Search PubMed⌕ Search

Biomedical subjects

Y Inoue

Publications and source records attributed to Y Inoue.

At least 1,495 records · Page 83Linked to original sources
PubMed1990

[Therapeutic results and pharmacokinetics of combined used anticancer drug in intraperitoneal hyperthermo-chemotherapy (CHPP)].

Continuous hyperthermic peritoneal perfusion (CHPP) was performed after curative gastrectomy for the patients with serosally invading gastric cancer to control their peritoneal recurrence. In the randomized control study of this prophylactic CHPP, using 10 l of physiological saline with 100 mg of mitomycin C or 150 mg of cisplatin (CDDP) at 42 degrees C, better post-operative survival was obtained in the CHPP group than the control group, two-year survivals were 83% in the CHPP and 70% in the control. Through the evaluation of time-concentration curve of free-Pt in serum, the side effects of CDDP in the CHPP treatment were considered to be almost equal to that of 25 mg of CDDP in the intravenous injection. In the experiments using rats, the concentration of Pt in the peritoneal tissue after CHPP was three times higher than that after intravenous injection of 2 mg of CDDP. These three results, better survival, less side effects and higher drug concentration of the peritoneal tissue, support the reasonableness of CHPP.

Animals↗
PubMed1990

Estimation of total oxygen uptake in women during exercise by total heart beats and aerobic fitness.

The present study was performed to find a practical method for estimating total O2 uptake (TVO2) of women during exercise based on total heart beats (THB) and aerobic fitness level, and examine the influence of the type of exercise on the estimation. After 60 observations on 20 female subjects tested by the cycle ergometer, the following formula was derived. TVO2 (ml.kg-1) = SR125 X (61.0 X mean HR + 2543) X THB X 10(-4), where mean HR is mean heart rate (beats.min-1) in exercise, and SR125 is the slope of the regression line between accumulated heart beats and accumulated O2 uptake during exercise at 125 beats.min-1 of mean HR. SR125 was significantly correlated not only to VO2max but also each score (X) in any simple endurance tests, such as the step test for 3 min, yielding a formula, SR125 = -0.00115X + 0.3081. Both formulae indicate that the TVO2 of any exercising person can be estimated from THB and mean HR when SR125 was determined by the simple endurance test. The discrepancy between both TVO2 as estimated by our method and measured directly by the Douglas bag method during walking on a treadmill was not significant with that during the cycling on an ergometer. Accordingly, our method may possibly be used for estimating TVO2 in exercise mainly using the leg muscles such as in cycling and walking.

Adult↗
PubMed1990

[Evaluation of CT-guided stereotaxic aspiration for thalamic hemorrhage by single photon emission CT].

Sixteen patients with thalamic hemorrhage (hematoma volume greater than or equal to 10 ml and CT classification greater than or equal to IIa) were included in this study. In sixteen patients, ten were treated conservatively (conservative group) and six were subjected to CT-guided stereotaxic aspiration (aspiration group). Stereotaxic aspiration was performed at the acute stage within five days after the onset. We measured cerebral blood flow (CBF) using Single Photon Emission CT (Tomomatic 64) and 133-Xe inhalation method. In both groups, CBF was measured at the onset (day 1-5), acute stage (day 7-13), subacute stage (1 month from the onset) and chronic stage (2-4 months from the onset). In this study, mean hemispheric cerebral blood flow (mCBF) was calculated from the middle slice 2, positioned 5cm above the orbitomeatal line. Regional devision of slice 2 in the hematoma side was made as follows. rCBF-A: regional cerebral blood flow in the anterior area of the hematoma. rCBF-L: regional cerebral blood flow in the lateral area of the hematoma. rCBF-P: regional cerebral blood flow in the posterior area of the hematoma. In both groups, age, neurological grading, CT classification and hematoma volume had no significance. In the conservative group, mCBF of the hematoma side in the subacute stage was 68 +/- 7%, and in the aspiration group it was 85 +/- 17%. This difference was statistically significant.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1990

[A case of ectopic large pituitary adenoma].

