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Biomedical subjects

Y Asano

Publications and source records attributed to Y Asano.

At least 775 records · Page 43Linked to original sources
PubMed1980

Uremic pericardial effusion: detection and evaluation of uremic pericardial effusion by echocardiography.

Echocardiography (UCG) was performed prior to and during hemodialysis therapy to detect and evaluate volumetrically the grade of pericardial effusion in 150 patients with chronic renal failure. The actual incidence of uremic pericardial effusion during the observation period was 62% and was higher during the first 3 months of therapy than at later stages. Pericardial effusion was classified from grade 0 (no effusion) to grade 5 (massive effusion). We compared the grade of effusion with laboratory test results, blood pressure, cardiothoratic ratio, left atrial and ventricular dimensions, and thickness of the intraventricular septum and left ventricular posterior wall. Significant differences between the patients with and without effusion were detected in the degree of systolic hypertension, dilatation of the left atrial chamber, anemia and hypoproteinemia. There was no correlation between the grade of effusion and creatinine, uric acid and calcium levels and changes of body weight. Our results confirm that insufficient dialysis as indexed by the degree of hypoproteinemia, anemia, hypertension and central overhydration, may play a role in the etiology of uremic pericardial effusion.

Adolescent↗
PubMed1979

Relationship between Na+-dependent respiration and Na+ + K+-adenosine triphosphatase activity in the action of thyroid hormone on rat jejunal mucosa.

Administration of three successive doses of triiodothyronine (T3) (50 micrograms/100 g body wt), given on alternate days to thyroidectomized and euthyroid rats, stimulated oxygen consumption (QO2) and Na+ transport-dependent respiration (QO2 [5]) in the stripped jejunal mucosa, a preparation that consisted mostly of epithelial cells. The increase in QO2(t) accounted for 57% of the increment in QO2 in the transition from the hypothyroid to the euthyroid state and for 29% of the increment in the transition from the euthyroid to the hyperthyroid state. Administration of T3 to hypothyroid rats also increased the yield of epithelial cells. Injection of T3 into thyroidectomized and euthyroid rats increased the specific activity (at Vmax) of the (Na+ + K+)-dependent adenosine triphosphatase (NaK-ATPase) in jejunal crude membrane preparations. No significant change was recorded in the activity of Mg-ATPase in the same preparation. The ratio of QO2/NaK-ATPase and QO2(t)/NaK-ATPase in the various thyroid states remained constant, indicating proportionate increased in the respiratory and enzymatic indices. The effect of administration of T3 to thyroidectomized rats on the number of NaK-ATPase units (recovered in the crude membrane preparation) was estimated by: (a) Na+ + Mg++ + ATP-dependent binding of [3H]-ouabain to crude membrane fractions, and (b) the amount of the phosphorylated intermediate formed in the NaK-ATPase reaction from AT32P(gamma). Estimates were obtained of the maximal number of [3H]ouabain binding sites (Nm) and dissociation constants (Kd). Nm for [3H]ouabain and Nak-ATPase specific activity increased to about the same extent after T3 administration to thyroidectomized rats, with no change in the apparent Kd values. The amount of phosphorylated intermediate formed in jejunal crude membrane preparations also increased significantly. Thus, thyroid hormone administration may increase the number of active Na+pump sites in the plasma membrane. The apparent increase in the number of Na+ pump sites also correlated with the hormone dependent increases in QO2 and QO2(t).

Animals↗
PubMed1979

Comparison of 4 serological tests--complement fixation, neutralization, fluorescent antibody to membrane antigen and immune adherence hemagglutination--for assay of antibody to varicella-zoster (V-Z) virus.

Four tests for antibody to varicella-zoster (V-Z) virus were compared; these were tests of complement fixation (CF), neutralization (NT), fluorescent antibody to membrane antigen (FAMA) and immune adherence hemagglutination (IAHA). Fifty-two sera from patients with varicella and zoster and from recipients of live varicella vaccine were examined by the 4 tests. The CF test was least sensitive, but the antibody titers by the NT, FAMA and IAHA tests were roughly comparable. The IAHA test was the simplest and fastest to perform, and appeared suitable for routine serological assay to V-Z virus. The correlation between the IAHA antibody titer and susceptibility of individuals to clinical varicella was investigated retrospectively using sera obtained during 2 outbreaks of varicella in an institution for children, where all the unvaccinated children had developed varicella symptoms. Most of the 25 pre-exposure sera from unvaccinated children examined by the IAHA test had tiers of less than 1:2. In contrast, all the 23 sera from vaccinated children who did not develop varicella had detectable antibody titers of 1:2 to 1:64. These results indicate that the IAHA titer reflects the susceptibility or resistance of individuals to clinical varicella.

