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Biomedical subjects

Y Huang

Publications and source records attributed to Y Huang.

At least 271 records · Page 15Linked to original sources
PubMed2001

[An experimental study of the effects of thrombin receptor activating peptide (TP508) on healing of ischemic wound and flap survival in rats].

OBJECTIVE: To investigate the effects of the thrombin receptor activating peptide (TP508) on healing of ischemic wound and flap survival in rats. METHODS: Sixty-six Sprague-Dawley rats were employed as the model. On the back of the rats, three kinds of wound and flap were made to establish four groups as follows: partial ischemic wound in 16, full ischemic wound in 16, routine wound in 18 and flap in 16 rats. Each group was further divided into TP508 treating group and isotonic saline control group. The total and necrotic areas of the wounds and flaps were daplicated on acetate papers and calculated with a computer on the 3rd, 7th, 10th and 14th post-operation days (PODs). RESULTS: In routine wounds, the ischemic wound area treated by TP508 was 73.7% and 45.4% in saline control groups on 7 and 14 (PODs), respectively. While in the flap model, the necrotic flap area treated by TP508 was 80.4% and 56.8% in control groups on 7 and 14 (PODs), respectively. CONCLUSION: TP508 could accelerate healing of ischemic wound and improve flap survival in rats.

Animals↗
PubMed2001

[An experimental study on the apoptosis of PMNs and macrophages during the early postburn stage in severely scalded rats].

OBJECTIVE: To investigate the changes of postburn apoptosis of PMN and the apoptosis of PMN and macrophages after being stimulated by burn sera and the change of the phagocytosis of apoptotic PMNs by macrophages. METHODS: Rats inflicted by 30% TBSA III degree scalding were employed as the model. PMNs were harvested on the 0, 6, 12 and 24 postburn hour (PBH). Burn sera were harvested at 24 PBHs. PMN apoptosis was detected by flow cytometry. Simultaneously, peritoneal macrophages were collected for the study. The apoptotic change of the macrophages (Mphis) after being stimulated by burn sera and the ability of Mphis phagocytizing apoptotic PMNs at 24 PBHs were examined. RESULTS: The PMN apoptosis was obviously inhibited at 6, 12 and 24 PBHs in the scalded rats with no difference between the time spots. Twenty-four hours after being stimulated by burn sera, the apoptotic rate of PMNs on scalded rats was lower than that in normal rats, while the apoptotic rate of Mphis in scalded rats was higher than that in normal rats. The ability of Mphis phagocytizing apoptotic PMNs decreased after severe burn (P < 0.01). CONCLUSION: There exhibited delayed rat PMN apoptosis and decreased ability of the phagocytosis of apoptotic PMNs by the Mphis after severe burn or being stimulated by burn sera. This might lead to the incomplete phagocytosis of apoptotic PMNs by Mphis. As a result, the PMNs would finally come to necrosis and release toxic contents which were important factors in the inducing of SIRS.

Animals↗
PubMed2001

[New evidences for adapting Qian jin yao fang (Thousand golden essential prescriptions) by Office of Revising Medical Books of the Song dynasty].

Qian jin yao fang (Thousand Golden Essential Prescriptions) written by Sun Simiao in the Tang dynasty has two kinds of extant editions in circulation: one is the revised edition, Bei ji qian jin yao fang (Essential Prescriptions Worth a Thousand Gold for Emergencies) revised by the Office of Revising Medical Books of the Song dynasty, the other is the Song edition, Sun zhenren qian jin fang (Master Sun's Thousand Golden Prescription) (20 volumes extant) not revised by Song people. There are great discrepancies between these two editions not only in the order of chapter contents, including the order of items in the same chapter, but also in its texts. Concerning the causes of this difference, we already made preliminary analysis in our former paper. However, there are new evidences to further explain it with our recent research.

China↗
PubMed2001

[Study on psychoprophylaxis and monoamines neurotransmitter of patients with burning mouth syndrome].

