Biomedical subjects

X Liu

Publications and source records attributed to X Liu.

At least 685 records · Page 38Linked to original sources

PubMed2000

[Factor analysis on the intelligence of patients with schizophrenia].

The authors made a factor analysis on the intelligence of patients with schizophrenia. 119 patients with schizophrenia were measured by Wechsler Adult Intelligence Scale-Revised China (WAIS-RC). The results revealed the average full intelligence quotient (IQ) of schizophrenia was 79.70, the verbal intelligence quotient(VIQ) 84.43, and the performance intelligence quotient(PIQ) 74.10. Multiple regression showed the regression coefficient of educational level for IQ was 8.6721. The regression coefficient of the course of disease for IQ was negative 0.1519. Factor analysis showed that the cumulative percent of three-factor solution of schizophrenia was 73.5. In conclusion, the IQ of most patients with schizophrenia was lower than norm, VIQ > PIQ(P = 0.0000). Educational level had greater influence on IQ. The longer the course of disease was, the worse the intelligence came to be. Factor analysis indicated that three-factor solution of schizophrenia was similar to that of norm, but each subtest value was lower than norm.

Adult↗

PubMed2000

[Isolation and phenotypic analysis of lamina propria mononuclear cells from colonoscopic biopsy specimens].

This study was directed to the method of isolation of lamina propria mononuclear cells (LPMC) from mucosal biopsy specimens and the phenotypic analysis of the cells. Ten biopsy specimens, taken individually from 8 patients with ulcerative colitis (UC), 5 normal controls and 22 patients with colorectal cancer, were collected to compare the factors that influence isolation and cell yield and to analyze the lymphocyte subsets by means of two-color flow cytometry. LPMC yield averaged 10(6) with a viability of more than 95% in UC, twice the yields in other two groups. The percentages of total T, B cells in three groups were similar (P > 0.05). When the UC group was compared with the other two groups, a significantly higher proportion of CD3(+)-CD4(+)-T cell (49.98% vs 37.54% and 37.25%, P < 0.01) was noted, whereas a lower proportion of CD3(+)-CD8(+)-T cell (23.64% vs 31.52% and 31.07%, P < 0.01) was observed. These data showed that the yield and viability of LPMC isolated from biopsy specimens were good enough for a phenotypic or functional analysis, which might be helpful to mucosal immune research on gut disorders, such as UC.

Biopsy↗

PubMed2000

[Inhibition of the activation and collagen production of cultured rat hepatic stellate cells by antisense oligonucleotides against transforming growth factor-beta 1 is enhanced by cationic liposome delivery].

In order to investigate the inhibition of the activation and collagen production of cultured rat hepatic stellate cells (HSC) by antisense oligonucleotides (ASON) against TGF beta 1 after cationic liposome (lipofectin) delivery, the authors synthesized a 20-mer phosphorothioate antisense oligonucleotide of TGF beta 1 mRNA, and its sense of missense oligonucleotides, and then treated the HSC with cationic liposome/oligonucleotide complexes respectively. The cellular uptake of 32P-labelled oligonucleotides was determined by liquid scintillation counting, and HSC activation was assessed by the expression of alpha-smooth muscle actin(alpha-SMA). The cellular uptake of lipofectin/32P-ASON complex was approximately five-fold higher than that of 32P-ASON alone. Cationic liposome (lipofectin) delivery significantly increased the inhibition of HSCs activation by ASON, while compared with the use of naked TGF beta 1 ASON at the same concentration (at a final concentration of 1 mumol/L, P < 0.05). However, these effects were not observed in lipofectin alone, liposome/sense or missense oligos complexes at the same concentration. The oligonucleotide or lipofectin/oligos complexes had no cytotoxicity to rat HSCs in culture. These findings suggest that cationic liposome is an effective vehicle to improve the delivery of ASON to rat HSCs in culture, and the cationic liposome/TGF beta 1 ASON complex may be useful for the treatment of hepatic fibrosis.

