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Biomedical subjects

Y Horiuchi

Publications and source records attributed to Y Horiuchi.

At least 163 records · Page 9Linked to original sources
PubMed1985

Leser-Trélat sign associated with Sézary syndrome.

The Leser-Trélat sign is a rare but well known cutaneous indicator of internal malignancy, most commonly adenocarcinoma of the stomach. There have been only a few cases associated with lymphoproliferative malignancies. Sézary syndrome is a chronic leukemia/lymphoma characterized by generalized erythroderma and circulating Sézary cells. We describe a rare case of the Leser-Trélat sign associated with the Sézary syndrome. The skin sign was alleviated through combination chemotherapy.

Aged↗
PubMed1984

A multicenter double-blind controlled study of lobenzarit, a novel immunomodulator, in rheumatoid arthritis.

A multicenter double-blind study was carried out in patients with rheumatoid arthritis (RA) by comparing treatment with a novel immunomodulator, lobenzarit, disodium 4-chloro-2, 2'-iminodibenzoate, with an inert placebo. Both groups of patients received 75 mg/day of indomethacin as a basal regimen during the study period of 16 weeks. Group 1 (115 patients) received 240 mg/day lobenzarit, 80 mg TID, and Group 2 (115 patients) received placebo TID orally. A statistically significant improvement was noted in the number of swollen joints and in the Lansbury index at Weeks 12 and 16 in Group 1 as compared to Group 2. Overall clinical effectiveness was significantly higher in Group 1 (63%) than in Group 2 (43%). Incidence of side effects was 38% in Group 1 and 22% in Group 2. The most frequent side effect in both groups was gastrointestinal upset. Our data confirm that lobenzarit is a useful agent in the treatment of patients with RA.

Arthritis, Rheumatoid↗
PubMed1983

Heterogeneity of antilymphocyte antibody in SLE and its correlation with disease activity.

The cytotoxic activities of individual lupus sera were demonstrated against T gamma cells, T gamma (-) cells and B cells. Immunoglobulin classes were also determined in these antilymphocyte antibodies (ALA) in SLE. T gamma-specific ALA and B-cell-specific ALA were almost equally distributed as regards IgG-dominant type, mixed IgG and IgM type and IgM dominant type, while IgM dominant type was predominant in T gamma (-)-specific ALA. Only the IgG type of T gamma-specific ALA among these ALA was significantly associated with clinical parameters, including hypocomplementemia, elevated immune complex levels and high anti-double-stranded (ds) DNA titres. Serial studies on ALA in a typical case of SLE were performed. Active clinical signs were associated with elevated cytotoxicities of IgG type of T gamma-specific ALA and with relatively low cytotoxicities of the IgM type, whereas after massive corticosteroid therapy, these signs disappeared in tact with the reduction in cytotoxic activities of IgG type of the ALA and the relative rise in cytotoxicities of IgM type of the ALA. These results suggested that IgG class of T gamma-specific ALA seemed to play a major role among several types of ALA in SLE.

Antibodies, Antinuclear↗
PubMed1983

[Experimental study on peripheral nerve lesions--compression neuropathy].

Compression is one of the most important causes of entrapment neuropathy. The experimental study described was performed to elucidate the pathogenesis of compression neuropathy in the sciatic nerves of 113 mongrel dogs. An apparatus, devised to compress the nerve constantly with the force of 15, 30 and 50 grams (27.6, 55.2, 92.0 mmHg), was applied to the sciatic nerve 4 cm distal to the sciatic tuberculum. The following observations were made on the 1st, 2nd, 3rd, 4th, 6th and 8th weeks. Macroscopic findings: The compressed site of the nerve was thinned and flattened. Adjacent portions both proximal and distal to the compressed site showed distinct swelling (prox. greater than dist.). Electrophysiological findings: In the 15-gram-compression group, the conductability was maintained up to the 8th week, but the motor nerve conduction velocity was reduced to approximately 60% of the control. In the 30-gram-compression group, the conductability of the compressed site was lost within one week. However, the conductability in the distal portion remained detectable. In the 50-gram-compression group, the conductability was completely lost within one week. Histological findings (H and E, Holmes and LFB stains): In the pseudoneuroma, the swelling of the individual axons was slight, but the arrangement of each axon and each funiculus was extremely sparse because of the exudate in the interstitial tissue. At the compressed site, axons were closely packed together with a concomitant decrease of interstitial tissue. In the 15-gram-compression group. Wallerian degeneration was localized in large myelinated fibers at the distal site. In the 30-gram-compression group, almost all the nerve fibers except for small ones showed degeneration. In the 50-gram-compression group, all fibers degenerated. Teased single fiber specimens: In the 15-gram-compression group, paranodal demyelination was seen from the 1st week. Up to the 8th week, such demyelination was more remarkable, and a bulbous deformity appeared at the proximal site of compression and Wallerian degeneration of the large myelinated fibers occurred at the distal site of compression. Microangiogram (India ink method): The internal microvessels decreased slightly in the 15-gram-compression group.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1982

Association between HLA and Japanese patients with rheumatoid arthritis.