OBJECTIVE: Burning mouth syndrome (BMS) is a chronic ache disease, usually occurring in middle aged and old women. This study sought to understand the psychopathologic aspect and monoamines neurotransmitters in the plasma of the patients with BMS. METHODS: Thirty cases were selected (26 females, 4 males); 30 normal control subjects were similar to the BMS cases on age and sex. All subjects were required to complete the Eysenck personality questionnaire (EPQ), and the Self-report Symptom Inventory, Symptom Check List-90 (SCL-90) questionnaire. In case a subject's L (lie) score exceeded 50, she (he) would be removed from the test. 2 ml of blood was drawn from the subject under restine conditions with a fast in the morning to examine norepinephrine and epinephrine contents by high efficient liquid chromatography. Chi-square test, analysis of variance and t'-test were performed. RESULTS: The BMS group had higher scores of nervousness (N) and poikilergasia (P) and lower score of extro/introversion (E) as compared with the control (P < 0.05). The personality types in BMS group were focused on introversion and instability, but in the control group the types were focused on extroversion and stability (P < 0.05). The scores of 9 emotional factors of BMS group were significantly higher than those of the control group (P < 0.05), which indicated that the BMS patients had suffered from serial psychic disorders. The level of plasma norepinephrine in the BMS patients was higher than that of the control (P < 0.01). CONCLUSION: The personality of BMS patients raised body response to harmful stimulations, and obvious psychic disorders in the patient may cause the functional disorders in central and sympathetic nervous systems, which may be associated with BMS' occurrence.

Adult↗
PubMed2001

[Determination of roxithromycin in serum by high-performance liquid chromatography with ultraviolet detection].

OBJECTIVE: To make better the RP-HPLC method for the determination of roxithromycin(RM) in human serum. METHODS: RM and clarithromycin (internal standard) were extracted from alkalinized serum sample (500 microliters) with methylene chloride. After evaporation of the organic layer, the residue was dissolved in 100 microliters of acetonitrile-ammonium phosphate (1:1, pH 6.0) and washed with n-hexane, then 20 microliters was injected onto a column (5 microns, 15 cm x 4.6 mm) of Penomenex luna C18. The mobile phase was acetonitrile-0.05 mol/L phosphoric acid (39:19:42, adjusted to pH 7.2 with ammonia water) pumped at 1.2 ml/min through the column. The variable wavelength UV detector operated at 0.01 aufs and the wavelength was set at 210 nm. RESULTS: The retention times for RM and clarithromycin were 4.4 min and 5.0 min, respectively. Standard curve was linear in the concentration range of 0.25 to 32 mg/L. The detection limit in serum was 0.06 mg/L; the average method recovery 97.4%; the inter-day RSD less than 3.0%. CONCLUSION: This method was found to be simple, rapid, sensitive and accurate for determination of RM in human serum.

Chromatography, High Pressure Liquid↗
PubMed2001

[p53 mutation in lung cancer in Gejiu and Xuanwei regions of Yunnan Province].

OBJECTIVE: To incidence rates of lung cancer among tin miners in Gejiu and among peasants in Xuanwei are higher than the average incidence rate in Yunnan Province, and the causes of the disease in these two regions have been preliminarily clarified. This study was directed to the molecular biological basis of its pathogenesis. METHODS: Twenty-six specimens of the lung cancers of Gejiu tin miners(GTM) and 15 ones of Xuanwei peasants (XP) were examined by single-strand conformation polymorphism analysis of polymerase chain reaction products. RESULTS: Using PCR primers for the most highly conserved region of the p53 including exons 5-8, we have identified p53 mutations in 13 lung cancer tumor DNA samples of GTM and in 4 ones of XP. Nucleotide sequencing of one of the exon 5 mutations of GTM indicated that there was a C to T transition, which resulted in substitution of Val for Ala at amino acid 135, and there was an AAC to TTC transertion in the exon 7 of XP, which resulted in substitution of Phe for Asn at amino acid 247. CONCLUSION: p53 mutation is a frequent incident in lung cancer of GTM and XP, and two new mutation sites were found in p53 gene, which have not been reported before now. There was no statistically significant correlation between the detection of p53 mutation and the patient's age, sex, mining history and histologic type of the cancer.

China↗
PubMed2001

[Simultaneous determination of chlorpheniramine, paracetamol and pseudo-ephedrine hydrochloride in composite tablets by high performance liquid chromatography].