Ectopic pituitary adenomas are very rare and only 17 cases have been reported. In this paper we present a case of large pituitary adenoma originating in the suprasellar region. A 26-year-old man was admitted to our clinic with a chief complaint of headaches. Neurological examination revealed slight disorientation and bilateral choked disk. Hormonal study revealed that the serum prolactin level was 3300ng/ml and serum growth hormone level was 29.5ng/ml. Computed tomography showed a large mass in the suprasellar region extending upward to the third ventricle and backward to the pons. T1-weighted MR imaging revealed that the intensity of the mass was the same as that of the cerebral cortex and the pituitary gland was showing high intensity in the pituitary fossa. The tumor was radically removed via the transpetrosal transtentorial approach. Histologically, the tumor was a prolactin-growth hormones producing pituitary adenoma. The literature was reviewed and the origin of the tumor was discussed.

Adenoma, Chromophobe↗
PubMed1989

Sexual response in Saccharomyces cerevisiae: alteration of enzyme activity in the glyoxalase system by mating factor.

Glyoxalase I activity in alpha-type budding yeast of the Saccharomyces cerevisiae strain was increased by exposure of alpha-type cells to supernatant of a culture of a-type yeast cells, although glyoxalase II activity was decreased by the same treatment. The alteration of enzyme activity in the glyoxalase system occurred during the 30-60 min period after exposure of alpha-type cells to a-type culture supernatant. No change of glyoxalase I and II activities was found in the case of the alpha-type strain, S. cerevisiae VQ3 (alpha ste3-1), which is deficient in a-factor receptors.

Alcohol Oxidoreductases↗
PubMed1989

Isolation and structures of glycoprotein-derived free sialooligosaccharides from the unfertilized eggs of Tribolodon hakonensis, a dace. Intracellular accumulation of a novel class of biantennary disialooligosaccharides.

Novel acidic oligosaccharides were isolated in abnormally large amounts (about 200 ng/egg) from the unfertilized eggs of Tribolodon hakonensis (a dace, "ugui" in Japanese). The free oligosaccharides were found to consist of a mixture of disialylated species most of which end with beta-mannosyl N-acetylglucosamine structure at their reducing termini, i.e. greater than Man beta 1-4GlcNAc. A minute portion of the sialooligosaccharides was found to have the reducing terminal structure, di-N-acetylchitobiose, i.e. greater than Man beta 1-4GlcNAc beta 1-4GlcNAc. From the structural analysis of these free sialooligosaccharides, the following structures are proposed: (sequence; see text) Occurrence of such a symmetrically or dissymmetrically branched form of the biantennary nonreducing periphery as revealed here is novel. Although it is unknown why and how such high amounts of free oligosaccharides are accumulated in unfertilized eggs, these were presumably protein-linked components and must be released at certain stages of oogenesis.

Animals↗
PubMed1989

Isolation and characterization of deaminated neuraminic acid-rich glycoprotein (KDN-gp-OF) in the ovarian fluid of rainbow trout (Salmo gairdneri).

A novel highly acidic glycoprotein (deaminated neuraminic acid-rich glycoprotein; KDN-gp) was first discovered as an integral component of the vitelline envelope of rainbow trout eggs [Inoue, S., et al. (1988) Biochem. Biophys. Res. Commun. 153, 172-176]. Another member of this class of glycoprotein has now been found in the ovarian (or coelomic) fluid of ovulating rainbow trout. This ovarian fluid KDN-glycoprotein is designated as KDN-gp-OF and its amino acid and carbohydrate compositions were compared with those of the vitelline envelope KDN-gp (KDN-gp-VE). KDN-gp-OF was similar to KDN-gp-VE in the carbohydrate composition and molecular weight. However, a small but definite difference in amino acid composition and the molecular weight range was found between KDN-gp-OF and KDN-gp-VE. The results suggest that in KDN-gp-OF some peptide sequences presumably present at either the C- or N-terminus are deleted from KDN-gp-VE. Possible biological function of KDN-gp-OF is discussed.

