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Biomedical subjects

X Xing

Publications and source records attributed to X Xing.

At least 73 records · Page 4Linked to original sources
PubMed1996

Identification of a specific DNA region required for enhanced transcription of HER2/neu in the MDA-MB453 breast cancer cell line.

Overexpression and/or amplification of the HER2/neu gene has been shown in roughly 30% of breast cancer patients. Increased levels of HER2/neu mRNA in some breast cancer cell lines is partially caused by increased gene transcription. In MDA-MB453 human breast cancer cells, an activated trans-acting factor is involved in the increased transcription of HER2/neu by mediating its effects through a specific DNA region in the HER2/neu promoter. A methylation interference experiment showed a novel sequence for protein-DNA interactions. Three polypeptides of approximately 110, 70, and 35 kD interact with this DNA element. This region of the human HER2/neu promoter is highly conserved in the rat and mouse promoters and was shown to be capable of mediating transcriptional trans-activation in HER2/neu-overexpressing MDA-MB453 cells while having little effect in a control cell line that expresses basal levels of HER2/neu. Knowledge on interactions between this DNA element and nuclear protein factors may help us better understand the molecular mechanisms regulating HER2/neu overexpression in breast cancer cells.

Animals↗
PubMed1996

[The injury of liver sinusoidal endothelial cells in a rat's isolated liver perfusion (ILP) model for regional chemotherapy].

To observe the pathological changes of liver sinusoidal endothelial cells, the regional perfusion with different dosage of 5-FU was performed by using modified rat's model of ILP. The results demonstrated that ILP with over maximum tolerence dosage of (MTD) 5-FU resulted in irreversible damage of liver and endothelial cells. The endothelial cells were more fragile than liver cells. The changes of TXA2 and PGI2 balance play an important role in the process of damage of endothelial cells.

6-Ketoprostaglandin F1 alpha↗
PubMed1996

[Normal spinal changes of bone mineral density in 445 individuals: assessment by quantitative computed tomography].

Spinal BMD was measured by QCT in 445 normal individuals aged 10-80 including 190 males and 255 females, which were divided into age-groups by every ten years. Statistic results showed that peak bone mass reached at 10-19 age-group for both male and female and that BMD assessed by QCT in females was not lower than that in males and BMD declined with increasing age. In females, BMD results in this study were different from those in USA. An acceleration bone loss was predominantly shown in the period after 40-49 age group. Among many factors related to bone loss, we consider that estrogen change may play a most important role for the remarkable bone loss in our country.

Absorptiometry, Photon↗
PubMed1996

[Predictable value of fasting blood glucose level for the incidence of non-insulin-dependent diabetes mellitus].

In order to investigate the predictable value of fasting blood glucose (FBG) level for the incidence of non-insulin-dependent diabetes mellitus (NIDDM), 638 nondiabetic subjects who were investigated in 1986 (including 341 subjects with normal glucose tolerance and 297 subjects with impaired glucose tolerance) were reexamined in 1992. The results showed that the 6-year-incidence of NIDDM was significantly increased with rising of baseline FBG level. After adjusting for age, sex and body mass index (BMI), proportional hazard regression analysis showed that FBG level in impaired glucose tolerance group was positively associated with the development of NIDDM (P = 0.0001). Subjects with mean FBG level of 5.19 mmol/L had a higher risk of developing NIDDM than subjects with mean FBG level of 4.61 mmol/L (RR 2.1, 95% CI 1.19-3.74, P = 0.01). The risk ratio of NIDDM was further increased in the group with mean FBG level of 6.l5 mmol/L (RR = 2.9, 95% CI 1.79-4.59, P = 0.0001). The result indicates that FBG level is an independent risk factor for the development of NIDDM.

Adult↗
PubMed1995

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Journal Article↗
PubMed1995

HER-2/neu-targeting gene therapy--a review.

The HER-2/neu (also named c-erbB-2) oncogene is known to be overexpressed in many human cancers, including breast, ovarian, lung, gastric and oral cancers. In animal models, HER-2/neu overexpression was shown to enhance malignancy and metastasis phenotypes. Repression of HER-2/neu overexpression suppresses the malignant phenotypes of HER-2/neu-overexpressing cancer cells, suggesting that HER-2/neu may serve as an excellent target for developing anti-cancer agents. We have previously shown that the adenovirus-5 (Ad5) E1a gene products and the SV40 large T antigen (large T) inhibit transcription of the HER-2/neu promoter and accordingly suppresses transformation induced by HER-2/neu. In this review, we summarize our recent findings on using cationic liposomes or an Ad vector to deliver E1a or large T into tumor-bearing mice. Our results indicate that both cationic liposomes or an Ad vector can efficiently deliver E1a or large T into tumor cells in mice, and this results in suppression of tumor growth and longer survival of the mice.

Adenoviridae↗
PubMed1995

The retrospective study on high risk factors of subphrenic infection after hepatectomy.

In order to identify the high risk factors that determine subphrenic infection after hepatectomy, 187 liver resections performed during January, 1985 to December, 1990 in Department of Surgery of Tongji Hospital were reviewed. Thirteen patients developed subphrenic infection with the incidence of subphrenic infection being 6.95%. The results of retrospective study on 187 liver resections demonstrated that the high risk factors related to subphrenic infection after hepatectomy were as follows: (1) The extent of liver resection was associated with subphrenic infection. (2) The incidence of subphrenic infection after liver resection of primary liver cancer patients with hepatic cirrhosis was higher than that of non-cirrhotic patients. (3) Intraoperative estimated blood loss greater than 1500 ml was found to be a significant risk factor in the development of postoperative subphrenic infection. (4) Adequate postoperative drainage of subdiaphragm and the raw surface of the liver was one of critical factors of decreasing subphrenic infection after liver resection.

