Biomedical subjects

Y Huang

Publications and source records attributed to Y Huang.

At least 253 records · Page 14Linked to original sources

PubMed2001

Excessive Cu and Zn affecting on distribution of the metals and activities of glycolytic and nitrogen incorporating key enzymes in mycelia of ectomycorrhizal fungi Suillus bovinus.

Concentration of copper and zinc in isolated Suillus bovinus mycelia, used nutrient solution and 0.5 mol/L EDTA mycelia washing solution were measured to investigate the distribution of heavy metals in mycelia growth in excess copper or zinc nutrient solution. Treated with zinc, most of added zinc maintained in used solution, and 9.8%/14.6% was in/on mycelia in treatment, and in treatment 2 was 3.9%/8.0% in/on mycelia. In the copper applications, copper stimulated in more than on mycelia, i.e., 25.9%/4.5% in/on mycelia in treatment, and 7%/18.8% in/on mycelia while most of copper retained in used nutrient solution. Certain amount of copper or zinc uptake by mycelia led to pronounced influence on glycolysis and nitrogen incorporating process of Suillus bovinus, while the tested enzymes kept constant in treatment. In crude extracts of copper treatment 2 mycelia, activities of HK, PFK and GS were inhibited and decrease to 63%, 48% and 38% and GIDH increased by 68% of the control, respectively. The behaviors of these tested enzymes toward zinc corresponded in general with that towards copper. The potential protection of Suillus bovinus for its host plant under excess copper or zinc threaten was discussed.

Basidiomycota↗

PubMed2001

[Establishment of cord blood stem cell bank and its clinical application].

OBJECTIVE: To establish unrelated cord blood stem cell bank for providing potential grafts for stem cell transplantation in the treatment of some malignant and nonmalignant disorders. METHODS: All cord blood units(CBU) were obtained from full term normal vaginal and Caesarean-section deliveries. Most of RBC was removed from CBU by the hydroxyethyl starch (HES) method. CBUs were cryopreserved in liquid nitrogen using DMSO and LMD as cryoprotectants. For each CBU, automated cell count, manual viability, HLA typing, screening for infectious agents, colony-forming assays and CD34 analysis by FACS were routinely performed. RESULTS: A total of 2,318 CBUs were collected, among which, 1,240 CBUs met all tests and standards. The average volume of collected qualified cord blood was (98.31 +/- 26.95) ml, the average amount of nucleated cells after separation was (1.22 +/- 0.47) x 10(9), and the average recovery percentage of nucleated cells (88.90 +/- 9.65)%. The average viability was (97 +/- 2.61)%, the number of CFU-GM (5.18 +/- 14.35) x 10(5), of BFU-E (10.35 +/- 11.55) x 10(5), and of CFU-GEMM (1.03 +/- 1.47) x 10(5). The average percentage of CD34+ cells after CBU separation was (0.5 +/- 0.32)%. Four units were positive in microbiological culture and 4 units CMV-IgM positive. In 172 patients registeredd for cord blood transplantation in Guangzhou Cord Blood Bank, the percentage of 4/6 HLA antigens matched units was 91.12%. Up to May 2000, 9 units had been used in unrelated cord blood stem cell transplantation and 6 of them were successfully engrafted. CONCLUSION: It suggested that cord blood bank could be useful in transplantation and the establishment of cord blood bank had a good prospect.

Blood Banks↗

PubMed2001

[Expression of cytokines in acute heart transplantation rejection].