Amino Acids↗
PubMed1989

Determination of bromperidol in serum by automated column-switching high-performance liquid chromatography.

A column-switching high-performance liquid chromatographic method with ultraviolet detection is described for the simple and rapid determination of bromperidol, a neuroleptic drug, in dog and human sera. The drug in serum and trifluperidol added to the serum as an internal standard were extracted with hexane-chloroform, and the extract was subjected to automated column-switching high-performance liquid chromatography using a hydrophilic metaacrylate polymer column (TSK gel PW precolumn) for sample clean-up and a reversed-phase column (TSK gel ODS-80TM) for separation. The detection limit of bromperidol is 0.3 ng/ml serum and the recovery of bromperidol added to serum (2.7-16.0 ng/ml) was satisfactory with a standard deviation of 3% or less.

Adult↗
PubMed1989

[MR images of wallerian degeneration--relation between the time and MR findings of wallerian degeneration].

On magnetic resonance (MR) images, wallerian degeneration of the pyramidal tract can be demonstrated as an abnormal intensity showing prolonged T1 and T2 relaxation times corresponding to the corticospinal tract, and/or as shrinkage of the ipsilateral cerebral peduncle and pons. We evaluated 150 cases with supratentorial cerebrovascular accident (CVA) and found the above findings in 35 patients (hematoma 21, infarction 14). The time interval between the ictus of CVA and manifestation of wallerian degeneration on MR images was evaluated. In all 35 cases the foci of CVA located at or extended into the motor cortex, the corona radiata of the pyramidal tract or the posterior limb of the internal capsule. Abnormal signal intensity appeared as early as 5 weeks after ictus and was observed in all 17 cases after 10 weeks. Ipsilateral shrinkage of the cerebral peduncle and the pons has appeared 13 months after ictus. MR appears to be the most useful modality for early detection of waller degeneration.

Cerebrovascular Disorders↗
PubMed1989

Structural studies of fertilization-associated carbohydrate-rich glycoproteins (hyosophorin) isolated from the fertilized and unfertilized eggs of flounder, Paralichthys olivaceus. Presence of a novel penta-antennary N-linked glycan chain in the tandem repeating glycopeptide unit of hyosophorin.

New glycoproteins of 100-120 kDa were isolated from the unfertilized eggs of flounder, Paralichthys olivaceus. Compositionally indistinguishable glycopeptides of 6 kDa were also purified from the activated or fertilized eggs. These high and low molecular mass glycoproteins are characterized by high (about 85%) carbohydrate content. Although some heterogeneities exist in the amino acid sequences, the 6-kDa glycopeptides (decapeptides with single large N-linked glycan chains), isolated from the fertilized eggs are the repeating units of the high molecular mass glycoproteins. As judged from several distinctive features the 100-120-kDa glycoproteins are apparently major components of cortical alveoli of flounder eggs and are regarded as members of glycoproteins we have defined under the name of "hyosophorin" (Kitajima, K., Inoue, S., and Inoue, Y. (1989) Dev. Biol. 132, 544-553). Composition analysis, Smith degradation, hydrazinolysis-nitrous acid deamination, permethylation analysis, and 400-MHz 1H NMR spectroscopy provided evidence for the structure of a novel penta-antennary glycan chain attached to the repeating unit (decapeptide) of the protein core. The structure thus determined is: (Formula: see text). The presence of a unique class of carbohydrate-rich glycoproteins (H-hyosophorin) in the unfertilized eggs, their conversion to the repeating unit (L-hyosophorin) at fertilization, and the finding of a free glycan chain that was formed by scission between the GlcNAc and Asn residues of L-hyosophorin, in the fertilized eggs including embryos of 4-11-h postinsemination, support the view that these molecules may be important in fertilization and subsequent development.