Actins↗

PubMed2000

[An experimental study combined on traditional Chinese medicine with immunosuppressant for treatment and prevention of tubular interstitial nephritis].

The objective of this study was to determine the effect of Chinese herbs and immune inhibitors on diffuse proliferative glomerulonephritis. The tubular-interstitial infiltration of cell, TGF-beta expression and interstitial fibrosis were investigated, and the relationship between clinical data and pathological changes was analysed. The results showed the infiltration of cells was inhibited in the "Chinese herbs combined with prednisone" group, the infiltration of cells, TGF-beta expression and interstitial fibrosis were all inhibited in the cyclophosphamide and prednisone" group. But in the prednisone group, interstitial fibrosis was not inhibited. These data suggest that the combined use of Chinese herbs, immunosuppressant and prednisone can inhibit the interstitial cell infiltration and prevent the interstitial fibrosis in diffuse proliferative glomerulonephritis.

Animals↗

PubMed2000

[Response to milrinone treatment in patients with chronic congestive heart failure].

The objective of this study was to examine the efficacy of milrinone in chronic congestive heart failure. In this randomized, double-blind, placebo-controlled trial, 30 patients with class III or IV chronic congestive heart failure were randomly assigned to receive milrinone (n = 15) or placebo (n = 15). The results showed that in the milrinone group, stroke volume increased from 38.50 +/- 5.34 to 64.93 +/- 6.46 ml (P < 0.001), cardiac output increased from 3.39 +/- 0.42 to 5.28 +/- 0.55 L/min (P < 0.001), cardiac index increased from 2.01 +/- 0.42 to 3.21 +/- 0.57/min/m2 (P < 0.001) and ejection fraction increased from 0.23 +/- 0.05 to 0.35 +/- 0.06(P < 0.001). In the placebo group, no hemodynamic effects were found. Milrinone favourably influenced the symptoms and heart function in 11/15(73.3%) patients. In the placebo group, only 4/15(26.7%) patients had such response (P < 0.05). These data indicate that milrinone is effective for improving the hemodynamics status and heart function in patients with chronic heart failure.

Adolescent↗

PubMed2000

[Analysis on skin allergy of rural residents in Anqing area].

To investigate the allergy reaction of rural residents against 12 categories sensibiligen, 9,232 sensibiligen peasants from rural areas of Anqing were observed by 12 categories sensibiligen in skin(methods: point thron). If the diameter of papule erygthema was bigger than that of histamines of control, the reaction was considered to be positive. All cata was analyzed by sas software. The results showed that the allergy reaction degrees were different among the groups with different genders and ages. Family susceptibility was noticed.

China↗

PubMed2000

[Determination of guanine plus cytosine content of Pseudomonas cocovenenans subsp farinofermantans with two methods and the comparative study].

The guanine plus cytosine content (G + C) mol% of Pseudomonas cocovenenans subsp farinofermantans, Burkholderia gladioli and Burkholderia cocovenenans, which were similar in biochemical characteristics, were determined with Tm values method and HPLC method, and the comparative study of these two methods was discussed. A negative conclusion could not be made from the results. The three bacteria were the same species. The HPLC method was more accurate than the Tm values method, but the repeatability of the latter was better.

Bacterial Typing Techniques↗

PubMed2000

[Application of indigo disulphonate spectrophotometry on the determination of ozone in indoor air].

Spectrophotometric determination of ozone in indoor air by using indigo disulphonate was investigated. The results showed that the average recovery of ozone was 99% and the relative standard deviation was 2.3% when the concentration of indoor ozone was in the range of 0.2-1.0 microgram/ml. The lowest detection concentration was 0.009 mg/m3 (sampling 20 L) when the range of determination was 0.18-10.0 mg/10 ml.

Air Pollutants↗

PubMed2000

[Determination of trace elements in serum of tuberculosis patients].

