Biomedical subjects

X Huang

Publications and source records attributed to X Huang.

At least 271 records · Page 15Linked to original sources

PubMed2000

[Middle-aged and elderly patients with hydronephrosis induced by ureteric obstruction: etiology and diagnosis].

OBJECTIVE: To improve the etiologic diagnosis of middle-aged and elderly patients with ureteric obstruction. METHODS: The etiology and diagnosis were retrospectively studied in 141 middle-aged and elderly patients with hydronephrosis in recent 10 years. RESULT: 59 patients had ureterolithiasis, 51 ureteric cancer, 11 ureteric tuberculosis, 9 UPJ stricture, 6 uretero-cyst and 5 ureteric polyp. CONCLUSIONS: Ureterolithiasis and ureteric cancer contribute greatly to ureteric obstruction in middle-aged and elderly patients. Etiologically, B ultrasonography is suggested to be used as the first choice in diagnosis and follow-up of the middle-aged and elderly patients with hydronephrosis, especially those accompanied with hematuria.

Aged↗

PubMed2000

[An experimental study on F-heparin surface modified IOLs implanted into the Rhesus monkeys' eyes].

OBJECTIVE: To evaluate the biocompatibility of F-heparin surface modified IOLs. METHODS: 13 monkeys were divided into 3 groups, and implanted with different surface modified and non-modified IOLs into their eyes. All of the eyes were examined by slitlamp microscope and Schiötz tonometer at postoperative 15, 30, 60, 90 and 180 days. Postoperatively, at different periods the aqueous was aspirated from the anterior chamber to calculate cells. RESULTS: F-heparin surface modified IOLs induced milder inflammatory reaction and less dense posterior capsula opacification (PCO) than non-modified IOLs. CONCLUSION: F-heparin surface modified IOL has a better biocompatibility.

Animals↗

PubMed2000

[Lung injury caused by passive smoking and its effects on cytokines in rats].

OBJECTIVE: A rat model with chronic bronchitis was replicated by passive inhalation of cigarette smoking fume to study its long-term effects on lung injury and nitric oxide (NO), interluekin-6 (IL-6), interleukin-8 (IL-8). METHODS: Levels of nitrogen dioxide (NO(2)) and nitrogen trioxide (NO(3)) were measured with spectrophotometry in rats indicating their level of nitric oxide (NO). Levels of IL-6 and IL-8 were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: Levels of NO in serum, bronchial alveolar lavage fluid (BALF) and lung tissue in the smoking group were significantly lower than those in the normal controls (P < 0.01). But, levels of IL-6 and IL-8 were higher in the smoking group than those in the controls. CONCLUSION: Long-term passive smoking could cause injury of lung tissue to certain extent, reduction in secretion of NO in endothelial cells and damage to pulmonary vessels.

Animals↗

PubMed2000

[The expression of P16, P21(WAF1/CIP1) and Cyclin D1 proteins in skin of patients with arseniasis caused by coal-burning and their significance].

OBJECTIVE: To study the relationships between arseniasis and skin carcinoma. METHODS: The expressions of p16, p21(WAF1/CIP1) and Cyclin D1 proteins in skin of arsenic intoxication patients caused by coal-burning was measured by immunohistochemistry methods. RESULTS: The intensity and density, and the positive percentage of p16 protein were gradually reduced as the skin pathological changes progressed. Expressions of p16 protein was lower in the carcinoma group (A group), pre-carcinoma group (B group) and overkeratosis group (C group) than the general cell proliferation group (D group) and normal group (P < 0.05, P < 0.01). On the other hand, the cell density and the positive percentage of p21(WAF1/CIP1) and Cyclin D1 proteins were gradually increased as the skin pathological changes progressed. The expression of p21(WAF1/CIP1) protein in all pathological groups were higher than the normal group (P < 0.05, P < 0.01), and expression of CyclinD1 protein in A, B and C groups were higher than the D group and normal group (P < 0.05, P < 0.01). CONCLUSIONS: Skin overkeratosis may be the earlier stage of skin carcinoma, lower expression of p16 protein and overexpression of Cyclin D1 protein may be considered as prognostic biomarkers to skin carcinogenesis; p16, p21(WAF1/CIP1) and Cyclin D1 may play important role in the cooperative way in the development of arseniasis caused by coal-burning and further progression to skin carcinoma. Monitoring of the expression of p16, p21(WAF1/CIP1) and Cyclin D1 proteins may be valuable for early detection and early treatment and prognostic assessment of skin carcinoma caused by coal-burning.

