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Biomedical subjects

X Wu

Publications and source records attributed to X Wu.

At least 505 records · Page 28Linked to original sources
PubMed1999

[Study on the contents of serum HDL subclasses in type N hyperlipidemics].

Contents of serum HDL subclasses in normolipidemics (n = 24) and type IV hyperlipidemics (n = 24) were determined using two-dimensional gel electrophoresis associated with immunodetection method. The results showed that the contents of pre-beta 1 HDL (P < 0.001) and HDL3a (P < 0.05) increased significantly and that of HDL2b (P < 0.001) decreased significantly in hyperlipidemics, compared with normolipidemics. Serum TG concentrations of hyperlipidemics showed positive correlation with pre-beta 1 HDL (r = 0.582) and HDL3a (r = 0.692) and negative correlation with HDL2b (r = -0.506) and HDL2a (r = -0.552). These results indicated that the maturation of HDL could be abnormal in type IV hyperlipidemics.

Adult↗
PubMed1999

[The changes of GFAP, PCNA after brain contusion: an immunohistochemical study in forensic pathology].

After the model of experimental brain contusion was set up, the changes of GFAP, PCNA were studied by immunohistochemistry SP method combined with image quantity analysis. After contusion, the gray degrees and areas of GFAP-positive cells increased significantly at the 3rd hour, reached the highest level in the 4th day and kept until the 7th day. PCNA-positive cells appeared in the 12th hour, simultaneously, their gray degrees tended to increase and positive areas tended to decrease. It showed regular changes of GFAP, PCNA with various survival times, and these changes were useful in the diagnosis of brain contusion especially in the period from the second to 7th day after injury. The numbers of astrocytes increased and reached the highest level in the 3rd day after contusion while the reactive proliferation of astrocytes is only a minor phenomenon.

Animals↗
PubMed1999

[Cloning and sequencing of variable region gene of heavy chain of monoclonal antibody against SA I/II of Streptococcus mutans].

OBJECTIVE: To clone and sequence a immunoglobulin variable region of heavy chain (VH) from a mouse hybridoma 2B12F6, which produce monoclonal antibody against SA I/II of Streptococcus mutans. METHODS: The immunoglobulin variable region gene of heavy chain of 2B12F6 was amplified and cloned into pUC18 by using PCR technique and gene engineering technique, and then the gene sequence was analyzed by Sanger's method. RESULTS: The VH gene segment was 360 base pairs in length and coded 118 amino acids, and the homology of framework of VH gene and mouse VH gene published was 70%, which accorded with the feature of mouse VH gene. CONCLUSION: The VH gene gained from 2B12F6 could provide the possibility of construction of gene engineering antibody against SA I/II of Streptococcus mutans.

Amino Acid Sequence↗
PubMed1999

[The immunohistochemistry and clinic study on surgical margin of laryngeal cancer].

OBJECTIVE: To probe into the safe incisal range of removing laryngectomy cancer determined from the albumen balance of cells. METHOD: LSAB or SABC of immunohistochemistry was applied, and three groups of samples with cancer tissue, nearby cancer tissue and distal cancer tissue of 36 primary laryngectomy cancer were tested by antibodies p16, p21, TGF-beta 1, PCNA and EGFR. RESULT: The difference of the positive rate with the five antibodies between the cancer group and the two side incisal group were very notable with P < 0.01 or P < 0.05, but which between nearby cancer tissue and distal cancer tissue were not notable with P > 0.05. The differences of the positive rates with 5 antibodied between high differentiation group and low differentiation group were notable with P < 0.05, and P < 0.05 with PCNA in the group whether the lymphonodi cervicales transmitted or not. The incisals for all the cases are beyond 5 mm, the recurerence rate is 3%. CONCLUSION: The 5 mm incisal for laryngectomy cancer is appropriate on the albumen cevel of cells.

Adult↗
PubMed1999

[An electrochemical cell for end-column amperometric detection in capillary electrophoresis].