An HPLC method is described for the determination of chlorpheniramine, paracetamol and pseudoephedrine hydrochloride simultaneously in Nuonuo composite tablets. Chromatographic conditions were: column, YWG-C18 column (150 mm x 4.0 mm i.d., 10 microns); mobile phase, 25% methanol(0.05 mol/L sodium dihydrogen phosphate); flow rate, 1.0 mL/min; detection wavelength, 205 nm; injection volume, 20 microL. The detection limits of chlorpheniramine, paracetamol and pseudo-ephedrine hydrochloride were 1.16 mg/L, 0.15 mg/L and 1.82 mg/L respectively and the corresponding average recoveries were 98.35% (n = 5, RSD = 1.60%), 101.16% (n = 5, RSD = 1.50%) and 98.50% (n = 5, RSD = 1.59%) respectively. The proposed method is simple, rapid and accurate and has been satisfactorily used for the quantitative analysis of real samples.

Acetaminophen↗
PubMed2001

[Extraction of DNA from environmental samples and construction of mixed genomic DNA libraries].

A method has been developed for extracting and purifying genomic DNA from environmental samples. In this method, an environmental sample is treated first by grinding and freezing/thawing and subsequently by SDS/proteinase K-based DNA extraction. The yields of purified DNA from three samples used in this study ranged from 2 to 16 micrograms per gram of dry sample. Mixed genomic DNA libraries for two of the environmental samples were constructed by inserting restriction fragments (3-8 kb) of the purified DNAs into plasmid pUC18 and transforming E. coli DH5 alpha with the resultant plasmids. Approximately 10(3) to 10(4) insert-containing clones were obtained from 1 g of each sample. Clone libraries were analyzed by DNA sequencing and gene annotation. Among 20 randomly-selected clones, 14 contained an insert whose sequence had not been reported while the rest had an insert of either E. coli or vector origin. A search of sequence databases using the end sequences of each of the foreign inserts showed that each sequence was part of a gene encoding, in most cases, a predictable function. Our results are of significance to the collection, investigation and exploitation of the genes of uncultured microorganisms.

Amino Acid Sequence↗
PubMed2001

[Study on the cytochrome P450 activity in alkane converting process of Candida tropicalis].

A method of reduced CO-difference spectrum was established to study the cytochrome P450 activity of the whole cell of Candida tropicalis during the alkane converting process. Using this method, the cytochrome P450 activities of the whole cells that were cultured in the different concentrations of alkane were studied. The results showed that the 5% alkane could induce the cytochrome P450 activity obviously but not inhibit the growth of cells, so it was determined preliminarily that the alkane concentration of the seed medium was 5%. The cytochrome P450 activities of dicarboxylic acid (DCA) fermentation processing were further studied. During the exponential phase of growth, the cytochrome P450 activity increased smoothly. However, during the phase of production of dicarboxylic acid, the cytochrome P450 activities increased rapidly after a sort decrease. The results still showed that the rate of production of dicarboxylic acid increased with the cytochrome P450 activity.

Alkanes↗
PubMed2001

[Genomic structure and proteins sequence analysis of full-length of segment A of three infections bursal disease viruses].

The full-length of segment A of three infectious bursal disease viruses (IBDV), including an attenuated strain HZ2, an attenuated vaccine strain JD1 and a virulent field isolate ZJ2000, were cloned by long RT-PCR and sequenced respectively. All the three results revealed the identity of IB-DV with two overlapping open reading frames (ORF) flanked by 5'- and 3'-noncoding regions(NCR) in 3259 bp long. The strains shared high identity with each other at nucleotide or deduced amino acid level, and also had four unique sites H253, N279, T284, R330 which are common in other attenuated and some classic or highly virulent strains. The virulent strain ZJ2000 had several key amino acid mutations located in hypervariant region of VP2 and near the VP2-VP4 cleavage site of polypeptide, which is probably related to the virulence. Sequence comparison supported that VP2 is not the sole determinant of the virulence. The highly conservation in 5'- and 3'-NCR of different strains indicated the NCR may be not responsible for the virulence. But the same conservation appeared in VP5 revealed another complex relationship between VP5 and the virulence.

Amino Acid Sequence↗
PubMed2001

The clinical study of Healon 5 in phacoemulsification and IOL implantation.

