Search PubMed⌕ Search

Biomedical subjects

X Jiang

Publications and source records attributed to X Jiang.

At least 451 records · Page 25Linked to original sources
PubMed1993

[Functional and pathohistologic changes in an ischemic model of rat inner ear].

We investigated functional and histopathological changes in the inner ear circulatory disorder caused by thrombosis of the anterior inferior cerebellar artery (AICA) in rats. The thrombosis was induced by a photochemical reaction between green light and systemic injection of Rose Bengal. During this experiment, auditory brainstem responses (ABR) were recorded and cochlear blood flow (CBF) was measured by a laser doppler flowmeter. Within 5 weeks after the procedure, histopathological changes were studied by light microscopy following the observation of equilibrium dysfunction and ABR recording. CBF was reduced from the baseline in 30.8 +/- 3.4% (mean +/- SE, n = 25) by occlusion of the AICA, ABR was altered in 96% of animals, and equilibrium dysfunction was observed in 77%. When the AICA was not reopened within a short time, severe histopathological changes were frequently observed. There was no significant correlation between equilibrium dysfunction and histopathological changes in the vestibule. It is concluded that this experimental model may be useful for investigating functional and histopathological changes in the ischemic inner ear.

Animals↗
PubMed1993

[Determination of glycyrrhizin in glycyrrhiza and it's preparations by ion-pair HPLC].

Glycyrrhizin is the main component of glycyrrhiza. In this article an Ion-pair high performance liquid chromatography (HPLC) method is described for the determination of glycyrrhizin in two kinds of glycyrrhiza from different origins and twelve kinds of Chinese traditional patent medicine containing glycyrrhiza. A reversed phase system was used, including an ODS column with water and methanol (36:64) as the mobile phase which contained 5mmol/L tetra-n-butyl ammonium hydroxide and was adjusted to pH 6.0 by phosphoric acid. The column temperature was 35 degrees C; the detection was performed at 254 nm. Under this condition glycyrrhizin could be separated from other components. The limit of detection was 13ng, and the average recovery was 99.85%. During the experiments, we studied the concentration of counter-ion, the mixing ratio of methanol to water, the pH of the mobile phase and column temperature and their effect on the capacity factor of compound and resolution. We also studied the method of pre-treatment for the samples. The determination method is simple and accurate.

Chromatography, High Pressure Liquid↗
PubMed1993

The Vin-1 gene, identified by provirus insertional mutagenesis, is the cyclin D2.

The Vin-1 gene was initially identified as a gene whose expression is altered by the integration of proviruses in the Vin-1 common site of integration in retrovirus-induced rodent T-cell leukemias. We have now isolated the Vin-1 cDNA. Sequencing of the Vin-1 cDNA and Vin-1 exons revealed that the proviruses are integrated at the 5' end of the Vin-1 gene in an inverse transcriptional orientation. The sequence of the Vin-1 gene is identical to that of the recently identified G1-phase cyclin D2 gene. The human homolog of the Vin-1/cyclin D2 gene (CCND2) was mapped to chromosome 12, band p13.3, by in situ hybridization, confirming previous mapping data. Our results strongly support a role of the cyclin D2 gene in oncogenesis and thereby implicate altered cell cycle regulation in transformation.

Amino Acid Sequence↗
PubMed1992

The effect of lipid peroxides and superoxide dismutase on systemic lupus erythematosus: a preliminary study.

Serum lipid peroxide (LPO) levels, superoxide dismutase (SOD) activity in peripheral blood erythrocytes, serum ANA, anti-dsDNA, and C3 were measured in 83 patients with systemic lupus erythematosus (SLE) and in 29 healthy controls. Compared to the controls, LPO levels were significantly increased in active SLE patients while SOD activity was markedly decreased. From the active phase to the inactive phase, a gradual decline in LPO levels and an increase in SOD activity were observed. There was a close correlation between LPO levels and disease activity or the parameters including ANA titers, anti-DNA titers, and C3 levels; a significant negative correlation was found between SOD activity and LPO levels or these parameters. Further, a marked difference in SOD activity was found in untreated active cases with and without nephritis. As the SLE patients improved, LPO levels gradually declined and SOD activity increased. The present study indicates that free radicals and resultantly formed lipid peroxide levels are higher in patients with SLE than those in normal persons, probably contributing to the production of autoantibodies, nephritis, and vasculitis of other organs, and that excessively generated free radicals may play an important role in the pathogenesis of SLE, which is possibly due to diminished SOD activity.

Adolescent↗
PubMed1992

Building protein backbones from C alpha coordinates.

An automatic procedure for building polyalanine backbones from guiding alpha-carbon positions is presented. Polyalanine backbones are built based on the geometric restraints of angle N-C alpha-C and the knowledge of main-chain dihedral angle distributions. A building module constructs a list of polyalanine backbones that follow exactly the C alpha trace. Then a selection module selects one backbone with the largest portion of phi-psi pairs in favoured regions. Several test cases on C alpha coordinates from X-ray refined structures give acceptable results. Less than 10% of the peptide planes are incorrectly built, and the result is not sensitive to random shift up to 0.5 A of C alpha coordinates.