Japanese patients with rheumatoid arthritis (RA) were observed to have a statistical association with HLA-DR4, MT3. Strong association between the clinical severity of RA and HLA was also observed. Male patients had a stronger association with HLA than female patients. Males are more resistant to RA than females. This suggested that the threshold of liability for RA is higher in males than in females. Japanese patients with RA with systemic vasculitis were negative for HLA-Bw44 and had antilymphocytotoxic autoantibody, indicating that RA with systemic vasculitis is different in etiology from RA without systemic vasculitis.

Adult↗
PubMed1982

Reduced natural killer cell activity of lymphocytes from patients with Werner's syndrome and recovery of its activity by purified human leucocyte interferon.

Natural killing (NK) activities were measured in peripheral blood lymphocytes (PBL) from 14 patients with Werner's syndrome (WS), and the results were compared with those of 187 normal individuals at different ages. The NK activities of healthy donors were demonstrated a characteristic and invariable manner in accordance with the age and the sex, whereas the activities of WS patients were considerably reduced irrespective of the age and the sex. When normal PBL were preincubated with WS sera containing anti-lymphocyte antibodies (ALA) plus rabbit complement, the NK activities of the surviving PBL were markedly reduced when compared with those of PBL treated with other heterologous ALA of rabbit origin in the same manner. PBL from WS patients treated with purified human leucocyte interferon augmented the NK activity even beyond the range of controls. These results suggested that NK activities in WS patients were reduced in a manner similar to that in normal old individuals. The ALA reactive to NK cells in WS patients may participate in the reduction of NK activities. The augmentative effect of interferon on NK activities in WS patients may also suggest the possible blocking of potent NK activities by an unknown mechanism rather than an intrinsic defect of their NK cells.

Adolescent↗
PubMed1982

Isolation of human suppressor T cells by peanut agglutinin.

Peanut agglutinin (PNA) is a plant lectin which has been used for the separation of suppressor T cells in mice. The present study was carried out to determine whether PNA would be useful in the isolation of human suppressor T cells. Normal human peripheral blood mononuclear cells (PBMC) were activated by concanavalin A (Con A) and the resultant blastoid cells were then incubated with PNA-coated autologous erythrocytes to induce the rosette formation. The PNA rosette-forming cells (15-18% of Con A-activated PBMC) were separated from non-rosette-forming cells by differential centrifugation. The rosette-forming cells suppressed pokeweed mitogen-induced polyclonal immunoglobulin synthesis by 2- to 5-fold. This degree of suppression was greater than that seen with non-rosette-forming cells or with unseparated Con A-activated cells. Our data indicate that PNA rosette fractionation provides a simple and useful method for the isolation of human suppressor T cells.

Cell Separation↗
PubMed1982

T lymphocyte interaction with immunoglobulin G antibody in systemic lupus erythematosus.

Systemic lupus erythematosus (SLE) is an autoimmune disease with multiple immune disturbances whose mechanisms remain unclear. We examined the interaction of antilymphocyte antibodies with cultured normal T lymphocytes. T cells were prepared by E-rosetting after petri-dish removal of adherent cells and cultured for 2-7 d in the presence of SLE sera or normal human sera. Cultured T cells were washed and sonicated, and the amount of cell-associated IgG was quantitated by radioimmunoassay or enzyme-linked immunoassay (ELISA) methods. T cells cultured with 27 of 39 SLE sera showed marked increments of associated immunoglobulin G (IgG) although this was not observed with sera from mixed connective tissue disease patients containing high titers of ribonucleoprotein antibody or normal donors. The effective factors for IgG association in SLE sera were absorbed with normal peripheral blood lymphocytes or T cells. Anti-T cell IgG cytotoxic activity strongly correlated with T cell IgG association (P less than 0.01). T cell-associated IgG was not removed by stripping of cell membrane IgG from living cells by acid buffer treatment; indirect immunofluorescence of cells fixed after 2-4 d of culture revealed cytoplasmic IgG staining. IgG anti-T cell antibodies appeared to associate inside the cell membrane or to penetrate into the cytoplasm of cells. T cell Fc receptor blocking by heat-aggregated IgG or anti-beta 2-microglobulin antibody did not alter IgG cell association. Since pepsin-digested SLE sera showed no T cell association activity, whole IgG antibody molecules appeared to be necessary for interaction with cultured T cells. In addition, reduction and alkylation of active SLE sera completely nullified T cell reactivity. When normal T cells were cultured with SLE sera showing marked IgG T cell association, viability of cultured T cells decreased rapidly after 4 d, which suggests that IgG anti-T cell antibodies were associated with cell destruction. IgG cell-associating antilymphocyte antibodies present in SLE sera may cause T cell disturbances in vivo and may be related to the lymphocytopenia present in SLE patients.

Adult↗