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Biomedical subjects

Y Abe

Publications and source records attributed to Y Abe.

At least 1,135 records · Page 63Linked to original sources
PubMed1981

[Studies on the immune state evaluated by various skin antigens in patients with cervical cancer (author's transl)].

Cutaneous delayed hypersensitivity (CDH) tests, using 6 antigens (PHA, PPD, SK-SD, Candida, Mumps and DNCB) were performed in 260 patients with cervical cancer and the following results were obtained. 1) Decrease in incidence of positive CDH (PHA, PPD and DNCB) was found as the clinical stage advanced. Especially, decrease in incidence of positive CDH (DNCB and PHA) was found in stage III and IV. 2) There was no relationship between the incidence of positive CDH (SK-SD, Candida and Mumps) and clinical stage but, decrease in incidence of these tests was found in stage III and IV. 3) In comparison on numbers of positive CDH, there was no significance in control groups and cervical cancer patients. 4) Incidence of advanced invasion of primary lesion (gamma and delta type) was 83.7% in DNCB-negative group who were treated with curative operation. 5) Incidence of regional lymph node metastasis was 42.9% in Candida-negative group, whereas, 16.2% in Candida-positive group who were treated with curative operation.

Antigens↗
PubMed1980

[Studies on angiotensin II receptors in the adrenal glands and in the aorta (author's transl)].

The angiotensin II receptor in the rabbit, rat and human adrenal gland and that in the rabbit and rat aorta were studied by using [3H]-angiotensin II ([3H]-AII). The adrenal glands and the aorta of each species were centrifuged at 20,000Xg for 30 min, and pellets which contained 95% of binding sites were collected and diluted by a buffer [20 mM Tris-HCl buffer (pH 7.4), 120 mM NaCl, 0.2% BSA, 1 mM EDTA] and used as a membrane fraction. The binding study was done by incubating the membrane fraction and [3H]-AII. After incubation at 25 degrees C for 30 min, the binding of the membrane fraction and [3H]-AII reached the equilibrium state, and free and bound angiotensin II were separated by 0.48 micrometer filters. Protein concentration was determined according to the method of Lowry. The binding sites fulfilled the criteria of the receptor which has organ and structure specificities, high affinity and reversibility. We could thus measure angiotensin II receptor in this study correctly and concisely. The properties of the angiotensin II receptor were analysed by Scatchard plot. The number of rabbit adrenal receptors (12833 +/- 2115 x 10(-15) mol/mg.protein) (M ++/- SD) was greater than that of the rat (1780 +/- 166 x 10(-15) mol/mg.protein) and that of the human (356 +/- 124 x 10(-15) mol/mg.protein). But the dissociation constant of all species was the same. The number of aorta binding sites was less than that of the adrenal in rabbits and rats. But the dissociation constant of rabbits and rats aorta binding sites was quite similar to that of adrenal glands.

Adrenal Glands↗
PubMed1980

Renal effects of 8-substituted derivatives of adenosine 3',5'-cyclic monophosphate in dogs.

The renal effects of nine, 8-substituted derivatives of 3',5'-cyclic monophosphate were studied in anesthetized mongrel dogs. Infusion of the compounds into a renal artery resulted in an increase of renal blood flow without any effect on blood pressure. Diuretic and natriuretic effects are evident with 6 of the 9 derivatives. As these 8-substituted analogues exert renal effects in a manner similar to that seen with the parent nucleotide, cyclic adenosine 3',5'-monophosphate, they may serve as useful pharmacological agents in vivo.

Animals↗
PubMed1980

Effects of 4-pentenoic acid and furosemide on renal functions and renal uptake of individual free fatty acids.

1. Effects of 4-pentenoic acid (4-PA), an inhibitor of fatty acid oxidation, on renal fatty acids (FFA) were investigated using mongrel dogs, and the results compared with findings in the case of furosemide. 2. Continuous infusion of 4-PA at a rate of 0.66 mumoles/kg.min into the renal artery resulted in a marked reduction of glomerular filtration rate after 45 min. Sodium reabsorption rate tended to decrease at 30 min and a significant decrease was seen after 45 min. With decrease in the renal sodium reabsorption rate, the urine flow increased about 3 times, and there was a marked natriuresis. 3. Concentrations of individual FFA in the arterial and renal venous plasma were determined by gas-liquid chromatographic analysis. In the control period, data from 25 dogs showed renal uptake of palmitic, stearic, oleic, and linoleic acids to be approximately 40, 15, 25, and 10 nmoles/g.min, respectively, and the uptake of palmitic and oleic acids was depressed during the natriuresis following administration of 4-PA and furosemide. 4. These results suggest that fatty acids play an important role in the energy supply in renal tubular sodium transport.