PURPOSE: To compare a new ophthalmic viscoelastic device (OVD) Healon 5 with two other kinds of OVDs commonly used in China during phacoemulsification and intraocular lens (IOL) implantation in terms of removal time, intraocular pressure, corneal and anterior chamber response. METHODS: This prospective randomized study, in which patients and observers are masked, comprises niety eyes. They were randomly divided into 3 groups with different OVDs. Healon 5 (sodium hyaluronate 5,000 2.3%) was compared with Healon GV (sodium hyaluronate 7,000 1.4%) and Iviz (sodium hyaluronate 1.0%). The surgeries were performed with temporal corneal incision, phacoemulsification in situ and foldable lens intracapsular implantation. The characteristic and the removal time of the viscoelastic material as well as the postoperative IOP, corneal reaction and anterior chamber reactions of the eyes were observed preoperatively and 24 hrs postoperatively. Exclusion criteria were glaucoma, a preoperative dilated pupil diameter smaller than 5.0 mm, proliferative diabetic retinopathy, significant corneal pathology or a history of uveitis. RESULTS: The removal time was 47.42 +/- 13.09 seconds in Healon 5, 16.50 +/- 5.45 in Healon GV and 15.83 +/- 7.40 in Iviz. The removal time of Healon 5 group was significantly longer than that of the other groups (P = 0.000). There is no significant IOP change 24 hours postoperatively and the difference of intraocular pressure among three groups was not significant (P > 0.05). Healon 5 group had the slightest corneal and anterior chamber response, followed by Healon GV and Iviz. CONCLUSION: Although takes longer time to remove, Healon 5 is an ideal viscoelastic material for its superior space maintenance capacity and high dispersity which provides superior protection of endothelial cells compared to Healon GV and Iviz.

Anterior Chamber↗
PubMed2001

[Application of corepraxia in the implantation of intraocular lens].

PURPOSE: In the cases of aphakia or cataract which complicated with iridosteresis, corectasis and pupil displacement, corepraxia was performed when implanting intraocular lens (IOL) in order to recover the normal shape and position of pupil, improve postoperative visual acuity and prevent monodiplopia and glare. METHODS: In the cases which have iridosteresis at the direction of 9-3 oclock or updrawn pupil, iris suture was performed at the incision directly(open method); while those at the direction of 3-9 o'clock, operation was performed outside the anterior chamber (close method). RESULTS: In 22 cases, the percentage which having visual acuity better than 0.5 is 65%, having nearly round shape pupil 67%, having pupil diameter less than 3 mm 73%, having normal pupil position 67%. CONCLUSION: All kinds of deformed pupil can be recovered to their normal shape, size and position by performing corepraxia while IOL was implanted. The prolene has light reaction and better effect in iris suture.

Adolescent↗
PubMed2001

[Conjugation of mitoxantrone-loaded nanospheres and anti-C-erbB-2 monoclonal antibodies].

AIM: To improve the treatment efficacy of anti-tumor drug mitoxantrone, the conjugation of mitoxantrone-loaded nanospheres and anti-C-erbB-2 monoclonal antibodies were prepared. METHODS: Mitoxantrone-loaded nanospheres were prepared with emulsion-heating solidification technique. A heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP), was used as the crosslinker of mitoxantrone-loaded nanospheres and anti-C-erbB-2 monoclonal antibodies; pharmaceutical properties of immunonanocapsuls were studied; the conjugates of nanospheres and monoclonal antibodies was confirmed with immunological methods such as slide agglutination test, fluorescent immunossay and rosset formation test, fluorescent staining and scanning electron microscope. RESULTS: Mitoxantrone-loaded nanospheres were spherical, with smooth surface and median diameter of 0.665 micron. When stored at 3-5, 20-25 and 37 degrees C, RH 75% for three months, the appearance, morphology, size distribution, drug loading and in vitro release characteristics showed no significant change and the stability was satisfactory. The size analysis demonstrated that there was no obvious increase in the particle size of nanoparticles after conjugation. Immunological tests indicate highly selective binding of antibody-targeted nanospheres to C-erbB-2-overexpressing cells SK-BR-3. CONCLUSION: The conjugation of mitoxantrone-loaded nanospheres and anti-C-erbB-2 monoclonal antibodies can keep the activity of anti-C-erbB-2 and increase the therapeutic efficacy of anti-mammary cancer drugs.

