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Biomedical subjects

Y Hamada

Publications and source records attributed to Y Hamada.

At least 487 records · Page 27Linked to original sources
PubMed1985

Immunohistochemical study of carcinoembryonic antigen in patients with colorectal cancer. Correlation with plasma carcinoembryonic antigen levels.

Using peroxidase-antiperoxidase (PAP) method, carcinoembryonic antigen (CEA) was demonstrated in conventionally processed colorectal cancer tissues. A new immunohistochemical grading for colorectal cancers based on the mode of the localization was made in an attempt to clarify the factors responsible for elevation of plasma CEA levels in colorectal cancer patients. Most of the patients with well differentiated adenocarcinoma, in which CEA was densely distributed along the apical surface but only rarely present along the basolateral surfaces of the carcinoma cells, had very low levels of plasma CEA, whereas all patients showing CEA distribution in the stroma as well as over the entire surfaces of the cancer cells and their cytoplasm showed high plasma CEA levels. In addition, there was a good correlation between the grading and presence of the blood vessel and lymphatic invasions. Thus, the appearance of CEA in the surrounding stroma, due to abnormal distribution of CEA on the basolateral plasma membrane of cancer cells, may play a significant role in the elevation of plasma CEA levels in colorectal cancer patients.

Adenocarcinoma↗
PubMed1985

Catheter-induced spasm during spontaneous attack of variant angina.

The simultaneous occurrences of spontaneous spasm and catheter-induced spasm during coronary angiography were obtained in 3 patients. Catheter-induced spasm was seen in the right coronary artery in 3 patients: 1 patient had spontaneous spasm in the distal right coronary artery and 2 patients had spontaneous spasm in the proximal left anterior descending coronary artery. These findings suggest that patients with variant angina may be susceptible to mechanical induction of spasm.

Angina Pectoris, Variant↗
PubMed1985

Postoperative immunization with peanut agglutinin-binding glycoprotein from Lewis lung carcinoma in mice.

The effects of postoperative immunization with purified tumor-associated antigens on the development of lung metastases following excision of the Lewis lung carcinoma (3LL) were investigated. Soluble tumor-associated antigens were isolated from 3LL cells by detergent solubilization and purified by affinity chromatography on peanut agglutinin (PNA)-agarose. The immunization with subcutaneous injections of PNA-binding glycoproteins (PNA receptors) at the early phase following excision of primary footpad tumors of 3LL resulted in a significant decrease in incidence and size of lung metastases. The delayed immunization, however, failed to suppress the development and growth of metastatic lung tumors after surgery. The suppression in postoperative development of lung metastases was found to depend on the scheduling of immunization following local tumor excision. Splenectomy performed simultaneously with amputation of the tumor-bearing leg completely abolished the decrease in the development and growth of postoperative lung metastases in immunized mice. The involvement of the lymphoid system in the control of metastatic tumor growth was also confirmed in an in vivo tumor neutralization assay utilizing the spleen cells.

Animals↗
PubMed1985

Antihepatotoxic actions of gingerols and diarylheptanoids.

The antihepatotoxic effects of gingerols, shogaols, diarylheptanoids and related analogues were assessed utilizing carbon tetrachloride- and galactosamine-induced cytotoxicity in primary cultured rat hepatocytes. Most congeners exhibited significant actions in these assay methods. The carbon tetrachloride assay appeared to be most useful in defining structure-activity relationships. The antihepatotoxic activity of gingerols and shogaols was dependent on the length of the side chain with the [7]- and [8]-companions eliciting the strongest activity. The gingerols exerted more intense activity than the corresponding shogaols. In the diarylheptanoids, introduction of hydroxyl groupings on the phenyl rings caused increased activity; however, the effect of the positions and number of hydroxyls on activity was variable depending on the carbon skeleton.

Alanine Transaminase↗
PubMed1985

[Capacity for IgE antibody production in ASK mice].

The capacity for IgE anti body production in ASK mice, which are highly sensitivity to anaphylactic shock, was compared with that in C3H and AKR mice. Three strains of mice were immunized with DNP-Ascaris mixed with aluminum hydroxide gel. IgE antibody to DNP in the sera was titrated by the rat 48-hour passive cutaneous anaphylaxis test. Maximum IgE titers of each strain of mice were 1: 2560 in C3H, 1: 1280 in ASK and 1: 640 in AKR. IgE antibody was detected in the sera until 170 days in C3H, 290 days in ASK and for not less than 320 days in AKR. These results suggest that the ASK mouse is a high responder strain for IgE antibody.