Arsenic Poisoning↗

PubMed2000

[Repairing severe deep burn wound with transplantation of the latissimus dorsi myocutaneous flap].

OBJECTIVE: To repair the deep wound caused by high voltage electric injury and hot mangle injury with the latissimus dorsi myocutaneous flaps. METHODS: Latissimus dorsi myocutaneous flaps were applied to repair deep burn wounds in 26 cases in which 25 were island flap transposition and 1 free flap transplantation. RESULTS: The biggest flap in this group was 40 cm x 20 cm. All flaps survived but one exception with tip necrosis of the flap. Reconstruction of the Achilles tenden was satisfactory. CONCLUSION: Latissimus dorsi myocutaneous flaps are the ideal choice for repairing local severe deep burn wounds.

Adult↗

PubMed2000

[Effect of serum and wound exudates on PMN apoptosis in burned patients].

OBJECTIVE: To study in vitro the effect of burn serum and wound exudates on PMN apoptosis in burned patients. METHODS: The burn serum was obtained from patients within 3 days postburn. The blister fluid was collected from intact blisters in burn wound within 24 hours postburn, and subeschar tissue fluid underneath III degree burn wound during escharectomy. PMNs were isolated from healthy human donors and cultured for 24 hours at 37 degreeC and 5% CO(2) with normal serum (NS), burn serum (BS), burn blister fluid (BBF) and subeschar tissue fluid (STF), respectively. Morphological assessment and quantification of PMN apoptosis were performed with AO + EB stain and flow cytometric analysis. RESULTS: The number of apoptotic PMN and DNA fragmentation of PMN significantly decreased (P < 0.05) when PMN were cultured with BS, BBF, and STF when compared with that with NS. In addition, the specific DNA "ladder" pattern for apoptotic cells couldn't be found on the agarose gel electrophoresis in this case. However, BBF exhibited weaker inhibiting effect than BS and STF on PMN apoptosis. CONCLUSION: PMN apoptosis could be inhibited in vitro by BS, STF and BBF from burned patients.

Apoptosis↗

PubMed2000

[Clinical observation on treatment of pediatric intractable nephropathy with modified taohong siwu decoction].

OBJECTIVE: To observe the clinical effect of Modified Taohong Siwu Decoction (MTSD) in treating pediatric intractable nephropathy (PIN). METHODS: Ninety-five cases of PIN were divided into 2 groups at random. The 60 cases in the treated group were administered with MTSD and the 35 patients in the control group were treated with heparin. The clinical therapeutic effect and levels of thromboxane B2(TXB2) and 6-keto-prostaglandin F1 alpha before and after treatment were observed. RESULTS: The total effective rate of the treated group was 81.7%, which was similar to that in the control group (80.0%, P > 0.05). The levels of TXB2 and TXB2/6-ketoprostaglandin F1 alpha ratio were higher in both groups of patients as compared with those of the healthy control (P < 0.01), After treatment, the two criteria were significantly improved in the two groups, as compared with those before treatment, the difference was significant (P < 0.01), while in comparson between the treated and the control group, no significant difference was found (P > 0.05). CONCLUSION: MTSD has good effect in treating PIN, it could improve the metabolic unbalance of thromboxane and prostacyclin.

6-Ketoprostaglandin F1 alpha↗

PubMed2000

[Observation of the modulatiny effect of histamine on intestinal barrier function].

The model of CaCo2 cell monolayer system has been generally accepted as a standard method for in vitro study of the relationship between the epithelium and microbial invasion. In this study, we set up the model with a simplified method and used it to observe if histamine, which is an important inflammatory factor in gastroenteric tract, can offer some barrier protection from E. coli invasion. After two weeks' routine incubation, the established CaCo2 cells monolayer systems were co-cultured with 1 x 10(-8) mmol/L, 1 x 10(-7) mmol/L and 1 x 10(-6) mmol/L histamine DMEM for 2 hours, and with frank DMEM as control for the same length of the time. For observation on time course effect of histamine, the co-cultures with 1 x 10(-6) mmol/L histamine DMEM were kept up for 0.5, 1.0, 2.0, 4.0 and 18.0 hours. Bacterial invasion ws assessed by quantitating the number of E. coli within the coultured epithelial cells. The results showed that histamine significantly inhibited the invasion of E. coli to epithelial cells(P < 0.05). The colony counts in co-cultures with 1 x 10(-7) mmol/L and 1 x 10(-8) mmol/L histamine DMEM were 52.5, 30.3 and 91.3 respectively, compared with 563 in control group. In the study of the time course effect of histamine, the colony conuts of co-culture with histamine DMEM for 0.5, 1.0, 2.0, 4.0 and 18.0 hours were 135.5, 64.0, 29.5, 36.0 and 22.5 respectively. It was concluded that histamine can enhance the protective barrier function of intestinal epithelium against E. coli invasion.

