The morphology of 6 bacteriophages isolated from Staphylococcus saprophyticus was studied by electron microscopy. Phages had isometric heads and non-contractile tails, thus belonging to the Siphoviridae family. They were subdivided into two morphological groups based on head diameter and tail length. Type I, represented by phages 1154A and 1405, and characterized by an unusually small capsid, may be a new species.

Capsid↗

PubMedDate not supplied

Computerized management of a medical bacteriology laboratory using real-time processing.

The computer system of the Bacteriology Laboratory at the Hôpital Cardiologique in Lyon is one of the elements in the computer management of patient data. It dovetails in with the various units for admission, patient location, ward surveillance, outpatient appointments. The main objectives are as follows: to improve quality of sample information, to reduce clerical work and accelerate information circulation, to widen the scope of epidemiological research. Computers can register samples, edit laboratory work sheets, entry lists, record results, generate reports, give a visual listing of all analyses performed before a patient leaves hospital, list pending analytical results, make available in the total system all laboratory-validated information, produce automatic billing of analyses and statistical editing. The Bacteriology Laboratory has three visual display units and two printers at its disposal. Computer laboratory management has been operational since May 1977. First results are satisfactory and suggest extension of the system to other laboratories and handling of quality control.

Bacteriology↗

PubMedDate not supplied

[Prevention of infections in operating rooms].

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Air Microbiology↗

PubMedDate not supplied

[Place of Staphylococcus aureus in the bacterial isolations of the University Hospital Center of Dakar. Preliminary results of serotyping and phage typing].

Out of 18 837 strains isolated between 1972 and 1980, by the laboratory of bacteriology of the Dakar Teaching Hospital, 2 925 strains of Staphylococcus aureus have been identified. This germ was the cause of 14,4 p. 100 of all diagnosed septicemia, ranging second in frequency after Salmonella. It was the most frequent germ in pura (54,8 p. 100 of strains) and rated 29,2 p. 100 of the strains isolated from pleural fluids. It was less frequent in bacterial meningitidis (2,5 p. 100) and in infections of the urinary tract (3,6 p. 100). About one hundred coagulase + strains were studied further. A clumping factor was found in 98 p. 100 of the strains (Staphyslide test). In addition, serotyping and lysotyping were carried out: --80,8 p. 100 of strains were lysotyped --25,6 p. 100 of them belonging to type I --7,7 p. 100 to type II --10,3 p. 100 to type III --33,3 p. 100 belonging to two or three types --11,5 p. 100 to I and II --16,7 p. 100 to I and III --5,1 p. 100 to I and II and III Some strains gave positive responses with 94-96 and 187 phages.

Bacteriophage Typing↗

PubMedDate not supplied

[New bacteriophages of Staphylococcus epidermidis: evaluation of their epidemiological value].

Coagulase-negative staphylococci, and in particular Staphylococcus epidermidis, are now being recognized as causing human infections with increasing frequency; the absence of an internationally accepted system of phage-typing for coagulase-negative staphylococci led us to isolate new phages. Fifty strains of S. epidermidis isolated from human infections were induced with mitomycin C: eight phages (41, 63, 118-II, 138, 245, 336, 392 and 550) were isolated. These phages were propagated on five different strains of S. epidermidis. Their phages were propagated on five different strains of S. epidermidis. Their lytic activity as studied on 561 strains. Phages 336, 392 and 550 had a different host-range and different propagative strains; they typed 93% of the strains susceptible to the 8 phages. The other phages had an activity similar to that of phage 336. Twenty-one per cent of non-epidemic strains were susceptible to at least one of the three phages. The reproducibility, specificity and discriminatory power of these phages suggest they may be a useful addition to previously recognized phages.

Bacteriophage Typing↗