Amino Acid Sequence↗
PubMed1989

Cleavage of a synthetic COOH-terminal oligopeptide of D1 precursor protein by a purified processing enzyme.

A synthetic COOH-terminal oligopeptide of D1 protein deduced from the spinach psbA gene (Asn-325-Gly-353) was subjected to proteolytic digestion by purified processing enzyme of D1 protein [(1989) FEBS Lett. 246, 218-222] and the following two fragments were obtained as cleavage products: a COOH-terminal 9-amino-acid fragment (Ala-345-Gly-353) and an NH2-terminal 10-amino-acid fragment (Asn-325-Arg-334). It was concluded that: (i) the oligopeptide consisting of the COOH-terminal 29-amino-acid sequence deduced from the spinach psbA gene provides the recognition domain for the processing enzyme; (ii) the cleavage takes place at the predicted processing site of native D1 precursor protein (COOH side of Ala-344); and (iii) another cleavage takes place at an additional site (COOH side of Arg-334) for the synthetic substrate, but not for the native D1 precursor protein.

Amino Acid Sequence↗
PubMed1989

N-terminal sequencing of low-molecular-mass components in cyanobacterial photosystem II core complex. Two components correspond to unidentified open reading frames of plant chloroplast DNA.

We recently reported the presence of several low-molecular-mass protein components in the PS II O2-evolving core complex from the thermophilic cyanobacterium, Synechococcus vulcanus [(1989) FEBS Lett. 244, 391-396]. Here we have characterized the three components (4.1, 4.7, 5 kDa) of the same cyanobacterial core complex by N-terminal sequencing. There were two components in the 4.7 kDa region, both having a blocked N-terminus. One has a sequence highly homologous to open reading frame 34 of plant chloroplast DNA (tentatively designated psbM), while the other has a sequence partially homologous to open reading frame 43 of chloroplast DNA (designated psbN), although neither of the two gene products has yet been confirmed in chloroplasts. The cyanobacterial 4.1 kDa protein partially corresponds to the 4.1 kDa nuclear-encoded core component of higher plant PS II. The cyanobacterial 5 kDa component, however, shows a sequence that is unrelated to any other known proteins.

Amino Acid Sequence↗
PubMed1989

Identification of photosystem I components from the cyanobacterium, Synechococcus vulcanus by N-terminal sequencing.

The photosystem I core complex isolated from a thermophilic cyanobacterium, Synechococcus vulcanus, is composed of eight low-molecular-mass proteins of 18, 14, 12, 9.5, 9, 6.5, 5 and 4.1 kDa in addition to the PS I chlorophyll protein. N-terminal amino acid sequences of all these components were determined and compared with those of higher plants. Clearly, the 9.5 kDa component corresponds to the protein which carries the non-heme iron-sulfur centers A and B. This protein is so poorly visualized by staining that it has probably been overlooked in gel electrophoresis analyses. The 18, 14, 12 and 9 kDa components show appreciable homology with respective subunits of higher plant PS I. In contrast, the 6.5, 5 and 4.1 kDa components do not correspond to any known proteins except that the sequence of the 4.1 kDa component matches an unidentified open reading frame (ORF) 42 (liverwort) or ORF44 (tobacco) of chloroplast DNA.

Amino Acid Sequence↗
PubMed1989

Substitution of a lysyl residue for arginine 386 of Escherichia coli aspartate aminotransferase.