OBJECTIVE: To observe the expression and changes of local cytokines network in rat heart transplantation so as to determine the role of cytokines in acute rejection of rat heart transplantation. METHODS: The Wistar rats (receptor) were divided into four groups: group A(control, n = 12), group B(IL-2 Mab treated, n = 12), group C [Cyclosporine A (CsA) treated, n = 12] and group D(IL-2Mab + CsA treated, n = 12). Hearts from SD rats were transplanted into a cervical location in Wistar. The local expression of IL-1 beta, IL-2, CD25, IL-4, IL-5, IL-6, IL-10, TNF alpha, IFN gamma was examined at 1, 3, 5, 7, 9, 11, 14 days after operation by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Allografts survival time was (8.3 +/- 1.7) days in group A, (29.2 +/- 7.1) days in group B, (26.4 +/- 5.7) days in group C, (55.0 +/- 10.6) days in group D. The expression of IL-1 beta, IL-4, IL-10, IFN gamma was up-regulated, and the expression of IL-2, CD25, IL-5, IL-6, TNF alpha was significantly inhibited in group A. The expression of IL-1 beta, IL-5, IL-6, IL-10, IFN gamma was up-regulated, and the expression of IL-2, IL-4, TNF alpha was significantly down-regulated in group B. The expression of IL-1 beta, IL-2, CD25, IL-5, TNF alpha, IFN gamma was up-regulated, and the expression of IL-4, IL-6, Il-10 was significantly down-regulated in group C. The expression of IL-4, IL-5, IL-6, IL-10 was up-regulated, and the expression of IL-1 beta, IL-2, CD25, IFN gamma, TNF alpha was significantly down-regulated in group D. CONCLUSIONS: Cytokines play an important role in the development of acute transplantational rejection. Different cytokines play different roles in different local environments; the main principle possibly is immune deviation.

Acute Disease↗

PubMed2001

[Pretreatment of benzoic acid wastewater].

The benzoic acid wastewater produced from toluene by liquid-phase oxidation is one of the difficult wastewater to treat because of multi-component, high CODcr value (20,000-100,000 mg/L) and nonbiodegradability. A pretreatment way of this wastewater by extraction based on chemical complexation was carried out, and tributyl phosphate (TBP), trialkylamine (7301) and trialkyphosphine oxide (TRPO) were used as complexing agent with n-octanol, kerosene as cosolvent and diluent respectively. Results showed that benzoic acid was recovery successfully and the CODcr value of wastewater was reduced to 1/6 of the initial value through multi-stages cross-flow extraction. Back-extraction was quite easy and the solvent could be reused.

Benzoic Acid↗

PubMed2001

[A clinical study on the effects of delayed rapid fluid resuscitation on the blood circulation during postburn shock stage].

OBJECTIVE: To explore the protocol for the quick correction of postburn shock in case of delayed resuscitation. METHODS: Twenty burn patients inflicted with 40% or bigger TBSA burn, and who were in shock due to delayed admission to hospital, were enrolled in the study. The patients were treated by delayed rapid fluid resuscitation. The amount of infused fluid and urine output was observed. The indices of hemodynamics and oxygen metabolism, i.e. arterial blood pressure (BP), pulmonary arterial pressure (PAP), pulmonary arterial wedge pressure (PAWP), central venous pressure(CVP), cardiac output (CO), pulmonary vascular resistance (PVR), systemic vascular resistance (SVR), oxygen delivery (DO2), oxygen consumption (VO2), oxygen extraction (O2ext), lactic acid (LA) and base deficit (BD) were monitored at the shock stage(1 approximately 48 PBHs). RESULTS: The amount of rapid fluid infusion within 2 hours after admission accounted for (38.8 +/- 6.1)% of the amount calculated with the formula (The Third Military Medical University burn shock fluid infusion formula) for the 1st 24 PBHs. When the amount of prehospital infusion was added, the amount would be (48.3 +/- 5.0)% of the amount for the 1st 24 PBHs. The real amount of the infusion for the 1st 24 PBHs was (31.4 +/- 14.3)% more than that of the formula amount, and the real infused fluid amount for the 2nd 24 PBHs was (3.2 +/- 7.2)% more than that of the formula amount. After rapid fluid infusion, there exhibited remarkable increase in urine output, CO and DO2 with evident decrease in SVR, LA and BD. Furthermore, PAWP, PAP and CVP remained within normal range even though PVR increased significantly after rapid fluid infusion. CONCLUSION: In case of shock or compulsory delayed resuscitation, rapid fluid resuscitation during early postburn stage was beneficial with critical hemodynamic monitoring. The amount of delayed rapid fluid infusion was much increased than routine. Hemodynamic indices such as CO, PAP, PAWP and CVP were employed as the guidelines for delayed rapid resuscitation with reference to some clinical indices such as serum LA, blood gas analysis and urine output.