Animals↗
PubMed1980

Formation of heterotopic granule cell in mouse cerebellum after neonatal administration of cytosine arabinoside.

The mice, ICR-JCL strain, were injected s.c. with 30 mg/kg body weight of cytosine arabinoside at the age of 2, 3, and 4 days. The external granular layer of these mice was destructed selectively, and subsequently these mice developed abnormal cytoarchitecture in the cerebellum, such as disarrangement of Purkinje cells and heterotopic granule cells in the molecular layer. This study was undertaken to elucidate the mechanism in the formation of heterotopic granule cells. In the cerebellum of the treated mouse, some mossy fibers and glomerular collaterals of climbing fibers extended abnormally even into the molecular layer by the age of 10 days, since no granule cells migrated to the inner granular layer until about 10 days when granule cell production started again in the regenerated external granular layer. Subsequently, these fibers, i.e. axons which extended into the molecular layer, established synapses in the molecular layer with the dendrites of migrating granule cells. These granule cells had no need to migrate to the inner granular layer, and so they remained in the molecular layer as heterotopic granule cells.

Age Factors↗
PubMed1980

Effects of calcium ionophore, A23187, on prostaglandin E2 and renin release in dogs.

Effects of calcium ionophore, A23187, on prostaglandin E2 and renin release, and the interrelationship were investigated in anesthetized dogs. PGE2 concentration in arterial and renal venous plasma was measured by radioimmunoassay, and secretion rate (pg/g-min) from the kidney was calculated. The renin secretion rate (ng/g-min) was calculated simultaneously. Intrarenal infusion of A23187 at a rate of 0.5 mg/min for 2 min resulted in a significant increase in RBF. PGE2 secretion rate increased from 61 +/- 26 to 1012 +/- 566 1222 +/- 500 and 388 +/- 125 at 5, 9 and 17 min. Renin secretion rate also increased rom 1.4 +/- 0.8 to 2.3 +/- 1.3, 5.8 +/- 2.7 and 5.9 +/- 1.6 at 5, 9 and 17 min. However, systemic administration of A23187 at a rate of 0.5 mg/min for 2 min did not alter the parameter. Thus, intrarenal infusion of A23187 affects RBF, renin release and PGE2 release directly within the kidney. In indomethacin treated dogs (5 mg/kg, i.v.) intrarenal infusion of A23187 significantly decreased RBF. The renin release with A23187 was also abolished with indomethacin as well as PGE2 release. These results suggest that calcium mobilization within the kidney plays an important role in PGE2 synthesis and release, and that the renal vasodilation and renin release are secondary effects of renal PGs synthesis and release.

Animals↗
PubMed1980

Effects of prostaglandin I2 and E2 on renal hemodynamics and function and renin release.

The effects of PGI2 and PGE2, given in the same doses were compared as to renal hemodynamics and functions and renin release. The same experimental conditions were used for both compounds and experiments were carried out in pentobarbital anesthetized dogs. Adrenergic influences were excluded by renal denervation. Given intrarenally at doses at 0.1 microgram/min and 1 microgram/min, PGE2 caused significant increase in RBF, UF and UNaV, but had no effect on BP and GFR. Intrarenal infusion of PGI2 at a rate of 1 microgram/min resulted in a significant increase of RBF and in a marked fall of BP, but only little change in GFR, UF and UNaV. With a dose of 0.1 microgram/min, the parameters remained the same. Intravenous infusion of PGI2 (1 microgram/min) caused a significant fall in BP with no change in the other parameters. Both PGI2 and PGE2 had a similar effect on intrarenal hemodynamics, i.e., caused a progressively greater proportional vasodilation from superficial to deep cortex. Given intrarenally in a dose of 1 microgram/min, PGI2 and PGE2 increased renin release. But with a dose of 0.1 microgram/min and 1 microgram/min, i.v., renin secretion was not influenced. The effect of PGI2 on systemic blood pressure was more potent than that of PGE2, however, with regard to renal vasodilating action and tubular effects, PGE2 was the more potent. Present data indicate that PGI2 and PGE2 stimulate renin secretion through a direct action on the juxtaglomerular cells.