Anaphylaxis↗
PubMed1985

Effects of nipradilol (K-351) on alpha-adrenoceptor mediated responses in various isolated tissues.

Nipradilol competitively antagonized norepinephrine- or phenylephrine-induced contractile responses of guinea-pig thoracic aorta. These actions of nipradilol were about 6 times less potent than those of phentolamine. Nitroglycerin showed a non-competitive antagonistic action on norepinephrine-induced contractions of aorta. Furthermore, nipradilol competitively inhibited norepinephrine-induced contractions of rat vas deferens and dose-dependently reduced the phenylephrine-induced inhibitory responses in rabbit ileum. These antagonistic actions of nipradilol were 30 to 100 times less potent than those of phentolamine. Nitroglycerin did not appreciably affect these alpha-adrenoceptor mediated responses in rat vas deferens and rabbit ileum. The inhibitory action of clonidine on the twitch contraction of rat vas deferens produced by intramural stimulation was only slightly antagonized by nipradilol (pA2 = 5.4). Nipradilol and nitroglycerin showed a non-competitive antagonistic action on clonidine-induced contractions of canine saphenous vein after the exposure to phenoxybenzamine, while phentolamine competitively inhibited the clonidine responses. These results suggest that nipradilol possesses an alpha 1-adrenoceptor blocking action; it possesses very weak or practically no presynaptic alpha 2-blocking activity but shows a non-competitive antagonistic action on postsynaptic alpha 2-adrenoceptor mediated contractile responses.

Adrenergic alpha-Antagonists↗
PubMed1985

[Studies on sensitivity test with clonogenic assay against chemo-endocrine therapy for human breast cancer].

The sensitivity of human breast cancer cells against estradiol-17 beta (E2), tamoxifen (TAM) and adriamycin (ADM) was investigated using the modified stem cell assay method by Hamburger and Salmon. Two hundred and six specimens, 145 from primary breast cancer and 61 from metastatic lesions, were placed in culture. Ninety eight specimens of them (47.1%) successfully formed more than 5 colonies per 5 X 10(5) cells plated. In 47 specimens (22.8%), more than 20 colonies were formed, which made it feasible to perform meaningful sensitivity test. Results obtained were as follows: Sensitivity against E2. More than 150% increase of colony formation was observed in the 36.7% cases of ER(+) group and in the 22.1% cases of ER(-) group. Sensitivity against TAM. When more than 60% inhibition in % colony formation was applied to cut off line, response rates for TAM were 21.7% in all cases, 26.7% in ER(+) group and 12.5% in ER(-) group, respectively. Sensitivity against ADM. When more than 50% inhibition in % colony formation was applied to cut off line, response rate for ADM was 26.7% in all cases. Higher response rate was observed in ER(-) group than in ER(+) group. Response rates of sensitivity test for TAM or ADM were very similar to the results obtained in the clinical use of TAM or ADM.

Breast Neoplasms↗
PubMed1985

[Intraparenchymal epidermoid cyst of the right frontal lobe: a case report].

Epidermoid cysts are most likely to occur at the cerebellopontine angle or in the suprasellar region. Intracerebral epidermoid cysts are rare and only 18 cases have been reported in the literature. This report presents a case of epidermoid cyst which developed in the right frontal lobe. A 24-year-old woman was admitted to our clinic on April 24, 1983 because of generalized convulsions. On admission no neurological abnormalities were found except for absence of the venous pulsation in an ophthalmoscopic study. A plain roentgenogram revealed a crescent shaped calcification in the right frontal lobe. An X-ray CT scan showed a round low density area (Hounsfield units + 12) surrounded by irregular ring-like high density spots in the right frontal lobe. Right carotid angiography showed round shift of the anterior cerebral artery to the left. The horizontal portion of the middle cerebral artery was stretched and lenticulostriate arteries were shifted laterally. Abnormal vascularization was not found. On May 11th 1983 a right fronto-temporal craniotomy was performed. Partial corticotomy was done along the cortical sulcus and the tumor was resected. The histological diagnosis was epidermoid cyst. In general an X-ray CT scan shows a low density area with irregular margin. Usually the low density area revealed -8 approximately +30 of Hounsfield units. Neither perifocal low density area nor enhancement by contrast medium is observed.

Adult↗