Adolescent↗
PubMed1995

Reproducibility of food intake in a food frequency questionnaire used in a general population.

This study evaluates the reproducibility of food intake reports in a 45-item self-administered food frequency questionnaire (FFQ) used for a diet study in a large cohort in New York State. The first FFQ was mailed in 1980, and a followup version containing 75 food items was mailed in 1988 to the eligible original participants. Reproducibility was evaluated by comparing the subjects' 1988 responses with their original 1980 responses. Spearman's correlations ranged from 0.27 (nuts other than peanuts) to 0.56 (summer squash) in men and from 0.25 (tomatoes) to 0.51 (summer squash) in women. Analyses stratified by age, education, marital status, body mass index, cigarette smoking, alcohol drinking, and special diet revealed few substantial differences in correlations. The moderate reproducibility of foods in this questionnaire indicates that brief FFQs may be a useful tool to study food intake and chronic disease relationships in this population.

Adolescent↗
PubMed1995

[Changes and trend prediction in cancer morbidity of urban residents in Beijing].

Data from a long-term cancer morbidity registry and notification system were analyzed dynamically. A theoretical model of grey system was established based on time-series changes in cancer morbidities to predict its trend by the year of 2001 in Beijing. Results show cancer morbidities in Beijing will increase yearly from 1991 to 2001, and overall morbidity rate of malignant tumor will increase to 179.9/100,000 from 162.5/100,000. Proportions of malignant tumor in different sites will change greatly. Morbidities of lung cancer and breast cancer will increase rapidly, of liver and colon-rectal cancer shoes an increasing trend, too, of esophageal and cervical cancer will decline steadily, and of stomach cancer will begin to decline. All these facts laid a scientific basis for the study on cancer prevention and control.

Breast Neoplasms↗
PubMed1995

[Baseline hypertriglyceridemia, a risk factor for non-insulin dependent diabetes mellitus: a 6-year follow-up study of 432 nondiabetics].

The relationship between baseline fasting plasma triglycerides level (FTG) and the incidence of non-insulin-dependent diabetes mellitus (NIDDM) within 6 years were investigated in 432 Chinese nondiabetics. They were identified by oral glucose tolerance test (OGTT, oral 75 g glucose) in Daqing diabetes survey in 1986. 220 subjects were men and 212 women. Among them 226 had normal glucose tolerance and 206 impaired glucose tolerance. The measurement of fasting plasma lipids and glucose were performed at the baseline examination and after 6-year follow-up. Six-year incidence of NIDDM in the highest triglycerides tertile group (top tertile Q3, mean = 2.5 mmol/L) was 2.3 times (38.6% vs 16.6%, P < 0.01) as compared with that of the lowest triglycerides tertile group (first tertile Q1, mean = 0.7 mmol/L). The difference of 2-hour blood glucose level in OGTT (OGTT BG2h level) between Q1 and Q3 was 2.4 mmol/L (mean = 7.6-10.0 mmol/L) after 6 years. Multivariate general linear regression analysis demonstrated that the FTG level was positively associated with the OGTT BG2h level after 6 years (P = 0.0335) after controlling the age, sex, fasting blood glucose (FBG) and body mass index (BMI). Therefore this result indicates that FTG level is an independent risk factor for the development of NIDDM in this population.

Adult↗
PubMed1995

[Changes in surface microcirculation of extensor digitorum longus tendon after ischemia-reperfusion of rabbit hindlimbs].

The circulation was stopped by tourniquet in one rabbit hindlimb. After 2 or 5 hr of ischemia, the hindlimb was reperfused. During the first 1 hr of reperfusion, dynamic changes in surface microcirculation of extensor digitorum longus tendon were observed by using intravital microscopy. Particular attention was paid to leukocyte-endothelium adhesion and microvascular perfusion condition. It was found that 1) the number of leukocytes adherent to the venular endothelium of ischemic tissue significantly increased following reperfusion and the degree of increases appeared to be directly related to the duration of ischemic period; 2) reperfusion after 5 hr of ischemia could result in the formation of "no reflow phenomenon" in the ischemic tissues, including "primary no reflow" and "secondary capillary perfusion failure". These results suggest that 1) leukocyte-endothelium adhesion may contribute to the development of ischemia reperfusion injury; 2) in the limbs undergone 5 hr of ischemia, not all injuries that occur after ischemi-reperfusion can be attributed to ischemia reperfusion injury. The injury seen some in areas may be produced by ischemia alone, and in others ischemia-reperfusion-secondary ischemia injury may be responsible.

Animals↗
PubMed1995

[Clinical application of immediate intraoperative expansion technique].

Since June 1992, 14 patients with skin or soft tissue defects from resection of scar or nevus were treated using the technique of immediate intraoperative expansion with good results. The operative procedure, indications and key points are discussed. We concluded that immediate intraoperative expansion may be accepted as a simple, safe and effective technique for the repair of the skin defects, which can be sutured directly under great tension.

Adolescent↗