Bacterial Translocation↗

PubMed2000

[Preliminary echocardiographic evaluation on prosthetic valve replacement of preserving the subvalvular apparatus with artificial chorade for mitral stenosis].

The purpose of this study was to evaluate the preliminary results after prosthetic valve replacement of preserving the subvalvular apparatus with artificial chorade for mitral stenosis. 20 patients had received operation with expanded polytetrafluoroethylene(e-PTFE) as artificial chordade. The patients were followed up by means of echocardiography. The time of follow-up study varied from 4 to 18 months with a mean of 6 months. The left atrial, ventricular dimension as well as ventricular function were assessed. In addition, the length of artificial chorade and obstruction of left ventricular outflow track were observed. The results showed that left atrial diameter (LAD, from 57.59 +/- 13.51 mm to 47.05 +/- 9.55 mm), left ventricular end-diastolic dimension (LVEDD, from 46.23 +/- 12.56 mm to 42.41 +/- 10.86 mm), left ventricular end-systolic dimension (LVESD, from 33.50 +/- 10.20 mm to 29.68 +/- 8.40 mm) decreased (P < 0.01), and ejection fraction (EF, from 52.59 +/- 11.89% to 58.36 +/- 8.03%) increased (P < 0.05) after operation. There were no significant changes of fractional shortening (FS, from 29.23 +/- 8.99% to 31.05 +/- 6.08%). The length of artificial chorade ranged from 2.6 cm to 4.3 cm (the mean, 3.2 cm). There was no case of obstruction of left ventricular outflow track. It is believed that the result of preliminary echocardiographic observation on prosthetic valve replacement of preserving the subvalvular apparatus with artificial chorade for mitral stenosis is satisfactory.

Adult↗

PubMed2000

[Study on the effect of several antioxidants on the stability of oils].

The stability of oils is very important for ensuring the sanitation and quality of oils and the foods containing oils and fats. In this study, BHT, TBHQ and micro-capsule antioxidants (mixed antioxidants) were tested for their properties of antioxidation in oils under different temperatures. The results showed that the mixed antioxidant used in oils was more effective than the single antioxidant, especially under high temperature. It was concluded that microcapsule antioxidant was effective, economical and relatively safe when it was used in oils for frying foods.

Antioxidants↗

PubMed2000

[Evaluation of cariogenic potential of Streptococcus mutans isolated from caries-free and -active persons: adherence properties to saliva-coated hydroxyapatite].

OBJECTIVE: In this study, authors used saliva-coated hydroxyapatite to simulate tooth surface in oral cavity to evaluate adherence of different Streptococcus mutans isolated from caries-free and -active persons to saliva-coated hydroxyapatite. METHODS: Streptococcus mutans isolated from people with different carious experiences were divided into two groups: the caried-free group (DMFS = 0) including 19 persons and the caries-active group (DMFS > or = 6) including 40 persons. Totally 105 genotype Streptococcus mutans strains were obtains, including 22 strains from the caries-free group, and 83 strains from the caries-active group. The adhering properties of Streptococcus mutans (serotype c) strains were investigated, and different adhering properties were compared between the caries-free group and the caries-active group in order to find the correlationship between carious experiences and adhering properties of Streptococcus mutans. Saliva-coated hydroxyapatite was used to simulated tooth surface in oral cavity. Streptococci mutans were cultured in TPY liquid medium containing 3H-TDR in the same radioactive concentration. After being cultured in an anaerobic incubator for 18 hours, Streptococci mutans were radiolabelled by 3H-TDR. Saliva-coated hydroxyapatite and bacterial liquid(BSA = 0.52) were mixed for 90 minutes. Samples were assayed by using liquid scintillation counter, and binding abilities of strains were evaluated by the count per minute(cpm). RESULTS: Binding abilities of strains were significantly different in two groups, Caries-active people harbored more Streptococci mutans with high binding abilities(cpm > 2000) than caries-free people did(P < 0.05). The binding abilities to saliva-coated hydroxyapatite were different between strains of differing genotypes isolated from the same person. Each person always harbored one or two strains with high binding abilities(cpm > 2000) in the caries-active group. CONCLUSION: 1. Different genotypes have different adhering properties. 2. In caries-active group, different genotype strains have synergic effects, and adhesion of high binding ability strains cause caries susceptibility. 3. The differences in binding properties of isolates might relate to differences in carious experiences.