With end-column electrochemical detection in capillary electrophoresis(CE), precise positioning and stabilization of the working electrode are very important. In this paper, an electrochemical cell designed for end-colunm wall-jet amperometric detection in capillary zone electrophoresis is reported. In this cell, a curved surface was formed because of the surface tension and used as the amplifier. With this cell, alignment of the working electrode with the capillary outlet could be achieved precisely and easily without the help of a microscope and a micropositioner. Being compared with electrochemical cell of other groups, this cell is simple and can be used more conveniently. Application of this electrochemical cell for electrophoretic separation of phenols is demonstrated. High resolution and low detection limits were obtained with this assembly.

English Abstract↗
PubMed1999

[Computer-aided teaching system of heart sound].

This paper details a full set of plan of the computer-aided teaching of heart sound. By means of digital sound technology one is capable of playing realistically heart sound of various kinds based on database. Each heart sound is demonstrated by a proper explanation of its characteristics and a heart sound graph. This method gets over the shortages of traditional teaching methods. Besides, this system can be used to test users' level of auscultation.

Computer Systems↗
PubMed1999

[Development of continuous rat infusion device].

In order to perform better scientific researches on fluid resuscitation, total parenteral nutrition and continuous drug administration in rats, we developed a set of rat infusion device. The device, the characteristics of reasonable design, simple operation, low cost, good practicability, and making it by oneself or buying a whole set, can not only assure rats to run around, drink water, eat food freely, but also insure continuous infusion and drug administration during the experimental study of rats.

Animals↗
PubMed1999

[Expression of Schistosoma japonicun fatty acid binding protein gene in silkworm cells and larvae].

AIM: To express the fatty acid binding protein (Sj14FABP) gene of Schistosoma japonicun in the silkworm cells and larvae. METHODS: A 600 bp DNA fragment containing Sj14FABP gene was cloned into baculovirus transfer vector of pBacPAK His1 to construct recombinant transfer vector Sj14-pBac PAK His1. Coinfection was accomplished with this vector and Bombyx mori nuclear polyhedrosis virus (BmNPV) DNA in BmN cells. The recombinant virus of Bm-Sj14 was screened using dot-blotting. The BmN cells and silkworm larvae were infected with Bm-Sj14 to express Sj14FABF gene. Western blotting and ELISA were used to identify the antigenicity of the recombinant protein. RESULTS: Sj14FABP gene was successfully expressed in the BmN cells and silkworm larvae infected with Bm-Sj14. The product was a 18 kDa fusion protein. The yield in BmN cells was about 100 micrograms/1 x 10(6) cells and 33 micrograms/ml cell supernatant. In silkworm larvae, the product yield was 4 mg/ml haemolymph as well as 4.6 mg/g silkworm tissue. The recombinant protein could be recognized by Western blotting and ELISA using the sera from mice immunized with SWAP. CONCLUSION: Sj14FABP gene has been successfully expressed in BmNPV system and the product has high antigenicity.

Animals↗
PubMed1999

[Effects of growth factors and estrogen on the proliferation and prolactin gene expression in anterior pituitary cells of rats].

OBJECTIVE: Detect the effects of exogenous 17 beta-estradiol (E2), epidermal growth factor (EGF), and transforming growth factor beta 1 (TGF beta 1) on the proliferation and prolactin (PRL) gene expression in primary serum-free cultured anterior pituitary cells in vitro. METHODS: Laser scanning confocal microscopy (LSCM) and in situ hybridization in primary serum-free cultures of rat anterior pituitary cells were employed. RESULTS: After 36 hours incubation of the monolayer with E2(10(-8) mol/L) and EGF(10(-8) mol/L), DNA and PRL mRNA contents in the cells were increased significantly (P < 0.001); and when cells were co-incubated with E2 and EGF at the same time, the levels of DNA and PRL mRNA were higher than those treated with E2 or EGF alone (P < 0.01), respectively. TGF beta 1(2 ng/ml) treatment decreased the DNA and PRL mRNA contents significantly (P < 0.001). Its inhibitory effect was reduced at the presence of E2, the DNA and PRL mRNA levels in the cells were higher than those treated with TGF beta 1 alone (P < 0.001), but still lower than E2 alone treatment (P < 0.001). CONCLUSIONS: The results indicate that EGF and TGF beta 1 exerte stimulatory inhibitory effects, on cell proliferation and PRL gene expression in anterior pituitary cells of rats in both basal and E2-induced conditions. EGF and TGF beta 1 may be involoved in the regulation of proliferation and PRL gene expression in anterior pituitary cells in vivo; and also may be correlated with prolactin-secreting tumors formation induced by E2.