Amino Acid Sequence↗
PubMed1992

Detection of Norwalk virus in stool by polymerase chain reaction.

A method of reverse transcription (RT) and polymerase chain reaction (PCR) for the detection of Norwalk virus in human stools was developed. A cationic detergent, cetyltrimethylammonium bromide, was found to effectively remove from stool extracts factors that inhibit the RT-PCR assay. The specificities of the tests were shown by hybridization of the amplified DNA with Norwalk virus-specific cDNA probes and a consistent correlation between virus detection in stools and infection of volunteers. RT-PCR detected virus in stool samples diluted 10(-4) and was about 100 times more sensitive than dot blot hybridization. In serial stool samples collected before and at different times after inoculation of 10 volunteers with Norwalk virus, 37 of 55 were positive by RT-PCR, but only 27 were positive by dot blot hybridization (chi 2 = 22.96; P less than 0.001). Further application of this method should allow detection of Norwalk virus in food or environmental samples such as shellfish and shellfish waters.

Adult↗
PubMed1992

Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein.

Norwalk virus capsid protein was produced by expression of the second and third open reading frames of the Norwalk virus genome, using a cell-free translation system and baculovirus recombinants. Analysis of the expressed products showed that the second open reading frame encodes a protein with an apparent molecular weight of 58,000 (58K protein) and that this protein self-assembles to form empty viruslike particles similar to native capsids in size and appearance. The antigenicity of these particles was demonstrated by immunoprecipitation and enzyme-linked immunosorbent assays of paired serum samples from volunteers who developed illness following Norwalk virus challenge. These particles also induced high levels of Norwalk virus-specific serum antibody in laboratory animals following parenteral inoculation. A minor 34K protein was also found in infected insect cells. Amino acid sequence analysis of the N terminus of the 34K protein indicated that the 34K protein was a cleavage product of the 58K protein. The availability of large amounts of recombinant Norwalk virus particles will allow the development of rapid, sensitive, and reliable tests for the diagnosis of Norwalk virus infection as well as the implementation of structural studies.

Antibody Formation↗
PubMed1992

Expression of aminopeptidase N in fetal rat lung during development.

The ectopeptidase, aminopeptidase N, serves as a cell surface marker of the apical surface of the alveolar type II epithelial cell in adult lung. It is also present in fetal lung before differentiation of morphologically mature type II alveolar epithelial cells, suggesting that it is expressed by precursors of the type II cells. We have examined the mRNA coding for the aminopeptidase in adult and fetal lung and in mature type II cells and determined levels of mRNA and immunoreactive protein during fetal lung development. Comparison of the temporal patterns of steady-state levels of aminopeptidase mRNA and immunoreactive protein during development show that the expression of the protein is developmentally regulated and that expression is regulated, at least in part, at a pretranslational level. Both mRNA and immunoreactive protein levels increase severalfold on the final gestational day, suggesting that the function of the aminopeptidase may be associated with air breathing.

Aging↗
PubMed1992

[Hypolipodemic action of zhikeqing].

Zhikeqing is a compound prescription of Chinese herbs. It has been proved shown to possess an obvious effect on lipid and lipoprotein cholesterol contents in the serum of hyperlipidemic mice or rats.

Animals↗
PubMed1991

Concentration and detection of hepatitis A virus and rotavirus from shellfish by hybridization tests.

A modified polyethylene glycol precipitation method for concentration of virus followed by a new method to recover nucleic acid was used to detect hepatitis A virus (HAV) and rotavirus (SA11) in shellfish (oysters and hard-shell clams) by hybridization tests. Infectious virus, seeded into relatively large quantities of shellfish, was recovered consistently, with greater than 90% efficiency as measured by either in situ hybridization (HAV) or plaque assay (rotavirus SA11). Viral nucleic acid for dot blot hybridization assays was extracted and purified from virus-containing polyethylene glycol concentrates. Separation of shellfish polysaccharides from nucleic acid was necessary before viral RNA could be detected by dot blot hybridization. Removal of shellfish polysaccharides was accomplished by using the cationic detergent cetyltrimethylammonium bromide (CTAB). Use of CTAB reduced background interference with hybridization signals, which resulted in increased hybridization test sensitivity. After polysaccharide removal, dot blot hybridization assays could detect approximately 10(6) physical particles (corresponding to approximately 10(3) infectious particles) of HAV and 10(4) PFU of SA11 rotavirus present in 20-g samples of oyster and clam meats. These studies show continuing promise for the development of uniform methods to directly detect human viral pathogens in different types of shellfish. However, practical applications of such methods to detect noncultivatable human viral pathogens of public health interest will require additional improvements in test sensitivity.

Animals↗
PubMed1989

The transformation of the agricultural population and the urbanisation process in China.

"Nine causes for the occurrence of surplus agricultural labour with the modernisation of agriculture have been identified. Several solutions to this problem are presented. Urbanisation of the agricultural population in a way suited to China's characteristics is necessary, important and possible." An example of labor saving through mechanization in Heilongjiang province is presented.

Agriculture↗