Bacterial Adhesion↗

PubMed2000

[Evaluation of cariogenic potential of Streptococcus mutans isolated from caries-free and -active persons: abilities to synthesize water-soluble and -insoluble glucans].

OBJECTIVE: In this study, authors investigated abilities of Streptococcus mutans (serotype c) strains to synthesize water-soluble and water-insoluble glucans. METHODS: Streptococcus mutans strains were isolated from people with different carious experiences, which were divided into two groups: caries-free (DMFS = 0) group including 19 persons and caries-active group(DMFS > or = 6) including 40 persons. Totally 105 genotypes of Streptococcus mutans strains were obtained, including 22 strains from the caries-free group and 83 strains from the caries-active group. The differences of abilities to synthesize water-soluble and water-insoluble glucans between these two groups were compared in order to find the correlationship between the synthesis of glucans and caries experience. Then, Streptococcus mutans were cultured in TPY liquid medium containing 1% sucrose in an anaerobic incubator at 37 degrees C for 24 hours. Glucans synthesized by Streptococcus mutans was qualified by using infrared spectrophotometry. The amounts of water-soluble and water-insoluble glucans were measured by using the anthron method. RESULTS: According to the same absorption tops position analysed by infrared spectrophotometry, glucans samples were certified. The amounts of glucans synthesized by Streptococcus mutans strains were different between strains of different genotypes even isolated from the same person. Mostly, the amount of water-insoluble glucans was higher than that of water-soluble glucans between different genotype Streptococcus mutans strains. The oral environments of patients in the caries-active group harbored more Streptococcus mutans strains with higher water-soluble glucans producing ability than those of the caries-free group did (P < 0.05), and patients of the caries-active group also harbored more Streptococcus mutans strains with higher water-insoluble glucans producing ability than those of the caries-free group did(P < 0.01). CONCLUSION: 1. Glucosyltranferases of different genotype strains are different in glucans production, and different genotype strains relate to different cariogenic abilities. 2. Glucans was one of cariogenic factors, and water-insoluble glucans was more important than water-soluble glucans in cariogenic ability of Streptococcus mutans. 3. The differences in glucans production of isolated strains might relate to differences in carious experiences.

Dental Caries↗

PubMed2000

[The proliferating cells in the rat endolymphatic sac].

OBJECTIVE: To study the cellular proliferating activity of the endolymphatic sac. METHOD: 10 healthy adult S-D rats were used for the experiment. After administration of 5-bromo-2'-deoxyuridine (BrdUrd) intraperitoneally, the rats were sacrificed and the temporal bones were removed, then the decalcified samples examined immunohistochemically by using monoclonal antibodies against BrdUrd. RESULT: Scattered BrdUrd+ cells were detected within the endolymrhatic sac (ES) whereas labelled cells were not fonnd in the cochlea. CONCLUSION: Possessing a low rat of mitotic activity, the ES may be an important the sole site for the cell-renewing in the inner ear.

Animals↗

PubMed2000

[Study on the biology feature and pathogenicities to silkworm of a microsporidium isolated from Barathra brassicae L].

A microsporidium (called as Bab-M) was isolated from Barathra brassicae L. captured from suburban vegetable plot of Guang Zhou. The spores were long-ovoid in shape and 4.02 +/- 0.36 microns x 1.99 +/- 0.36 microns in size. Immunologically the microsporidium shared spore surface specific antigen(s) with N. bombycis. The ultrastructure and life cycle of Bab-M were similar to that of N. b.. The rate of transovarian transmission was high. The initial conclusion was that Bab-M should be referred to as Nosema bombycis, but there was variation between them.

Animals↗

PubMed2000

[Studies on the supercritical CO2 fluid extraction and separation of psoralen, isopsoralen and fatty oils from Psoralea corylifolia].

