

Endocrine cells in pancreatic islets of Langerhans are immunoreactive to antibody against guanine nucleotide-binding protein (Go) purified from rat brain.

Guanine nucleotide-binding proteins (G proteins) are usually classified into four subclasses (Gs, Gi, Go and Gt or transducin). We localized the anti-Go immunoreactivities in islets of Langerhans of the rat pancreas by using affinity-purified antibody against the alpha-subunit of Go purified from rat brain. Endocrine cells of the islets of Langerhans were stained with this Go antibody, but the acinar cells in the exocrine portion of the pancreas were immunonegative to this antibody. These findings strongly suggest that Go protein functions as intermediaries in the transmembrane signalling pathway in the endocrine cells of the islets of Langerhans.

Adenylyl Cyclases↗
PubMed1987

Modification of the properties of S2 state in photosynthetic O2-evolving center by replacement of chloride with other anions.

Properties of the S2 state formed in photosystem II membranes in which Cl- had been replaced by various anions were investigated by means of thermoluminescence measurements and low temperature EPR spectroscopy. The Br--substituted membranes showed the normal thermoluminescence B-band arising from S2Q-B charge recombination, whereas the SO2-4-, F--, CH3COO--, and NO-3-substituted membranes showed modified B-bands with variously upshifted peak temperatures. The extent of the peak temperature upshift varied in parallel with the extent of inhibition of O2 evolution depending on the anion species. A normal EPR S2 multiline signal was induced in Br--substituted membranes, but its amplitude was reduced to less than 10% in F--, NO-3-, CH3COO--, and SO2-4-substituted membranes, In contrast, the g = 4.1 signal from S2 was markedly enhanced in F-- and NO-3-substituted membranes, not much affected in CH3COO-- and SO2-4-substituted membranes, and decreased to 70% in Br--substituted membranes. Based on these data, the effect of various types of S2 modification on the O2-evolving activity was discussed. It was suggested that anions have an important role in regulating the interaction between the Mn atoms, and thereby adjust the redox properties of the S2 state to enable further transitions beyond S2.

Anions↗
PubMed1987

Substitution of an arginyl residue for the active site lysyl residue (Lys258) of aspartate aminotransferase.

The active site lysyl residue (Lys258) of E. coli aspartate amino transferase was substituted for an arginyl residue by oligonucleotide-directed, site-specific mutagenesis. The mutant enzyme was obviously unable to form an aldimine bond with pyridoxal 5'-phosphate but firmly bound the coenzyme. The finding that the mutation did not lead to entire loss in the enzymic activity suggests that Lys258 may not be essential but auxiliary for enzymic catalysis. It is also conceived that the positive charge provided by Arg258 may contribute to the enzymic catalysis.

Amino Acid Sequence↗