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Biomedical subjects

Y Asano

Publications and source records attributed to Y Asano.

At least 757 records · Page 42Linked to original sources
PubMed1982

Fronto-ethmoidal mucocele--observation of its mode of enlargement.

From a study of the roentgenographic findings of fronto-ethmoidal mucoceles and anatomical specimens of the paranasal sinuses the authors surmised that many of the fronto-ethmoidal mucocele probably originate either in one of the deeply seated supra-orbital frontal or ethmoidal recesses, or in an ethmoid cell whose ostium has been obstructed by postoperative, post-traumatic or post-inflammatory osteoneogenesis or cicatrization. When isolated, the cell with secreting mucosa would increase its cavity slowly but steadily, and eventually blocks the naso-frontal duct from without. The characteristic localization of the mucoceles, frequent erosion of the superomedial wall of the orbit with inferolateral displacement of the globe, our roentgenographic findings of a mucocele within the frontal sinus, pseudostratified columnar epithelium that lines the mucocele, occasional multilocularity, and the long interval, over ten years, that is required for the mucocele to show any symptoms in most instances would support this hypothesis.

Adult↗
PubMed1981

Role of the major histocompatibility complex in T cell activation of B cell subpopulations. Major histocompatibility complex-restricted and -unrestricted B cell responses are mediated by distinct B cell subpopulations.

The present study has evaluated the identity of the B cell subpopulations participating in T dependent antibody responses that differ in their requirements for major histocompatibility complex-restricted T cell recognition. In vitro responses of keyhole limpet hemocyanin (KLH)-primed T cells and trinitrophenyl (TNP)-primed B cells were studied to both low and high concentrations of the antigen TNP-KLH. It was first demonstrated that for responses to low concentrations of TNP-KLH, (A x B)F(1) {arrow} parent(A) chimeric helper T cells were restricted in their ability to recognize parent(A) but not parent(B) H-2 determinants expressed by both B cells and antigen-presenting cells (APC). In contrast, at higher antigen concentrations, helper T cells were not restricted in their interaction with B cells. It was then determined whether these observed differences in T cell recognition resulted from the activation of distinct B cell subpopulations with different activation requirements. At low concentrations of TNP-KLH it was demonstrated that Lyb-5(-) B cells were activated, and that it was thus the activation of the Lyb-5(-) subpopulation that required T cell recognition of B cell H-2 under these conditions. In contrast, responses to high concentration of antigen required the participation of Lyb-5(+) B cells, and these Lyb-5(+) B cells were activated by a pathway that required H-2- restricted T cell interaction with APC, but not with B cells. The findings presented here have demonstrated that Lyb-5(-) and Lyb-5(+) B cells constitute B cell subpopulations that differ significantly in their activation requirements for T cell-dependent antibody responses to TNP-KLH. In so doing, these findings have established that the function of genetic restrictions in immune response regulation is critically dependent upon the activation pathways employed by functionally distinct subpopulations of B, as well as T, lymphocytes.

Animals↗
PubMed1981

Induction of idiotype-bearing, nuclease-specific helper T cells by in vivo treatment with anti-idiotype.

Treatment of BALB/c mice with purified pig anti-(BALB/c anti-nuclease) anti-idiotypic antibodies has been found to induce the appearance of idiotype-bearing immunoglobulins (Id') in the serum of these mice in the absence of detectable antigen binding activity. This phenomenon appeared to require T cells in the hosts because no Id' was detected in the serum of nude mice similarly treated. Furthermore, the spleens of BALB/c mice treated with anti-idiotype were found to contain helper T cells capable of providing help in an in vitro plaque-forming cell response to trinitrophenyl-nuclease equivalent to that provided by helper T cells from the spleens of nuclease-primed animals. Helper T cells from both anti-idiotype-treated and nuclease-treated animals were found to be antigen-specific and to be similarly susceptible to elimination by treatment with anti-idiotype plus complement. Therefore, treatment with both antigen and anti-idiotype appeared to prime similar populations of antigen-specific helper T cells, while having different effects on the induction of antibody. These findings are consistent with the network theory of receptor interactions in the immune response, and may provide a means for studying individual cell populations involved in such interactions.

Animals↗
PubMed1981

Soluble skin test antigen of varicella-zoster virus prepared from the fluid of infected cultures.

A soluble varicella virus antigen for skin testing was developed using culture fluid from varicella-zoster virus-infected human diploid cells. The culture fluid was centrifuged at a high speed (100,000 g for 2 hr) to remove viral particles, and the supernatant, after heating at 56 C for 30 min, was used as the soluble skin test antigen. No virion or nucleocapsid was found by electron microscopy, and very little 3 H-labeled DNA in the soluble antigen hybridized with viral DNA. By immunoprecipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and fluorography, several viral glycoprotein bands were detected in the soluble antigen. In a comparative skin test on 10 medical students and 10 children using soluble and crude antigens prepared from culture fluid or infected cells, the skin reaction to the soluble antigen tended to be less than that to the crude antigens, but the former reaction correlated well with a history of varicella and the presence or absence of virus-neutralizing antibodies.

Antigens, Viral↗
PubMed1981

Ia antigens on antigen-presenting cells do not carry the same Ia specificities as detected on suppressor and helper T cells.