In order to investigate the changes of molybdenum (Mo), zinc (Zn), copper (Cu), manganese (Mn) and seleniumin (Se) level in serum of tuberculosis patients, the concentrations of Mo, Zn, Cu, Mn and Zn in serum of 30 tuberculosis patients and 30 health controls were determined by inductively coupled plasma mass spetrometry. The results showed that compared with the control group, the levels of Mo, Zn, Cu, and Se in the serum of tuberculosis patients decreased significantly (P < 0.001), but the change of Mn was insignificant (P > 0.05).

Adolescent↗

PubMed2000

[Analysis on forensic medical appraisals and VEP characteristics of ocular trauma caused by traffic accidents].

In order to investigate the characteristics of forensic medical appraisals of ocular trauma caused by traffic accidents, Visual Evoked Potential examinations were performed in 30 cases and the results were analyzed. It was found that, compared with 30 cases of ocular trauma caused by ordinary injuries, (1) the proportion of craniocerebral injuries (83%) and cranial nerve injuries (53%) was much higher, so were the proportion of diplopia (30%), optic never injuries (20%), and orbital wall fracture (20%). (2) because victims always claimed that their visual acuity was reduced by traffic accidents, it was difficult to identify simulated lower visual acuity by ordinary method, and VEP examination was often used. Different degrees of visual functional disorders showed different patterns in VEP, such as no VEP wave, lowed P1 wave amplitude, delayed P1 period, and so on.

Accidents, Traffic↗

PubMed2000

[Establishment of rat submandibular gland squamous cell carcinoma induced by DMBA].

OBJECTIVE: To study carcinogenesis and development of salivary gland tumor and establish an animal model of submandibular gland (SMG) tumor. METHODS: Histopathological study during carcinogenesis in rat SMGs using (9,10-dimethyl-1,2-benzanthracene (DMBA) was evaluated. A total of 50 male and female Sprague-Dawley (SD) rats of 8 weeks old and 180-200 g weight were obtained from the Animal Center of Henan Medical University. Under pentobarbital sodium anesthesia, the left SMGs were exposed by surgical procedure. A sponge pellet (1.0 mm x 1.0 mm x 1.0 mm, made by authors) was used as the carrier of the carcinogen. The sponge containing 2% DMBA (Fluka, Switzerland)/acetone solution was implanted into the glandular tissue of the left SMGs. Four rats (2 males and 2 females) were killed after every 2 weeks of the DMBA/sponge implantation. The same method of sponge implantation without DMBA was used at the right side of SMG as a control. All rats left were killed after 20 weeks. The SMGs were fixed in 10% formalin buffer solution for 24 hours, and embedded in paraffin, then 4 microns-thick sections were made for histopathological study. RESULTS: The earliest tumor occurred after 4 weeks of implantation of sponge, a total of 21 lateral tumors were induced (10 females, 11 males). There was no tumor found in the controlled SMG. The peak time of tumor genesis was after 8-12 weeks of implantation, all tumors induced were squamous cell carcinomas(SCCs). The induced tumors grew slowly below the mandibles of rats, which were present as nodular masses without capsules, and the borders were not clear. They were slightly hard when palpated. The process of carcinogenesis can be described as following: squamous metaplasia of cyst-like structures occurred, then SCCs were induced and invaded surrounding tissues. No metastasis was observed in regional lymph nodes and other organs. CONCLUSION: SCCs of SMG can be induced by implantation of DMBA. The present study supports the conclusion that all duct segments undergo squamous metaplasia, and therefore may participate in the genesis of neoplasia during experimental carcinogenesis.

9,10-Dimethyl-1,2-benzanthracene↗

PubMed2000

[Chiral separation of glycerin monosulfides and bisulfides with a stationary phase of amylose-tris(3,5-dimethylphenylcarbamate)].