Opimum extraction conditions were studied by supercritical carbon dioxide fluid extraction technology and orthogonal tests. Crystal of psoralen can be achieved directly from the extractives. Magnified experiments were carried out. The chemical constituents of fatty oils were analyzed by GC-MS. The result shows that the optimum conditions are: extraction pressure 27 MPa, extraction temperature 70 degrees C; seperation pressure 9 MPa, seperation temperature 60 degrees C. Six components such as oleic acid, linoleic acid etc. were identified in the fatty oils.

Carbon Dioxide↗

PubMed2000

[Determination of glycyrrihizin in rats plasma and pharmacokinetics after oral administration of si-jun-zi-tang].

OBJECTIVE: To study the pharmacokinetics of glycyrrhizin after oral administration of Si-jun-zi-tang in rats and provide the experimental evidences for the prescription pharmacokientics of traditional Chinese medicine. METHOD: A RP-HPLC method was established for rapid determination of glycyrrhizin in rats plasma. After obtaining the blood sample at different time, the plasma concentration of GL were detected respectively. The data were processed by the software named 3P87 to find the pharmacokinetic parameters. RESULTS: A good linear relationship existed in the range of GL from 1.224 micrograms/ml to 38.656 micrograms/ml. Whole blood concentration-time course of GL after oral administration Si-jun-zi-tang was fitted to be a two-compartment model. GL in rats plasma which came from Si-jun-zi-tang was absorbed and distributed rapidly, but excreted slowly. CONCLUSION: This RP-HPLC method was sensitive, raliable and rapid, it can be used in absorption and metabolism of GL, also in detection of GL preparation. This experiment is prepared for further study of the tradtional Chinese medicine prescription pharmacokinetics.

Administration, Oral↗

PubMed2000

Bifunctional effect of human IFN-gamma on cultured human fibroblasts from Tenon's capsule.

PURPOSE: To study the effect of human IFN-gamma on in vitro cultured human fibroblasts from Tenon's capsule. MATERIALS AND METHODS: The effect of different concentrations of human IFN-gamma and mitomycin-C (MMC), 5-fluorouracil (5-Fu) on cultured human Tenon's capsule fibroblasts (HTCF) was measured using a MTT [3-(4, 5-dimethylthiazo-2-yl)]-2, 5-diphenyltetrazolium bromide; Thiazolyl blue) colorimetric assay. The results were analyzed using ANOVA of the statistical package for social sciences (SPSS) 9.0 version. The difference was considered to be significant if P < 0.05. RESULTS: The effects of MMC and 5-Fu on the growth of HTCF were negative, while the effects of IFN-gamma on the growth of HTCF were both negative (10(2)-10(4) units/ml in two experiments) and positive (10(6), 10(5), 10 units/ml in two experiments). The inhibition rate of MMC ranged from 5.73% to 46.9%, which was similar to the inhibition rate of 5-Fu ranged from 12.49% to 38.92% (P = 0.351). The inhibition rate of IFN-gamma in two experiments was smaller than MMC and 5-Fu (P < 0.05). CONCLUSION: IFN-gamma has bifunctional effect (both enhancement and inhibition) on proliferation of cultured HTCF. The antiproliferative effect of IFN-gamma was weaker than MMC and 5-Fu. Further study has to be carried out to document the inhibition of scar formation of filtration bleb by IFN-gamma and the molecular mechanisms of its bifunctional effect on HTCF proliferation.

Cell Division↗

PubMed2000

[Expression of heat shock protein 70 in immune response of the guinea pig inner ear].

OBJECTIVE: To explore whether the immune response of the inner ear could induce heat shock protein (hsp) 70 in guinea pig cochlea. METHODS: A model of autoimmune inner ear disease (AIED) was established by systemically immunizing the guinea pig with the homologous crude inner ear antigen (CIEAg). The immunized cochleae and normal control cochleae were examined for the expression of hsp70 with techniques of immunohistochemistry and in situ hybridization. RESULTS: In the control animals, the expression of the hsp70-like protein appeared only in the spiral ganglion, whereas in the cochleae with CIEAg immunization, strong expression of the hsp70-like protein and its mRNA appeared in the spiral ganglion as well as in the stria vascularis and the spiral ligament. The hearing thresholds were significantly increased in 10 out of 28 cochleae (35.7%) with CIEAg immunization. CONCLUSION: The results suggest that the immune response of the inner ear can induce the expression of hsp70 in the guinea pig cochlea.

Animals↗