Adolescent↗

PubMed2001

[Study on the relation between the angiotensin converting enzyme gene and pregnancy induced hypertension].

OBJECTIVE: To study the relationship between the 16th intron insertion/deletion (I/D) polymorphism of angiotensin converting enzyme (ACE) gene and pregnancy induced hypertension (PIH). METHODS: The 16th intron I/D polymorphism of ACE gene in 60 PIH women (PIH group) and 50 normal pregnant women (control group) was detected by PCR. The type of ACE gene in PIH group was assessed and the serum concentration of angiotensin II (AngII) was compared between PIH group and control group. RESULTS: ACE gene types in the PIH women included: insertion homozygote II, deletion homozygote (DD) and insertion/deletion heterozygote (ID). The gene frequency was 55.0% (33/60) for II, 26.7% (16/60) for ID and 18.3% (11/60) for DD respectively. The frequency of insertion allelotype (0.7) of PIH group was higher than that of control group (0.3) (P < 0.05). The Ang II concentration in DD type PIH women was higher than that in control group although the Ang II concentration in II type and I D type PIH was not different from control. CONCLUSIONS: ACE gene polymorphism in the PIH women included: insertion homozygote II, deletion homozygote (DD) and insertion/deletion heterozygote (ID). The concentration of Ang II in DD type PIH women was higher than in control. It is suggested that the pathogenesis of PIH may be connected with multiple genes and factors.

Adult↗

PubMed2001

Alterations in myosin heavy chain isoform gene expression during the transition from compensatory hypertrophy to congestive heart failure in rats.

OBJECTIVE: To explore the molecular mechanism underlying the decreased velocity of tension rise in rat myocardium during congestive heart failure (CHF) and left ventricular hypertrophy (LVH) induced by aortic stenosis. METHODS: The maximum velocity of tension rise (+dT/dtmax) was measured in left ventricular papillary muscle and the mRNA level of myosin heavy chain (MHC) isoforms in the left ventricle were detected by Northern blot analysis. RESULTS: The value of +dT/dtmax in CHF and LVH group were 64.17% and 37.15% lower than sham-operated controls (Sham) (P < 0.01); values in the CHF group were 42.99% lower than that of LVH (P < 0.01). The level of alpha-MHC mRNA in LVH was not different from that of the Sham (P > 0.05), but decreased significantly in CHF to 42.3% of Sham and 56.1% of LVH (P < 0.01). The level of beta-MHC mRNA was up-regulated by 88.3% (P < 0.01) in LVH compared with Sham and the level of beta-MHC in CHF was 1.5-fold and 3.7-fold higher than that in LVH and Sham respectively (P < 0.01). The ratio of alpha-MHC/beta-MHC mRNA in LVH and CHF decreased to 42.4% and 9.8% respectively of the value in Sham (P < 0.01). Correlation between alpha-MHC/beta-MHC mRNA level and +dT/dtmax was analyzed which showed that these values were positively correlated with a correlation coefficient of 0.875 (P < 0.01). CONCLUSION: The decreased ratio of alpha-MHC/beta-MHC mRNA was the major molecular mechanism underlying the decreased +dT/dtmax in CHF and LVH myocardium. The decreased ratio of alpha-MHC/beta-MHC mRNA in LVH was mainly due to the up regulation of beta-MHC mRNA while in CHF both down regulation of alpha-MHC and up regulation of beta-MHC were involved.

Analysis of Variance↗

PubMed2001

Relationship between gene polymorphism of the PAI-1 promoter and myocardial infarction.