Animals↗
PubMed1980

[Studies on the thyrotropin receptor and adenylate cyclase activity in various thyroid diseases: I. The properties of TSH receptor and adenylate cyclase in Graves' thyroid and retro-orbital adipose tissues (author's transl)].

Most current etiologic concepts of Graves' disease postulate that this is an autoimmune disorder. A humoral factor, such as thyroid stimulating immunoglobulin, may be the mediator. On the other hand, it has also been suggested that abnormalities in the thyroid gland itself might be responsible for hyperfunction of the gland in Graves' disease. The true etiology of Graves' disease is still unknown. Similarly, the pathogenesis of the ophthalmic changes of Graves' disease is obscure, but immune mechanisms figure prominently in current hypotheses of the pathogenesis. It has been suggested that human adipose cell membranes have TSH receptors and that antibodies reacting with the receptors may stimulate fat cells. In this study, we have evaluated TSH receptor and adenylate cyclase of Graves' thyroid glands. Furthermore, we have investigated those of retro-orbital and the other adipose tissues in the guinea pig and in man. Human thyroid tissues were obtained at surgery and immediately minced homogenized with a loose-fitting Dounce homogenizer. A part of 10,000 g pellet of the homogenate was used for adenylate cyclase assay. The rest of the pellet was further purified by a discontinuous sucrose gradient ultracentrifugation, and the plasma membrane fraction was used for the receptor assay. The 125I-TSH binding to the fraction was measured, and the affinity constant (Ka) and capacity (Ro) were obtained from Scatchard plots using Rosenthal's method of analysis. Normal thyroid tissue contained high affinity (Ka = 2.4 x 10(10) M-1; Ro = 0.9 pmole/mg protein) and low affinity (Ka = 1.9 x 10(8) M-1; Ro = 386 pmole/mg protein) receptors. The two orders of TSH receptor were also found in Graves' thyroid tissue. The affinity constant and capacity of high affinity receptors were identical with those of normal thyroids, but the affinity constant of low affinity receptors was lower in Graves' thyroid (P less than 0.05). The basal adenylate cyclase activity in normal thyroid tissues was 0.35 nmole/10 min/mg protein. The activity rose to 280% of basal with 166 mU/ml of TSH and 680% of basal with 10 mM of NaF. These values obtained in Graves' disease were not significantly different from the values of normal thyroids. It is concluded that thyroid hyperfunction in Graves' disease is probably not the result of an intrinsic abnormality of the TSH receptor-adenylate cyclase system. Human retro-orbital adipose tissue was obtained at surgery from patients of Graves' exophthalmos or malignant neoplasm of accessory sinus. Guinea pigs tissue was obtained from 250g male animals. We were unable to demonstrate high affinity TSH receptor in human retro-orbital fat, perirenal fat or guinea pig retro-orbital fat. In contrast, guinea pig epididymal fat membranes showed TSH receptor characteristics similar to guinea pig thyroid membranes. In human adipose tissue, TSH did not stimulate the adenylate cyclase activity, although NaF definitely stimulated the enzyme...

Adenylyl Cyclases↗
PubMed1980

Effects of an orally active converting enzyme inhibitor (YS-980) on renal function in dogs.

The angiotensin converting enzyme inhibitor is a valuable pharmacological tool for studying the role of intrarenal humoral factors such as the renin-angiotensin and kallikrenin-kinin systems and prostaglandins as related to the regulation of renal function and the interrelation among them. An intrarenal infusion of such an inhibitor, YS-980 (thiazolidine carboxylic acid derivative), at a rate of 0.1 mg/min resulted in a significant fall in systemic arterial blood pressure and a significant increase in renal blood flow, urine flow, urinary excretion of sodium and renin release in anesthetized dogs. These renal effects evoked by YS-980 were abolished after the inhibition of kallikrein as induced by aprotinin (900 kallikrein inhibitory units per min). In addition, YS-980 given after the administration of indomethacin (5 mg/kg i.v.) had no effect on the renal hemodynamics and renin release except for the urinary excretion of sodium. These findings suggest that both the kallikrein-kinin system and prostaglandins contribute to the renal action and the vasodepressor effect of YS-980. It would appear that the intrarenal administration of this angiotensin converting enzyme inhibitor induced marked renal effects through activation of kinin and prostaglandins and that the relative contribution of the renin-angiotensin system is negligible in anesthetized dogs.

3-Mercaptopropionic Acid↗