Antibodies, Viral↗
PubMed1979

Studies on the polypeptides of varicella-zoster (V-Z) virus. 1. Detection of varicella-zoster virus polypeptides in infected cells.

Virus-induced polypeptides in cells infected with varicella-zoster virus (VZV) were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography. When human embryonic lung (HEL) cells infected with the Oka strain of VZV were labelled with 35S-methionine or 14C-glucosamine from 40 hr to 46 hr after infection, at least 18 VZV-induced polypeptides and 10 glycoproteins could be identified in the infected cells. The molecular weights of the polypeptides and glycoproteins ranged from about 145,000 to 23,000, and from about 105,000 to 48,000, respectively. Lysates of VZV-infected cells were treated with specific antisera prepared in green monkeys or guinea-pigs, and analysed by SDS-PAGE and fluorography. In all, 33 polypeptides (with molecular weight of about 145,000 to 22,000) and 13 glycoproteins (molecular weight, about 105,000 to 38,000) were found in the immunoprecipitates. None of these polypeptides and glycoproteins were detected when infected cells cultured in the presence of phosphonoacetic acid (PAA) were treated in the same way.

Cell Line↗
PubMed1979

[A case of spontaneous hematomyelia (author's transl)].

A case of spontaneous hematomyelia was reported with a review of the literature. A 39-year-old woman, with sudden onset of pain in her left neck and arm 13 days previously, presented with a progressive flaccid tetraplegia below C5, dissociated sensory loss from C7 through Th4 dermatome and a minimal deep sensory loss in her right leg. Myelography revealed a symmetrical enlargement of the spinal cord at the leve of C5, C6 and C7. Laminectomy was performed and intramedullary blood clot, which was located in the region of C6 and C7, was evacuated. However, there were no vascular anomalies not only in macroscopic findings but also in histological examination. The postoperative course was uneventful and the patient gradually improved in her motility. At 5 month' follow-up examination she continued to do well with a minimal sensory loss of all modalities below the level of C7. Report of 16 patients with spontaneous hematomyelia including our case have been reviewed. Incidence of spontaneous hematomyelia without vascular anomalies or hemorrhagic diathesis very rare, being found in only 4 cases. In cases with good outcome the intramedullary hematoma was found to be located below the lower cervical region and limited within 2 spinal segments in its extent. Good postoperative results were obtained in 5 out of 7 patients who received surgical intervention, suggesting an essential importance of early diagnosis and surgery.

Adult↗
PubMed1978

Increased cell membrane permeability to Na+ and K+ induced by thyroid hormone in rat skeletal muscle.

Thyroid hormone (T3) increased Na+ dependent respiration accompanied by an increase in NaK-ATPase activity. Administration of T3 increased intracellular K+ concentration and Na/K ratio in thyroidectomized rats, and the Na+ efflux rate constant incubated in oxygenized Na+, K+-Ringers in euthyroid rats. However, the magnitude of the changes in intracellular K+ concentration was modest or invisible in comparison to the changes in QO2(t) and NaK-ATPase activity. The Na+ and K+ efflux rate constants in K+-free +ouabain Ringers were increased by T3 in both thyroidectomized and euthyroid rats. Thus, thyroid hormone stimulates not only Na pump but also the permeability of cell membrane to Na+ and K+. The both effects might contribute to the thyroid thermogenesis.

Adenosine Triphosphatases↗
PubMed1978

Blood pressure in stroke-prone SHR (SHRSP) exposed to unusual environmental temperature.

1) It is generally accepted that the adequate environmental temperature to rear rats is about 23 degrees C, but the adequate temperature that directly contacts to the animal's skin seems to be about 30 degrees C. 2) The blood pressure increased on cold exposure or stimulation. There was a case that the blood pressure increased slightly on hot exposure. This suggests that it is necessary to pay close attention to the condition of warming-up at the indirect measurement of blood pressure. 3) Apoplexy seemed to occur earlier at the low environmental temperature, and the blood pressure seemed to increase also at the high temperature. However, these temperature effects on blood pressure and the incidence of stroke were not so obvious as observed in rearing SP with 0.9% salt solution for drinking.

Animals↗