OBJECTIVE: To investigate the association between gene polymorphism of the plasminogen activator inhibitor-1 (PAI-1) and myocardial infarction (MI) in Chinese. METHODS: PAI-1 genotyping with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and allele specific polymerase chain reaction (ASPCR) was performed in 87 myocardial infarction patients and 92 unrelated healthy controls. All subjects' clinical features and PAI-1 activity were tested. RESULTS: There were two polymorphisms within the promoter, a G/A single base substitution polymorphism upstream at -844 bp, and a single guanosine deletion/insertion 4G/5G polymorphism -675 bp upstream from the start of transcription. Significant differences between the patients and the controls were observed neither for the frequencies of the GG, GA and AA genotypes nor for the PAI-1 activities of these three types. But for the 4G/5G polymorphism, there were significant differences between patients and controls for the frequencies of the 4G/4G, 4G/5G and 5G/5G genotypes (P < 0.05). In the MI group, the PAI-1 activity of the 4G/4G type was significantly higher than that of the 5G/5G type (P < 0.05). Further more, a positive correlation between the glucose level and PAI-1 activity was found (r = 0.34, P = 0.02). CONCLUSION: This study indicates that the 4G/5G gene polymorphism of PAI-1 is associated with myocardial infarction, that 4G/4G type is probably an important hereditary risk factor, and that glucose has functional importance in regulating PAI-1 activity.

Adult↗

PubMed2001

Clinical and familial study of arrhythmogenic right ventricular cardiomyopathy.

OBJECTIVE: To explore the characteristics of arrhythmogenic right ventricular cardiomyopathy (ARVC). METHODS: Seven patients with arrhythmogenic right ventricular cardiomyopathy and 34 members of three families were studied. All patients and family members underwent history collection, clinical examination, electrocardiogram (ECG), two-dimensional echocardiography (2-DE) and a signal averaging electrocardiogram. Programmed ventricular stimulation was performed in five patients. RESULTS: All patients and family members had normal morphologic characteristics and normal function of the left ventricular by 2-DE. Fourteen persons had abnormal findings indicating ARVC. Five had enlargement of the right ventricular with diffused hypocontractility, eight had thin and systolic bulging in the focal anterior wall with hypokinesia and one had bulging of the inferior wall. Twenty-five persons (seven patients and 18 family members) had abnormal findings in ECG. Positive ventricular late potential was recorded in 13 persons (six patients). Two to three monomorphic ventricular tachycardia (VT) with left bundle branch block (LBBB) configurations were induced in five patients. Ventricular fibrillation was induced in two patients during the electrophysiologic study (EPS). Five patients had very high pacing threshold and/or ineffective pacing in one or many regions of the right ventricle. Two members of one family died suddenly. One member was a dwarf with ARVC. Spontaneous VT with a left bundle branch block (LBBB) configuration was recorded in five patients, polymorphic VT with extremely short coupling interval in one, and premature ventricular complexes with LBBB configuration in 12 (six patients). CONCLUSION: Our familial study strongly suggests that ARVC may be a hereditary disease and it is helpful in the diagnosis and detection of ARVC. The most common manifestations were abnormal structure and function of the right ventricle and abnormal ECG of repolarization and ventricular arrhythmia which originates from the right ventricle.

Adolescent↗

PubMed2001

Novel chromosomal alterations detected in primary nasopharyngeal carcinoma by comparative genomic hybridization.

OBJECTIVE: To gain a better understanding of genetic changes in Cantonese nasopharyngeal carcinoma (NPC). METHODS: Comparative genomic hybridization (CGH) was performed on 17 primary nasopharyngeal carcinomas. RESULTS: A novel copy number gain an chromosome 4q and loss of chromosome 1p were found at a high frequency (> 50%). CONCLUSIONS: Current analysis revealed a comprehensive profile of the chromosomal regions showing gain of chromosomes 4q, 12q, and 1q as well as loss of chromosomes 1p, 3p, 11q, 14q, 15q, 13q, Xq, 9q, 10p, 10q, and 16q. Frequently altered loci may encode oncogenes or tumor suppressor genes involved in the development of primary NPC.

Chromosome Aberrations↗

PubMed2001

[Treatment of stage II non-Hodgkin's lymphoma: analysis of 268 patients].