Substitution of a lysyl residue for Arg-386 of Escherichia coli aspartate aminotransferase resulted in an extensive decrease in Vmax values (0.8% with the aspartate-2-oxoglutarate pair and 0.2% with the glutamate-oxalacetate pair, compared with the corresponding values for the wild-type enzyme). Kinetic analysis of the four sets of half-reactions, the pyridoxal form of the enzyme with aspartate or glutamate and the pyridoxamine form with 2-oxoglutarate or oxalacetate, allowed us to define the independent effect of the mutation on the reactivity of each substrate. Decrease in the first order rate constant (kmax) was more pronounced in the reactions with five-carbon substrates (glutamate and 2-oxoglutarate) than in those with four-carbon substrates (aspartate and oxalacetate), while the increase in the apparent dissociation constant (Kd) was greater for four-carbon substrates than for five-carbon substrates. The decrease of overall catalytic efficiency as judged by the values, kmax/Kd, was more pronounced in the reactions with five-carbon substrates than in those with four-carbon substrates. Affinities for substrate analogs such as succinate, glutarate, 2-methylaspartate, and erythro-3-hydroxyaspartate, were also considerably decreased by the mutation of the enzyme. These findings indicate that the side chain of the lysyl residue, although it bears a positive charge similar to that of the arginyl residue, is not structurally adequate for the productive binding of a substrate during catalysis.

Amino Acid Sequence↗
PubMed1989

Protein kinase C activity can desensitize the gonadotropin-responsive adenylate cyclase in Leydig tumor cells. But hCG-induced desensitization does not involve protein kinase C activation.

The murine Leydig tumor cell line, MLTC-1, contains a gonadotropin receptor-coupled adenylate cyclase. Although the binding of human choriogonadotropin (hCG) initially causes cells to accumulate cAMP, in time, the response to hCG is attenuated by desensitization. Treating intact cells with the tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), or with diacylglycerol also causes desensitization of the hCG response. These compounds are activators of calcium/phospholipid-dependent protein kinase (PKC). Treating MLTC-1 cells with TPA or dioctanoylglycerol increased the portion of PKC in the cell membrane fraction. This phenomenon is associated with activation of PKC. Treating isolated membranes with purified PKC desensitize the hCG response. Thus, desensitization caused by TPA or dioctanoylglycerol is probably mediated by PKC. PKC is normally activated when phosphoinositides are metabolized to diacylglycerol and inositol phosphates. There was no significant accumulation of inositol phosphates when cells were treated with hCG. hCG did not increase the portion of PKC in the cell membrane fraction. However, hCG could desensitize isolated membranes, but TPA could not. We conclude that although protein kinase C activity can desensitize the gonadotropin response, hCG does not cause desensitization by activating PKC. The implications of this observation are discussed.

Adenylyl Cyclases↗
PubMed1989

Determination of the functionally important heme peripheral vinyl group orientation in paramagnetic hemoprotein by 2D NMR.

2D NMR spectroscopies have been successfully used to characterize the heme peripheral vinyl groups in paramagnetic hemoprotein in spite of the difficulties from the rapid paramagnetic relaxation and the low digital resolution of the 2D NMR map. The scalar coupling network system among the vinyl protons is clearly identified in the COSY spectra from its characteristic cross-peak pattern and the dipolar coupling connectivities of the vinyl proton resonances with other heme side-chain proton resonances not only provide the specific assignment of vinyl beta-proton resonances but also allow the determination of the vinyl group orientation with respect to the heme plane.

Animals↗
PubMed1989

[Arg292----Val] or [Arg292----Leu] mutation enhances the reactivity of Escherichia coli aspartate aminotransferase with aromatic amino acids.

Arg292 of E. coli aspartate aminotransferase was substituted with valine or leucine by site-directed mutagenesis. In comparison with the wild-type enzyme, either of the mutant enzymes showed a decrease by over 5 orders of magnitude of kcat/km values for aspartate and glutamate. This supports the contention that Arg292 is important for determining the specificity of this enzyme for dicarboxylic substrates. In contrast, mutant enzymes displayed a 5- to 10-fold increase in kcat/Km values for aromatic amino acids as substrates. Thus, introduction of an uncharged, hydrophobic side chain into position 292 leads to a striking alteration in substrate specificity of this enzyme, thereby improving catalytic efficiency toward aromatic amino acids.

Amino Acid Sequence↗