Animals↗
PubMed1999

[Clinic observation on the prothetic results of dental supply implant system].

OBJECTIVE: To make a clinical evaluation by the Dental Supply implant system by three years follow-up study. METHODS: 46 patients treated by Dental Supply implant system were chosen for three years follow-up investigations. RESULTS: The ball-bar framework complete denture was the best and the prostheses for single molars were almost a failure, as the success rate was only 14.3%. CONCLUSIONS: Dental Supply system was not appropriate to restore the single tooth and posterial fixed bridge.

Adult↗
PubMed1999

[Study on the inclusion effect of menthol with beta-cyclodextrin].

Indusion effect of beta-CD with menthol was studied by three methods of preparation. Determination of physical constants and quantitative analysis proved that the inclusion compounds of beta-CD with menthol were formed, which was found to be 1:1 and 2:3. The results of thermal analysis demmonstrated that the gasifiation temperature of the inclusion compounds was by far higher than that of the free menthol. It was considered that the inclusion compound must be disconnected to be free, and then it can be determined by gas chromatography in the same condition of the free menthol.

Chromatography, Gas↗
PubMed1999

Differentiation of embryonic stem cells into neurons and retina-like structure in nude mice.

PURPOSE: To investigate the intraocular growth and biological characteristics of mice embryonic stem cells in nude mice. METHODS: Murine embryonic stem cells (D3 cell line) were cultured and maintained in an undifferentiated state in vitro, then transplanted into the anterior chamber of nude mice. Mophological and immunohistochemical examinations were implemented. RESULTS: Two to three days after transplantation, yellow-white floating granules, sheets and masses were seen inside the anterior chamber and vitreous cavity, and enlarged gradually. 14-20 days later, the mice were executed. Morphological examination showed that there were undifferentiated cells and some round or polygonal differentiated cells in anterior chamber and vitreous cavity. The morphology of these differentiated cells were similar to that of the retina. The cells were highly positive in NSE staining. CONCLUSION: The transplanted embryonic stem cells could grow in the eyes of nude mice with tendency to differentiate into neurons and retina-like structure.

Animals↗
PubMed1999

[Study on the action force of dimerization of cobalt tetrasulfonate Ph-thalocyanine in aqueous solotion].

The dimerization of cobalt tetrasulfonate phthalocyanine (CoTSPc) was studied in the microemulsion, micelles and aqueous alcoholic solutions. The coordination reaction for the formation of complexes CoTSPc (L), which was formed by cobalt tetrasulfonate phthalocyanine (CoTSPc) plus ligand L(L = NH3, CN-, en), was also studied. The reaction kinetics was discussed and the stability constant K of CoTSPc(L)2 was also calculated from absorbance measurement.

English Abstract↗
PubMed1999

[Simultaneous determination of molybdenum and chromium by artificial neural networks spectrophotometry].

In this paper, a three-layer artificial neural networks were applied to simultaneous determination of molybdenum and chromium by spectrophotometry. The apparent molar absorptivities of the complex of molybdenum and chromium are 1.4 x 10(5) L x mol(-1) x cm(-1) and 3.7 x 10(4) L x mol(-1) x cm(-1), respectively. A method has been developed for simultaneous determination of molybdenum and chromium in steel with satisfactory results. The paralysis in the procedure of training about ANN has been avoided with the improved back propagation algorithms. The simple transformation method for target vectors and criterion function convenient for selection of the parameters about the networks has been put foward.

English Abstract↗