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Biomedical subjects

Y Alarie

Publications and source records attributed to Y Alarie.

At least 73 records · Page 4Linked to original sources
PubMed1983

Single-tracer technique to evaluate pulmonary edema and its application to detect the effect of hexamethylene diisocyanate trimer aerosol exposures.

Two hours after a four-hour exposure to hexamethylene diisocyanate trimer (HDIt) aerosol between 2.5 and 39 mg/m3, mice were injected iv with 51Cr-EDTA (chromium ethylenediaminetetraacetate). Ten minutes later the lung was lavaged. A larger amount of 51Cr-EDTA was detected in the lung lavage of HDIt mice than of controls in a concentration-related fashion. The concentration-response curve was shifted to the left compared with that constructed using lung weight increase as response. Kinetic studies of the plasma level of 51Cr-EDTA revealed a three-exponential profile in normal mice, and similar plasma levels were obtained with mice exposed to 18-24 mg/m3 HDIt. However, both the amount of 51Cr-EDTA in the alveolar space and concentration in the pulmonary extravascular compartment were higher in HDIt-exposed mice than in controls. The data of 51Cr-EDTA distribution in the lung were fitted with a three-compartment model. According to the model, HDIt exposures increase the permeability constants of 51Cr-EDTA transport into the alveolar space from blood which accounts for the larger amount of 51Cr-EDTA in lung lavage of HDIt-exposed mice. This 51Cr-EDTA injection and lung lavage technique is a sensitive method for detecting pulmonary edema.

Aerosols↗
PubMed1981

Multicellular in vivo sister-chromatid exchanges induced by urethane.

Following urethane inhalation exposure, clear dose--response relationships were apparent in all cell types examined in hepatectomized and intact mice. At concentrations of 0.1 mg/l and higher, induced SCE frequencies were linearly related to log urethane concentrations. No significant differences in SCE response between like cell types of hepatectomized and intact mice were apparent. In hepatectomized mice, there was no significant difference in the SCE response of regenerating liver and alveolar macrophage cells. However, bone-marrow response was significantly lower (p = 0.01). Likewise, in intact mice bone marrow response was significantly lower than in alveolar macrophages (p = 0.01). Inhalation and intravenous infusions of the same total dose of urethane (193 mg/kg) administered over a 4-h period produced comparable SCE responses in all cell types. However, a single intraperitoneal injection of 193 mg/kg just prior to BrdU infusion produced significantly higher SCE frequencies in bone marrow (alpha = 0.01), and alveolar macrophages (alpha = 0.05) of intact mice than did the equivalent inhalation dose. Intraperitoneal injections produced similar results in 2-month-old mice as in 4-month-old mice. However, regardless of the route of administration SCE frequencies in regenerating liver and/or alveolar macrophages were significantly higher than in bone marrow.

Aerosols↗
PubMed1981

Dose-response analysis in animal studies: prediction of human responses.

An animal bioassay has been used to evaluate a series of airborne chemicals for their sensory irritating properties to the upper respiratory tract. Teh results obtained can be used to rank their potency. An attempt has been made to predict "safe" levels of exposure for humans on the basis of this short-term assay. A good correlation was obtained between the predicted "safe" levels of exposure and current Threshold Limit Values established for industrial exposures.

Air Pollutants↗
PubMed1981

Potency of some carbamates as multiple tissue sister chromatid exchange inducers and comparison with known carcinogenic activities.

The ethyl, ethyl N-hydroxy, isopropyl, and methyl esters of carbamic acid were examined for their abilities to induce sister chromatid exchanges (SCEs) in alveolar macrophages, bone marrow, and regenerating liver cells of C57BL/6J X DBA/2J F1 mice. The relative potencies in inducing SCE, ethyl greater than ethyl N-hydroxy- greater than isopropyl, paralleled previously described activities for induction of lung adenomas in strain A mice. The noncarcinogenic methyl carbamate was inactive in the SCE assay. Relative to bone marrow, regenerating liver and alveolar macrophage cells demonstrated increased susceptibility to carbamate-induced SCE. Of all carbamates studied, only the directly active compound, ethyl N-hydroxycarbamate, produced distinctly different responses in extrahepatic tissues of hepatectomized and intact mice. In intact mice, SCE levels induced by ethyl carbamate in bone marrow and alveolar macrophage cells were not significantly different whether assay followed the last of 12 (three times weekly) serial injections of 2.2 mmol/kg each or after a single injection of 2.2 mmol/kg. Linear regression relationships of log ethyl carbamate versus log SCE or log adenoma response were found to be parallel with the two assays having similar sensitivities.

Animals↗
PubMed1980

Evaluation of sensory irritation from some common industrial solvents.

An animal bioassay model was used to determine the potency of some common industrial solvents as sensory irritants. Acetone was found to be the least potent and ethyl acetate the most potent. A series of alcohols tested, from methanol to pentanol, revealed an increase in potency with the increasing number of carbons.

Acetaldehyde↗