OBJECTIVE: To seek the optimum treatment for patients with stage II non-Hodgkin's Lymphoma (NHL). METHODS: 268 patients with stage II NHL, treated from January 1987 to December 1993, received radiation alone (R-50), radiation plus chemotherapy (R + C - 73), chemotherapy followed by radiation then by chemotherapy (C + R + C - 72), chemotherapy plus radiation (C + R - 61) or chemotherapy alone (C-12). A total of 206 patients was treated with combined treatment. RESULTS: The 1-, 2-, 3-, 4-, 5- and 6-year survival rates were 95.1%, 87.8%, 87.8%, 85.4%, 82.9%, 53.6% in C + R + C group for patients with stage II high grade NHL, which were much better than those in the R group, R + C group and C + R group (P < 0.01). For patients with stage II intermediate grade NHL, the 1-, 2-, 3-, 4-, 5- and 6-year survival rates were 89.3%, 75.0%, 67.8%, 60.6%, 57.1%, 46.4% in the C + R + C group, which were better than those in the R group, R + C group and C + R group (P > 0.05). However, for patients with low grade NHL, the survival rates were similar both in the R group and the combination groups. CONCLUSION: For patients with stage II intermediate and high grade NHL, combination therapy of C + R + C is recommended. Radiation alone can only be used for patients with stage II low grade NHL.

Adolescent↗

PubMed2001

[Incidence of pregnancy induced hypertension and the effects on mother and fetus in Shanghai during 1989-1998].

OBJECTIVE: To investigate the incidence of pregnancy induced hypertension (PIH) in Shanghai during 1989-1998. METHODS: The data were analyzed retrospectively from three municipal general hospitals and five special hospitals obstetrics and gynecology. RESULTS: There were 158,790 deliveries in ten years. The total numbers of PIH were 8,852 cases. The incidence of PIH was 5.57%. The rate of mild, moderate and severe PIH was 55.83%, 29.39%, and 14.78% respectively. There were 114 cases (1.29%) complicated with eclampsia. The rate of cesarean section was 66.97% in PIH patients. The complications of PIH were as following: abruptio of Placenta 0.52%, heart failure 0.34%, renal failure 0.20%, DIC 0.07% hemolysis, elevated liver enzymes and low platelet count(HELLP) syndrome 0.03%, maternal death one case. There were 9,197 birth of 8,852 cases of PIH (twin 343 cases, triplets one cases). The perinatal death 66 occurred in cases (7.18@1000) in which fetal death 48 cases, stillbirth 12 and neonatal death 6. CONCLUSION: The incidence of PIH and the rate of perinatal infant death can be reduced by strengthen antenatal monitoring, prevention, early diagnosis and treatment of PIH.

China↗

PubMed2001

[Studies on the cell suspension culture of Saussarea medusa in a stirred tank bioreactor].

The cell suspension culture of Saussarea medusa in a 2L aerated and agitated bioreactor with a four-pitch-blade impeller was investigated. The effects of agitation speed, aeration and inoculum size on cell growth and flavonoids production were studied and it was found that cells had optimum growth and flavonoids production when cultivated at 75 r/min, 700-1000 L/min and an inoculum of 4.0-5.0 g/L. A high cell biomass of 13.8 g/L and flavonoids production of 416 mg/L were achieved after 12 days of cultivation. Time course study revealed that flavonoids biosynthesis was growth-associated. The studies on aggregates size distribution in the bioreactor showed that the aggregates break-up caused by hydrodynamic stress might adversely affect cell growth and lead to significant reduction of cell biomass and flavonoids production.

Bioreactors↗

PubMed2001

[Values of TNF, IL-8, sICAM-1 and CD11b/CD18 in benign and malignant pleural effusions].

OBJECTIVES: To explore the significance of TNF, IL-8, sICAM-1 and CD11b/CD18 in pleural effusions and/or in peripheral blood in formation of pleural effusions and the possible role in differential diagnosis. METHODS: Levels of TNF, IL-8, sICAM-1 and PMN CD11b/CD18 expression were measured by radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA) and flow cytometry (FCM). 31 patients with tuberculous pleural effusions, 31 malignant pleural effusions and 31 healthy persons as control were studied. RESULTS: The serum levels of TNF, IL-8 and PMN CD11b/CD18 expression in benign and malignant effusions were markedly higher than those in controls (P < 0.01). The levels of TNF, IL-8 and PMN CD11b/CD18 in patients with tuberculous pleural effusions were elevated higher than those with malignant effusions (P < 0.01). The levels of sICAM-1 in pleural effusions were much lower in tuberculosis than malignancy (P < 0.01). TNF levels in pleural effusions were positively correlated to the IL-8 and sICAM-1 levels (r = 0.74 and 0.79, respectively, P < 0.01). TNF levels in serum was positively correlated to the PMN CD11b/CD18 expression (r = 0.61, P < 0.01), but the latter was negatively correlated to sICAM-1 levels of pleural effusions (Y = 1442.31 - 36.85X + 0.25X(2), R(2) = 0.59, F = 19.83, P < 0.01). CONCLUSIONS: The results show that TNF, IL-8, sICAM-1 and CD11b/CD18 may work and affect each other in immunopathological process of tuberculous and malignant pleural effusions. The changes of TNF, IL-8 in pleural effusions and the expressions of PMN CD11b/CD18 are of clinically diagnostic value in distinguishing tuberculous from malignant pleural effusions.