Amylose-tris(3,5-dimethylphenylcarbamate) (ADMPC) and aminopropylated silica gel(APS) were prepared after the reported methods. ADMPC was immobilized on APS from tetrahydrofuran solution with a coating amount of 15%. The chiral stationary phase was packed in a stainless-steel column(150 mm x 4.6 mm i.d.) by slurry method. The column was used for the enantioseparation of 6 glycerin monosulfides and 4 glycerin bisulfides. Mixtures of hexane and 2-propanol were used as mobile phases. The enantiomers of the monosulfides could be separated quite well, while those of the bisulfides could not at all. This phenomenon shows that the ether oxygen atom of the monosulfides plays a key role in the chiral discrimination process. The retention time of the solutes increases significantly as the amount of 2-propanol decreases. This shows the main interaction between the solutes and the chiral stationary phase is hydrogen bonding. A dynamic model is presented to account for the chiral discrimination mechanism.

Amylose↗

PubMed2000

[Value of intestinal decontamination by traditional Chinese medicine-X in the prevention of bacterial translocation complicated by severe acute pancreatitis in rats].

This study was designed to evaluate the efficacy of traditional Chinese Medicine-X in preventing the necrotic infection of the pancreas in severe acute pancreatitis (SAP). Sixty rats were randomly divided into five groups with 12 rats in each one: (1) normal control, (2) SAP + 0.9% normal saline (1 ml x 100 g-1 x 24 h-1), (3) SAP + gentamycin (2000 u x 100 g-1 x 24 h-1), (4) SAP + TCM-X (1.0 g x 100 g-1 x 24 h-1), and (5) SAP + gentamycin (2000 u x 100 g-1 x 24 h-1) + TCM-X (1.0 g x 100 g-1 x 24 h-1). The medicines were given by way of gastrotube, once every 24 hours, twice in all. Pancreatitis was induced by a single intraperitoneal injection of 500 mg.100 g-1 of L-arginine. Serum endotoxin were observed and the clone forming units from mesenteric lymphnode and pancreas were obtained after 48 hours treatment. 96 hours after the experiment, the bacteria found in the mesenteric lymphnodes and pancreas in groups three, four and five were reduced as compared to that in group two; the levels of serum endotoxin were reduced, too. These data indicate that TCM-X and gentamycin in decontamination by way of gastrotube are effective in preventing bacterial translocation complicated by SAP, and the effect of TCM-X is stronger than that of gentamycin.

Acute Disease↗

PubMed2000

[Molecular cloning of enhancin gene from Helicoverpa armigera granulosis virus and its expression in E. coli].

In order to provide recombinant enhancin for studying its mechanism of increasing the mortality of larvae infected by HaNPV and creating new insecticide, enhancin gene from Helicoverpa armigera granulosis virus was amplified by PCR technique. 2.7 kb fragment of enhancin gene was cloned into EcoRI/XbaI site of plasmid pBluescript KS. Sequence analysis revealed that enhancin gene was similar with that reported in the literature except eight nucleotides and five amino acids. Thereby enhancin gene was inserted into vector pET-30a and expressed successfully in Escherichia coli BL21(DE3). The preliminary bioassay of expressed product and HaNPV indicated that mortality of larvae increased 31.7%-34.1% in 7 post-infection days and the LT50 decreased at least 1.5-2.1 days.

Amino Acid Sequence↗

PubMed2000

[Cloning and expression of Shiga-like toxin type II variant B gene of E. coli].

A structure sequence and a DNA fragment including the signal peptide sequence and structure sequence of Shiga-like toxin II variant B subunit gene were amplified from E. coli strain O138 by PCR. After digested with restriction endonuclease EcoRI and BamHI, the two genes were orientally inserted into the polycloning site of expression vector pYA3334 (asd+) respectively. Recombinant plasmids pB0 and pB1 were constructed and amplified in E. coli X6212 (asd-). pB0 and pB1 were then introduced into avirulent Salmonella typhimurium vaccine strain X4550 (asd-) by serial transformation through intermediate strain X3730 (asd-) to construct recombinant SLT-IIvB strain. Results of nucleotide sequencing of the cloned fragments in pB0 and pB1 revealed that they were in correct ORF of SLT-IIvB. The results of SDS-PAGE and Western-blot showed that 7.6 kD protein of SLT-IIvB antigen was expressed at pretty high level in recombinant strain X4550(pB0). The results of mice immunization indicated X4550(pB0) could initiate the host to produce specific antibodies to SLT-IIvB and LPS-O antigen of X4550. So the recombinant strain X4550 (pB0) is worth considering as a candidate vaccine strain against porcine edema disease and Salmonella typhimurium infections.