The passage of spleen cells through a tightly packed nylon wool column partially separated two populations of cells required for the antigen-induced secondary proliferative response of T cells. The proliferating T cells were in the nylon wool column-passed cell population, but the addition of a small number of nylon-wool-adherent cells was required for their maximal proliferative response. Such adherent cells were non-T, non-B, Ia antigen-positive cells serving as antigen-presenting cells. Ia determinants on this cell type are encoded in two separate I subregions, I-A and I-E/C, and they are expressed simultaneously on the same cells. Antisera specific for I-J subregion gene products having as a known activity the killing of antigen-specific suppressor and helper T cells were unable to kill antigen-presenting adherent cells. The results indicated that at least I-J subregion gene products expressed on suppressor and helper T cells are not present on antigen-presenting accessory cells. The Ia specificities detected on accessory cells follow the cross-reactive pattern found in Ia molecules of B cells, and thus antigen-presenting cells carry Ia antigen identical to those of B cells, and not those of T cells.

Animals↗
PubMed1981

[Changes in serum levels of alkaline phosphatase, calcium, and inorganic phosphate following antiepileptic therapy (author's transl)].

Relationship of antiepileptics to serum alkaline phosphatase (ALP), serum calcium (Ca) and inorganic phosphate (P) were studied in 172 epileptic patients treated with antiepileptics for clarifying the related factors to produce osteomalacia following antiepileptics administration. Laboratory findings of ALP, Ca, and P were compared with normal value and determined as abnormal by exceeding the normal limit (mean +2SD (ALP), mean-2SD (Ca, P)). The following results were obtained: 1) In the 172 patients, 20 cases (11.6%) showed abnormal value of ALP, 12 cases (7.0%) of Ca and 41 cases (23.8%) of P (Single abnormal groups). On the other hand, 47 cases (27.3%) were found abnormal in two or three of ALP, Ca and P (Combined abnormal group). The rest 52 cases (30.2%) of the patients showed all normal value (Normal group). 2) Abnormal value of ALP and/or Ca were observed mostly before 20 years of age. The patients with abnormal P were more distributed in age than others. 3) The earlier and/or the longer administration of antiepileptics is prone to produce the more abnormal value. 4) Acetazolamide, metharbital, primidone, carbamazepine and mephobarbital were more used in combined group than in normal group, and polypharmaceutical use of these antiepileptics was supposed to be related to the abnormality of ALP, Ca and P. There was no different use of diphenyl-hydantoin and phenobarbital in frequency and amount between combined group and normal group. 5) Mental retardation and epileptic personality changes were observed more frequently in combined abnormal group than in normal group. 6) No significant relations were observed between clinical seizure types, seizure frequencies and abnormal laboratory findings.

Adolescent↗
PubMed1980

The age distribution of neutralizing antibodies against varicella-zoster virus in healthy individuals.

A seroepidemiological survey of varicella was made in the Nagoya area by the neutralization (NT) test. Of 1,473 recorded cases of varicella, 81.4% were under 6 years old and 9.6% were under one year old; of the 168 recorded cases under one year old, about 30% were under 5 months old. Examination of 11 pairs of mother and cord sera and 13 pairs of mother and infant sera showed that transfer of NT antibody was in general good, even in babies that were small for their age or smnall at birth after 28 weeks gestation. The transferred maternal antibody decreased rapidly, becoming undetectable in babies of 4 months old. Then with increase in age the percentgage of seropositive children gradually increased, being 53.3% at 4 to 5 years old, and 100% in those of over 9 years old, with a temporary decrease in young adults in their twenties.

Adolescent↗
PubMed1980

Spread of varicella in hospitalized children having no direct contact with an indicator zoster case and its prevention by a live vaccine.

Varicella spread from a child with zoster to a total of 3 susceptible infants in another room in a children's ward, although they had been strictly isolated. To prevent spread of the disease, the staffs and patients were doing their own washing and no source of natural infection could be found. The cases indicate that it is difficult to predict nosocomial varicella infection or to prevent spread of the disease simply by isolation in a children's ward. A total of 11 other children without history of varicella in the ward were given live varicella vaccine before or immediately after this event. None of these children developed symptoms of varicella and all the susceptible children who were vaccinated showed an antibody response.

Adolescent↗
PubMed1980

Studies on the polypeptides of varicella-zoster (V-Z) virus. II. Syntheses of viral polypeptides in infected cells.

Synthesis of varicella-zoster virus (VZV)-induced polypeptide was examined at intervals after infection by immunoprecipitation, SDS-polyacrylamide gel electrophoresis (PAGE) and fluorography. Human embryonic lung (HEL) cells were inoculated with VZV-infected cells at a ratio of about 1 infected cell to 5 uninfected cells and pulse-labelled with 35S-methionine or 14C-glucosamine. Under this experimental condition, virus-induced protein and glycoprotein began to appear 4 to 6 and 18 to 20 h after infection, respectively, and a capsid protein (MW 145,000) was prominent 18 h after injection. The synthesis of these proteins reached maxima at 46 to 48 h after infection. In all, 34 polypeptides and 10 to 13 glycoproteins with molecular weight of 145,000 to 22,000 and 115,000 to 38,000, respectively, were detected. A few polypeptides (MW 130,000, 95,000, 72,000) were detected in infected cells treated with phosphonoacetic acid (PAA) at an early stage of infection. Proteins and glycoproteins of virion of VZV were analyzed by immunoprecipitation with antisera against partially purified virion of VZV. At least 12 polypeptides with MW of 145,000 to 35,000 and 7 glycoproteins (MW 105,000, 90,000, 84,000, 72,000, 70,000, 60,000 and 56,000) were identified. A polypeptide with a molecular weight of 145,000 was considered to be the major capsid protein.

Cell Line↗