Antibodies, Monoclonal↗
PubMed2001

[An integrated medical diagnostic X-ray unit controlled by microcomputer].

This article introduces an integrated medical diagnostic X-ray unit controlled by microcomputer. It is a practical and low-priced spot-film radiography device, and it is applicable to conventional local control unit. If you select a set of X-rayimage intensification, it can be remotely controlled. This device utilizes DC servo motor and fine precision feedback variable resistor and closed loop control so it has the advantage of fine static control and low mechanical noise. The whole radiographic process is automatically controlled by microcomputer, and can be operated easily.

Equipment Design↗
PubMed2001

[Investigation of the bioadhesion of carbopol and hydroxypropyl methylcellulose to rat gastrointestinal mucosa in vivo and in vitro].

AIM: To investigate bioadhesion of carbopol and hydroxypropyl methylcellulose of different viscosity grade to rat gastrointestinal mucosa. METHODS: The maximum adhesion force has been adopted as an index of adhesive evaluation in vitro, gastro-emptying rate and migrating length in intestine have been adopted as an index of adhesive evaluation in vivo. RESULTS: In vitro adhesion studies indicated that the adhesive force of low-vicosity materials was stronger than that of high-vicosity materials, while the transferation rate of high-vicosity materials is significantly higher compared to that of low-vicosity materials in vivo. CONCLUSION: Adhesive force of materials gives no correlation with rate of the materials in rat; Cb934 have the optium bioadhesion among the studied materials and can be used as the preferred adjuvant in oral bioadhesion preparation.

Acrylic Resins↗
PubMed2001

[Biochemical remodeling of myocardial collagen and its relation to cardiac renin-angiotensin system in rats after myocardial infarction].

OBJECTIVE: This study aimed at the biochemical remodeling of myocardial collagen and its relation to cardiac renin-angiotensin system (RAS) after myocardial infarction(MI). METHODS: The ventricular and plasma angiotensin II (Ang II) content, total collagen content, and collagen concentration in left ventricle(LV) and right ventricle(RV) of MI and sham-operated control(SOC) rats on 3, 15 and 42 days after operation were measured dynamically by means of radioimmunoassay etc. RESULTS: The plasma Ang II content increased only in MI rats on day 3. The Ang II content in LV of the MI group increased markedly on day 3 after MI as compared with that of the SOC group. It sustained at this higher level on the 15th and 42th days, and the similar changes were observed in RV as well. The biochemical remodeling of collagen in LV and RV occurred after MI and the remodeling of collagen showed similar patterns with its Ang II content, except that collagen remodeling in RV was delayed than the change of Ang II content. CONCLUSION: The cardiac RAS was obviously activated after MI. Ventricular Ang II might play an important role in the biochemical remodeling of myocardial collagen, and its role was independent of circulating RAS.

Angiotensin II↗
PubMed2001

[Determination of warfarin in plasma by HPLC and an investigation of monitoring patients after cardiac valve replacement].

OBJECTIVE: A simple HPLC method was established for the determination of warfarin in plasma to investigate the relationship between warfarin concentration and anticoagulant effect. METHODS: The mixture of dichloromethane and hexane (1:9) was used as extracting solvent for the plasma samples. The chromatographic separation was on C18 column with a mobile phase consisting of methanol and 50 mmol/L ammonium acetate buffer (pH2.5, 70:30). RESULTS: The calibration curve was linear within 50-2000 ng/ml. The extraction recoveries of warfarin were 78.0%-81.6%. The recoveries of methodology were 103.1%-106.5%. Inter-day and intra-day RSD were 2.33%-5.46% and 5.29%-7.73%, respectively. This method was used for determining warfarin in 70 patients after cardiac valve replacement. The results showed that 44 cases had their INR within the safety range (1.31-2.35) recommended to Chinese, and of them 37(84.1%) cases had a warfarin level at 616.2 +/- 154.8 ng/ml. CONCLUSION: This method is useful in monitoring warfarin concentration during anticoagulant therapy.

Anticoagulants↗