Animals↗

PubMed2000

[Study on the mechanism of hemocompatibility of titanium oxide films].

In this paper a study on the facts affecting blood compatibility of titanium oxide films synthesized by ion beam assisted deposition is presented, and the mechanism of blood compatibility is discussed. We think that the blood compatibility is determined by surface energy and work function of the film. The protein adsorption is determined by surface energy. An area with a range of values of polar and dispersion components of surface energies, where good protein adsorption property will exhibit, was defined. That is, more human serum albumin (HSA) and less human fibrinogen (HFG) will be adsorbed in this area. Work function is also an important factor to influence blood compatibility. Low work function can inhibit the decomposition of HFG and improve thromboresistance.

Adsorption↗

PubMed2000

[Study on the mechanism of pulmonary edema after seawater drowning in rabbit].

To study the mechanism of pulmonary edema after seawater drowning (PE-SWD), the indexes of blood-gas and acid-base in rabbits artery blood were measured by the blood-gas analyser. The activity of Na(+)-K(+)-ATPase, cytochrome oxidase(CYTO) and alkaline phospharase(ALP) in the lungs were measured and analysed by computer image system. C-fos mRNA and Fos protein in the lungs were respectively determined by situ hybrioization and immunohisto chemical techniques. The distribution of phospholipid and Ca2+ of rabbits lungs was quantitatively analysed by ultrastructural location method. The results showed that, five parameters of PaO2, oxygen saturation(SaO2), pH, actual bicarbonite(AB) and base excess(BE) and the activity of Na(+)-K(+)-ATPase and CYTO decreased remarkably in PE-SWD. Both c-fos mRNA and Fos protein expression in pulmonary epithelial cells in PE-SWD were significantly elevated compared with the normal controls(P < 0.01). The phospholipids products in the pulmonary alveolar type II epithelial cells were decreased, however, the Ca2+ precipitate pellets inside the lung capillary endothelial cells and the pulmonary alveolar type I and II epithelial cells increased obviously. The arthors suggest that the injuny action of the seawater, hypoxia and metabolic acidosis may be the mian three mechanisms of the pulmonary edema induced seawater drowning. The lowering activity of Na(+)-K(+)-ATPase and CYTO in the lungs and calcium overload in the cells are not only evil consequence resulted from above three factors, but also the importent causes leading to the worse of PE-SWD. The transmiting line of Ca(2+)-fos may be also a key link to bring about the worse of PE-SWD.

Acidosis↗

PubMed2000

[Bacteriological study of single nasal sinusitis and sensitivity of antibiotics].

OBJECTIVE: To properly understand the bacterial distribution and susceptibility to antibiotics in the sinus of nasal sinusitis. METHOD: The mucosal and secretion in sinus were taken from 135 patients who suffered with single nasal sinusitis, by functional endoscopic sinus surgery, were cultured for bacteria. Meanwhile the antimicrobial susceptibility was determined for ordinary antibiotics. RESULT: The bacterial growth was present in 88.15% of cases, mixed bacterial growth was present in 52.10% of all bacterial, anaerobe growth was present in 39.06% of all bacteria, the susceptibility to penicillin was in 53.12% of all bacteria and cefaolin was in 87.50%. The susceptibility to metronidazole was in 94.67% of all anaerobes. CONCLUSION: We think the method that penicillin in combination with streptomycin and metronidazole, sufameth oxazole united with metronidazole, cefaolin added metronidazole were effective in nasal sinusitis treatment before the antimicrobial susceptive examination had been done.

Anti-Bacterial Agents↗