Adult↗

PubMed2001

[A study on steam generator in solid amine CO2 purification system].

Objective. To solve the key problems of power matching between process of CO2 steam desorption and process of steam generation, as well as water/vapor separation. Method. Solid amine desorption process was studied by thermodynamic analysis and experiments. The distribution rule of desorption energy was found out and then the power consumption of steam generator was decided. Ceramic insert was designed to separate water and vapor making use of surface tension. Finally, the steam generator was designed on system requirements. Result. Experiments proved that the steam generator can satisfy the demand of the system as well as successfully separate water and vapor, in addition, the selected power is suitable. Conclusion. The design on steam generator was right and practicable.

Air Conditioning↗

PubMed2001

Wolfram syndrome in a family with variable expression.

Wolfram syndrome is a rare neurodegenerative disorder with autosomal recessive inheritance. The main characteristic features of this disorder are diabetes mellitus and optic atrophy. However, diabetes insipidus, sensorineural deafness, renal tract and neurologic abnormalities are seen in majority of patients. In this study, we describe a family in which two members had the main features of the syndrome while a third sibling had only sensorineural deafness. DNA analysis revealed that the fully affected siblings were homozygote for a pointmutation on chromosome 4p whereas the third sibling with deafness was a heterozygote carrier for the same mutation. The characteristics of disease and phenotypic variations that possibly related to heterozygote carrier state were discussed.

Adolescent↗

PubMed2001

[Influence of planting density and precipitation on N2O emission from a winter wheat field].

To investigate the impact of plant density on N2O emission from winter wheat field and the cause of seasonal variation in the emission, field experiment with four planting rates of 0, 90, 180 and 270 kg/ha was conducted at the Jiangning County near Nanjing during 1999-2000 winter wheat growing season. Data of the field measurements indicated that the N2O emission rates during the season from planting to overwintering were not influenced by the plant density, while the emission was positively correlated with the planting density during the season from turning green to maturity. The emissions from the field plots with planting rates of 0 and 90 kg/ha were not found to be significantly different. A further analysis suggested that the seasonal variation of N2O emission be mainly influenced by precipitation, which could be quantitatively described by an exponential function of a weighted average precipitation of 6-day period before measurement.

Agriculture↗

PubMed2001

[Effects of grazing and cultivating on emission of nitrous oxide, carbon dioxide and uptake of methane from grasslands].

Measurement of N2O, CO2 and CH4 with the optimized closed chamber/GC technique in the typical grassland, which were influenced by human activities, grazing and cultivating first time in situ were carried out in Inner Mongolia of P. R. China from 1998 to 1999. It was found that wheat cropping can increase N2O production 3 times than the native, and changed a CO2 sink of Inner Mongolia Steppe to a significant source, increased efflux CO2(C) average 14.3 mg.(m2.h)-1 from Jun. to Dec. in 1998. CH4 uptake was highly increased if there was grazing lightly. There were no effects of wheat cropping on CH4 uptake and no significant results of grazing on N2O emission. Soil moisture and temperature were the key factors, other than human activities, which affect the patterns of seasonal variation of N2O and CO2 emission and CH4 uptake. Highly inter-annual variations, which resulted from the annual precipitation, of the exchange fluxes and seasonal variation patterns of N2O and CO2 emission and CH4 uptake there were. It was a fact that N2O and CO2 emission from the soils was inversely related to CH4 uptake.

Animal Husbandry↗